Reviewed, UniProtKB/Swiss-Prot Q9Z3Q0 (CYA3_RHIME)

Names and origin

Protein names

Recommended name:
    Putative adenylate cyclase 3
    EC=4.6.1.1
Alternative name(s):
    ATP pyrophosphate-lyase 3
    Adenylyl cyclase 3

Gene names

Name:	cya3
Synonyms:	cyaF5
Ordered Locus Names:	RA0861
ORF Names:	SMa1583

Encoded on

Plasmid pSymA (megaplasmid 1)

Organism

Rhizobium meliloti (Sinorhizobium meliloti) [Complete proteome] [HAMAP]

Taxonomic identifier

382 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Sinorhizobium/Ensifer group › Sinorhizobium

Protein attributes

Sequence length	587 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP = 3',5'-cyclic AMP + diphosphate.
Sequence similarities	Belongs to the adenylyl cyclase class-3 family. Contains 1 guanylate cyclase domain. Contains 5 TPR repeats.

Ontologies

Keywords
Biological process	cAMP biosynthesis
Domain	Repeat TPR repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Lyase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	cAMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW intracellular signaling cascade Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenylate cyclase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 587	587	Putative adenylate cyclase 3		PRO_0000195746
Regions
	Domain	12 – 127	116	Guanylate cyclase
	Repeat	343 – 376	34	TPR 1
	Repeat	421 – 454	34	TPR 2
	Repeat	455 – 488	34	TPR 3
	Repeat	490 – 522	33	TPR 4
	Repeat	524 – 556	33	TPR 5
Experimental info
	Sequence conflict	18	1	A → T in CAB38103. Ref.1
	Sequence conflict	178	1	R → H in CAB38103. Ref.1
	Sequence conflict	183	1	P → L in CAB38103. Ref.1
	Sequence conflict	196 – 197	2	KP → S in CAB38103. Ref.1
	Sequence conflict	251	1	D → N in CAB38103. Ref.1
	Sequence conflict	553	1	N → D in CAB38103. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9Z3Q0-1 [UniParc]. Last modified November 2, 2001. Version 2. Checksum: 4630AC9816FB615D	FASTA	587	65,307
	10 20 30 40 50 60 MAKESIRRRL AAILAADAVG YSRLMERDEK STHTLLMARW KEVLEPLVGI HQGRVFKRTG 70 80 90 100 110 120 DGVLVEFGSA VNAVECAAAL QQAMAAANRD LPEDRAIVLR VGVNLGDIMV EDSDLFGDGV 130 140 150 160 170 180 NVAARIEALA DPGGVAISDG IHEYVHGRTD IDFVDSGYHE VKNIERPVHI WTWSPKDRAR 190 200 210 220 230 240 EPPNIAAEPP PQLPAKPSIA VLPFDNMSGD PEQGYFADGI TEDIITDLSK VSGLFVIARN 250 260 270 280 290 300 SSFAYKGKTP DIRKVSRELG VRYVLEGSVR RAANRIRINA QMIDGTTGGH LWAERYDRGL 310 320 330 340 350 360 EDIFAVQDEV TRTIVNALRV KLTAGEEERR ESRGKVDPEA YDLLVRSRQA ILQFNALSSM 370 380 390 400 410 420 EARRMLHRVL EIDPGMAAAH ASLSIIALTD FINQWNGATP DNLTQALGLA QEAIDTDGSE 430 440 450 460 470 480 PQGHYTLALA LSWMRRLDEA EHAAERAIEL DPNSANAYTA LGTIRDFQGR HEEALALYTR 490 500 510 520 530 540 AHRLDPQFDL SLHFQGRALL NLGRFDEAEV AFKRRLLLAP RSDMTRFYLA CLYGRTGRHE 550 560 570 580 EARGYWREVL GVNPSFSVDH LRRSLPYQDP HLMDRLVEGL REAGVSI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The eff-482 locus of Sinorhizobium meliloti CXM1-105 that influences symbiotic effectiveness consists of three genes encoding an endoglycanase, a transcriptional regulator and an adenylate cyclase." Sharypova L.A., Yurgel S.N., Keller M., Simarov B.V., Puehler A., Becker A. Mol. Gen. Genet. 261:1032-1044(1999) [PubMed: 10485295] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: CXM1-105.
[2]	"Nucleotide sequence and predicted functions of the entire Sinorhizobium meliloti pSymA megaplasmid." Barnett M.J., Fisher R.F., Jones T., Komp C., Abola A.P., Barloy-Hubler F., Bowser L., Capela D., Galibert F., Gouzy J., Gurjal M., Hong A., Huizar L., Hyman R.W., Kahn D., Kahn M.L., Kalman S., Keating D.H. , Palm C., Peck M.C., Surzycki R., Wells D.H., Yeh K.-C., Davis R.W., Federspiel N.A., Long S.R. Proc. Natl. Acad. Sci. U.S.A. 98:9883-9888(2001) [PubMed: 11481432] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1021.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ225896 Genomic DNA. Translation: CAB38103.1. AE006469 Genomic DNA. Translation: AAK65519.2. Different initiation.
PIR	E95369.
RefSeq	NP_436107.2.
3D structure databases
SMR	Q9Z3Q0. Positions 8-172, 365-558.
ModBase	Search...
Genome annotation databases
GeneID	6435284.
GenomeReviews	Gene locus RA0861 in contig AE006469_GR.
KEGG	sme:SMa1583.
NMPDR	fig|266834.1.peg.861.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG655328.
OMA	ANRIRIN.
Enzyme and pathway databases
BioCyc	SMEL266834:SMA1583-MONOMER.
BRENDA	4.6.1.1. 142.
Family and domain databases
InterPro	IPR001054. A/G_cyclase. IPR013026. TPR-contain. IPR011990. TPR-like_helical. IPR019734. TPR_repeat. [Graphical view]
Gene3D	G3DSA:3.30.70.1230. A/G_cyclase. 1 hit. G3DSA:1.25.40.10. TPR-like_helical. 1 hit.
Pfam	PF00211. Guanylate_cyc. 1 hit. [Graphical view]
SMART	SM00044. CYCc. 1 hit. SM00028. TPR. 4 hits. [Graphical view]
PROSITE	PS50125. GUANYLATE_CYCLASE_2. 1 hit. PS50005. TPR. 4 hits. PS50293. TPR_REGION. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYA3_RHIME

Accession

Primary (citable) accession number: Q9Z3Q0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 2, 2001
Last modified:	February 9, 2010
This is version 76 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)