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Q9Z321

- TOP3B_MOUSE

UniProt

Q9Z321 - TOP3B_MOUSE

Protein

DNA topoisomerase 3-beta-1

Gene

Top3b

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. Possesses negatively supercoiled DNA relaxing activity.By similarity

    Catalytic activityi

    ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotations

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei336 – 3361O-(5'-phospho-DNA)-tyrosine intermediateBy similarity

    GO - Molecular functioni

    1. DNA binding Source: UniProtKB-KW
    2. DNA topoisomerase type I activity Source: UniProtKB-EC

    GO - Biological processi

    1. chromosome segregation Source: MGI
    2. DNA topological change Source: InterPro

    Keywords - Molecular functioni

    Isomerase, Topoisomerase

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA topoisomerase 3-beta-1 (EC:5.99.1.2)
    Alternative name(s):
    DNA topoisomerase III beta-1
    Gene namesi
    Name:Top3b
    Synonyms:Top3b1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

    Organism-specific databases

    MGIiMGI:1333803. Top3b.

    Subcellular locationi

    GO - Cellular componenti

    1. condensed chromosome Source: MGI

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 862862DNA topoisomerase 3-beta-1PRO_0000145193Add
    BLAST

    Proteomic databases

    MaxQBiQ9Z321.
    PaxDbiQ9Z321.
    PRIDEiQ9Z321.

    PTM databases

    PhosphoSiteiQ9Z321.

    Expressioni

    Tissue specificityi

    Highly expressed in testis.

    Gene expression databases

    ArrayExpressiQ9Z321.
    BgeeiQ9Z321.
    CleanExiMM_TOP3B.
    GenevestigatoriQ9Z321.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z321.
    SMRiQ9Z321. Positions 1-633.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 153151ToprimPROSITE-ProRule annotationsAdd
    BLAST

    Sequence similaritiesi

    Belongs to the type IA topoisomerase family.Curated
    Contains 1 Toprim domain.PROSITE-ProRule annotations

    Phylogenomic databases

    eggNOGiCOG0550.
    GeneTreeiENSGT00530000063430.
    HOGENOMiHOG000184378.
    HOVERGENiHBG059857.
    InParanoidiQ9Z321.
    KOiK03165.
    OMAiCQHSLSM.
    OrthoDBiEOG7RBZ7N.
    PhylomeDBiQ9Z321.
    TreeFamiTF105288.

    Family and domain databases

    Gene3Di1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    InterProiIPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR006171. Toprim_domain.
    [Graphical view]
    PANTHERiPTHR11390. PTHR11390. 1 hit.
    PfamiPF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    [Graphical view]
    PRINTSiPR00417. PRTPISMRASEI.
    SMARTiSM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view]
    SUPFAMiSSF56712. SSF56712. 1 hit.
    PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Sequencei

    Sequence statusi: Complete.

    Q9Z321-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKTVLMVAEK PSLAQSIAKI LSRGNMSSHK GLNGACSVHK YTGTFAGQPV    50
    HFKMTSVCGH VMTLDFLGKY NKWDKVDPAE LFSQAPTEKK EANPKLNMVK 100
    FLQVEGRGCD YVVLWLDCDK EGENICFEVL DAVLPVMNNA HNGEKTVFRA 150
    RFSSITDTDI CNAMTRLSEP DHNEALSVDA RQELDLRIGC AFTRFQTKYF 200
    QGKYGDLDSS LISFGPCQTP TLGFCVERHD KIQSFKPETY WVLQAKVHTD 250
    KEESLLLDWD RVRVFDWEIA QMFLNMTKLE KEAWVEATSR KEKAKQRPLA 300
    LNTVEMLRVA SSALGMGPQH AMQIAERLYT QGYISYPRTE TTHYPENFDL 350
    KGSLRQQANH PYWADSVKQL LAEGINRPRK GHDAGDHPPI TPMKSATEAE 400
    LGGDAWRLYE YITRHFIATV SHDCKYLQST ISFRIGPEHF TCMGKTVISP 450
    GFTEIMPWQS VPLEESLPTC QKGDTFTVGE VKMLEKQTSP PDYLTEAELI 500
    TLMEKHGIGT DASIPVHINN ICQRNYVTVE SGRRLKPTNL GIVLVHGYYK 550
    IDAELVLPTI RSAVEKQLNL IAQGKADYHQ VLGHTLDIFK RKFHYFVDSI 600
    AGMDELMEVS FSPLAATGKP LSRCGKCHRF MKYIQAKPSR LHCSHCDETY 650
    TLPQNGTIKL YKELRCPLDD FELVLWSSGS RGKSYPLCPY CYNHPPFRDM 700
    KKGMGCNECT HPTCQHSLSM LGIGQCVECE NGVLVLDPTS GPKWKVACNT 750
    CNVVAHCFEN AHRVRVSADT CNTCEAALLD VDFNKAKSPL PGNETQHTGC 800
    IFCDPVFQEL VELKHAASCH PMHRGGPGRR QGRGRGRGRR PPGKPNPRRP 850
    KDKMSALAAY FV 862
    Length:862
    Mass (Da):96,949
    Last modified:May 1, 1999 - v1
    Checksum:iD2C05429F79FD5CC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB013603 mRNA. Translation: BAA34227.1.
    BC031723 mRNA. Translation: AAH31723.1.
    CCDSiCCDS27990.1.
    RefSeqiNP_035754.1. NM_011624.2.
    XP_006522054.1. XM_006521991.1.
    XP_006522055.1. XM_006521992.1.
    XP_006522056.1. XM_006521993.1.
    XP_006522057.1. XM_006521994.1.
    XP_006522058.1. XM_006521995.1.
    XP_006522059.1. XM_006521996.1.
    UniGeneiMm.326089.

    Genome annotation databases

    EnsembliENSMUST00000023465; ENSMUSP00000023465; ENSMUSG00000022779.
    ENSMUST00000119787; ENSMUSP00000112913; ENSMUSG00000022779.
    GeneIDi21976.
    KEGGimmu:21976.
    UCSCiuc007yjj.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB013603 mRNA. Translation: BAA34227.1 .
    BC031723 mRNA. Translation: AAH31723.1 .
    CCDSi CCDS27990.1.
    RefSeqi NP_035754.1. NM_011624.2.
    XP_006522054.1. XM_006521991.1.
    XP_006522055.1. XM_006521992.1.
    XP_006522056.1. XM_006521993.1.
    XP_006522057.1. XM_006521994.1.
    XP_006522058.1. XM_006521995.1.
    XP_006522059.1. XM_006521996.1.
    UniGenei Mm.326089.

    3D structure databases

    ProteinModelPortali Q9Z321.
    SMRi Q9Z321. Positions 1-633.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q9Z321.

    Proteomic databases

    MaxQBi Q9Z321.
    PaxDbi Q9Z321.
    PRIDEi Q9Z321.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000023465 ; ENSMUSP00000023465 ; ENSMUSG00000022779 .
    ENSMUST00000119787 ; ENSMUSP00000112913 ; ENSMUSG00000022779 .
    GeneIDi 21976.
    KEGGi mmu:21976.
    UCSCi uc007yjj.1. mouse.

    Organism-specific databases

    CTDi 8940.
    MGIi MGI:1333803. Top3b.

    Phylogenomic databases

    eggNOGi COG0550.
    GeneTreei ENSGT00530000063430.
    HOGENOMi HOG000184378.
    HOVERGENi HBG059857.
    InParanoidi Q9Z321.
    KOi K03165.
    OMAi CQHSLSM.
    OrthoDBi EOG7RBZ7N.
    PhylomeDBi Q9Z321.
    TreeFami TF105288.

    Miscellaneous databases

    ChiTaRSi TOP3B. mouse.
    NextBioi 301682.
    PROi Q9Z321.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9Z321.
    Bgeei Q9Z321.
    CleanExi MM_TOP3B.
    Genevestigatori Q9Z321.

    Family and domain databases

    Gene3Di 1.10.460.10. 2 hits.
    2.70.20.10. 2 hits.
    3.40.50.140. 1 hit.
    InterProi IPR000380. Topo_IA.
    IPR003601. Topo_IA_2.
    IPR023406. Topo_IA_AS.
    IPR013497. Topo_IA_cen.
    IPR013824. Topo_IA_cen_sub1.
    IPR013825. Topo_IA_cen_sub2.
    IPR023405. Topo_IA_core_domain.
    IPR003602. Topo_IA_DNA-bd.
    IPR006171. Toprim_domain.
    [Graphical view ]
    PANTHERi PTHR11390. PTHR11390. 1 hit.
    Pfami PF01131. Topoisom_bac. 1 hit.
    PF01751. Toprim. 1 hit.
    [Graphical view ]
    PRINTSi PR00417. PRTPISMRASEI.
    SMARTi SM00437. TOP1Ac. 1 hit.
    SM00436. TOP1Bc. 1 hit.
    SM00493. TOPRIM. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56712. SSF56712. 1 hit.
    PROSITEi PS00396. TOPOISOMERASE_I_PROK. 1 hit.
    PS50880. TOPRIM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of cDNA encoding a novel mouse DNA topoisomerase III (Topo IIIbeta) possessing negatively supercoiled DNA relaxing activity, whose message is highly expressed in the testis."
      Seki T., Seki M., Onodera R., Katada T., Enomoto T.
      J. Biol. Chem. 273:28553-28556(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Testis.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N-3.
      Tissue: Mammary gland.

    Entry informationi

    Entry nameiTOP3B_MOUSE
    AccessioniPrimary (citable) accession number: Q9Z321
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

    Similar proteinsi