Reviewed, UniProtKB/Swiss-Prot Q9Z6K9 (DAPA_CHLPN)

Names and origin

Protein names

Recommended name:
    Dihydrodipicolinate synthase
      Short name=DHDPS
    EC=4.2.1.52

Gene names

Name:	dapA
Ordered Locus Names:	CPn_1050, CP_0802, CpB1091

Organism

Chlamydia pneumoniae (Chlamydophila pneumoniae) [Complete proteome] [HAMAP]

Taxonomic identifier

83558 [NCBI]

Taxonomic lineage

Bacteria › Chlamydiae › Chlamydiales › Chlamydiaceae › Chlamydophila

Protein attributes

Sequence length	291 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity. HAMAP MF_00418
Subcellular location	Cytoplasm By similarity HAMAP MF_00418.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 291	291	Dihydrodipicolinate synthase HAMAP MF_00418		PRO_0000103100
Sites
	Active site	157	1	Schiff-base intermediate with substrate By similarity
	Site	129	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Z6K9-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: BD6349DFC2924482	FASTA	291	32,317
	10 20 30 40 50 60 MHLLTATVTP FFPNGTIDFA SLERLLSFQD AVGNGVVLLG STGEGLSLTK KEKQALICFA 70 80 90 100 110 120 CDLQLKVPLF VGTSGTLLEE VLDWIHFCND LPISGFLMTT PIYTKPKLCG QILWFEAVLN 130 140 150 160 170 180 AAKHPAILYN IPSRAATPLY LDTVKALAHH PQFLGIKDSG GSVEEFQSYK SIAPHIQLYC 190 200 210 220 230 240 GDDVFWSEMA ACGAHGLISV LSNAWPEEAR EYVLNPQEQD YRSLWMETCR WVYTTTNPIG 250 260 270 280 290 IKAILAYKKA ITHAQLRLPL SIEDFDLENV SPAVESMLAW PKLRTSVFSY S

References

[1]	"Comparative genomes of Chlamydia pneumoniae and C. trachomatis." Kalman S., Mitchell W.P., Marathe R., Lammel C.J., Fan J., Hyman R.W., Olinger L., Grimwood J., Davis R.W., Stephens R.S. Nat. Genet. 21:385-389(1999) [PubMed: 10192388] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CWL029.
[2]	"Genome sequences of Chlamydia trachomatis MoPn and Chlamydia pneumoniae AR39." Read T.D., Brunham R.C., Shen C., Gill S.R., Heidelberg J.F., White O., Hickey E.K., Peterson J.D., Utterback T.R., Berry K.J., Bass S., Linher K.D., Weidman J.F., Khouri H.M., Craven B., Bowman C., Dodson R.J., Gwinn M.L. , Nelson W.C., DeBoy R.T., Kolonay J.F., McClarty G., Salzberg S.L., Eisen J.A., Fraser C.M. Nucleic Acids Res. 28:1397-1406(2000) [PubMed: 10684935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AR39.
[3]	"Comparison of whole genome sequences of Chlamydia pneumoniae J138 from Japan and CWL029 from USA." Shirai M., Hirakawa H., Kimoto M., Tabuchi M., Kishi F., Ouchi K., Shiba T., Ishii K., Hattori M., Kuhara S., Nakazawa T. Nucleic Acids Res. 28:2311-2314(2000) [PubMed: 10871362] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: J138.
[4]	"The genome sequence of Chlamydia pneumoniae TW183 and comparison with other Chlamydia strains based on whole genome sequence analysis." Geng M.M., Schuhmacher A., Muehldorfer I., Bensch K.W., Schaefer K.P., Schneider S., Pohl T., Essig A., Marre R., Melchers K. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: TW-183.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE001363 Genomic DNA. Translation: AAD19187.1. AE002161 Genomic DNA. Translation: AAF38601.1. BA000008 Genomic DNA. Translation: BAA99257.1. AE009440 Genomic DNA. Translation: AAP99020.1.
PIR	A72003. G86621.
RefSeq	NP_225244.1. NP_301105.1. NP_445341.1. NP_877363.1.
3D structure databases
SMR	Q9Z6K9. Positions 3-261.
ModBase	Search...
Genome annotation databases
GeneID	1467770. 894676. 919821. 962712.
GenomeReviews	Gene locus CPn_1050 in contig AE001363_GR. Gene locus CP_0802 in contig AE002161_GR. Gene locus CpB1091 in contig AE009440_GR. Gene locus dapA in contig BA000008_GR.
KEGG	cpa:CP0802. cpn:CPn1050. cpt:CpB1091.
TIGR	CP_0802.
Phylogenomic databases
HOGENOM	HBG358848.
OMA	CMLYNVP.
Enzyme and pathway databases
BioCyc	CPNE115711:CP_0802-MONOMER. CPNE115713:CPN1050-MONOMER. CPNE138677:CPJ1050-MONOMER. CPNE182082:CPB1091-MONOMER.
BRENDA	4.2.1.52. 264832.
Family and domain databases
HAMAP	MF_00418. DapA. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005263. DapA_synth. IPR002220. Dihydrodipicolinate_synth. IPR020625. Dihydrodipicolinate_synth_AS. IPR020624. Dihydrodipicolinate_synth_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PRINTS	PR00146. DHPICSNTHASE.
TIGRFAMs	TIGR00674. dapA. 1 hit.
PROSITE	PS00665. DHDPS_1. 1 hit. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA_CHLPN

Accession

Primary (citable) accession number: Q9Z6K9

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	May 1, 1999
Last modified:	February 9, 2010
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)