Q9Z307 (IRK16_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inward rectifier potassium channel 16

Alternative name(s):
Inward rectifier K(+) channel Kir5.1
Potassium channel, inwardly rectifying subfamily J member 16

Gene names

Name:

Kcnj16

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	419 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. KCNJ16 may be involved in the regulation of fluid and pH balance By similarity.
Subunit structure	Seems to form heterodimer with Kir4.1/KCNJ10 or Kir2.1/KCNJ2 By similarity.
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ16 subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
Molecular function	Ionic channel Voltage-gated channel
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay. Source: MGI
Molecular function	inward rectifier potassium channel activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 419	419	Inward rectifier potassium channel 16		PRO_0000154976
Regions
	Topological domain	1 – 70	70	Cytoplasmic By similarity
	Transmembrane	71 – 95	25	Helical; Name=M1; By similarity
	Topological domain	96 – 117	22	Extracellular By similarity
	Intramembrane	118 – 129	12	Helical; Pore-forming; Name=H5; By similarity
	Intramembrane	130 – 136	7	Pore-forming; By similarity
	Topological domain	137 – 145	9	Extracellular By similarity
	Transmembrane	146 – 167	22	Helical; Name=M2; By similarity
	Topological domain	168 – 419	252	Cytoplasmic By similarity
	Motif	131 – 136	6	Selectivity filter By similarity
Sites
	Site	161	1	Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium By similarity
Experimental info
	Sequence conflict	75	1	V → I in BAA34723. Ref.1
	Sequence conflict	197	1	L → P in BAA34723. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9Z307 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 533D95129D7EB7AF	FASTA	419	48,023
	10 20 30 40 50 60 MSYYGSSYRI VNVDSKYPGY PPEHAIAEKR RARRRLLHKD GSCNVYFKHI FGEWGSYMVD 70 80 90 100 110 120 IFTTLVDTKW RHMFVIFSLS YILSWLIFGS IFWLIAFHHG DLLSDPDITP CVDNVHSFTA 130 140 150 160 170 180 AFLFSLETQT TIGYGYRCVT EECSVAVLTV ILQSILSCII NTFIIGAALA KMATARKRAQ 190 200 210 220 230 240 TIRFSYFALI GMRDGKLCLM WRIGDFRPNH VVEGTVRAQL LRYSEDSEGR MTMAFKDLKL 250 260 270 280 290 300 VNDQIILVTP VTIVHEIDHE SPLYALDRKA VAKDNFEILV TFIYTGDSTG TSHQSRSSYI 310 320 330 340 350 360 PREILWGHRF HDVLEVKRKY YKVNCLQFEG SVEVYAPFCS AKQLDWKDQQ LNNLEKTSPA 370 380 390 400 410 RGSCNSDTNT RRRSFSAVAV VSSCENPEET VLSPQDECKE MPYQKALLTL NRISMESQM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Assignment of mouse inwardly rectifying potassium channel Kcnj16 to the distal region of mouse chromosome 11." Mouri T., Kittaka N., Horio Y., Copeland N.G., Gilbert D.J., Jenkins N.A., Kurachi Y. Genomics 54:181-182(1998) [PubMed: 9806850] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Brain.
[2]	"Interaction of inner ear-specific Kir channels." Nie L., Feng W.H., Vazquez A.E., Yamoah E.N. Submitted (SEP-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6. Tissue: Cochlea.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Cecum, Corpora quadrigemina and Olfactory bulb.
[4]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6.
[5]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB016197 mRNA. Translation: BAA34723.1. AY377989 mRNA. Translation: AAQ88167.1. AK032192 mRNA. Translation: BAC27749.1. AK045447 mRNA. Translation: BAC32374.1. AK078905 mRNA. Translation: BAC37450.1. AK140249 mRNA. Translation: BAE24297.1. AL592422 Genomic DNA. Translation: CAM17827.1. CH466558 Genomic DNA. Translation: EDL34389.1. CH466558 Genomic DNA. Translation: EDL34390.1. CH466558 Genomic DNA. Translation: EDL34391.1. CH466558 Genomic DNA. Translation: EDL34392.1.
IPI	IPI00131294.
RefSeq	NP_001239136.1. NM_001252207.1. NP_001239137.1. NM_001252208.1. NP_001239138.1. NM_001252209.1. NP_001239139.1. NM_001252210.1. NP_034734.3. NM_010604.3.
UniGene	Mm.30176.
3D structure databases
ProteinModelPortal	Q9Z307.
SMR	Q9Z307. Positions 33-353.
ModBase	Search...
Protein-protein interaction databases
IntAct	Q9Z307. 1 interaction.
STRING	Q9Z307.
PTM databases
PhosphoSite	Q9Z307.
Proteomic databases
PRIDE	Q9Z307.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000106635; ENSMUSP00000102246; ENSMUSG00000051497. ENSMUST00000106636; ENSMUSP00000102247; ENSMUSG00000051497.
GeneID	16517.
KEGG	mmu:16517.
Organism-specific databases
CTD	3773.
MGI	MGI:1314842. Kcnj16.
Phylogenomic databases
GeneTree	ENSGT00560000076991.
HOGENOM	HBG716702.
HOVERGEN	HBG006178.
InParanoid	Q9Z307.
OrthoDB	EOG4GB763.
Gene expression databases
ArrayExpress	Q9Z307.
Bgee	Q9Z307.
Genevestigator	Q9Z307.
GermOnline	ENSMUSG00000051497. Mus musculus.
Family and domain databases
InterPro	IPR014756. Ig_E-set. IPR016449. K_chnl_inward-rec_Kir. IPR001838. K_chnl_inward-rec_Kir-like. IPR008061. K_chnl_inward-rec_Kir5. IPR013521. K_chnl_inward-rec_Kir_Cr2. IPR013518. K_chnl_inward-rec_Kir_cyto. [Graphical view]
Gene3D	G3DSA:2.60.40.1400. IR_K+channel_cytopl. 1 hit.
KO	K05009.
PANTHER	PTHR11767. K+channel_IR. 1 hit. PTHR11767:SF24. KIR50_channel. 1 hit.
Pfam	PF01007. IRK. 1 hit. [Graphical view]
PIRSF	PIRSF005465. GIRK_kir. 1 hit.
PRINTS	PR01678. KIR5CHANNEL. PR01320. KIRCHANNEL.
SUPFAM	SSF81296. Ig_E-set. 1 hit.
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

IRK16_MOUSE

Accession

Primary (citable) accession number: Q9Z307
Secondary accession number(s): Q8BH37

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	July 27, 2011
Last modified:	January 25, 2012
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program