Reviewed, UniProtKB/Swiss-Prot Q9Z3R8 (AGLA_RHIME)

Names and origin

Protein names

Recommended name:
    Probable alpha-glucosidase
    EC=3.2.1.20

Gene names

Name:	aglA
Ordered Locus Names:	R00698
ORF Names:	SMc03064

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	551 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Molecular function	Glycosidase Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	alpha-glucosidase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 551	551	Probable alpha-glucosidase		PRO_0000054312
Sites
	Active site	212	1	Nucleophile By similarity
	Active site	272	1	Proton donor By similarity
	Active site	345	1	By similarity
Experimental info
	Sequence conflict	13	1	P → A in AAD12047. Ref.1
	Sequence conflict	20 – 21	2	GA → RP in AAD12047. Ref.1
	Sequence conflict	402 – 415	14	YGIQF…KGRDG → MASSSGPTSSAGR Ref.1
	Sequence conflict	445 – 460	16	PRAVA…ASVLH → RGRCRAGGRPGLGAA in AAD12047. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9Z3R8-1 [UniParc]. Last modified November 2, 2001. Version 2. Checksum: BB7BD3E17C935509	FASTA	551	62,576
	10 20 30 40 50 60 MTMNETTSSL LEPDRDWWRG AVIYQIYPRS FQDTNGDGIG DLQGITARLP HIAGLGADAI 70 80 90 100 110 120 WISPFFTSPM RDFGYDVSNY VDVDPIFGTL EDFDALIAEA HRLGLRVMID LVLSHTSDRH 130 140 150 160 170 180 PWFVESRSSR SNAKADWYVW ADSKPDGTPP NNWLSIFGGS AWQWDPTRLQ YYLHNFLTSQ 190 200 210 220 230 240 PDLNLHNPQV QEALLAVERF WLERGVDGFR LDTINFYFHD RELRDNPALV PERRNASTAP 250 260 270 280 290 300 AVNPYNYQEH IYDKNRPENL EFLKRFRAVM DEFPAIAAVG EVGDSQRGLE IAGEYTSGGD 310 320 330 340 350 360 KVHMCYAFEF LAPDRLTPQR VAEVLRDFHR AAPEGWACWA FSNHDVVRHV SRWADGVTDH 370 380 390 400 410 420 DAHAKLLASL LMSLRGTVCI YQGEELALAE AELDYEDLQD PYGIQFWPDF KGRDGCRTPM 430 440 450 460 470 480 VWESLPDGGF SSATPWLPIS QSHIPRAVAV QEGDPASVLH HYRRFLAFRK ANPALAKGEI 490 500 510 520 530 540 EFVETRGSLL GFLRSHGNEK VFCLFNMSDE AATKELPMKR LEPLEGHGFV SEILDHEVKL 550 PAWGAFFARL A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel Sinorhizobium meliloti operon encodes an alpha-glucosidase and a periplasmic-binding-protein-dependent transport system for alpha-glucosides." Willis L.B., Walker G.C. J. Bacteriol. 181:4176-4184(1999) [PubMed: 10400573] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF045609 Genomic DNA. Translation: AAD12047.1. AL591688 Genomic DNA. Translation: CAC45270.1.
RefSeq	NP_384804.1.
3D structure databases
HSSP	HSSP built from PDB template 1UOK based on UniProtKB P21332.
ModBase	Search...
Protein family/group databases
CAZy	GH13. Glycoside Hydrolase Family 13.
Genome annotation databases
GeneID	1232337.
GenomeReviews	Gene locus R00698 in contig AL591688_GR.
KEGG	sme:SMc03064.
NMPDR	fig|266834.1.peg.1992.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9Z3R8.
OMA	SQRGLEI.
Enzyme and pathway databases
BioCyc	SMEL266834:SMC03064-MON.
BRENDA	3.2.1.20. 142.
Family and domain databases
InterPro	IPR006047. Glyco_hydro_13_cat. IPR006589. Glyco_hydro_13_sub_cat. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. [Graphical view]
SMART	SM00642. Aamy. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGLA_RHIME

Accession

Primary (citable) accession number: Q9Z3R8

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	November 2, 2001
Last modified:	November 3, 2009
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)