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Protein

DNA topoisomerase 3-beta-1

Gene

Top3b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity.By similarity

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei336 – 3361O-(5'-phospho-DNA)-tyrosine intermediateBy similarity

GO - Molecular functioni

  1. DNA binding Source: UniProtKB-KW
  2. DNA topoisomerase type I activity Source: UniProtKB-EC
  3. poly(A) RNA binding Source: MGI

GO - Biological processi

  1. chromosome segregation Source: MGI
  2. DNA topological change Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Topoisomerase

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3-beta-1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase III beta-1
Gene namesi
Name:Top3b
Synonyms:Top3b1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 16

Organism-specific databases

MGIiMGI:1333803. Top3b.

Subcellular locationi

GO - Cellular componenti

  1. condensed chromosome Source: MGI
  2. nucleoplasm Source: MGI
  3. nucleus Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 862862DNA topoisomerase 3-beta-1PRO_0000145193Add
BLAST

Proteomic databases

MaxQBiQ9Z321.
PaxDbiQ9Z321.
PRIDEiQ9Z321.

PTM databases

PhosphoSiteiQ9Z321.

Expressioni

Tissue specificityi

Highly expressed in testis.

Gene expression databases

BgeeiQ9Z321.
CleanExiMM_TOP3B.
ExpressionAtlasiQ9Z321. baseline and differential.
GenevestigatoriQ9Z321.

Structurei

3D structure databases

ProteinModelPortaliQ9Z321.
SMRiQ9Z321. Positions 1-633.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 153151ToprimPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the type IA topoisomerase family.Curated
Contains 1 Toprim domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0550.
GeneTreeiENSGT00530000063430.
HOGENOMiHOG000184378.
HOVERGENiHBG059857.
InParanoidiQ9Z321.
KOiK03165.
OMAiHFFENAH.
OrthoDBiEOG7RBZ7N.
PhylomeDBiQ9Z321.
TreeFamiTF105288.

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z321-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTVLMVAEK PSLAQSIAKI LSRGNMSSHK GLNGACSVHK YTGTFAGQPV
60 70 80 90 100
HFKMTSVCGH VMTLDFLGKY NKWDKVDPAE LFSQAPTEKK EANPKLNMVK
110 120 130 140 150
FLQVEGRGCD YVVLWLDCDK EGENICFEVL DAVLPVMNNA HNGEKTVFRA
160 170 180 190 200
RFSSITDTDI CNAMTRLSEP DHNEALSVDA RQELDLRIGC AFTRFQTKYF
210 220 230 240 250
QGKYGDLDSS LISFGPCQTP TLGFCVERHD KIQSFKPETY WVLQAKVHTD
260 270 280 290 300
KEESLLLDWD RVRVFDWEIA QMFLNMTKLE KEAWVEATSR KEKAKQRPLA
310 320 330 340 350
LNTVEMLRVA SSALGMGPQH AMQIAERLYT QGYISYPRTE TTHYPENFDL
360 370 380 390 400
KGSLRQQANH PYWADSVKQL LAEGINRPRK GHDAGDHPPI TPMKSATEAE
410 420 430 440 450
LGGDAWRLYE YITRHFIATV SHDCKYLQST ISFRIGPEHF TCMGKTVISP
460 470 480 490 500
GFTEIMPWQS VPLEESLPTC QKGDTFTVGE VKMLEKQTSP PDYLTEAELI
510 520 530 540 550
TLMEKHGIGT DASIPVHINN ICQRNYVTVE SGRRLKPTNL GIVLVHGYYK
560 570 580 590 600
IDAELVLPTI RSAVEKQLNL IAQGKADYHQ VLGHTLDIFK RKFHYFVDSI
610 620 630 640 650
AGMDELMEVS FSPLAATGKP LSRCGKCHRF MKYIQAKPSR LHCSHCDETY
660 670 680 690 700
TLPQNGTIKL YKELRCPLDD FELVLWSSGS RGKSYPLCPY CYNHPPFRDM
710 720 730 740 750
KKGMGCNECT HPTCQHSLSM LGIGQCVECE NGVLVLDPTS GPKWKVACNT
760 770 780 790 800
CNVVAHCFEN AHRVRVSADT CNTCEAALLD VDFNKAKSPL PGNETQHTGC
810 820 830 840 850
IFCDPVFQEL VELKHAASCH PMHRGGPGRR QGRGRGRGRR PPGKPNPRRP
860
KDKMSALAAY FV
Length:862
Mass (Da):96,949
Last modified:May 1, 1999 - v1
Checksum:iD2C05429F79FD5CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB013603 mRNA. Translation: BAA34227.1.
BC031723 mRNA. Translation: AAH31723.1.
CCDSiCCDS27990.1.
RefSeqiNP_035754.1. NM_011624.2.
XP_006522054.1. XM_006521991.1.
XP_006522055.1. XM_006521992.1.
XP_006522056.1. XM_006521993.1.
XP_006522057.1. XM_006521994.1.
XP_006522058.1. XM_006521995.1.
XP_006522059.1. XM_006521996.1.
UniGeneiMm.326089.

Genome annotation databases

EnsembliENSMUST00000023465; ENSMUSP00000023465; ENSMUSG00000022779.
ENSMUST00000119787; ENSMUSP00000112913; ENSMUSG00000022779.
GeneIDi21976.
KEGGimmu:21976.
UCSCiuc007yjj.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB013603 mRNA. Translation: BAA34227.1.
BC031723 mRNA. Translation: AAH31723.1.
CCDSiCCDS27990.1.
RefSeqiNP_035754.1. NM_011624.2.
XP_006522054.1. XM_006521991.1.
XP_006522055.1. XM_006521992.1.
XP_006522056.1. XM_006521993.1.
XP_006522057.1. XM_006521994.1.
XP_006522058.1. XM_006521995.1.
XP_006522059.1. XM_006521996.1.
UniGeneiMm.326089.

3D structure databases

ProteinModelPortaliQ9Z321.
SMRiQ9Z321. Positions 1-633.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ9Z321.

Proteomic databases

MaxQBiQ9Z321.
PaxDbiQ9Z321.
PRIDEiQ9Z321.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023465; ENSMUSP00000023465; ENSMUSG00000022779.
ENSMUST00000119787; ENSMUSP00000112913; ENSMUSG00000022779.
GeneIDi21976.
KEGGimmu:21976.
UCSCiuc007yjj.1. mouse.

Organism-specific databases

CTDi8940.
MGIiMGI:1333803. Top3b.

Phylogenomic databases

eggNOGiCOG0550.
GeneTreeiENSGT00530000063430.
HOGENOMiHOG000184378.
HOVERGENiHBG059857.
InParanoidiQ9Z321.
KOiK03165.
OMAiHFFENAH.
OrthoDBiEOG7RBZ7N.
PhylomeDBiQ9Z321.
TreeFamiTF105288.

Miscellaneous databases

ChiTaRSiTop3b. mouse.
NextBioi301682.
PROiQ9Z321.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Z321.
CleanExiMM_TOP3B.
ExpressionAtlasiQ9Z321. baseline and differential.
GenevestigatoriQ9Z321.

Family and domain databases

Gene3Di1.10.460.10. 2 hits.
2.70.20.10. 2 hits.
3.40.50.140. 1 hit.
InterProiIPR000380. Topo_IA.
IPR003601. Topo_IA_2.
IPR023406. Topo_IA_AS.
IPR013497. Topo_IA_cen.
IPR013824. Topo_IA_cen_sub1.
IPR013825. Topo_IA_cen_sub2.
IPR023405. Topo_IA_core_domain.
IPR003602. Topo_IA_DNA-bd_dom.
IPR006171. Toprim_domain.
[Graphical view]
PANTHERiPTHR11390. PTHR11390. 1 hit.
PfamiPF01131. Topoisom_bac. 1 hit.
PF01751. Toprim. 1 hit.
[Graphical view]
PRINTSiPR00417. PRTPISMRASEI.
SMARTiSM00437. TOP1Ac. 1 hit.
SM00436. TOP1Bc. 1 hit.
SM00493. TOPRIM. 1 hit.
[Graphical view]
SUPFAMiSSF56712. SSF56712. 1 hit.
PROSITEiPS00396. TOPOISOMERASE_I_PROK. 1 hit.
PS50880. TOPRIM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of cDNA encoding a novel mouse DNA topoisomerase III (Topo IIIbeta) possessing negatively supercoiled DNA relaxing activity, whose message is highly expressed in the testis."
    Seki T., Seki M., Onodera R., Katada T., Enomoto T.
    J. Biol. Chem. 273:28553-28556(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N-3.
    Tissue: Mammary gland.

Entry informationi

Entry nameiTOP3B_MOUSE
AccessioniPrimary (citable) accession number: Q9Z321
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: March 4, 2015
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.