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Protein

Glucose-6-phosphate 1-dehydrogenase

Gene

zwf

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the oxidation of glucose 6-phosphate to 6-phosphogluconolactone.UniRule annotation

Catalytic activityi

D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH.UniRule annotation

Pathway:ipentose phosphate pathway

This protein is involved in step 1 of the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage).UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Glucose-6-phosphate 1-dehydrogenase (zwf)
  2. 6-phosphogluconolactonase (pgl)
  3. 6-phosphogluconate dehydrogenase, decarboxylating (gnd)
This subpathway is part of the pathway pentose phosphate pathway, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribulose 5-phosphate from D-glucose 6-phosphate (oxidative stage), the pathway pentose phosphate pathway and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei51 – 511NADPUniRule annotation
Binding sitei150 – 1501NADPUniRule annotation
Binding sitei180 – 1801SubstrateUniRule annotation
Binding sitei184 – 1841SubstrateUniRule annotation
Binding sitei218 – 2181SubstrateUniRule annotation
Binding sitei237 – 2371SubstrateUniRule annotation
Active sitei242 – 2421Proton acceptorUniRule annotation
Binding sitei341 – 3411SubstrateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciSMEL266834:GJF6-721-MONOMER.
UniPathwayiUPA00115; UER00408.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucose-6-phosphate 1-dehydrogenaseUniRule annotation (EC:1.1.1.49UniRule annotation)
Short name:
G6PDUniRule annotation
Gene namesi
Name:zwfUniRule annotation
Ordered Locus Names:R00704
ORF Names:SMc03070
OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri266834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
ProteomesiUP000001976 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 491491Glucose-6-phosphate 1-dehydrogenasePRO_0000068132Add
BLAST

Proteomic databases

PRIDEiQ9Z3S2.

Interactioni

Protein-protein interaction databases

STRINGi266834.SMc03070.

Structurei

3D structure databases

ProteinModelPortaliQ9Z3S2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glucose-6-phosphate dehydrogenase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0364.
HOGENOMiHOG000046191.
KOiK00036.
OMAiVRDVTMD.
OrthoDBiEOG61308Z.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD.
InterProiIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z3S2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSQIIPVEP FDYVVFGGTG DLAERKLLPA LYHRQMEGQF TEPTRIIGAS
60 70 80 90 100
RASLSHDEYR RFASDALKEH LKSGEFNEAE VEKFTSRLYY VSVDAKSEQG
110 120 130 140 150
WDDLKKLLEE GKDRTRAFYL AVGPAIFSDI SEKIRDHKLI TRNTRIVVEK
160 170 180 190 200
PIGRDLASAT ELNDTIGKVF REEQIFRIDH YLGKETVQNL MALRFANALY
210 220 230 240 250
EPLWNSAHID HVQITVSEAV GLENRAGYYD KAGALRDMVQ NHILQLLCFV
260 270 280 290 300
AMEAPTSMDA EAVRDEKLKV LRALKPITAS NVEQVTVRGQ YRAGASSGGP
310 320 330 340 350
VKGYLEELEG GVSNTETFVA IKAEISNWRW AGVPFYLRTG KRMAGRMSEI
360 370 380 390 400
VITFKQIPHS IFDQSAGRIS ANQLMIRLQP NEGVKQSLMI KDPGPGGMRL
410 420 430 440 450
RNVPLDMSFA EAFAVRNADA YERLLLDVIR NNQTLFVRRD EVEAAWQWID
460 470 480 490
PILKAWEATG QQVQGYTAGT WGPSQSIALI ERDGRTWNDA I
Length:491
Mass (Da):55,302
Last modified:November 2, 2001 - v2
Checksum:i0D8B1AFD094E1775
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti401 – 4011R → T in AAD12043 (PubMed:10400573).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF045609 Genomic DNA. Translation: AAD12043.1.
AL591688 Genomic DNA. Translation: CAC45276.1.
RefSeqiNP_384810.1. NC_003047.1.
WP_003527054.1. NC_003047.1.

Genome annotation databases

EnsemblBacteriaiCAC45276; CAC45276; SMc03070.
GeneIDi1232343.
KEGGisme:SMc03070.
PATRICi23630629. VBISinMel96828_2080.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF045609 Genomic DNA. Translation: AAD12043.1.
AL591688 Genomic DNA. Translation: CAC45276.1.
RefSeqiNP_384810.1. NC_003047.1.
WP_003527054.1. NC_003047.1.

3D structure databases

ProteinModelPortaliQ9Z3S2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266834.SMc03070.

Proteomic databases

PRIDEiQ9Z3S2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC45276; CAC45276; SMc03070.
GeneIDi1232343.
KEGGisme:SMc03070.
PATRICi23630629. VBISinMel96828_2080.

Phylogenomic databases

eggNOGiCOG0364.
HOGENOMiHOG000046191.
KOiK00036.
OMAiVRDVTMD.
OrthoDBiEOG61308Z.

Enzyme and pathway databases

UniPathwayiUPA00115; UER00408.
BioCyciSMEL266834:GJF6-721-MONOMER.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
HAMAPiMF_00966. G6PD.
InterProiIPR001282. G6P_DH.
IPR019796. G6P_DH_AS.
IPR022675. G6P_DH_C.
IPR022674. G6P_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR23429. PTHR23429. 1 hit.
PfamiPF02781. G6PD_C. 1 hit.
PF00479. G6PD_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000110. G6PD. 1 hit.
PRINTSiPR00079. G6PDHDRGNASE.
TIGRFAMsiTIGR00871. zwf. 1 hit.
PROSITEiPS00069. G6P_DEHYDROGENASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel Sinorhizobium meliloti operon encodes an alpha-glucosidase and a periplasmic-binding-protein-dependent transport system for alpha-glucosides."
    Willis L.B., Walker G.C.
    J. Bacteriol. 181:4176-4184(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1021.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1021.

Entry informationi

Entry nameiG6PD_RHIME
AccessioniPrimary (citable) accession number: Q9Z3S2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 2, 2001
Last modified: July 22, 2015
This is version 99 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.