Reviewed, UniProtKB/Swiss-Prot Q9Z3R3 (ACSA1_RHIME)

Names and origin

Protein names

Recommended name:
    Acetoacetyl-coenzyme A synthetase
    EC=6.2.1.16
Alternative name(s):
    Acetoacetate--CoA ligase 1
    Acyl-activating enzyme 1

Gene names

Name:	acsA1
Ordered Locus Names:	R00719
ORF Names:	SMc00774

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	650 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in poly-3-hydroxybutyrate degradation. Activates acetoacetate to acetoacetyl-CoA. HAMAP MF_01123
Catalytic activity	ATP + acetoacetate + CoA = AMP + diphosphate + acetoacetyl-CoA. HAMAP MF_01123
Post-translational modification	Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme By similarity.
Sequence similarities	Belongs to the ATP-dependent AMP-binding enzyme family.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW acetate-CoA ligase activity Inferred from electronic annotation. Source: HAMAP acetoacetate-CoA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 650	650	Acetoacetyl-coenzyme A synthetase HAMAP MF_01123		PRO_0000208381
Sites
	Active site	521	1	By similarity
Amino acid modifications
	Modified residue	612	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	144	1	A → S in AAC64548. Ref.1
	Sequence conflict	337	1	G → C in AAC64548. Ref.1
	Sequence conflict	358	1	V → L in AAC64548. Ref.1
	Sequence conflict	387	1	L → V in AAC64548. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9Z3R3-1 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: AE2B7A41E6C57846	FASTA	650	71,309
	10 20 30 40 50 60 MQAERPLWVP DREIVERSPM AEFIDWCGER FGRSFADYDA FHDWSVSERG AFWTAVWEHC 70 80 90 100 110 120 KVIGESGEKA LVDGDRMLDA RFFPEARLNF AENLLRKTGS GDALIFRGED KVSYRLTWDE 130 140 150 160 170 180 LRALVSRLQQ ALRAQGIGAG DRVAAMMPNM PETIALMLAT ASVGAIWSSC SPDFGEQGVL 190 200 210 220 230 240 DRFGQIAPKL FIVCDGYWYN GKRQDVDSKV RAVAKSLGAP TVIVPYAGDS AALAPTVEGG 250 260 270 280 290 300 VTLADFIAGF QAGPLVFERL PFGHPLYILF SSGTTGVPKC IVHSAGGTLL QHLKEHRFHC 310 320 330 340 350 360 GLRDGERLFY FTTCGWMMWN WLASGLAVGA TLCLYDGSPF CPDGNVLFDY AAAERFAVFG 370 380 390 400 410 420 TSAKYIDAVR KGGFTPARTH DLSSLRLMTS TGSPLSPEGF SFVYEGIKPD VQLASISGGT 430 440 450 460 470 480 DIVSCFVLGN PLKPVWRGEI QGPGLGLAVD VWNDEGKPVR GEKGELVCTR AFPSMPVMFW 490 500 510 520 530 540 NDPDGAKYRA AYFDRFDNVW CHGDFAEWTP HGGIVIHGRS DATLNPGGVR IGTAEIYNQV 550 560 570 580 590 600 EQMDEVAEAL CIGQDWEDDV RVVLFVRLAR GVELTEALTR EIKNRIRSGA SPRHVPAKII 610 620 630 640 650 AVADIPRTKS GKIVELAVRD VVHGRPVKNK EALANPEALD LFAGLEELKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Requirement for the enzymes acetoacetyl coenzyme A synthetase and poly-3-hydroxybutyrate (PHB) synthase for growth of Sinorhizobium meliloti on PHB cycle intermediates." Cai G.-Q., Driscoll B.T., Charles T.C. J. Bacteriol. 182:2113-2118(2000) [PubMed: 10735852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SU47 / 1021.
[2]	"Analysis of the chromosome sequence of the legume symbiont Sinorhizobium meliloti strain 1021." Capela D., Barloy-Hubler F., Gouzy J., Bothe G., Ampe F., Batut J., Boistard P., Becker A., Boutry M., Cadieu E., Dreano S., Gloux S., Godrie T., Goffeau A., Kahn D., Kiss E., Lelaure V., Masuy D. , Pohl T., Portetelle D., Puehler A., Purnelle B., Ramsperger U., Renard C., Thebault P., Vandenbol M., Weidner S., Galibert F. Proc. Natl. Acad. Sci. U.S.A. 98:9877-9882(2001) [PubMed: 11481430] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF080217 Genomic DNA. Translation: AAC64548.1. AL591688 Genomic DNA. Translation: CAC45291.1.
RefSeq	NP_384825.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1232358.
GenomeReviews	Gene locus R00719 in contig AL591688_GR.
KEGG	sme:SMc00774.
NMPDR	fig|266834.1.peg.2013.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q9Z3R3.
OMA	WAHGDYA.
Enzyme and pathway databases
BioCyc	SMEL266834:SMC00774-MON.
Family and domain databases
HAMAP	MF_01123. Divergent sequence. [Tree]
InterPro	IPR005914. Acac_CoA_synth. IPR000873. AMP-dep_Synth/Lig. [Graphical view]
Pfam	PF00501. AMP-binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR01217. ac_ac_CoA_syn. 1 hit.
PROSITE	PS00455. AMP_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACSA1_RHIME

Accession

Primary (citable) accession number: Q9Z3R3

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	May 30, 2000
Last modified:	November 3, 2009
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)