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Protein

DNA topoisomerase 3-beta-1

Gene

Top3b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Possesses negatively supercoiled DNA relaxing activity.By similarity

Catalytic activityi

ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei336O-(5'-phospho-DNA)-tyrosine intermediateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • chromosome segregation Source: MGI
  • DNA topological change Source: GO_Central

Keywordsi

Molecular functionDNA-binding, Isomerase, Topoisomerase

Names & Taxonomyi

Protein namesi
Recommended name:
DNA topoisomerase 3-beta-1 (EC:5.99.1.2)
Alternative name(s):
DNA topoisomerase III beta-1
Gene namesi
Name:Top3b
Synonyms:Top3b1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1333803 Top3b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001451931 – 862DNA topoisomerase 3-beta-1Add BLAST862

Proteomic databases

EPDiQ9Z321
MaxQBiQ9Z321
PaxDbiQ9Z321
PRIDEiQ9Z321

PTM databases

iPTMnetiQ9Z321
PhosphoSitePlusiQ9Z321

Expressioni

Tissue specificityi

Highly expressed in testis.

Gene expression databases

BgeeiENSMUSG00000022779
CleanExiMM_TOP3B
ExpressionAtlasiQ9Z321 baseline and differential
GenevisibleiQ9Z321 MM

Interactioni

Protein-protein interaction databases

BioGridi204279, 2 interactors
IntActiQ9Z321, 2 interactors
STRINGi10090.ENSMUSP00000023465

Structurei

3D structure databases

ProteinModelPortaliQ9Z321
SMRiQ9Z321
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 153ToprimPROSITE-ProRule annotationAdd BLAST151

Sequence similaritiesi

Belongs to the type IA topoisomerase family.Curated

Phylogenomic databases

eggNOGiKOG1956 Eukaryota
COG0550 LUCA
GeneTreeiENSGT00530000063430
HOGENOMiHOG000184378
HOVERGENiHBG059857
InParanoidiQ9Z321
KOiK03165
OMAiTYPRVDT
OrthoDBiEOG091G01EW
PhylomeDBiQ9Z321
TreeFamiTF105288

Family and domain databases

CDDicd00186 TOP1Ac, 1 hit
cd03362 TOPRIM_TopoIA_TopoIII, 1 hit
Gene3Di1.10.290.10, 1 hit
1.10.460.10, 2 hits
2.70.20.10, 2 hits
InterProiView protein in InterPro
IPR000380 Topo_IA
IPR003601 Topo_IA_2
IPR023406 Topo_IA_AS
IPR013497 Topo_IA_cen
IPR013824 Topo_IA_cen_sub1
IPR013825 Topo_IA_cen_sub2
IPR013826 Topo_IA_cen_sub3
IPR023405 Topo_IA_core_domain
IPR003602 Topo_IA_DNA-bd_dom
IPR006171 TOPRIM_domain
IPR034144 TOPRIM_TopoIII
PANTHERiPTHR11390 PTHR11390, 1 hit
PfamiView protein in Pfam
PF01131 Topoisom_bac, 1 hit
PF01751 Toprim, 1 hit
PRINTSiPR00417 PRTPISMRASEI
SMARTiView protein in SMART
SM00437 TOP1Ac, 1 hit
SM00436 TOP1Bc, 1 hit
SM00493 TOPRIM, 1 hit
SUPFAMiSSF56712 SSF56712, 1 hit
PROSITEiView protein in PROSITE
PS00396 TOPOISOMERASE_I_PROK, 1 hit
PS50880 TOPRIM, 1 hit

Sequencei

Sequence statusi: Complete.

Q9Z321-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTVLMVAEK PSLAQSIAKI LSRGNMSSHK GLNGACSVHK YTGTFAGQPV
60 70 80 90 100
HFKMTSVCGH VMTLDFLGKY NKWDKVDPAE LFSQAPTEKK EANPKLNMVK
110 120 130 140 150
FLQVEGRGCD YVVLWLDCDK EGENICFEVL DAVLPVMNNA HNGEKTVFRA
160 170 180 190 200
RFSSITDTDI CNAMTRLSEP DHNEALSVDA RQELDLRIGC AFTRFQTKYF
210 220 230 240 250
QGKYGDLDSS LISFGPCQTP TLGFCVERHD KIQSFKPETY WVLQAKVHTD
260 270 280 290 300
KEESLLLDWD RVRVFDWEIA QMFLNMTKLE KEAWVEATSR KEKAKQRPLA
310 320 330 340 350
LNTVEMLRVA SSALGMGPQH AMQIAERLYT QGYISYPRTE TTHYPENFDL
360 370 380 390 400
KGSLRQQANH PYWADSVKQL LAEGINRPRK GHDAGDHPPI TPMKSATEAE
410 420 430 440 450
LGGDAWRLYE YITRHFIATV SHDCKYLQST ISFRIGPEHF TCMGKTVISP
460 470 480 490 500
GFTEIMPWQS VPLEESLPTC QKGDTFTVGE VKMLEKQTSP PDYLTEAELI
510 520 530 540 550
TLMEKHGIGT DASIPVHINN ICQRNYVTVE SGRRLKPTNL GIVLVHGYYK
560 570 580 590 600
IDAELVLPTI RSAVEKQLNL IAQGKADYHQ VLGHTLDIFK RKFHYFVDSI
610 620 630 640 650
AGMDELMEVS FSPLAATGKP LSRCGKCHRF MKYIQAKPSR LHCSHCDETY
660 670 680 690 700
TLPQNGTIKL YKELRCPLDD FELVLWSSGS RGKSYPLCPY CYNHPPFRDM
710 720 730 740 750
KKGMGCNECT HPTCQHSLSM LGIGQCVECE NGVLVLDPTS GPKWKVACNT
760 770 780 790 800
CNVVAHCFEN AHRVRVSADT CNTCEAALLD VDFNKAKSPL PGNETQHTGC
810 820 830 840 850
IFCDPVFQEL VELKHAASCH PMHRGGPGRR QGRGRGRGRR PPGKPNPRRP
860
KDKMSALAAY FV
Length:862
Mass (Da):96,949
Last modified:May 1, 1999 - v1
Checksum:iD2C05429F79FD5CC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB013603 mRNA Translation: BAA34227.1
BC031723 mRNA Translation: AAH31723.1
CCDSiCCDS27990.1
RefSeqiNP_001313505.1, NM_001326576.1
NP_035754.1, NM_011624.3
XP_006522054.1, XM_006521991.3
XP_006522055.1, XM_006521992.3
XP_006522057.1, XM_006521994.3
XP_006522058.1, XM_006521995.2
XP_017172426.1, XM_017316937.1
UniGeneiMm.326089

Genome annotation databases

EnsembliENSMUST00000023465; ENSMUSP00000023465; ENSMUSG00000022779
ENSMUST00000119787; ENSMUSP00000112913; ENSMUSG00000022779
GeneIDi21976
KEGGimmu:21976
UCSCiuc007yjj.1 mouse

Similar proteinsi

Entry informationi

Entry nameiTOP3B_MOUSE
AccessioniPrimary (citable) accession number: Q9Z321
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: March 28, 2018
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health