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Protein

Quinoprotein ethanol dehydrogenase

Gene

exaA

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of primary alcohols except for methanol that is a very poor substrate.

Catalytic activityi

A primary alcohol + 2 cytochrome c = an aldehyde + 2 reduced cytochrome c + 2 H+.

Cofactori

Protein has several cofactor binding sites:
  • pyrroloquinoline quinoneNote: Binds 1 PQQ group per subunit. PQQ is inserted between disulfide Cys-139-Cys-140 and the plane of Trp-282.
  • Ca2+Note: Binds 2 calcium ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi45Calcium 21
Metal bindingi48Calcium 21
Metal bindingi51Calcium 21
Metal bindingi213Calcium 11
Metal bindingi300Calcium 11
Active sitei350Proton acceptorSequence analysis1
Metal bindingi350Calcium 11

GO - Molecular functioni

GO - Biological processi

  • ethanol oxidation Source: PseudoCAP
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

Calcium, Metal-binding, PQQ

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15517.
BRENDAi1.1.2.8. 5087.
1.1.2.B3. 5087.

Names & Taxonomyi

Protein namesi
Recommended name:
Quinoprotein ethanol dehydrogenase (EC:1.1.2.8)
Short name:
QEDH
Gene namesi
Name:exaA
Ordered Locus Names:PA1982
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA1982.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 34Add BLAST34
ChainiPRO_000002556535 – 623Quinoprotein ethanol dehydrogenaseAdd BLAST589

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi139 ↔ 140

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiQ9Z4J7.
PRIDEiQ9Z4J7.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi208964.PA1982.

Structurei

Secondary structure

1623
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi39 – 43Combined sources5
Helixi45 – 47Combined sources3
Turni72 – 74Combined sources3
Helixi75 – 77Combined sources3
Beta strandi79 – 85Combined sources7
Beta strandi99 – 101Combined sources3
Beta strandi104 – 109Combined sources6
Turni110 – 112Combined sources3
Beta strandi113 – 121Combined sources9
Beta strandi124 – 129Combined sources6
Beta strandi148 – 150Combined sources3
Beta strandi153 – 158Combined sources6
Turni159 – 161Combined sources3
Beta strandi162 – 170Combined sources9
Beta strandi173 – 178Combined sources6
Helixi182 – 184Combined sources3
Beta strandi193 – 196Combined sources4
Turni198 – 200Combined sources3
Beta strandi203 – 207Combined sources5
Helixi212 – 214Combined sources3
Beta strandi219 – 223Combined sources5
Turni225 – 227Combined sources3
Beta strandi230 – 237Combined sources8
Beta strandi241 – 244Combined sources4
Beta strandi247 – 253Combined sources7
Helixi272 – 276Combined sources5
Beta strandi286 – 288Combined sources3
Turni289 – 292Combined sources4
Beta strandi293 – 298Combined sources6
Beta strandi301 – 304Combined sources4
Helixi306 – 309Combined sources4
Beta strandi325 – 330Combined sources6
Turni332 – 334Combined sources3
Beta strandi337 – 344Combined sources8
Beta strandi358 – 363Combined sources6
Beta strandi365 – 367Combined sources3
Beta strandi369 – 376Combined sources8
Beta strandi380 – 386Combined sources7
Turni387 – 389Combined sources3
Beta strandi392 – 400Combined sources9
Beta strandi404 – 408Combined sources5
Turni410 – 412Combined sources3
Beta strandi415 – 417Combined sources3
Beta strandi436 – 440Combined sources5
Turni455 – 457Combined sources3
Beta strandi460 – 465Combined sources6
Beta strandi467 – 473Combined sources7
Beta strandi487 – 494Combined sources8
Beta strandi499 – 505Combined sources7
Turni507 – 509Combined sources3
Beta strandi512 – 520Combined sources9
Beta strandi527 – 529Combined sources3
Turni530 – 532Combined sources3
Beta strandi533 – 537Combined sources5
Beta strandi541 – 547Combined sources7
Turni548 – 550Combined sources3
Beta strandi553 – 558Combined sources6
Beta strandi568 – 572Combined sources5
Beta strandi575 – 582Combined sources8
Helixi587 – 591Combined sources5
Helixi594 – 599Combined sources6
Beta strandi607 – 612Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FLGX-ray2.60A/B35-616[»]
ProteinModelPortaliQ9Z4J7.
SMRiQ9Z4J7.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Z4J7.

Family & Domainsi

Sequence similaritiesi

Belongs to the bacterial PQQ dehydrogenase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105DZG. Bacteria.
COG4993. LUCA.
HOGENOMiHOG000217981.
InParanoidiQ9Z4J7.
KOiK00114.
OMAiKLTPAWS.
PhylomeDBiQ9Z4J7.

Family and domain databases

Gene3Di2.140.10.10. 1 hit.
InterProiIPR018391. PQQ_beta_propeller_repeat.
IPR017512. PQQ_MeOH/EtOH_DH.
IPR002372. PQQ_repeat.
IPR027295. Quinoprotein_ADH-like_fam.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR001479. Quinoprotein_DH_CS.
[Graphical view]
PfamiPF01011. PQQ. 1 hit.
PF13360. PQQ_2. 1 hit.
[Graphical view]
SMARTiSM00564. PQQ. 6 hits.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 1 hit.
TIGRFAMsiTIGR03075. PQQ_enz_alc_DH. 1 hit.
PROSITEiPS00363. BACTERIAL_PQQ_1. 1 hit.
PS00364. BACTERIAL_PQQ_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z4J7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTRTSPAPA GLLRPSLHCL AFAVALGSAG AALAKDVTWE DIANDDKTTG
60 70 80 90 100
DVLQYGMGTH AQRWSPLKQV NADNVFKLTP AWSYSFGDEK QRGQESQAIV
110 120 130 140 150
SDGVIYVTAS YSRLFALDAK TGKRLWTYNH RLPDDIRPCC DVVNRGAAIY
160 170 180 190 200
GDKVFFGTLD ASVVALNKNT GKVVWKKKFA DHGAGYTMTG APTIVKDGKT
210 220 230 240 250
GKVLLIHGSS GDEFGVVGRL FARDPDTGEE IWMRPFVEGH MGRLNGKDST
260 270 280 290 300
VTGDVKAPSW PDDRNSPTGK VESWSHGGGA PWQSASFDAE TNTIIVGAGN
310 320 330 340 350
PGPWNTWART AKGGNPHDYD SLYTSGQVGV DPSSGEVKWF YQHTPNDAWD
360 370 380 390 400
FSGNNELVLF DYKAKDGKIV KATAHADRNG FFYVVDRSNG KLQNAFPFVD
410 420 430 440 450
NITWASHIDL KTGRPVEREG QRPPLPEPGQ KHGKAVEVSP PFLGGKNWNP
460 470 480 490 500
MAYSQDTGLF YVPANHWKED YWTEEVSYTK GSAYLGMGFR IKRMYDDHVG
510 520 530 540 550
SLRAMDPVSG KVVWEHKEHL PLWAGVLATA GNLVFTGTGD GYFKAFDAKS
560 570 580 590 600
GKELWKFQTG SGIVSPPITW EQDGEQYLGV TVGYGGAVPL WGGDMADLTR
610 620
PVAQGGSFWV FKLPSWDNRT ASR
Length:623
Mass (Da):68,123
Last modified:May 1, 1999 - v1
Checksum:i32DDE5DF20B291D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ009858 Genomic DNA. Translation: CAA08896.1.
AE004091 Genomic DNA. Translation: AAG05370.1.
AF068264 Genomic DNA. Translation: AAC79657.1.
PIRiB83399.
RefSeqiNP_250672.1. NC_002516.2.
WP_003088524.1. NZ_ASJY01000280.1.

Genome annotation databases

EnsemblBacteriaiAAG05370; AAG05370; PA1982.
GeneIDi880475.
KEGGipae:PA1982.
PATRICi19838371. VBIPseAer58763_2066.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ009858 Genomic DNA. Translation: CAA08896.1.
AE004091 Genomic DNA. Translation: AAG05370.1.
AF068264 Genomic DNA. Translation: AAC79657.1.
PIRiB83399.
RefSeqiNP_250672.1. NC_002516.2.
WP_003088524.1. NZ_ASJY01000280.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1FLGX-ray2.60A/B35-616[»]
ProteinModelPortaliQ9Z4J7.
SMRiQ9Z4J7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA1982.

Proteomic databases

PaxDbiQ9Z4J7.
PRIDEiQ9Z4J7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG05370; AAG05370; PA1982.
GeneIDi880475.
KEGGipae:PA1982.
PATRICi19838371. VBIPseAer58763_2066.

Organism-specific databases

PseudoCAPiPA1982.

Phylogenomic databases

eggNOGiENOG4105DZG. Bacteria.
COG4993. LUCA.
HOGENOMiHOG000217981.
InParanoidiQ9Z4J7.
KOiK00114.
OMAiKLTPAWS.
PhylomeDBiQ9Z4J7.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15517.
BRENDAi1.1.2.8. 5087.
1.1.2.B3. 5087.

Miscellaneous databases

EvolutionaryTraceiQ9Z4J7.

Family and domain databases

Gene3Di2.140.10.10. 1 hit.
InterProiIPR018391. PQQ_beta_propeller_repeat.
IPR017512. PQQ_MeOH/EtOH_DH.
IPR002372. PQQ_repeat.
IPR027295. Quinoprotein_ADH-like_fam.
IPR011047. Quinoprotein_ADH-like_supfam.
IPR001479. Quinoprotein_DH_CS.
[Graphical view]
PfamiPF01011. PQQ. 1 hit.
PF13360. PQQ_2. 1 hit.
[Graphical view]
SMARTiSM00564. PQQ. 6 hits.
[Graphical view]
SUPFAMiSSF50998. SSF50998. 1 hit.
TIGRFAMsiTIGR03075. PQQ_enz_alc_DH. 1 hit.
PROSITEiPS00363. BACTERIAL_PQQ_1. 1 hit.
PS00364. BACTERIAL_PQQ_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEXAA_PSEAE
AccessioniPrimary (citable) accession number: Q9Z4J7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: November 2, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.