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Reviewed, UniProtKB/Swiss-Prot Q9Z4J7 (EXAA_PSEAE)

Last modified June 16, 2009. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Quinoprotein ethanol dehydrogenase
      Short name=QEDH
    EC=1.1.99.-
Gene names
Name: exaA
Ordered Locus Names: PA1982
OrganismPseudomonas aeruginosa [Complete proteome] [HAMAP]
Taxonomic identifier287 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

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Protein attributes

Sequence length623 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Oxidizes primary alcohols and also acts on secondary alcohol, but not highly active on methanol.

Cofactor

Binds 1 PQQ group per subunit. PQQ is inserted between disulfide Cys-139-Cys-140 and the plane of Trp-282.

Binds 2 calcium ions per subunit.

Subunit structure

Homodimer.

Subcellular location

Periplasm.

Sequence similarities

Belongs to the bacterial PQQ dehydrogenase family.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3434
Chain35 – 623589Quinoprotein ethanol dehydrogenase
PRO_0000025565

Sites

Active site3501Proton acceptor Potential
Metal binding451Calcium 2
Metal binding481Calcium 2
Metal binding511Calcium 2
Metal binding2131Calcium 1
Metal binding3001Calcium 1
Metal binding3501Calcium 1

Amino acid modifications

Disulfide bond139 ↔ 140

Secondary structure

1 .................................................................................................................. 623
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
Q9Z4J7-1 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 32DDE5DF20B291D6

FASTA62368,123
        10         20         30         40         50         60 
MTTRTSPAPA GLLRPSLHCL AFAVALGSAG AALAKDVTWE DIANDDKTTG DVLQYGMGTH 

        70         80         90        100        110        120 
AQRWSPLKQV NADNVFKLTP AWSYSFGDEK QRGQESQAIV SDGVIYVTAS YSRLFALDAK 

       130        140        150        160        170        180 
TGKRLWTYNH RLPDDIRPCC DVVNRGAAIY GDKVFFGTLD ASVVALNKNT GKVVWKKKFA 

       190        200        210        220        230        240 
DHGAGYTMTG APTIVKDGKT GKVLLIHGSS GDEFGVVGRL FARDPDTGEE IWMRPFVEGH 

       250        260        270        280        290        300 
MGRLNGKDST VTGDVKAPSW PDDRNSPTGK VESWSHGGGA PWQSASFDAE TNTIIVGAGN 

       310        320        330        340        350        360 
PGPWNTWART AKGGNPHDYD SLYTSGQVGV DPSSGEVKWF YQHTPNDAWD FSGNNELVLF 

       370        380        390        400        410        420 
DYKAKDGKIV KATAHADRNG FFYVVDRSNG KLQNAFPFVD NITWASHIDL KTGRPVEREG 

       430        440        450        460        470        480 
QRPPLPEPGQ KHGKAVEVSP PFLGGKNWNP MAYSQDTGLF YVPANHWKED YWTEEVSYTK 

       490        500        510        520        530        540 
GSAYLGMGFR IKRMYDDHVG SLRAMDPVSG KVVWEHKEHL PLWAGVLATA GNLVFTGTGD 

       550        560        570        580        590        600 
GYFKAFDAKS GKELWKFQTG SGIVSPPITW EQDGEQYLGV TVGYGGAVPL WGGDMADLTR 

       610        620 
PVAQGGSFWV FKLPSWDNRT ASR

« Hide

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References

« Hide 'large scale' references
[1]"Quinoprotein ethanol dehydrogenase of Pseudomonas aeruginosa is a homodimer: sequence of the gene and deduced structural properties of the enzyme."
Diehl A., Wintzingerode F., Goerisch H.
Eur. J. Biochem. 257:409-419(1998) [PubMed: 9826187] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 17933.
[2]"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen."
Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. expand/collapse author list , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.
Nature 406:959-964(2000) [PubMed: 10984043] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[3]"Cytochrome c550 is an essential component of the quinoprotein ethanol oxidation system in Pseudomonas aeruginosa: cloning and sequencing of the genes encoding cytochrome c550 and an adjacent acetaldehyde dehydrogenase."
Schobert M., Goerisch H.
Microbiology 145:471-481(1999) [PubMed: 10075429] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-52.
Strain: ATCC 17933.
[4]"X-ray structure of the quinoprotein ethanol dehydrogenase from Pseudomonas aeruginosa: basis of substrate specificity."
Keitel T., Diehl A., Knaute T., Stezowski J.J., Hoehne W., Goerisch H.
J. Mol. Biol. 297:961-974(2000) [PubMed: 10736230] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 35-616.
Strain: ATCC 17933.

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Cross-references

Sequence databases

AJ009858 Genomic DNA. Translation: CAA08896.1.
AE004091 Genomic DNA. Translation: AAG05370.1.
AF068264 Genomic DNA. Translation: AAC79657.1.
PIRB83399.
RefSeqNP_250672.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1FLGX-ray2.60A/B35-616[»]
ModBaseSearch...

Genome annotation databases

GeneID880475.
GenomeReviewsGene locus PA1982 in contig AE004091_GR.
KEGGpae:PA1982.
NMPDRfig|208964.1.peg.1982.

Organism-specific databases

PseudoCAPPA1982.
CMRSearch...

Phylogenomic databases

HOGENOMQ9Z4J7.
OMAQ9Z4J7. VEGHMGR.

Enzyme and pathway databases

BioCycPAER208964:PA1982-MON.

Family and domain databases

InterProIPR019556. PQQ-dependent_C.
IPR019551. PQQ-dependent_N.
IPR018391. PQQ_beta_propeller_repeat.
IPR017512. PQQ_MeOH/EtOH_DH.
IPR002372. PQQ_repeat.
IPR011047. Quino_AlcDH-like.
IPR001479. Quinoprotein_DH_CS.
[Graphical view]
Gene3DG3DSA:2.140.10.10. Quinoprotein_alc_DH-like. 1 hit.
PfamPF01011. PQQ. 3 hits.
PF10535. PQQ_C. 1 hit.
PF10527. PQQ_N. 1 hit.
[Graphical view]
SMARTSM00564. PQQ. 6 hits.
[Graphical view]
TIGRFAMsTIGR03075. PQQ_enz_alc_DH. 1 hit.
PROSITEPS00363. BACTERIAL_PQQ_1. 1 hit.
PS00364. BACTERIAL_PQQ_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameEXAA_PSEAE
AccessionPrimary (citable) accession number: Q9Z4J7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: June 16, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents