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Protein

Urease subunit alpha

Gene

ureC

Organism
Actinomyces naeslundii
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.UniRule annotationSAAS annotation

Cofactori

Protein has several cofactor binding sites:
  • Ni cationUniRule annotationNote: Binds 2 nickel ions per subunit.UniRule annotation
  • Ni2+UniRule annotationNote: Binds 2 nickel ions per subunit.UniRule annotation

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).UniRule annotationSAAS annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Urease subunit alpha (ureC), Urease subunit beta (ureB), Urease subunit gamma (ureA)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi137 – 1371Nickel 1; via tele nitrogenUniRule annotation
Metal bindingi137 – 1371Nickel 2UniRule annotation
Metal bindingi139 – 1391Nickel 1; via tele nitrogenUniRule annotation
Metal bindingi139 – 1391Nickel 2UniRule annotation
Metal bindingi220 – 2201Nickel 1; via carbamate groupUniRule annotation
Metal bindingi220 – 2201Nickel 2; via carbamate groupUniRule annotation
Binding sitei222 – 2221SubstrateUniRule annotation
Metal bindingi249 – 2491Nickel 1UniRule annotation
Metal bindingi249 – 2491Nickel 2; via pros nitrogenUniRule annotation
Metal bindingi275 – 2751Nickel 1UniRule annotation
Metal bindingi275 – 2751Nickel 2; via tele nitrogenUniRule annotation
Active sitei323 – 3231Proton donorUniRule annotation
Metal bindingi363 – 3631Nickel 1UniRule annotation
Metal bindingi363 – 3631Nickel 2UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

HydrolaseUniRule annotationSAAS annotation

Keywords - Ligandi

Metal-bindingUniRule annotationSAAS annotation, NickelUniRule annotationSAAS annotation

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.

Protein family/group databases

MEROPSiM38.982.

Names & Taxonomyi

Protein namesi
Recommended name:
Urease subunit alphaUniRule annotation (EC:3.5.1.5UniRule annotationSAAS annotation)
Alternative name(s):
Urea amidohydrolase subunit alphaUniRule annotation
Gene namesi
Name:ureCUniRule annotationImported
OrganismiActinomyces naeslundiiImported
Taxonomic identifieri1655 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinomycetalesActinomycetaceaeActinomyces

Subcellular locationi

  • Cytoplasm UniRule annotationSAAS annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotationSAAS annotation

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei220 – 2201N6-carboxylysineUniRule annotation

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two nickel ions.UniRule annotation

Interactioni

Subunit structurei

Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.UniRule annotation

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini132 – 570439UreaseInterPro annotationAdd
BLAST

Sequence similaritiesi

Belongs to the urease family.UniRule annotationSAAS annotation
Contains 1 urease domain.UniRule annotation

Family and domain databases

Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z369-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKELSRREH AALYGPTTGD AVRLADTGLF AQIERDLTHR GDEAVFGGGK
60 70 80 90 100
VIRDGMGHNG QRTRDEDIPD TVITNAIIID HTGVYKADVA IRDGVISAIG
110 120 130 140 150
AAGNPDIMDD VDIVIGTSTE VIAGEHRILT AGGIDSHIHF ISPTQVATAL
160 170 180 190 200
ASGVTTMIGG GTGPSDGTNA TTITPGAWNL ARMLQAVEDF PMNIGLLGKG
210 220 230 240 250
HASAREPLAE QLRAGAVGFK IHEDWGATHA VIDEALKVAD EFDVQVAIHT
260 270 280 290 300
DTLNECGFVE DTRRAIGGRV IHTFHTEGAG GGHAPDIITL AQDSNILPSS
310 320 330 340 350
TNPTLPFTRN TAEEHLDMLM VCHHLNPAIP EDVAFADSRI RPETIAAEDV
360 370 380 390 400
LHDLGVFSMT SSDSQAMGRV GEVIIRTWQV ADQMKKARGR LAGDPEDGDN
410 420 430 440 450
LRIKRYVSKY TINPARANGI AEVVGSVEVG KWADLVLWDP AFFGVKPSLI
460 470 480 490 500
LKGGQIASAV MGDANASIPT PEPTLMRTMF GGHGAAPASN SITFMSQAAI
510 520 530 540 550
DAGVPQSLGL RKKVCPAHGV RRLTKADMAF NDATPALTVD PETYEVTVDG
560 570
EKVTCEPAEV LAMAQRYFLF
Length:570
Mass (Da):60,548
Last modified:May 1, 1999 - v1
Checksum:i167181A374257F21
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF048780 Genomic DNA. Translation: AAD13725.1.
AF056321 Genomic DNA. Translation: AAD13732.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF048780 Genomic DNA. Translation: AAD13725.1.
AF056321 Genomic DNA. Translation: AAD13732.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiM38.982.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.

Family and domain databases

Gene3Di2.30.40.10. 1 hit.
HAMAPiMF_01953. Urease_alpha.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genetic and physiologic characterization of urease of Actinomyces naeslundii."
    Morou-Bermudez E., Burne R.A.
    Infect. Immun. 67:504-512(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Strain: WVU45Imported.

Entry informationi

Entry nameiQ9Z369_ACTNA
AccessioniPrimary (citable) accession number: Q9Z369
Entry historyi
Integrated into UniProtKB/TrEMBL: May 1, 1999
Last sequence update: May 1, 1999
Last modified: May 11, 2016
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.