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Protein

Trans-2-enoyl-CoA reductase, mitochondrial

Gene

Mecr

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Oxidoreductase with a preference for short and medium chain substrates, including trans-2-hexenoyl-CoA (C6), trans-2-decenoyl-CoA (C10), and trans-2-hexadecenoyl-CoA (C16). May play a role in mitochondrial fatty acid synthesis (By similarity).By similarity

Catalytic activityi

Acyl-CoA + NADP+ = trans-2,3-dehydroacyl-CoA + NADPH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei167 – 1671NADPBy similarity
Binding sitei368 – 3681NADPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi193 – 1964NADPBy similarity
Nucleotide bindingi216 – 2183NADPBy similarity
Nucleotide bindingi285 – 2884NADPBy similarity
Nucleotide bindingi310 – 3123NADPBy similarity

GO - Molecular functioni

  1. receptor binding Source: RGD
  2. trans-2-enoyl-CoA reductase (NADPH) activity Source: UniProtKB
  3. zinc ion binding Source: InterPro

GO - Biological processi

  1. fatty acid biosynthetic process Source: UniProtKB-KW
  2. fatty acid metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Keywords - Ligandi

NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Trans-2-enoyl-CoA reductase, mitochondrial (EC:1.3.1.38)
Alternative name(s):
Nuclear receptor-binding factor 1
Short name:
NRBF-1
Gene namesi
Name:Mecr
Synonyms:Nrbf1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3208. Mecr.

Subcellular locationi

Isoform 1 : Mitochondrion 1 Publication
Isoform 2 : Cytoplasm 1 Publication. Nucleus 1 Publication

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5353MitochondrionSequence AnalysisAdd
BLAST
Chaini54 – 373320Trans-2-enoyl-CoA reductase, mitochondrialPRO_0000000890Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei61 – 611N6-acetyllysine; alternateBy similarity
Modified residuei61 – 611N6-succinyllysine; alternateBy similarity
Modified residuei252 – 2521N6-acetyllysine; alternateBy similarity
Modified residuei252 – 2521N6-succinyllysine; alternateBy similarity
Modified residuei267 – 2671N6-acetyllysine; alternateBy similarity
Modified residuei267 – 2671N6-succinyllysine; alternateBy similarity
Modified residuei316 – 3161N6-succinyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ9Z311.
PRIDEiQ9Z311.

PTM databases

PhosphoSiteiQ9Z311.

Expressioni

Gene expression databases

GenevestigatoriQ9Z311.

Interactioni

Subunit structurei

Homodimer. Isoform 2 interacts with PPARA in the nucleus and increases its activity.By similarity1 Publication

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000031375.

Structurei

3D structure databases

ProteinModelPortaliQ9Z311.
SMRiQ9Z311. Positions 38-373.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0604.
HOGENOMiHOG000294683.
HOVERGENiHBG052446.
InParanoidiQ9Z311.
KOiK07512.
OMAiCRAWGIN.
OrthoDBiEOG78M024.
PhylomeDBiQ9Z311.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_GroES-like.
IPR002085. ADH_SF_Zn-type.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Z311-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MLVSRRLTGA RARAPLLASL LEAWCRQGRT TSSYSAFSEP SHVRALVYGN
60 70 80 90 100
HGDPAKVIQL KNLELTAVEG SDVHVKMLAA PINPSDINMI QGNYGLLPKL
110 120 130 140 150
PAVGGNEGVG QVIAVGSSVS GLKPGDWVIP ANAGLGTWRT EAVFSEEALI
160 170 180 190 200
GVPKDIPLQS AATLGVNPCT AYRMLVDFEQ LQPGDSVIQN ASNSGVGQAV
210 220 230 240 250
IQIASALGLK TINVIRDRPD IKKLTDRLKD LGADYVLTEE ELRMPETKNI
260 270 280 290 300
FKDLPLPRLA LNCVGGKSST ELLRHLAPGG TMVTYGGMAK QPVTASVSML
310 320 330 340 350
IFKDLKLRGF WLSQWKKNHS PDEFKELILI LCNLIRQGQL TAPAWSGIPL
360 370
QDYQQALEAS MKPFVSLKQI LTM
Length:373
Mass (Da):40,327
Last modified:April 30, 1999 - v1
Checksum:i94D5A057D666320D
GO
Isoform 2 (identifier: Q9Z311-2) [UniParc]FASTAAdd to basket

Also known as: cMECR

The sequence of this isoform differs from the canonical sequence as follows:
     1-76: Missing.

Show »
Length:297
Mass (Da):32,056
Checksum:i729F72296E069761
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 7676Missing in isoform 2. VSP_057305Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015724 mRNA. Translation: BAA34804.1.
AABR06040190 Genomic DNA. No translation available.
AABR06040191 Genomic DNA. No translation available.
RefSeqiNP_058905.1. NM_017209.1. [Q9Z311-1]
UniGeneiRn.15375.

Genome annotation databases

GeneIDi29470.
KEGGirno:29470.
UCSCiRGD:3208. rat. [Q9Z311-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB015724 mRNA. Translation: BAA34804.1.
AABR06040190 Genomic DNA. No translation available.
AABR06040191 Genomic DNA. No translation available.
RefSeqiNP_058905.1. NM_017209.1. [Q9Z311-1]
UniGeneiRn.15375.

3D structure databases

ProteinModelPortaliQ9Z311.
SMRiQ9Z311. Positions 38-373.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000031375.

PTM databases

PhosphoSiteiQ9Z311.

Proteomic databases

PaxDbiQ9Z311.
PRIDEiQ9Z311.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29470.
KEGGirno:29470.
UCSCiRGD:3208. rat. [Q9Z311-1]

Organism-specific databases

CTDi51102.
RGDi3208. Mecr.

Phylogenomic databases

eggNOGiCOG0604.
HOGENOMiHOG000294683.
HOVERGENiHBG052446.
InParanoidiQ9Z311.
KOiK07512.
OMAiCRAWGIN.
OrthoDBiEOG78M024.
PhylomeDBiQ9Z311.

Miscellaneous databases

NextBioi35576277.
PROiQ9Z311.

Gene expression databases

GenevestigatoriQ9Z311.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiIPR013149. ADH_C.
IPR013154. ADH_GroES-like.
IPR002085. ADH_SF_Zn-type.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR11695. PTHR11695. 1 hit.
PfamiPF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
[Graphical view]
SUPFAMiSSF50129. SSF50129. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nuclear receptor binding factor-1 (NRBF-1), a protein interacting with a wide spectrum of nuclear hormone receptors."
    Masuda N., Yasumo H., Furusawa T., Tsukamoto T., Sadano H., Osumi T.
    Gene 221:225-233(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Genome sequence of the Brown Norway rat yields insights into mammalian evolution."
    Gibbs R.A., Weinstock G.M., Metzker M.L., Muzny D.M., Sodergren E.J., Scherer S., Scott G., Steffen D., Worley K.C., Burch P.E., Okwuonu G., Hines S., Lewis L., Deramo C., Delgado O., Dugan-Rocha S., Miner G., Morgan M.
    , Hawes A., Gill R., Holt R.A., Adams M.D., Amanatides P.G., Baden-Tillson H., Barnstead M., Chin S., Evans C.A., Ferriera S., Fosler C., Glodek A., Gu Z., Jennings D., Kraft C.L., Nguyen T., Pfannkoch C.M., Sitter C., Sutton G.G., Venter J.C., Woodage T., Smith D., Lee H.-M., Gustafson E., Cahill P., Kana A., Doucette-Stamm L., Weinstock K., Fechtel K., Weiss R.B., Dunn D.M., Green E.D., Blakesley R.W., Bouffard G.G., De Jong P.J., Osoegawa K., Zhu B., Marra M., Schein J., Bosdet I., Fjell C., Jones S., Krzywinski M., Mathewson C., Siddiqui A., Wye N., McPherson J., Zhao S., Fraser C.M., Shetty J., Shatsman S., Geer K., Chen Y., Abramzon S., Nierman W.C., Havlak P.H., Chen R., Durbin K.J., Egan A., Ren Y., Song X.-Z., Li B., Liu Y., Qin X., Cawley S., Cooney A.J., D'Souza L.M., Martin K., Wu J.Q., Gonzalez-Garay M.L., Jackson A.R., Kalafus K.J., McLeod M.P., Milosavljevic A., Virk D., Volkov A., Wheeler D.A., Zhang Z., Bailey J.A., Eichler E.E., Tuzun E., Birney E., Mongin E., Ureta-Vidal A., Woodwark C., Zdobnov E., Bork P., Suyama M., Torrents D., Alexandersson M., Trask B.J., Young J.M., Huang H., Wang H., Xing H., Daniels S., Gietzen D., Schmidt J., Stevens K., Vitt U., Wingrove J., Camara F., Mar Alba M., Abril J.F., Guigo R., Smit A., Dubchak I., Rubin E.M., Couronne O., Poliakov A., Huebner N., Ganten D., Goesele C., Hummel O., Kreitler T., Lee Y.-A., Monti J., Schulz H., Zimdahl H., Himmelbauer H., Lehrach H., Jacob H.J., Bromberg S., Gullings-Handley J., Jensen-Seaman M.I., Kwitek A.E., Lazar J., Pasko D., Tonellato P.J., Twigger S., Ponting C.P., Duarte J.M., Rice S., Goodstadt L., Beatson S.A., Emes R.D., Winter E.E., Webber C., Brandt P., Nyakatura G., Adetobi M., Chiaromonte F., Elnitski L., Eswara P., Hardison R.C., Hou M., Kolbe D., Makova K., Miller W., Nekrutenko A., Riemer C., Schwartz S., Taylor J., Yang S., Zhang Y., Lindpaintner K., Andrews T.D., Caccamo M., Clamp M., Clarke L., Curwen V., Durbin R.M., Eyras E., Searle S.M., Cooper G.M., Batzoglou S., Brudno M., Sidow A., Stone E.A., Payseur B.A., Bourque G., Lopez-Otin C., Puente X.S., Chakrabarti K., Chatterji S., Dewey C., Pachter L., Bray N., Yap V.B., Caspi A., Tesler G., Pevzner P.A., Haussler D., Roskin K.M., Baertsch R., Clawson H., Furey T.S., Hinrichs A.S., Karolchik D., Kent W.J., Rosenbloom K.R., Trumbower H., Weirauch M., Cooper D.N., Stenson P.D., Ma B., Brent M., Arumugam M., Shteynberg D., Copley R.R., Taylor M.S., Riethman H., Mudunuri U., Peterson J., Guyer M., Felsenfeld A., Old S., Mockrin S., Collins F.S.
    Nature 428:493-521(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Brown Norway.
  3. "A novel cytosolic isoform of mitochondrial Trans-2-Enoyl-CoA reductase enhances peroxisome proliferator-activated receptor alpha activity."
    Kim D.G., Yoo J.C., Kim E., Lee Y.S., Yarishkin O.V., Lee da Y., Lee K.H., Hong S.G., Hwang E.M., Park J.Y.
    Endocrinol. Metab. 29:185-194(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), SUBCELLULAR LOCATION (ISOFORM 2), INTERACTION WITH PPARA (ISOFORM 2).
  4. "Characterization of 2-enoyl thioester reductase from mammals: an ortholog of Ybr026p/Mrf1'p of the yeast mitochondrial fatty acid synthesis type II."
    Miinalainen I.J., Chen Z.-J., Torkko J.M., Pirilae P.L., Sormunen R.T., Bergmann U., Qin Y.-M., Hiltunen J.K.
    J. Biol. Chem. 278:20154-20161(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION (ISOFORM 1).

Entry informationi

Entry nameiMECR_RAT
AccessioniPrimary (citable) accession number: Q9Z311
Secondary accession number(s): F1LPY7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 25, 2005
Last sequence update: April 30, 1999
Last modified: March 31, 2015
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Was originally (PubMed:9795230) thought to be a nuclear protein that interact with nuclear receptor. However, it was shown later to be mitochondrial (PubMed:12654921), a function related to nuclear receptors being unsure.2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.