Reviewed, UniProtKB/Swiss-Prot Q9Z6M4 (ARODE_CHLPN)

Names and origin

Protein names

Recommended name:
    Shikimate biosynthesis protein aroDE
Including the following 2 domains:
    1- Recommended name:
            3-dehydroquinate dehydratase
                Short name=3-dehydroquinase
              EC=4.2.1.10
        Alternative name(s):
            Type I DHQase
    2- Recommended name:
            Shikimate dehydrogenase
              EC=1.1.1.25

Gene names

Name:	aroE
Synonyms:	aroD/E
Ordered Locus Names:	CPn_1035, CP_0817, CpB1075

Organism

Chlamydia pneumoniae (Chlamydophila pneumoniae) [Complete proteome] [HAMAP]

Taxonomic identifier

83558 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydophila

Protein attributes

Sequence length	477 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes two sequential steps of the aromatic amino acids biosynthetic pathway. The first reaction is catalyzed by the 3-dehydroquinate dehydratase, coded by the aroD domain; the second reaction is catalyzed by the shikimate 5-dehydrogenase, coded by the aroE domain. HAMAP MF_00214
Catalytic activity	3-dehydroquinate = 3-dehydroshikimate + H2O. HAMAP MF_00214 Shikimate + NADP+ = 3-dehydroshikimate + NADPH. HAMAP MF_00214
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7. HAMAP MF_00214 Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7.
Sequence similarities	In the N-terminal section; belongs to the type-I 3-dehydroquinase family. In the C-terminal section; belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NADP Schiff base
Molecular function	Lyase Oxidoreductase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: HAMAP NADP or NADPH binding Inferred from electronic annotation. Source: InterPro shikimate 5-dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 477	477	Shikimate biosynthesis protein aroDE HAMAP MF_00214		PRO_0000138823
Regions
	Nucleotide binding	339 – 343	5	NADP By similarity
	Region	1 – 209	209	3-dehydroquinate dehydratase HAMAP MF_00214
	Region	210 – 477	268	Shikimate 5-dehydrogenase HAMAP MF_00214
Sites
	Active site	111	1	Proton acceptor By similarity
	Active site	134	1	Schiff-base intermediate with substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Z6M4-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 3B57DD86C163E935	FASTA	477	52,945
	10 20 30 40 50 60 MLCATVSGPS FCEAKQQILK SLHLVDIIEL RLDLINELDD QELHTLITTA QNPILTFRQH 70 80 90 100 110 120 KEMSTALWIQ KLYSLAKLEP KWMDIDVSLP KTALQTIRKS HPKIKLILSY HTDKNEDLDA 130 140 150 160 170 180 IYNEMLATPA EIYKIVLSPE NSSEALNYIK KARLLPKPST VLCMGTHGLP SRVLSPLISN 190 200 210 220 230 240 AMNYAAGISA PQVAPGQPKL EELLSYNYSK LSEKSHIYGL IGDPVDRSIS HLSHNFLLSK 250 260 270 280 290 300 LSLNATYIKF PVTIGEVVTF FSAIRDLPFS GLSVTMPLKT AIFDHVDALD ASAQLCESIN 310 320 330 340 350 360 TLVFRNQKIL GYNTDGEGVA KLLKQKNISV NNKHIAIVGA GGAAKAIAAT LAMQGANLHI 370 380 390 400 410 420 FNRTLSSAAA LATCCKGKAY PLGSLENFKT IDIIINCLPP EVTFPWRFPP IVMDINTKPH 430 440 450 460 470 PSPYLERAQK HGSLIIHGYE MFIEQALLQF ALWFPDFLTP ESCDSFRNYV KNFMAKV

References

[1]	"Comparative genomes of Chlamydia pneumoniae and C. trachomatis." Kalman S., Mitchell W.P., Marathe R., Lammel C.J., Fan J., Hyman R.W., Olinger L., Grimwood J., Davis R.W., Stephens R.S. Nat. Genet. 21:385-389(1999) [PubMed: 10192388] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CWL029.
[2]	"Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39." Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O., Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S., Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J., Gwinn M.L. , Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G., Salzberg S.L., Eisen J.A., Fraser C.M. Nucleic Acids Res. 28:1397-1406(2000) [PubMed: 10684935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AR39.
[3]	"Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA." Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T. Nucleic Acids Res. 28:2311-2314(2000) [PubMed: 10871362] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J138.
[4]	"The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis." Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TW-183.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001363 Genomic DNA. Translation: AAD19172.1. AE002161 Genomic DNA. Translation: AAF38612.1. BA000008 Genomic DNA. Translation: BAA99242.1. AE009440 Genomic DNA. Translation: AAP99004.1. Different initiation.
PIR	G72003. H86619.
RefSeq	NP_225229.1. NP_301090.1. NP_445356.1. NP_877347.2.
3D structure databases
SMR	Q9Z6M4. Positions 1-460, 211-474.
ModBase	Search...
Genome annotation databases
GeneID	1467754. 894704. 919809. 962771.
GenomeReviews	Gene locus CPn_1035 in contig AE001363_GR. Gene locus CP_0817 in contig AE002161_GR. Gene locus CpB1075 in contig AE009440_GR. Gene locus aroE in contig BA000008_GR.
KEGG	cpa:CP0817. cpn:CPn1035. cpt:CpB1075.
TIGR	CP_0817.
Phylogenomic databases
HOGENOM	HBG414053.
Enzyme and pathway databases
BioCyc	CPNE115711:CP_0817-MONOMER. CPNE115713:CPN1035-MONOMER. CPNE138677:CPJ1035-MONOMER. CPNE182082:CPB1075-MONOMER.
BRENDA	1.1.1.25. 264832. 4.2.1.10. 264832.
Family and domain databases
HAMAP	MF_00214. AroD. Fused. [Tree] MF_00222. Shikimate_DH_AroE. Fused. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR001381. DHquinase_I. IPR016040. NAD(P)-bd_dom. IPR011342. Quinate/shikimate_5-DH. IPR013708. Shikimate_DH-bd_N. IPR006151. Shikm_DH/Glu-tRNA_Rdtase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01487. DHquinase_I. 1 hit. PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00507. aroE. 1 hit.
PROSITE	PS01028. DEHYDROQUINASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARODE_CHLPN

Accession

Primary (citable) accession number: Q9Z6M4
Secondary accession number(s): Q9JQ99

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	May 1, 1999
Last modified:	February 9, 2010
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)