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Q9Z301 (PER2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 98. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Period circadian protein homolog 2

Short name=rPER2
Alternative name(s):
Circadian clock protein PERIOD 2
Gene names
Name:Per2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length1257 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the circadian clock mechanism which is essential for generating circadian rhythms. Negative element in the circadian transcriptional loop. Influences clock function by interacting with other circadian regulatory proteins and transporting them to the nucleus. Negatively regulates CLOCK|NPAS2-BMAL1|BMAL2-induced transactivation By similarity.

Subunit structure

Homodimer. Component of the circadian core oscillator, which includes the CRY proteins, CLOCK or NPAS2, BMAL1 or BMAL2, CSNK1D and/or CSNK1E, TIMELESS, and the PER proteins. Interacts directly with PER1 and PER3, and through a C-terminal domain, with CRY1 and CRY2. Interaction with CSNK1D or CSNK1E promotes nuclear location of PER proteins. Interacts, via its second PAS domain, with TIMELESS in vitro. Interacts with NFIL3 By similarity. Interacts with PML By similarity. Interacts with CIART By similarity. Ref.2 Ref.3

Subcellular location

Nucleus. Cytoplasm. Cytoplasmperinuclear region By similarity. Note: Mainly nuclear. Nucleocytoplasmic shuttling is effected by interaction with other circadian core oscillator proteins and/or by phosphorylation. Retention of PER1 in the cytoplasm occurs through PER1-PER2 heterodimer formation or by interaction with CSNK1E and/or phosphorylation which appears to mask the PER nuclear localization signal. Also translocated to the nucleus by CRY1 or CRY2. PML regulates its nuclear localization By similarity. Ref.3

Tissue specificity

Expressed in all tissues examined including eye, brain, heart, lung, spleen, liver, pancreas and kidney. In the CNS, highly expressed in the SCN, internal granular layer of granular cells of the olfactory bulb, tuberculum olfactorium, piriform cortex, gyrus dentatus of the hippocampus, cerebellum, pars tuberalis/median eminence, and pituitary, and moderately in the tenia tecta, caudate putamen, accumbens nucleus, superior and inferior colliculus and pineal gland. Ref.1 Ref.4 Ref.5

Induction

In eye, brain, heart, lung, spleen, liver, pancreas and kidney, expression exhibits a circadian rhythm in the presence of light/dark cycles. Ref.1 Ref.5

Post-translational modification

Phosphorylated by CSNK1E and CSNK1D. Phosphorylation results in PER2 protein degradation By similarity.

Sequence similarities

Contains 1 PAC (PAS-associated C-terminal) domain.

Contains 2 PAS (PER-ARNT-SIM) domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12571257Period circadian protein homolog 2
PRO_0000162632

Regions

Domain179 – 24668PAS 1
Domain319 – 38567PAS 2
Domain393 – 43644PAC
Region555 – 763209CSNK1E binding domain
Region1157 – 1257101CRY binding domain
Motif457 – 46913Nuclear export signal By similarity
Motif778 – 79417Nuclear localization signal Ref.3
Compositional bias831 – 961131Pro-rich
Compositional bias1033 – 111179Ser-rich

Amino acid modifications

Modified residue5251Phosphoserine By similarity
Modified residue5281Phosphoserine By similarity
Modified residue5311Phosphoserine By similarity
Modified residue5381Phosphoserine By similarity
Modified residue5441Phosphoserine By similarity
Modified residue5541Phosphothreonine By similarity
Modified residue6591Phosphoserine By similarity
Modified residue6931Phosphoserine By similarity
Modified residue6971Phosphoserine By similarity
Modified residue7061Phosphoserine By similarity
Modified residue7581Phosphoserine By similarity
Modified residue7631Phosphoserine By similarity
Modified residue8581Phosphothreonine By similarity
Modified residue9391Phosphoserine By similarity
Modified residue9641Phosphothreonine By similarity
Modified residue9711Phosphoserine By similarity
Modified residue11261Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
Q9Z301 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: A772E3C453E63CED

FASTA1,257136,028
        10         20         30         40         50         60 
MNGYVDFSPS PTSPTQEPGE PQPTQAVLQE DVDMSSGSSG NENCSTGRDS QGSDCDDSGK 

        70         80         90        100        110        120 
ELRMLVESSN THPSPDDTFR LMMTEAEHNP STSGCSSEQS AKADAHKELI RTLRELKVHL 

       130        140        150        160        170        180 
PADKKAKGKA STLATLKYAL RSVKQVKANE EYYQLLMSSE SQPCSVDVPS YTMEQVEGIT 

       190        200        210        220        230        240 
SEYIVKNSDM FAVAVSLVSG KILYISNQVA PIFHCKKDAF SDAKFVEFLA PHDVSVFHSY 

       250        260        270        280        290        300 
TTPYKLPPWS VSSGLDSFTQ ECMEEKSFFC RVSVGKHHEN EIRYQPFRMT PYLVKVQEQK 

       310        320        330        340        350        360 
GAASQLCCLL LAERVHSGYE APRIPPEKRI FTTTHTPNCL FQDVDERAVP LLGYLPQDLI 

       370        380        390        400        410        420 
ETPVLVQLHP SDRPLMLAIH KKILQASGQP FDYSPIRFRT RNGEYITLDT SWSSFINPWS 

       430        440        450        460        470        480 
RKISFIIGRH KVRVGPLNED VFAASPCPEE KTPHPSVQEL TEQIHRLLMQ PVPHSGSSGY 

       490        500        510        520        530        540 
GSLGSNGSHE HLMSQTSSSD SNGQEESHWR RSGIFKTSGK SQSKSHFSPE SGGQKEASVA 

       550        560        570        580        590        600 
EMQSSPPAQV RSVTTMERDS SGASLPKASF PEELTYKSQP PCSYQQISCL DSVIRYLESC 

       610        620        630        640        650        660 
NEAATLKRKC EFPANIPSRK ATVSPGLHSG EAARSSKVTS HTEVSAHLSS LALPGKAESV 

       670        680        690        700        710        720 
VSLTSQCSYS STIVHVGDKK PQPELETVED VASGPESQDD AAGGLSQEKG SLQKLGLTKE 

       730        740        750        760        770        780 
VLAAHTQREE QGFLQRFREV SRLGALQAHC QNYLQERSRA PASDRGLRNA SGIESSWKKT 

       790        800        810        820        830        840 
GKNRKLKSKR VKTRDSSEST GSGGPVSHRP PLVGLNATAW SPSDTSQSSC PSAPFPAPVP 

       850        860        870        880        890        900 
AYPLPVFPAP GIVSTPGTVV APPAAAHTGF TMPVVPMGTQ PEFAVQPLPF AAPLAPVMAF 

       910        920        930        940        950        960 
MLPSYPFPPA TPNLPQAFFP SQPHFPAHPT LASEITPASQ AEFPSRTSML RQPCACPVTP 

       970        980        990       1000       1010       1020 
PAGTVALGRA SPPLFQSRGS SPLQLNLLQL EEAPESSTGA AGTLGTTGTA ASGLDCTSGA 

      1030       1040       1050       1060       1070       1080 
SRDRQPKAPP TCSEPSDTQN SDAISTSSDL LNLLLGEDLC SATGSALSRS GASATSDSLG 

      1090       1100       1110       1120       1130       1140 
SSSLGCDTSR SGAGSSDTSH TSKYFGSIDS SENNHKAKMI TDTEESEQFI KYVLQDPIWL 

      1150       1160       1170       1180       1190       1200 
LMANTDDNIM MTYQLPSRDL QAVLKEDQEK LKLLQRSQPH FTEGQRRELR EVHPWVHTGG 

      1210       1220       1230       1240       1250 
LPTAIDVTGC VYCESEEKGN LCLPYEEDSP SLGLCDTSEA KEEESGQLAN PRKEAQT 

« Hide

References

[1]"Multitissue circadian expression of rat period homologue (rPer2) mRNA is governed by the mammalian circadian clock, the suprachiasmatic nucleus in the brain."
Sakamoto K., Nagase T., Fukui H., Horikawa K., Okada T., Tanaka H., Sato K., Miyake Y., Ohara O., Kako K., Ishida N.
J. Biol. Chem. 273:27039-27042(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
Strain: Sprague-Dawley.
Tissue: Brain.
[2]"Molecular characterization and nuclear localization of rat timeless-like gene product."
Sakamoto S., Miyazaki K., Fukui H., Oishi K., Hayasaka N., Okada M., Kamakura M., Taniguchi T., Nagai K., Ishida N.
Biochem. Biophys. Res. Commun. 279:131-138(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TIMELESS.
[3]"Nuclear entry mechanism of rat PER2 (rPER2): role of rPER2 in nuclear localization of CRY protein."
Miyazaki K., Mesaki M., Ishida N.
Mol. Cell. Biol. 21:6651-6659(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CRY1, CRY BINDING DOMAIN, NUCLEAR LOCALIZATION SIGNAL, SUBCELLULAR LOCATION.
[4]"Distribution of the rhythm-related genes rPERIOD1, rPERIOD2, and rCLOCK, in the rat brain."
Shieh K.-R.
Neuroscience 118:831-843(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[5]"Indication of circadian oscillations in the rat pancreas."
Muehlbauer E., Wolgast S., Finckh U., Peschke D., Peschke E.
FEBS Lett. 564:91-96(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB016532 mRNA. Translation: BAA34187.1.
PIRT13957.
RefSeqNP_113866.1. NM_031678.1.
XP_006245538.1. XM_006245476.1.
XP_006245539.1. XM_006245477.1.
UniGeneRn.25935.

3D structure databases

ProteinModelPortalQ9Z301.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid248899. 2 interactions.
STRING10116.ENSRNOP00000027507.

Proteomic databases

PaxDbQ9Z301.
PRIDEQ9Z301.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000027506; ENSRNOP00000027507; ENSRNOG00000020254.
GeneID63840.
KEGGrno:63840.

Organism-specific databases

CTD8864.
RGD61945. Per2.

Phylogenomic databases

eggNOGNOG253593.
GeneTreeENSGT00510000046467.
HOGENOMHOG000231111.
HOVERGENHBG008167.
InParanoidQ9Z301.
KOK02633.
OMANATAWSP.
OrthoDBEOG78SQH2.
PhylomeDBQ9Z301.
TreeFamTF318445.

Gene expression databases

GenevestigatorQ9Z301.

Family and domain databases

InterProIPR001610. PAC.
IPR000014. PAS.
IPR013655. PAS_fold_3.
IPR022728. Period_circadian-like_C.
[Graphical view]
PfamPF08447. PAS_3. 1 hit.
PF12114. Period_C. 1 hit.
[Graphical view]
SMARTSM00086. PAC. 1 hit.
SM00091. PAS. 2 hits.
[Graphical view]
SUPFAMSSF55785. SSF55785. 1 hit.
PROSITEPS50112. PAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio612434.
PROQ9Z301.

Entry information

Entry namePER2_RAT
AccessionPrimary (citable) accession number: Q9Z301
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: May 1, 1999
Last modified: April 16, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families