Q9Z671 (TYSY_ZYMMO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidylate synthase
Short name=TS
Short name=TSase
EC=2.1.1.45

Gene names

Name:	thyA
Synonyms:	thyB
Ordered Locus Names:	ZMO1755

Organism

Zymomonas mobilis subsp. mobilis (strain ATCC 31821 / ZM4 / CP4) [Complete proteome] [HAMAP]

Taxonomic identifier

264203 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Zymomonas › Zymomonas mobilis › Zymomonas mobilis subsp. mobilis

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008
Catalytic activity	5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008
Pathway	Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.
Sequence caution	The sequence AAD19406.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	dTMP biosynthetic process Inferred from electronic annotation. Source: HAMAP dTTP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	thymidylate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	Thymidylate synthase HAMAP-Rule MF_00008		PRO_0000141053
Sites
	Active site	177	1	By similarity
Experimental info
	Sequence conflict	206 – 215	10	LPFNLVGAAA → AAFQFGRRGR in AAD19406. Ref.1
	Sequence conflict	250 – 260	11	EQLAREPRAFP → NSLPVTSGFS in AAD19406. Ref.1
	Sequence conflict	276 – 287	12	IEDFA…GYDPH → SRILRLQDMILI in AAD19406. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9Z671 [UniParc]. Last modified February 15, 2005. Version 3. Checksum: 9D5A049C751A317A	FASTA	296	33,586
	10 20 30 40 50 60 MSEHQYLRLV SDILEKGDQR HDRTGVGTLS LFGAMMRFDL SKGRIPILTT KKVSYRLAIR 70 80 90 100 110 120 EMLWFLSGDT NIRPLVEQGV SIWSDWPLAR YQAETGALLS KKEFEAKILA DTEFAKKWGD 130 140 150 160 170 180 LGPVYGRQWR RWQGSDGQVY DQIATLIETL KSNPSSRRML FHGWNVAELK DMALPPCHMV 190 200 210 220 230 240 YQYHVTSDGR LNSLLYQRSA DVFLGLPFNL VGAAALQAML ADQAGLALGD LVWTGGDVHI 250 260 270 280 290 YRNHIDQMKE QLAREPRAFP RLELTRHPNS ITDYKIEDFA ITGYDPHPPI KGAVAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Um H.W., Kang H.S. Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 31821 / ZM4 / CP4.
[2]	"The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4." Seo J.-S., Chong H., Park H.S., Yoon K.-O., Jung C., Kim J.J., Hong J.H., Kim H., Kim J.-H., Kil J.-I., Park C.J., Oh H.-M., Lee J.-S., Jin S.-J., Um H.-W., Lee H.-J., Oh S.-J., Kim J.Y. , Kang H.L., Lee S.Y., Lee K.J., Kang H.S. Nat. Biotechnol. 23:63-68(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 31821 / ZM4 / CP4.
[3]	"Improved genome annotation for Zymomonas mobilis." Yang S., Pappas K.M., Hauser L.J., Land M.L., Chen G.L., Hurst G.B., Pan C., Kouvelis V.N., Typas M.A., Pelletier D.A., Klingeman D.M., Chang Y.J., Samatova N.F., Brown S.D. Nat. Biotechnol. 27:893-894(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF102543 Genomic DNA. Translation: AAD19406.1. Different initiation. AE008692 Genomic DNA. Translation: AAV90379.2.
RefSeq	YP_163490.2. NC_006526.2.
3D structure databases
ProteinModelPortal	Q9Z671.
ModBase	Search...
Protein-protein interaction databases
STRING	264203.ZMO1755.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAV90379; AAV90379; ZMO1755.
GeneID	3189297.
KEGG	zmo:ZMO1755.
PATRIC	32568778. VBIZymMob102260_1664.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0207.
HOGENOM	HOG000257899.
KO	K00560.
ProtClustDB	CLSK2822320.
Enzyme and pathway databases
UniPathway	UPA00575.
Family and domain databases
Gene3D	3.30.572.10. 1 hit.
HAMAP	MF_00008. Thymidy_synth_bact.
InterPro	IPR023451. Thymidate_synth/dCMP_Mease. IPR000398. Thymidylate_synthase. IPR020940. Thymidylate_synthase_AS. [Graphical view]
Pfam	PF00303. Thymidylat_synt. 1 hit. [Graphical view]
PRINTS	PR00108. THYMDSNTHASE.
SUPFAM	SSF55831. Thymidylat_synth_C. 1 hit.
TIGRFAMs	TIGR03284. thym_sym. 1 hit.
PROSITE	PS00091. THYMIDYLATE_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TYSY_ZYMMO

Accession

Primary (citable) accession number: Q9Z671
Secondary accession number(s): Q5NLN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	February 15, 2005
Last modified:	May 1, 2013
This is version 90 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program