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Protein

Dynein light chain Tctex-type 1

Gene

Dynlt1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. Binds to transport cargos and is involved in apical cargo transport such as rhodopsin-bearing vesicles in polarized epithelia. Is involved in intracellular targeting of D-type retrovirus gag polyproteins to the cytoplasmic assembly site. May also be a accessory component of axonemal dynein (By similarity).By similarity
Plays a role in neuronal morphogenesis; the function is independent of cytoplasmic dynein and seems to be coupled to regulation of the actin cytoskeleton by enhancing Rac1 activity. Required for neurite outgrowth. The function in neurogenesis may be regulated by association with a G-protein beta-gamma dimer. May function as a receptor-independent activator of heterotrimeric G-protein signaling; the activation appears to be independent of a nucleotide exchange. Plays a role in regulating neurogenesis; inhibits the genesis of neurons from precursor cells during cortical development presumably by antagonizing ARHGEF2. Involved in the regulation of mitotic spindle orientation.2 Publications

GO - Molecular functioni

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • establishment of mitotic spindle orientation Source: UniProtKB
  • intracellular protein transport Source: UniProtKB
  • intracellular transport of viral protein in host cell Source: Ensembl
  • mitotic nuclear division Source: UniProtKB-KW
  • negative regulation of neurogenesis Source: UniProtKB
  • neuron projection morphogenesis Source: UniProtKB
  • regulation of cytoskeleton organization Source: UniProtKB
  • regulation of G-protein coupled receptor protein signaling pathway Source: UniProtKB
  • regulation of GTPase activity Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Motor protein

Keywords - Biological processi

Cell cycle, Cell division, Mitosis, Neurogenesis, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Dynein light chain Tctex-type 1
Alternative name(s):
Activator of G-protein signaling 2
Short name:
AGS2
T-complex testis-specific protein 1 homolog
Gene namesi
Name:Dynlt1
Synonyms:Tctel1, Tctex-1, Tctex1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi620261. Dynlt1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Dynein, Golgi apparatus, Microtubule

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi94 – 941T → A: No effect on interaction with dynein intermediate chain (DYNC1I1 or DYNC1I2). 1 Publication
Mutagenesisi94 – 941T → E: Impairs interaction with dynein intermediate chain (DYNC1I1 or DYNC1I2). 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 113113Dynein light chain Tctex-type 1PRO_0000195154Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Post-translational modificationi

Phosphorylated by BMPR2. The phosphorylation status is proposed to regulate the association with the cytoplasmic dynein complex and may have role in cytoplasmic dynein cargo release.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9Z336.
PRIDEiQ9Z336.

PTM databases

iPTMnetiQ9Z336.
PhosphoSiteiQ9Z336.

Expressioni

Gene expression databases

ExpressionAtlasiQ9Z336. baseline and differential.
GenevisibleiQ9Z336. RN.

Interactioni

Subunit structurei

Homodimer (By similarity). The cytoplasmic dynein 1 complex consists of two catalytic heavy chains (HCs) and a number of non-catalytic subunits presented by intermediate chains (ICs), light intermediate chains (LICs) and light chains (LCs); the composition seems to vary in respect to the IC, LIC and LC composition. The heavy chain homodimer serves as a scaffold for the probable homodimeric assembly of the respective non-catalytic subunits. The ICs and LICs bind directly to the HC dimer and dynein LCs assemble on the IC dimer. DYNLT1 and DYNLT3 compete for association with dynein IC (DYNC1I1 or DYNC1I2). Self-associates. Interacts with RHO. Interacts with DYNC1I1 and DYNC1I2 (By similarity). Interacts with DOC2A, DOC2B and SCN10A. Interacts with PVR. Interacts with SVIL isoform 2 (By similarity). Interacts with GNB1; the interaction occurs in presence of guanine nucleotide-binding protein G(T) subunit gamma; the interaction diminishes the association of DYNLT1 with dynein IC (DYNC1I1 or DYNC1I2). Interacts with GNB2, GNB3 and GNB5; the interactions occur in presence of guanine nucleotide-binding protein G(T) subunit gamma.By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Scn10aQ629682EBI-920359,EBI-1800320

Protein-protein interaction databases

BioGridi249705. 1 interaction.
IntActiQ9Z336. 2 interactions.
STRINGi10116.ENSRNOP00000024656.

Structurei

3D structure databases

ProteinModelPortaliQ9Z336.
SMRiQ9Z336. Positions 12-113.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni41 – 11373Interaction with GNB1Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG4081. Eukaryota.
ENOG4111NHR. LUCA.
GeneTreeiENSGT00390000013998.
HOGENOMiHOG000255148.
HOVERGENiHBG001217.
InParanoidiQ9Z336.
KOiK10420.
OMAiGNAYQHN.
OrthoDBiEOG7PK918.
PhylomeDBiQ9Z336.
TreeFamiTF313904.

Family and domain databases

InterProiIPR005334. Tctex.
[Graphical view]
PfamiPF03645. Tctex-1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z336-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEDFQASEET AFVVDEVSNI VKEAIESAIG GNAYQHSKVN QWTTNVVEQT
60 70 80 90 100
LSQLTKLGKP FKYIVTCVIM QKNGAGLHTA SSCFWDSSTD GSCTVRWENK
110
TMYCIVSAFG LSI
Length:113
Mass (Da):12,452
Last modified:May 1, 1999 - v1
Checksum:i722890BD6832F041
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010119 mRNA. Translation: BAA34532.1.
AJ131437 mRNA. Translation: CAC18728.1.
RefSeqiNP_112608.1. NM_031318.1.
UniGeneiRn.29901.

Genome annotation databases

EnsembliENSRNOT00000024656; ENSRNOP00000024656; ENSRNOG00000018207.
GeneIDi83462.
KEGGirno:83462.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB010119 mRNA. Translation: BAA34532.1.
AJ131437 mRNA. Translation: CAC18728.1.
RefSeqiNP_112608.1. NM_031318.1.
UniGeneiRn.29901.

3D structure databases

ProteinModelPortaliQ9Z336.
SMRiQ9Z336. Positions 12-113.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249705. 1 interaction.
IntActiQ9Z336. 2 interactions.
STRINGi10116.ENSRNOP00000024656.

PTM databases

iPTMnetiQ9Z336.
PhosphoSiteiQ9Z336.

Proteomic databases

PaxDbiQ9Z336.
PRIDEiQ9Z336.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000024656; ENSRNOP00000024656; ENSRNOG00000018207.
GeneIDi83462.
KEGGirno:83462.

Organism-specific databases

CTDi6993.
RGDi620261. Dynlt1.

Phylogenomic databases

eggNOGiKOG4081. Eukaryota.
ENOG4111NHR. LUCA.
GeneTreeiENSGT00390000013998.
HOGENOMiHOG000255148.
HOVERGENiHBG001217.
InParanoidiQ9Z336.
KOiK10420.
OMAiGNAYQHN.
OrthoDBiEOG7PK918.
PhylomeDBiQ9Z336.
TreeFamiTF313904.

Miscellaneous databases

PROiQ9Z336.

Gene expression databases

ExpressionAtlasiQ9Z336. baseline and differential.
GenevisibleiQ9Z336. RN.

Family and domain databases

InterProiIPR005334. Tctex.
[Graphical view]
PfamiPF03645. Tctex-1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Interaction of Doc2 with tctex-1, a light chain of cytoplasmic dynein. Implication in dynein-dependent vesicle transport."
    Nagano F., Orita S., Sasaki T., Naito A., Sakaguchi G., Maeda M., Watanabe T., Kominami E., Uchiyama Y., Takai Y.
    J. Biol. Chem. 273:30065-30068(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH DOC2A AND DOC2B.
    Strain: Sprague-Dawley.
    Tissue: Brain.
  2. Horiuchi M., Betz H.
    Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "Light chains of mammalian cytoplasmic dynein: identification and characterization of a family of LC8 light chains."
    Wilson M.J., Salata M.W., Susalka S.J., Pfister K.K.
    Cell Motil. Cytoskeleton 49:229-240(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE CYTOPLASMIC DYNEIN 1 COMPLEX.
  4. "Sensory neuron proteins interact with the intracellular domains of sodium channel NaV1.8."
    Malik-Hall M., Poon W.-Y.L., Baker M.D., Wood J.N., Okuse K.
    Brain Res. Mol. Brain Res. 110:298-304(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SCN10A, IDENTIFICATION IN A COMPLEX WITH SCN10A.
  5. "The dynein light chain Tctex-1 has a dynein-independent role in actin remodeling during neurite outgrowth."
    Chuang J.Z., Yeh T.Y., Bollati F., Conde C., Canavosio F., Caceres A., Sung C.H.
    Dev. Cell 9:75-86(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN REGULATION OF NEURONAL MORPHOGENESIS, MUTAGENESIS OF THR-94.
  6. "G protein beta gamma subunit interaction with the dynein light-chain component Tctex-1 regulates neurite outgrowth."
    Sachdev P., Menon S., Kastner D.B., Chuang J.Z., Yeh T.Y., Conde C., Caceres A., Sung C.H., Sakmar T.P.
    EMBO J. 26:2621-2632(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GNB1; GNB2; GNB3 AND GNB5, FUNCTION IN NEURONAL MORPHOGENESIS.

Entry informationi

Entry nameiDYLT1_RAT
AccessioniPrimary (citable) accession number: Q9Z336
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: May 1, 1999
Last modified: June 8, 2016
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.