Reviewed,
UniProtKB/Swiss-Prot Q9Z330 (DNMT1_RAT)
Last modified
July 7, 2009.
Version 83.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: DNA (cytosine-5)-methyltransferase 1 Short name=Dnmt1 EC=2.1.1.37 Alternative name(s): MCMT DNA methyltransferase I DNA MTase RnoIP Short name=M.RnoIP | ||
| Gene names |
| ||
| Organism | Rattus norvegicus (Rat) | ||
| Taxonomic identifier | 10116 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 1622 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Function | Methylates CpG residues. Preferentially methylates hemimethylated DNA. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2 By similarity. |
| Catalytic activity | S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine. |
| Subunit structure | Interacts with HDAC1 and with PCNA. Forms a complex with DMAP1 and HDAC2, with direct interaction. Forms also a stable complex with E2F1, BB1 and HDAC1. Binds MBD2 and MBD3. Component of complexes containing SUV39H1. Interacts with DNMT3A and DNMT3B By similarity. Binds annexin V Potential. Interacts with the PRC2/EED-EZH2 complex By similarity. |
| Subcellular location | |
| Tissue specificity | Isoforms 0 and 8 are highly expressed in placenta, brain, lung, spleen, kidney, heart, and at much lower levels in liver. Isoform 1 is expressed in cerebellum, isoform 2 in muscle and testis, isoform 3 in lung, isoform 4 in spleen and brain, and isoform 5 in brain. |
| Sequence similarities | Belongs to the C5-methyltransferase family. Contains 2 BAH domains. Contains 1 CXXC-type zinc finger. |
Ontologies
Binary interactions
With | Entry | #Exp. | IntAct | Notes |
|---|---|---|---|---|
| ANXA5 | P08758 | 1 | EBI-78342,EBI-296601 | From a different organism. |
Alternative products
| This entry describes 9 isoforms produced by alternative splicing. [Align] [Select] Note: Additional isoforms seem to exist. | ||||||
| Isoform 1 (identifier: Q9Z330-1) This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. | ||||||
| Isoform 2 (identifier: Q9Z330-2) Also known as: SF1; The sequence of this isoform differs from the canonical sequence as follows: 1323-1403: Missing. | ||||||
| Isoform 3 (identifier: Q9Z330-3) Also known as: SF2; The sequence of this isoform differs from the canonical sequence as follows: 1252-1482: Missing. | ||||||
| Isoform 4 (identifier: Q9Z330-4) Also known as: SF3; The sequence of this isoform differs from the canonical sequence as follows: 1226-1477: Missing. | ||||||
| Isoform 5 (identifier: Q9Z330-5) Also known as: SF4; The sequence of this isoform differs from the canonical sequence as follows: 1218-1430: QKGDVEMLCG...VHERAGGCRM → VC | ||||||
| Isoform 6 (identifier: Q9Z330-6) Also known as: SF5; The sequence of this isoform differs from the canonical sequence as follows: 1202-1410: Missing. | ||||||
| Isoform 7 (identifier: Q9Z330-7) Also known as: SF6; The sequence of this isoform differs from the canonical sequence as follows: 1259-1481: Missing. | ||||||
| Isoform 8 (identifier: Q9Z330-8) Also known as: SF7; The sequence of this isoform differs from the canonical sequence as follows: 1216-1504: Missing. | ||||||
| Isoform 9 (identifier: Q9Z330-9) Also known as: short; The sequence of this isoform differs from the canonical sequence as follows: 1-118: Missing. |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 1622 | 1622 | DNA (cytosine-5)-methyltransferase 1 | PRO_0000088036 | |||||
Regions | |||||||||
| Domain | 759 – 884 | 126 | BAH 1 | ||||||
| Domain | 977 – 1105 | 129 | BAH 2 | ||||||
| Repeat | 1114 – 1115 | 2 | 1 | ||||||
| Repeat | 1116 – 1117 | 2 | 2 | ||||||
| Repeat | 1118 – 1119 | 2 | 3 | ||||||
| Repeat | 1120 – 1121 | 2 | 4 | ||||||
| Repeat | 1122 – 1123 | 2 | 5 | ||||||
| Repeat | 1124 – 1125 | 2 | 6 | ||||||
| Repeat | 1126 – 1127 | 2 | 7; approximate | ||||||
| Zinc finger | 650 – 696 | 47 | CXXC-type | ||||||
| Region | 1 – 342 | 342 | Interaction with the PRC2/EED-EZH2 complex By similarity | ||||||
| Region | 1 – 145 | 145 | Interaction with DNMT3A By similarity | ||||||
| Region | 146 – 213 | 68 | Interaction with DNMT3B By similarity | ||||||
| Region | 304 – 610 | 307 | Interaction with the PRC2/EED-EZH2 complex By similarity | ||||||
| Region | 327 – 556 | 230 | DNA replication foci-targeting sequence | ||||||
| Region | 1114 – 1127 | 14 | 7 X 2 AA tandem repeats of K-G | ||||||
| Region | 1126 – 1622 | 497 | Interaction with the PRC2/EED-EZH2 complex By similarity | ||||||
| Region | 1144 – 1622 | 479 | Catalytic | ||||||
| Motif | 173 – 200 | 28 | Nuclear localization signal Potential | ||||||
| Compositional bias | 149 – 152 | 4 | Poly-Ser | ||||||
| Compositional bias | 269 – 274 | 6 | Poly-Asp | ||||||
| Compositional bias | 726 – 729 | 4 | Poly-Lys | ||||||
Sites | |||||||||
| Active site | 1231 | 1 | By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 149 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 151 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 400 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 515 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 718 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 736 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 958 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1110 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 1118 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 1120 | 1 | N6-acetyllysine By similarity | ||||||
| Modified residue | 1122 | 1 | N6-acetyllysine By similarity | ||||||
Natural variations | |||||||||
| Alternative sequence | 1 – 118 | 118 | Missing in isoform 9. | VSP_005620 | |||||
| Alternative sequence | 1202 – 1410 | 209 | Missing in isoform 6. | VSP_005622 | |||||
| Alternative sequence | 1216 – 1504 | 289 | Missing in isoform 8. | VSP_005623 | |||||
| Alternative sequence | 1218 – 1430 | 213 | QKGDV…GGCRM → VC in isoform 5. | VSP_005621 | |||||
| Alternative sequence | 1226 – 1477 | 252 | Missing in isoform 4. | VSP_005624 | |||||
| Alternative sequence | 1252 – 1482 | 231 | Missing in isoform 3. | VSP_005625 | |||||
| Alternative sequence | 1259 – 1481 | 223 | Missing in isoform 7. | VSP_005626 | |||||
| Alternative sequence | 1323 – 1403 | 81 | Missing in isoform 2. | VSP_005627 | |||||
Experimental info | |||||||||
| Sequence conflict | 17 – 25 | 9 | AGSLPDHVR → RQARPRPCP Ref.3 | ||||||
| Sequence conflict | 189 | 1 | A → V in BAA20854. Ref.3 | ||||||
| Sequence conflict | 1276 | 1 | F → S in BAA37118. Ref.1 | ||||||
| Sequence conflict | 1300 | 1 | T → I in AAD28102. Ref.4 | ||||||
| Sequence conflict | 1372 | 1 | M → V in BAA37118. Ref.1 | ||||||
| Sequence conflict | 1394 – 1428 | 35 | KWRAT…RAGGC → NGEPQSWFQRQLRGSHYQPI LRDHICKDMSALVAA Ref.1 | ||||||
Sequences
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
References
| [1] | "Expression of rat DNA (cytosine-5) methyltransferase (DNA MTase) in rodent trophoblast giant cells: molecular cloning and characterization of rat DNA MTase." Kimura H., Takeda T., Tanaka S., Ogawa T., Shiota K. Biochem. Biophys. Res. Commun. 253:495-501(1998) [PubMed: 9878564] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Strain: Sprague-Dawley. Tissue: Brain and Placenta. |
| [2] | "Multiple N-terminal isoforms of DNA (cytosine-5-)-methyltransferase in vivo." Deng J., Szyf M. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 1-144 (ISOFORMS 1 AND 9). Tissue: Brain. |
| [3] | "Molecular cloning and characterization of annexin V-binding proteins with highly hydrophilic peptide structure." Ohsawa K., Imai Y., Ito D., Kohsaka S. J. Neurochem. 67:89-97(1996) [PubMed: 8667030] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 17-356, IN VITRO BINDING TO ANNEXIN V. Strain: Wistar. Tissue: Brain. |
| [4] | "Multiple isoforms of DNA methyltransferase are encoded by the vertebrate cytosine DNA methyltransferase gene." Deng J., Szyf M. J. Biol. Chem. 273:22869-22872(1998) [PubMed: 9722504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1169-1517 (ISOFORMS 1; 2; 3; 4; 5; 6; 7 AND 8). |
Cross-references
Sequence databases | |
|---|---|
| AB012214 mRNA. Translation: BAA37118.1. AF116344 mRNA. Translation: AAD32541.1. AF116345 Genomic DNA. Translation: AAD32542.1. D64060 mRNA. Translation: BAA20854.1. AF083043 AF083042 Genomic DNA. Translation: AAD28102.1. | |
| IPI | IPI00231930. IPI00231931. IPI00231933. IPI00231934. IPI00231935. IPI00231936. IPI00231937. IPI00231938. IPI00327802. |
| PIR | JE0378. |
| UniGene | Rn.218315 Rn.6955 |
3D structure databases | |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | Q9Z330. 2 interactions. |
Protein family/group databases | |
| REBASE | 11410. M.RraDnmtI. 3019. M.RnoDnmt1. |
PTM databases | |
| PhosphoSite | Q9Z330. |
Proteomic databases | |
| PRIDE | Q9Z330. |
Organism-specific databases | |
| RGD | 620979. Dnmt1. |
Phylogenomic databases | |
| HOVERGEN | Q9Z330. |
Enzyme and pathway databases | |
| BioCyc | MetaCyc:MON-8581. |
| BRENDA | 2.1.1.37. 248. |
Family and domain databases | |
| InterPro | IPR001025. BAH. IPR001525. C5_DNA_meth. IPR018117. C5_DNA_meth_AS. IPR010506. DMAP1_bd. IPR017198. DNA_C5-MeTrfase_1. IPR002857. Znf_CXXC. [Graphical view] |
| PANTHER | PTHR10629. C5_DNA_meth. 1 hit. |
| Pfam | PF01426. BAH. 2 hits. PF06464. DMAP_binding. 1 hit. PF00145. DNA_methylase. 3 hits. PF02008. zf-CXXC. 1 hit. [Graphical view] |
| PIRSF | PIRSF037404. DNMT1. 1 hit. |
| PRINTS | PR00105. C5METTRFRASE. |
| SMART | SM00439. BAH. 2 hits. [Graphical view] |
| TIGRFAMs | TIGR00675. dcm. 1 hit. |
| PROSITE | PS51038. BAH. 2 hits. PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. PS51058. ZF_CXXC. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 616655. |
Entry information
| Entry name | DNMT1_RAT | ||||||||
| Accession | Primary (citable) accession number: Q9Z330 Secondary accession number(s): P70487 Q9WU57 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||

Clusters with


