Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q9Z3R8 (AGLA_RHIME) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable alpha-glucosidase

EC=3.2.1.20
Gene names
Name:aglA
Ordered Locus Names:R00698
ORF Names:SMc03064
OrganismRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti) [Complete proteome] [HAMAP]
Taxonomic identifier266834 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium

Protein attributes

Sequence length551 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.

Sequence similarities

Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functioncation binding

Inferred from electronic annotation. Source: InterPro

maltose alpha-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 551551Probable alpha-glucosidase
PRO_0000054312

Sites

Active site2121Nucleophile By similarity
Active site2721Proton donor By similarity
Site3451Transition state stabilizer By similarity

Experimental info

Sequence conflict131P → A in AAD12047. Ref.1
Sequence conflict20 – 212GA → RP in AAD12047. Ref.1
Sequence conflict402 – 41514YGIQF…KGRDG → MASSSGPTSSAGR Ref.1
Sequence conflict445 – 46016PRAVA…ASVLH → RGRCRAGGRPGLGAA in AAD12047. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9Z3R8 [UniParc].

Last modified November 2, 2001. Version 2.
Checksum: BB7BD3E17C935509

FASTA55162,576
        10         20         30         40         50         60 
MTMNETTSSL LEPDRDWWRG AVIYQIYPRS FQDTNGDGIG DLQGITARLP HIAGLGADAI 

        70         80         90        100        110        120 
WISPFFTSPM RDFGYDVSNY VDVDPIFGTL EDFDALIAEA HRLGLRVMID LVLSHTSDRH 

       130        140        150        160        170        180 
PWFVESRSSR SNAKADWYVW ADSKPDGTPP NNWLSIFGGS AWQWDPTRLQ YYLHNFLTSQ 

       190        200        210        220        230        240 
PDLNLHNPQV QEALLAVERF WLERGVDGFR LDTINFYFHD RELRDNPALV PERRNASTAP 

       250        260        270        280        290        300 
AVNPYNYQEH IYDKNRPENL EFLKRFRAVM DEFPAIAAVG EVGDSQRGLE IAGEYTSGGD 

       310        320        330        340        350        360 
KVHMCYAFEF LAPDRLTPQR VAEVLRDFHR AAPEGWACWA FSNHDVVRHV SRWADGVTDH 

       370        380        390        400        410        420 
DAHAKLLASL LMSLRGTVCI YQGEELALAE AELDYEDLQD PYGIQFWPDF KGRDGCRTPM 

       430        440        450        460        470        480 
VWESLPDGGF SSATPWLPIS QSHIPRAVAV QEGDPASVLH HYRRFLAFRK ANPALAKGEI 

       490        500        510        520        530        540 
EFVETRGSLL GFLRSHGNEK VFCLFNMSDE AATKELPMKR LEPLEGHGFV SEILDHEVKL 

       550 
PAWGAFFARL A 

« Hide

References

« Hide 'large scale' references
[1]"A novel Sinorhizobium meliloti operon encodes an alpha-glucosidase and a periplasmic-binding-protein-dependent transport system for alpha-glucosides."
Willis L.B., Walker G.C.
J. Bacteriol. 181:4176-4184(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021."
Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. expand/collapse author list , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F.
Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1021.
[3]"The composite genome of the legume symbiont Sinorhizobium meliloti."
Galibert F., Finan T.M., Long S.R., Puehler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M.J., Becker A., Boistard P., Bothe G., Boutry M., Bowser L., Buhrmester J., Cadieu E., Capela D., Chain P., Cowie A. expand/collapse author list , Davis R.W., Dreano S., Federspiel N.A., Fisher R.F., Gloux S., Godrie T., Goffeau A., Golding B., Gouzy J., Gurjal M., Hernandez-Lucas I., Hong A., Huizar L., Hyman R.W., Jones T., Kahn D., Kahn M.L., Kalman S., Keating D.H., Kiss E., Komp C., Lelaure V., Masuy D., Palm C., Peck M.C., Pohl T.M., Portetelle D., Purnelle B., Ramsperger U., Surzycki R., Thebault P., Vandenbol M., Vorhoelter F.J., Weidner S., Wells D.H., Wong K., Yeh K.-C., Batut J.
Science 293:668-672(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1021.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF045609 Genomic DNA. Translation: AAD12047.1.
AL591688 Genomic DNA. Translation: CAC45270.1.
RefSeqNP_384804.1. NC_003047.1.

3D structure databases

ProteinModelPortalQ9Z3R8.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING266834.SMc03064.

Protein family/group databases

CAZyGH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAC45270; CAC45270; SMc03064.
GeneID1232337.
KEGGsme:SMc03064.
PATRIC23630615. VBISinMel96828_2073.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0366.
HOGENOMHOG000220640.
KOK01187.
OMATREYGNE.
OrthoDBEOG6RVFV2.

Enzyme and pathway databases

BioCycSMEL266834:GJF6-715-MONOMER.

Family and domain databases

Gene3D2.60.40.1180. 1 hit.
3.20.20.80. 2 hits.
InterProIPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR006589. Glyco_hydro_13_sub_cat_dom.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PANTHERPTHR10357. PTHR10357. 1 hit.
PfamPF00128. Alpha-amylase. 1 hit.
[Graphical view]
SMARTSM00642. Aamy. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
ProtoNetSearch...

Entry information

Entry nameAGLA_RHIME
AccessionPrimary (citable) accession number: Q9Z3R8
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: November 2, 2001
Last modified: May 14, 2014
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries