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Protein

Acetoacetyl-coenzyme A synthetase

Gene

acsA2

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of acetoacetate into acetoacetyl-CoA. Is involved in poly-3-hydroxybutyrate (PHB) degradation, which allows growth of R.meliloti on PHB cycle intermediates.1 Publication

Catalytic activityi

ATP + acetoacetate + CoA = AMP + diphosphate + acetoacetyl-CoA.1 Publication

Cofactori

Mg2+By similarity

Pathwayi: poly-(R)-3-hydroxybutanoate degradation

This protein is involved in the pathway poly-(R)-3-hydroxybutanoate degradation, which is part of Biopolymer metabolism.1 Publication
View all proteins of this organism that are known to be involved in the pathway poly-(R)-3-hydroxybutanoate degradation and in Biopolymer metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei504ATPBy similarity1
Binding sitei519ATPBy similarity1
Binding sitei530ATPBy similarity1
Metal bindingi546Magnesium; via carbonyl oxygenBy similarity1
Binding sitei587Coenzyme ABy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi392 – 394ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi6.2.1.16. 5347.
UniPathwayiUPA01062.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetoacetyl-coenzyme A synthetase1 Publication (EC:6.2.1.161 Publication)
Short name:
Acetoacetyl-CoA synthetase1 Publication
Gene namesi
Name:acsA2Imported
Synonyms:acsA1 Publication
Ordered Locus Names:R00719
ORF Names:SMc00774
OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri266834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001976 Componenti: Chromosome

Pathology & Biotechi

Disruption phenotypei

Acetyl-CoA synthetase activity is not affected in mutant cells, whereas acetoacetyl-CoA synthetase activity is drastically reduced. Moreover, after growth in YM medium, cells lacking this gene accumulate PHB to 60 to 70% of cell dry mass. They display reduced ability to proliferate during the first 30 days of incubation in growth medium lacking nutrient carbon.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002083811 – 650Acetoacetyl-coenzyme A synthetaseAdd BLAST650

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei612N6-acetyllysineBy similarity1

Post-translational modificationi

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.By similarity

Keywords - PTMi

Acetylation

Interactioni

Protein-protein interaction databases

STRINGi266834.SMc00774.

Structurei

3D structure databases

ProteinModelPortaliQ9Z3R3.
SMRiQ9Z3R3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni199 – 202Coenzyme A bindingBy similarity4

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4108IQF. Bacteria.
COG0365. LUCA.
HOGENOMiHOG000229992.
KOiK01907.
OMAiYEYVYRC.

Family and domain databases

InterProiView protein in InterPro
IPR005914. Acac_CoA_synth.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
PfamiView protein in Pfam
PF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
TIGRFAMsiTIGR01217. ac_ac_CoA_syn. 1 hit.
PROSITEiView protein in PROSITE
PS00455. AMP_BINDING. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9Z3R3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQAERPLWVP DREIVERSPM AEFIDWCGER FGRSFADYDA FHDWSVSERG
60 70 80 90 100
AFWTAVWEHC KVIGESGEKA LVDGDRMLDA RFFPEARLNF AENLLRKTGS
110 120 130 140 150
GDALIFRGED KVSYRLTWDE LRALVSRLQQ ALRAQGIGAG DRVAAMMPNM
160 170 180 190 200
PETIALMLAT ASVGAIWSSC SPDFGEQGVL DRFGQIAPKL FIVCDGYWYN
210 220 230 240 250
GKRQDVDSKV RAVAKSLGAP TVIVPYAGDS AALAPTVEGG VTLADFIAGF
260 270 280 290 300
QAGPLVFERL PFGHPLYILF SSGTTGVPKC IVHSAGGTLL QHLKEHRFHC
310 320 330 340 350
GLRDGERLFY FTTCGWMMWN WLASGLAVGA TLCLYDGSPF CPDGNVLFDY
360 370 380 390 400
AAAERFAVFG TSAKYIDAVR KGGFTPARTH DLSSLRLMTS TGSPLSPEGF
410 420 430 440 450
SFVYEGIKPD VQLASISGGT DIVSCFVLGN PLKPVWRGEI QGPGLGLAVD
460 470 480 490 500
VWNDEGKPVR GEKGELVCTR AFPSMPVMFW NDPDGAKYRA AYFDRFDNVW
510 520 530 540 550
CHGDFAEWTP HGGIVIHGRS DATLNPGGVR IGTAEIYNQV EQMDEVAEAL
560 570 580 590 600
CIGQDWEDDV RVVLFVRLAR GVELTEALTR EIKNRIRSGA SPRHVPAKII
610 620 630 640 650
AVADIPRTKS GKIVELAVRD VVHGRPVKNK EALANPEALD LFAGLEELKS
Length:650
Mass (Da):71,309
Last modified:May 30, 2000 - v2
Checksum:iAE2B7A41E6C57846
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti144A → S in AAC64548 (PubMed:10735852).Curated1
Sequence conflicti337G → C in AAC64548 (PubMed:10735852).Curated1
Sequence conflicti358V → L in AAC64548 (PubMed:10735852).Curated1
Sequence conflicti387L → V in AAC64548 (PubMed:10735852).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF080217 Genomic DNA. Translation: AAC64548.1.
AL591688 Genomic DNA. Translation: CAC45291.1.
RefSeqiNP_384825.1. NC_003047.1.
WP_010968769.1. NC_003047.1.

Genome annotation databases

EnsemblBacteriaiCAC45291; CAC45291; SMc00774.
GeneIDi1232358.
KEGGisme:SMc00774.
PATRICifig|266834.11.peg.2098.

Similar proteinsi

Entry informationi

Entry nameiAACS_RHIME
AccessioniPrimary (citable) accession number: Q9Z3R3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: June 7, 2017
This is version 96 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families