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Q9Z6C3

- PFKA_ENTCL

UniProt

Q9Z6C3 - PFKA_ENTCL

Protein

ATP-dependent 6-phosphofructokinase

Gene

pfkA

Organism
Enterobacter cloacae
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 72 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

    Catalytic activityi

    ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Allosterically activated by ADP and other diphosphonucleosides, and allosterically inhibited by phosphoenolpyruvate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei12 – 121ATP; via amide nitrogenUniRule annotation
    Metal bindingi104 – 1041Magnesium; catalyticUniRule annotation
    Active sitei128 – 1281Proton acceptorUniRule annotation
    Binding sitei155 – 1551Allosteric activator ADPUniRule annotation
    Binding sitei163 – 1631Substrate; shared with dimeric partnerUniRule annotation
    Binding sitei212 – 2121Allosteric activator ADPUniRule annotation
    Binding sitei223 – 2231SubstrateUniRule annotation
    Binding sitei244 – 2441Substrate; shared with dimeric partnerUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi73 – 742ATPUniRule annotation
    Nucleotide bindingi103 – 1064ATPUniRule annotation

    GO - Molecular functioni

    1. 6-phosphofructokinase activity Source: UniProtKB-HAMAP
    2. ATP binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fructose 6-phosphate metabolic process Source: InterPro
    2. glycolytic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Glycolysis

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00109; UER00182.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
    Short name:
    ATP-PFKUniRule annotation
    Short name:
    PhosphofructokinaseUniRule annotation
    Alternative name(s):
    PhosphohexokinaseUniRule annotation
    Gene namesi
    Name:pfkAUniRule annotation
    OrganismiEnterobacter cloacae
    Taxonomic identifieri550 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEnterobacterEnterobacter cloacae complex

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. 6-phosphofructokinase complex Source: InterPro

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 320320ATP-dependent 6-phosphofructokinasePRO_0000111953Add
    BLAST

    Proteomic databases

    PRIDEiQ9Z6C3.

    Interactioni

    Subunit structurei

    Homotetramer.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z6C3.
    SMRiQ9Z6C3. Positions 1-320.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni22 – 265Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
    Regioni55 – 606Allosteric activator ADP binding; shared with dimeric partnerUniRule annotation
    Regioni126 – 1283Substrate bindingUniRule annotation
    Regioni170 – 1723Substrate bindingUniRule annotation
    Regioni186 – 1883Allosteric activator ADP bindingUniRule annotation
    Regioni214 – 2163Allosteric activator ADP bindingUniRule annotation
    Regioni250 – 2534Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Prokaryotic clade "B1" sub-subfamily.UniRule annotation

    Family and domain databases

    HAMAPiMF_00339. Phosphofructokinase_I_B1.
    InterProiIPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view]
    PfamiPF00365. PFK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSiPR00476. PHFRCTKINASE.
    SUPFAMiSSF53784. SSF53784. 1 hit.
    TIGRFAMsiTIGR02482. PFKA_ATP. 1 hit.
    PROSITEiPS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9Z6C3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIKKIGVLTS GGDAPGMNAA IRGVVRAALT EGLEVFGVYD GYLGLYEDRM    50
    VQLDRYSVSD MINRGGTFLG SARFPEFRDE HVREVAIENM KKRGLDALVV 100
    IGGDGSYMGA KRLTEMGFPC IGLPGTIDND IKGTDYTIGF FTALGTVVEA 150
    IDRLRDTSSS HQRISIVEVM GRYCGDLTLA AAIAGGCEFI VVPEVEFSRE 200
    DLVAEIKAGI AKGKKHAIVA ITEHICDVDE LAKYIEAETK RETRATVLGH 250
    IQRGGSPGPY DRILASRMGA YAIDLLLQGH GGRCVGIQNE KLVHHDIIDA 300
    IENMKRPFKG DWLDCAKKLY 320
    Length:320
    Mass (Da):34,857
    Last modified:May 1, 1999 - v1
    Checksum:iCCF41BDD98EE98A1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF098509 Genomic DNA. Translation: AAD16179.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF098509 Genomic DNA. Translation: AAD16179.1 .

    3D structure databases

    ProteinModelPortali Q9Z6C3.
    SMRi Q9Z6C3. Positions 1-320.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi Q9Z6C3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00109 ; UER00182 .

    Family and domain databases

    HAMAPi MF_00339. Phosphofructokinase_I_B1.
    InterProi IPR012003. ATP_PFK_prok.
    IPR012828. PFKA_ATP.
    IPR022953. Phosphofructokinase.
    IPR015912. Phosphofructokinase_CS.
    IPR000023. Phosphofructokinase_dom.
    [Graphical view ]
    Pfami PF00365. PFK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000532. ATP_PFK_prok. 1 hit.
    PRINTSi PR00476. PHFRCTKINASE.
    SUPFAMi SSF53784. SSF53784. 1 hit.
    TIGRFAMsi TIGR02482. PFKA_ATP. 1 hit.
    PROSITEi PS00433. PHOSPHOFRUCTOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Role of pfkA and general carbohydrate catabolism in seed colonization by Enterobacter cloacae."
      Roberts D.P., Dery P.D., Yucel I., Buyer J., Holtman M.A., Kobayashi D.Y.
      Appl. Environ. Microbiol. 65:2513-2519(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: A-11 / 501R3.

    Entry informationi

    Entry nameiPFKA_ENTCL
    AccessioniPrimary (citable) accession number: Q9Z6C3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 21, 2001
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 72 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3