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Protein

Inward rectifier potassium channel 16

Gene

Kcnj16

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. KCNJ16 may be involved in the regulation of fluid and pH balance. In the kidney, together with KCNJ10, mediates basolateral K+ recycling in distal tubules; this process is critical for Na+ reabsorption at the tubules.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei161 – 1611Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesiumBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Voltage-gated channel

Keywords - Biological processi

Ion transport, Potassium transport, Transport

Keywords - Ligandi

Potassium

Enzyme and pathway databases

ReactomeiR-MMU-1296041. Activation of G protein gated Potassium channels.
R-MMU-1296067. Potassium transport channels.
R-MMU-997272. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Names & Taxonomyi

Protein namesi
Recommended name:
Inward rectifier potassium channel 16
Alternative name(s):
Inward rectifier K(+) channel Kir5.1
Potassium channel, inwardly rectifying subfamily J member 16
Gene namesi
Name:Kcnj16
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1314842. Kcnj16.

Subcellular locationi

  • Membrane By similarity; Multi-pass membrane protein By similarity
  • Basolateral cell membrane By similarity

  • Note: In kidney distal convoluted tubules, located in the basolateral membrane in the presence of KCNJ10.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7070CytoplasmicBy similarityAdd
BLAST
Transmembranei71 – 9525Helical; Name=M1By similarityAdd
BLAST
Topological domaini96 – 11722ExtracellularBy similarityAdd
BLAST
Intramembranei118 – 12912Helical; Pore-forming; Name=H5By similarityAdd
BLAST
Intramembranei130 – 1367Pore-formingBy similarity
Topological domaini137 – 1459ExtracellularBy similarity
Transmembranei146 – 16722Helical; Name=M2By similarityAdd
BLAST
Topological domaini168 – 419252CytoplasmicBy similarityAdd
BLAST

GO - Cellular componenti

  • basolateral plasma membrane Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 419419Inward rectifier potassium channel 16PRO_0000154976Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei358 – 3581PhosphoserineCombined sources
Modified residuei374 – 3741PhosphoserineCombined sources
Modified residuei376 – 3761PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9Z307.
PaxDbiQ9Z307.
PRIDEiQ9Z307.

PTM databases

iPTMnetiQ9Z307.
PhosphoSiteiQ9Z307.

Expressioni

Gene expression databases

BgeeiQ9Z307.
ExpressionAtlasiQ9Z307. baseline and differential.
GenevisibleiQ9Z307. MM.

Interactioni

Subunit structurei

Heterodimer with Kir4.1/KCNJ10; this interaction is required for KCNJ16 localization to the basolateral membrane in kidney cells. As a heterodimer with KCNJ10, may interact with MAGI1; this interaction may facilitate KCNJ10/KCNJ16 potassium channel expression at the basolateral membrane in kidney cells. May form heterodimers with Kir2.1/KCNJ2.By similarity

Protein-protein interaction databases

IntActiQ9Z307. 1 interaction.
STRINGi10090.ENSMUSP00000102246.

Structurei

3D structure databases

ProteinModelPortaliQ9Z307.
SMRiQ9Z307. Positions 33-344.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi131 – 1366Selectivity filterBy similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3827. Eukaryota.
ENOG410XQ62. LUCA.
GeneTreeiENSGT00760000118842.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiQ9Z307.
KOiK05009.
OMAiSIFWLIA.
OrthoDBiEOG7XPZ5K.
TreeFamiTF313676.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR008061. K_chnl_inward-rec_Kir5.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF24. PTHR11767:SF24. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01678. KIR5CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9Z307-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYYGSSYRI VNVDSKYPGY PPEHAIAEKR RARRRLLHKD GSCNVYFKHI
60 70 80 90 100
FGEWGSYMVD IFTTLVDTKW RHMFVIFSLS YILSWLIFGS IFWLIAFHHG
110 120 130 140 150
DLLSDPDITP CVDNVHSFTA AFLFSLETQT TIGYGYRCVT EECSVAVLTV
160 170 180 190 200
ILQSILSCII NTFIIGAALA KMATARKRAQ TIRFSYFALI GMRDGKLCLM
210 220 230 240 250
WRIGDFRPNH VVEGTVRAQL LRYSEDSEGR MTMAFKDLKL VNDQIILVTP
260 270 280 290 300
VTIVHEIDHE SPLYALDRKA VAKDNFEILV TFIYTGDSTG TSHQSRSSYI
310 320 330 340 350
PREILWGHRF HDVLEVKRKY YKVNCLQFEG SVEVYAPFCS AKQLDWKDQQ
360 370 380 390 400
LNNLEKTSPA RGSCNSDTNT RRRSFSAVAV VSSCENPEET VLSPQDECKE
410
MPYQKALLTL NRISMESQM
Length:419
Mass (Da):48,023
Last modified:July 27, 2011 - v2
Checksum:i533D95129D7EB7AF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti75 – 751V → I in BAA34723 (PubMed:9806850).Curated
Sequence conflicti197 – 1971L → P in BAA34723 (PubMed:9806850).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016197 mRNA. Translation: BAA34723.1.
AY377989 mRNA. Translation: AAQ88167.1.
AK032192 mRNA. Translation: BAC27749.1.
AK045447 mRNA. Translation: BAC32374.1.
AK078905 mRNA. Translation: BAC37450.1.
AK140249 mRNA. Translation: BAE24297.1.
AL592422 Genomic DNA. Translation: CAM17827.1.
CH466558 Genomic DNA. Translation: EDL34389.1.
CH466558 Genomic DNA. Translation: EDL34390.1.
CH466558 Genomic DNA. Translation: EDL34391.1.
CH466558 Genomic DNA. Translation: EDL34392.1.
CCDSiCCDS25593.1.
RefSeqiNP_001239136.1. NM_001252207.1.
NP_001239137.1. NM_001252208.1.
NP_001239138.1. NM_001252209.2.
NP_001239139.1. NM_001252210.2.
NP_034734.3. NM_010604.3.
UniGeneiMm.30176.

Genome annotation databases

EnsembliENSMUST00000106635; ENSMUSP00000102246; ENSMUSG00000051497.
ENSMUST00000106636; ENSMUSP00000102247; ENSMUSG00000051497.
ENSMUST00000178798; ENSMUSP00000137414; ENSMUSG00000051497.
ENSMUST00000180023; ENSMUSP00000136382; ENSMUSG00000051497.
GeneIDi16517.
KEGGimmu:16517.
UCSCiuc007mdu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016197 mRNA. Translation: BAA34723.1.
AY377989 mRNA. Translation: AAQ88167.1.
AK032192 mRNA. Translation: BAC27749.1.
AK045447 mRNA. Translation: BAC32374.1.
AK078905 mRNA. Translation: BAC37450.1.
AK140249 mRNA. Translation: BAE24297.1.
AL592422 Genomic DNA. Translation: CAM17827.1.
CH466558 Genomic DNA. Translation: EDL34389.1.
CH466558 Genomic DNA. Translation: EDL34390.1.
CH466558 Genomic DNA. Translation: EDL34391.1.
CH466558 Genomic DNA. Translation: EDL34392.1.
CCDSiCCDS25593.1.
RefSeqiNP_001239136.1. NM_001252207.1.
NP_001239137.1. NM_001252208.1.
NP_001239138.1. NM_001252209.2.
NP_001239139.1. NM_001252210.2.
NP_034734.3. NM_010604.3.
UniGeneiMm.30176.

3D structure databases

ProteinModelPortaliQ9Z307.
SMRiQ9Z307. Positions 33-344.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9Z307. 1 interaction.
STRINGi10090.ENSMUSP00000102246.

PTM databases

iPTMnetiQ9Z307.
PhosphoSiteiQ9Z307.

Proteomic databases

MaxQBiQ9Z307.
PaxDbiQ9Z307.
PRIDEiQ9Z307.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000106635; ENSMUSP00000102246; ENSMUSG00000051497.
ENSMUST00000106636; ENSMUSP00000102247; ENSMUSG00000051497.
ENSMUST00000178798; ENSMUSP00000137414; ENSMUSG00000051497.
ENSMUST00000180023; ENSMUSP00000136382; ENSMUSG00000051497.
GeneIDi16517.
KEGGimmu:16517.
UCSCiuc007mdu.1. mouse.

Organism-specific databases

CTDi3773.
MGIiMGI:1314842. Kcnj16.

Phylogenomic databases

eggNOGiKOG3827. Eukaryota.
ENOG410XQ62. LUCA.
GeneTreeiENSGT00760000118842.
HOGENOMiHOG000237325.
HOVERGENiHBG006178.
InParanoidiQ9Z307.
KOiK05009.
OMAiSIFWLIA.
OrthoDBiEOG7XPZ5K.
TreeFamiTF313676.

Enzyme and pathway databases

ReactomeiR-MMU-1296041. Activation of G protein gated Potassium channels.
R-MMU-1296067. Potassium transport channels.
R-MMU-997272. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Miscellaneous databases

ChiTaRSiKcnj16. mouse.
NextBioi289881.
PROiQ9Z307.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Z307.
ExpressionAtlasiQ9Z307. baseline and differential.
GenevisibleiQ9Z307. MM.

Family and domain databases

Gene3Di2.60.40.1400. 1 hit.
InterProiIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR008061. K_chnl_inward-rec_Kir5.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERiPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF24. PTHR11767:SF24. 1 hit.
PfamiPF01007. IRK. 1 hit.
[Graphical view]
PIRSFiPIRSF005465. GIRK_kir. 1 hit.
PRINTSiPR01678. KIR5CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMiSSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Assignment of mouse inwardly rectifying potassium channel Kcnj16 to the distal region of mouse chromosome 11."
    Mouri T., Kittaka N., Horio Y., Copeland N.G., Gilbert D.J., Jenkins N.A., Kurachi Y.
    Genomics 54:181-182(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  2. "Interaction of inner ear-specific Kir channels."
    Nie L., Feng W.H., Vazquez A.E., Yamoah E.N.
    Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
    Tissue: Cochlea.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Cecum, Corpora quadrigemina and Olfactory bulb.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-358; SER-374 AND SER-376, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney.

Entry informationi

Entry nameiKCJ16_MOUSE
AccessioniPrimary (citable) accession number: Q9Z307
Secondary accession number(s): Q8BH37
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: July 27, 2011
Last modified: May 11, 2016
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.