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Protein

Glyceraldehyde-3-phosphate dehydrogenase

Gene

gap

Organism
Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the oxidative phosphorylation of glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NAD. The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NAD to NADH. The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG.By similarity

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.By similarity

Enzyme regulationi

Resistant to pentalenolactone.

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.Curated
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA), 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI)
  4. Enolase 2 (eno2), Enolase 1 (eno1)
  5. Pyruvate kinase (SCO5423), Pyruvate kinase (SCO2014)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei35NADBy similarity1
Binding sitei79NAD; via carbonyl oxygenBy similarity1
Binding sitei121NADBy similarity1
Active sitei153NucleophileBy similarity1
Sitei180Activates thiol group during catalysisBy similarity1
Binding sitei183Glyceraldehyde 3-phosphateBy similarity1
Binding sitei184NADBy similarity1
Binding sitei198Glyceraldehyde 3-phosphateBy similarity1
Binding sitei234Glyceraldehyde 3-phosphateBy similarity1
Binding sitei317NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 13NADBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processGlycolysis
LigandNAD, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenaseBy similarity (EC:1.2.1.12By similarity)
Short name:
GAPDHBy similarity
Alternative name(s):
NAD-dependent glyceraldehyde-3-phosphate dehydrogenaseBy similarity
Gene namesi
Name:gap
Ordered Locus Names:SCO1947
ORF Names:SCC54.07c
OrganismiStreptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
Taxonomic identifieri100226 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomycesStreptomyces albidoflavus group
Proteomesi
  • UP000001973 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001457011 – 336Glyceraldehyde-3-phosphate dehydrogenaseAdd BLAST336

Proteomic databases

PRIDEiQ9Z518

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi100226.SCO1947

Structurei

3D structure databases

ProteinModelPortaliQ9Z518
SMRiQ9Z518
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni152 – 154Glyceraldehyde 3-phosphate bindingBy similarity3
Regioni211 – 212Glyceraldehyde 3-phosphate bindingBy similarity2

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C17 Bacteria
COG0057 LUCA
HOGENOMiHOG000071679
InParanoidiQ9Z518
KOiK00134
OMAiFTLENMV
OrthoDBiPOG091H02BL
PhylomeDBiQ9Z518

Family and domain databases

InterProiView protein in InterPro
IPR020831 GlycerAld/Erythrose_P_DH
IPR020829 GlycerAld_3-P_DH_cat
IPR020828 GlycerAld_3-P_DH_NAD(P)-bd
IPR006424 Glyceraldehyde-3-P_DH_1
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF02800 Gp_dh_C, 1 hit
PF00044 Gp_dh_N, 1 hit
PIRSFiPIRSF000149 GAP_DH, 1 hit
PRINTSiPR00078 G3PDHDRGNASE
SMARTiView protein in SMART
SM00846 Gp_dh_N, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR01534 GAPDH-I, 1 hit

Sequencei

Sequence statusi: Complete.

Q9Z518-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIRVGINGF GRIGRNYFRA LLEQGADIEI VAVNDLGDTA TTAHLLKYDT
60 70 80 90 100
ILGRLKAEVS HTEDTITVDG KTIKVLSERN PADIPWGELG VDIVIESTGI
110 120 130 140 150
FTKKADAEKH IAGGAKKVLI SAPAKDEDIT IVMGVNQDKY DPANHHVISN
160 170 180 190 200
ASCTTNCVAP MAKVLDENFG IVKGLMTTVH AYTNDQRILD FPHKDLRRAR
210 220 230 240 250
AAAENIIPTT TGAAKATALV LPQLKGKLDG IAMRVPVPTG SATDLVVELQ
260 270 280 290 300
REVTKDEVNA AFKKAADDGD LKGILFYTED AIVSSDITGD PASCTFDSSL
310 320 330
TMVQEGKSVK ILGWYDNEWG YSNRLVDLTV FVGNQL
Length:336
Mass (Da):36,268
Last modified:May 1, 1999 - v1
Checksum:i91C17A1EB1EE3C18
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL939110 Genomic DNA Translation: CAB38137.1
PIRiT36020
RefSeqiNP_626211.1, NC_003888.3
WP_003976871.1, NC_003888.3

Genome annotation databases

EnsemblBacteriaiCAB38137; CAB38137; CAB38137
GeneIDi1097381
KEGGisco:SCO1947
PATRICifig|100226.15.peg.1974

Similar proteinsi

Entry informationi

Entry nameiG3P_STRCO
AccessioniPrimary (citable) accession number: Q9Z518
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: May 1, 1999
Last modified: May 23, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health