Reviewed, UniProtKB/Swiss-Prot Q9Z311 (MECR_RAT)

Names and origin

Protein names

Recommended name:
    Trans-2-enoyl-CoA reductase, mitochondrial
    EC=1.3.1.38
Alternative name(s):
    Nuclear receptor-binding factor 1
      Short name=NRBF-1

Gene names

Name:	Mecr
Synonyms:	Nrbf1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the reduction of trans-2-enoyl-CoA to acyl-CoA with chain length from C6 to C16 in an NADPH-dependent manner with preference to medium chain length substrate. May have a role in the mitochondrial synthesis of fatty acids By similarity.
Catalytic activity	Acyl-CoA + NADP+ = trans-2,3-dehydroacyl-CoA + NADPH.
Subunit structure	Homodimer By similarity.
Subcellular location	Mitochondrion. Ref.2
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily.
Caution	Was originally (Ref.1) thought to be a nuclear protein that interact with nuclear receptor. However, it was shown later to be mitochondrial (Ref.2), a function related to nuclear receptors being unsure.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Ref.2 Inferred from direct assay. Source: UniProtKB
Molecular function	receptor binding Ref.1 Inferred from direct assay. Source: RGD trans-2-enoyl-CoA reductase (NADPH) activity Ref.2 Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 53	53	Mitochondrion Potential
	Chain	54 – 373	320	Trans-2-enoyl-CoA reductase, mitochondrial		PRO_0000000890

Sequences

	Sequence		Length	Mass (Da)
	Q9Z311-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 94D5A057D666320D	FASTA	373	40,327
	10 20 30 40 50 60 MLVSRRLTGA RARAPLLASL LEAWCRQGRT TSSYSAFSEP SHVRALVYGN HGDPAKVIQL 70 80 90 100 110 120 KNLELTAVEG SDVHVKMLAA PINPSDINMI QGNYGLLPKL PAVGGNEGVG QVIAVGSSVS 130 140 150 160 170 180 GLKPGDWVIP ANAGLGTWRT EAVFSEEALI GVPKDIPLQS AATLGVNPCT AYRMLVDFEQ 190 200 210 220 230 240 LQPGDSVIQN ASNSGVGQAV IQIASALGLK TINVIRDRPD IKKLTDRLKD LGADYVLTEE 250 260 270 280 290 300 ELRMPETKNI FKDLPLPRLA LNCVGGKSST ELLRHLAPGG TMVTYGGMAK QPVTASVSML 310 320 330 340 350 360 IFKDLKLRGF WLSQWKKNHS PDEFKELILI LCNLIRQGQL TAPAWSGIPL QDYQQALEAS 370 MKPFVSLKQI LTM

References

[1]	"Nuclear receptor binding factor-1 (NRBF-1), a protein interacting with a wide spectrum of nuclear hormone receptors." Masuda N., Yasumo H., Furusawa T., Tsukamoto T., Sadano H., Osumi T. Gene 221:225-233(1998) [PubMed: 9795230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Characterization of 2-enoyl thioester reductase from mammals: an ortholog of Ybr026p/Mrf1'p of the yeast mitochondrial fatty acid synthesis type II." Miinalainen I.J., Chen Z.-J., Torkko J.M., Pirilae P.L., Sormunen R.T., Bergmann U., Qin Y.-M., Hiltunen J.K. J. Biol. Chem. 278:20154-20161(2003) [PubMed: 12654921] [Abstract] Cited for: SUBCELLULAR LOCATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB015724 mRNA. Translation: BAA34804.1.
IPI	IPI00210183.
RefSeq	NP_058905.1.
UniGene	Rn.15375
3D structure databases
HSSP	HSSP built from PDB template 1GU7 based on UniProtKB Q8WZM3.
SMR	Q9Z311. Positions 38-373.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9Z311.
Genome annotation databases
Ensembl	ENSRNOT00000034057; ENSRNOP00000031375; ENSRNOG00000028047; Rattus norvegicus. [Genome view]
GeneID	29470.
KEGG	rno:29470.
UCSC	NM_017209. rat.
Organism-specific databases
CTD	29470.
RGD	3208. Mecr.
Phylogenomic databases
HOVERGEN	Q9Z311.
Enzyme and pathway databases
BRENDA	1.3.1.38. 248.
Gene expression databases
ArrayExpress	Q9Z311.
Genevestigator	Q9Z311.
GermOnline	ENSRNOG00000028047. Rattus norvegicus.
Family and domain databases
InterPro	IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn. IPR013149. ADH_Zn-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11695. ADH_Sf_Zn. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	609292.

Entry information

Entry name

MECR_RAT

Accession

Primary (citable) accession number: Q9Z311

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	May 1, 1999
Last modified:	October 13, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)