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Q9Z311 (MECR_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Trans-2-enoyl-CoA reductase, mitochondrial

EC=1.3.1.38
Alternative name(s):
Nuclear receptor-binding factor 1
Short name=NRBF-1
Gene names
Name:Mecr
Synonyms:Nrbf1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length373 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Oxidoreductase with a preference for short and medium chain substrates, including trans-2-hexenoyl-CoA (C6), trans-2-decenoyl-CoA (C10), and trans-2-hexadecenoyl-CoA (C16). May play a role in mitochondrial fatty acid synthesis By similarity.

Catalytic activity

Acyl-CoA + NADP+ = trans-2,3-dehydroacyl-CoA + NADPH.

Subunit structure

Homodimer By similarity.

Subcellular location

Mitochondrion Ref.2.

Sequence similarities

Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.

Caution

Was originally (Ref.1) thought to be a nuclear protein that interact with nuclear receptor. However, it was shown later to be mitochondrial (Ref.2), a function related to nuclear receptors being unsure.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5353Mitochondrion Potential
Chain54 – 373320Trans-2-enoyl-CoA reductase, mitochondrial
PRO_0000000890

Regions

Nucleotide binding193 – 1964NADP By similarity
Nucleotide binding216 – 2183NADP By similarity
Nucleotide binding285 – 2884NADP By similarity
Nucleotide binding310 – 3123NADP By similarity

Sites

Binding site1671NADP By similarity
Binding site3681NADP By similarity

Amino acid modifications

Modified residue611N6-acetyllysine; alternate By similarity
Modified residue611N6-succinyllysine; alternate By similarity
Modified residue2521N6-acetyllysine; alternate By similarity
Modified residue2521N6-succinyllysine; alternate By similarity
Modified residue2671N6-acetyllysine; alternate By similarity
Modified residue2671N6-succinyllysine; alternate By similarity
Modified residue3161N6-succinyllysine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9Z311 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 94D5A057D666320D

FASTA37340,327
        10         20         30         40         50         60 
MLVSRRLTGA RARAPLLASL LEAWCRQGRT TSSYSAFSEP SHVRALVYGN HGDPAKVIQL 

        70         80         90        100        110        120 
KNLELTAVEG SDVHVKMLAA PINPSDINMI QGNYGLLPKL PAVGGNEGVG QVIAVGSSVS 

       130        140        150        160        170        180 
GLKPGDWVIP ANAGLGTWRT EAVFSEEALI GVPKDIPLQS AATLGVNPCT AYRMLVDFEQ 

       190        200        210        220        230        240 
LQPGDSVIQN ASNSGVGQAV IQIASALGLK TINVIRDRPD IKKLTDRLKD LGADYVLTEE 

       250        260        270        280        290        300 
ELRMPETKNI FKDLPLPRLA LNCVGGKSST ELLRHLAPGG TMVTYGGMAK QPVTASVSML 

       310        320        330        340        350        360 
IFKDLKLRGF WLSQWKKNHS PDEFKELILI LCNLIRQGQL TAPAWSGIPL QDYQQALEAS 

       370 
MKPFVSLKQI LTM 

« Hide

References

[1]"Nuclear receptor binding factor-1 (NRBF-1), a protein interacting with a wide spectrum of nuclear hormone receptors."
Masuda N., Yasumo H., Furusawa T., Tsukamoto T., Sadano H., Osumi T.
Gene 221:225-233(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Characterization of 2-enoyl thioester reductase from mammals: an ortholog of Ybr026p/Mrf1'p of the yeast mitochondrial fatty acid synthesis type II."
Miinalainen I.J., Chen Z.-J., Torkko J.M., Pirilae P.L., Sormunen R.T., Bergmann U., Qin Y.-M., Hiltunen J.K.
J. Biol. Chem. 278:20154-20161(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB015724 mRNA. Translation: BAA34804.1.
RefSeqNP_058905.1. NM_017209.1.
UniGeneRn.15375.

3D structure databases

ProteinModelPortalQ9Z311.
SMRQ9Z311. Positions 38-373.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000031375.

PTM databases

PhosphoSiteQ9Z311.

Proteomic databases

PaxDbQ9Z311.
PRIDEQ9Z311.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID29470.
KEGGrno:29470.
UCSCRGD:3208. rat.

Organism-specific databases

CTD51102.
RGD3208. Mecr.

Phylogenomic databases

eggNOGCOG0604.
HOGENOMHOG000294683.
HOVERGENHBG052446.
InParanoidQ9Z311.
KOK07512.
PhylomeDBQ9Z311.

Gene expression databases

GenevestigatorQ9Z311.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProIPR013149. ADH_C.
IPR013154. ADH_GroES-like.
IPR002085. ADH_SF_Zn-type.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR11695. PTHR11695. 1 hit.
PfamPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SUPFAMSSF50129. SSF50129. 1 hit.
ProtoNetSearch...

Other

NextBio609292.
PROQ9Z311.

Entry information

Entry nameMECR_RAT
AccessionPrimary (citable) accession number: Q9Z311
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: May 1, 1999
Last modified: April 16, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families