Q9Z6L7 (BIOD_CHLPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3

Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase

Gene names

Name:	bioD
Ordered Locus Names:	CPn_1042, CP_0810, CpB1082

Organism

Chlamydia pneumoniae (Chlamydophila pneumoniae) [Complete proteome] [HAMAP]

Taxonomic identifier

83558 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydia/Chlamydophila group › Chlamydia

Protein attributes

Sequence length	219 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 219	219	ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336		PRO_0000187956
Regions
	Nucleotide binding	96 – 99	4	ATP By similarity
Sites
	Metal binding	16	1	Magnesium 2 By similarity
	Metal binding	43	1	Magnesium 2 By similarity
	Metal binding	96	1	Magnesium 2 By similarity
	Binding site	43	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Z6L7 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: A207D40FF11234B4	FASTA	219	24,165
	10 20 30 40 50 60 MQRIIIVGID TGVGKTIVSA ILARALNAEY WKPIQAGNLE NSDSNIVHEL SGAYCHPEAY 70 80 90 100 110 120 RLHKPLSPHK AAQIDNVSIE ESHICAPKTT SNLIIETSGG FLSPCTSKRL QGDVFSSWSC 130 140 150 160 170 180 SWILVSQAYL GSINHTCLTV EAMRSRNLNI LGMVVNGYPE DEEHWLTQEI KLPIIGTLAK 190 200 210 EKEITKTIIS CYAEQWKEVW TSNHQGIQGV SGTPSLNLH

References

[1]	"Comparative genomes of Chlamydia pneumoniae and C. trachomatis." Kalman S., Mitchell W.P., Marathe R., Lammel C.J., Fan J., Hyman R.W., Olinger L., Grimwood J., Davis R.W., Stephens R.S. Nat. Genet. 21:385-389(1999) [PubMed: 10192388] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CWL029.
[2]	"Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39." Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O., Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S., Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J., Gwinn M.L. , Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G., Salzberg S.L., Eisen J.A., Fraser C.M. Nucleic Acids Res. 28:1397-1406(2000) [PubMed: 10684935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AR39.
[3]	"Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA." Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T. Nucleic Acids Res. 28:2311-2314(2000) [PubMed: 10871362] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J138.
[4]	"The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis." Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TW-183.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001363 Genomic DNA. Translation: AAD19179.1. AE002161 Genomic DNA. Translation: AAF38607.1. BA000008 Genomic DNA. Translation: BAA99249.1. AE009440 Genomic DNA. Translation: AAP99011.1.
PIR	F72004. G86620.
RefSeq	NP_225236.1. NC_000922.1. NP_301097.1. NC_002491.1. NP_445349.1. NC_002179.2. NP_877354.1. NC_005043.1.
3D structure databases
ProteinModelPortal	Q9Z6L7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1467761. 895031. 919814. 962749.
GenomeReviews	Gene locus CPn_1042 in contig AE001363_GR. Gene locus CP_0810 in contig AE002161_GR. Gene locus CpB1082 in contig AE009440_GR. Gene locus bioD in contig BA000008_GR.
KEGG	cpa:CP0810. cpn:CPn1042. cpt:CpB1082.
TIGR	CP_0810.
Phylogenomic databases
HOGENOM	HBG650065.
OMA	WKPIQSG.
ProtClustDB	CLSK399497.
Enzyme and pathway databases
BioCyc	CPNE115711:CP_0810-MONOMER. CPNE115713:CPN1042-MONOMER. CPNE138677:CPJ1042-MONOMER. CPNE182082:CPB1082-MONOMER.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR004472. DTB_synth_BioD. [Graphical view]
KO	K01935.
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_CHLPN

Accession

Primary (citable) accession number: Q9Z6L7

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	May 1, 1999
Last modified:	January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program