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Protein

Signal transduction histidine-protein kinase/phosphatase MprB

Gene

mprB

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Member of the two-component regulatory system MprB/MprA which contributes to maintaining a balance among several systems involved in stress resistance and is required for establishment and maintenance of persistent infection in the host. In response to environmental signals MprB acts as both a membrane-associated protein kinase that undergoes autophosphorylation and subsequently transfers the phosphate to MprA, and a protein phosphatase that dephosphorylates phospho-MprA (By similarity).By similarity

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.

Cofactori

Mg2+By similarity, Mn2+By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Kinase, Protein phosphatase, Transferase

Keywords - Biological processi

Stress response, Two-component regulatory system, Virulence

Keywords - Ligandi

ATP-binding, Magnesium, Manganese, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Signal transduction histidine-protein kinase/phosphatase MprB (EC:2.7.13.3, EC:3.1.3.-)
Alternative name(s):
Mycobacterial persistence regulator B
Gene namesi
Name:mprB
Ordered Locus Names:ML0175
ORF Names:MLCB373.27
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML0175.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2626CytoplasmicSequence analysisAdd
BLAST
Transmembranei27 – 4721HelicalSequence analysisAdd
BLAST
Topological domaini48 – 163116ExtracellularSequence analysisAdd
BLAST
Transmembranei164 – 18421HelicalSequence analysisAdd
BLAST
Topological domaini185 – 519335CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 519519Signal transduction histidine-protein kinase/phosphatase MprBPRO_0000308434Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei249 – 2491Phosphohistidine; by autocatalysisPROSITE-ProRule annotation

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

STRINGi272631.ML0175.

Structurei

3D structure databases

ProteinModelPortaliQ9Z5G7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini186 – 23853HAMPPROSITE-ProRule annotationAdd
BLAST
Domaini246 – 466221Histidine kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HAMP domain.PROSITE-ProRule annotation
Contains 1 histidine kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG41062D9. Bacteria.
COG0642. LUCA.
HOGENOMiHOG000223177.
KOiK07653.
OMAiQEMVELR.
OrthoDBiEOG6G4VQG.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z5G7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRFAWRRRA SLRATSSLSL RWRVMLLAMS MVAMVVVLMA FAVYVVISAA
60 70 80 90 100
LYSDIDNQLQ SRAQLLIASG SLAADPGKAI EGTAYSDVNA MLVNPGHSIY
110 120 130 140 150
TANQPGQTLP VGTAEKAVIR GELFMSQRTA SDQRILAIHL PNDSSLLISK
160 170 180 190 200
SLRPTEAVMT KLRWVLLIVG SLGVAVAAVA GGMVTRAGLR PVGRLTEAAE
210 220 230 240 250
RVARTDDLRP IPVFGSDELA RLTEAFNLML RALAESRERQ ARLVTDAGHE
260 270 280 290 300
LRTPLTSLRT NVELLIASMA PEAPRLPDQE MADLRADVLA QIEELSTLVG
310 320 330 340 350
DLVDLTRDDA GQVVHEPIDM SEVLYRSLER VRRRRNDIHF DVQAIGWQIY
360 370 380 390 400
GDAAGLSRAV LNLMDNAAKW SPSGGRVVVT MRQFDPSHVE LVVSDYGPGI
410 420 430 440 450
PPQERRLVFE RFYRSTTARS LPGSGLGLAI VKQVVINHGG LLRVEDTAPG
460 470 480 490 500
VQPPGTSIYV LLPGRPMPVS AYSTLADQDM GEANFQDKIG PAVQVSGKSA
510
NFRDSAHVIS VDYQSARAR
Length:519
Mass (Da):56,471
Last modified:May 1, 1999 - v1
Checksum:iC0F2DA4D57364F10
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL035500 Genomic DNA. Translation: CAB36689.1.
AL583917 Genomic DNA. Translation: CAC29683.1.
PIRiT45447.
RefSeqiNP_301251.1. NC_002677.1.
WP_010907576.1. NC_002677.1.

Genome annotation databases

EnsemblBacteriaiCAC29683; CAC29683; CAC29683.
GeneIDi908554.
KEGGimle:ML0175.
PATRICi18050653. VBIMycLep78757_0280.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL035500 Genomic DNA. Translation: CAB36689.1.
AL583917 Genomic DNA. Translation: CAC29683.1.
PIRiT45447.
RefSeqiNP_301251.1. NC_002677.1.
WP_010907576.1. NC_002677.1.

3D structure databases

ProteinModelPortaliQ9Z5G7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML0175.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC29683; CAC29683; CAC29683.
GeneIDi908554.
KEGGimle:ML0175.
PATRICi18050653. VBIMycLep78757_0280.

Organism-specific databases

LepromaiML0175.

Phylogenomic databases

eggNOGiENOG41062D9. Bacteria.
COG0642. LUCA.
HOGENOMiHOG000223177.
KOiK07653.
OMAiQEMVELR.
OrthoDBiEOG6G4VQG.

Family and domain databases

Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
[Graphical view]
SUPFAMiSSF47384. SSF47384. 1 hit.
SSF55874. SSF55874. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: TN.

Entry informationi

Entry nameiMPRB_MYCLE
AccessioniPrimary (citable) accession number: Q9Z5G7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 23, 2007
Last sequence update: May 1, 1999
Last modified: November 11, 2015
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.