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Protein

Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2

Gene

Gfpt2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins.

Catalytic activityi

L-glutamine + D-fructose 6-phosphate = L-glutamate + D-glucosamine 6-phosphate.By similarity

Pathwayi: UDP-N-acetyl-alpha-D-glucosamine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate.By similarity
Proteins known to be involved in this subpathway in this organism are:
  1. Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2 (Gfpt2), Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 1 (Gfpt1)
This subpathway is part of the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes alpha-D-glucosamine 6-phosphate from D-fructose 6-phosphate, the pathway UDP-N-acetyl-alpha-D-glucosamine biosynthesis and in Nucleotide-sugar biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2For GATase activityBy similarity1
Binding sitei427SubstrateBy similarity1
Binding sitei578SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminotransferase, Transferase

Enzyme and pathway databases

ReactomeiR-MMU-446210 Synthesis of UDP-N-acetyl-glucosamine
UniPathwayiUPA00113; UER00528

Protein family/group databases

MEROPSiC44.975

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2 (EC:2.6.1.16By similarity)
Alternative name(s):
D-fructose-6-phosphate amidotransferase 2
Glutamine:fructose-6-phosphate amidotransferase 2
Short name:
GFAT 2
Short name:
GFAT2
Hexosephosphate aminotransferase 2
Gene namesi
Name:Gfpt2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1338883 Gfpt2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001352842 – 682Glutamine--fructose-6-phosphate aminotransferase [isomerizing] 2Add BLAST681

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei244PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9Z2Z9
PaxDbiQ9Z2Z9
PeptideAtlasiQ9Z2Z9
PRIDEiQ9Z2Z9

PTM databases

iPTMnetiQ9Z2Z9
PhosphoSitePlusiQ9Z2Z9

Expressioni

Gene expression databases

BgeeiENSMUSG00000020363
CleanExiMM_GFPT2
GenevisibleiQ9Z2Z9 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020629

Structurei

3D structure databases

ProteinModelPortaliQ9Z2Z9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 288Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST287
Domaini360 – 499SIS 1PROSITE-ProRule annotationAdd BLAST140
Domaini531 – 672SIS 2PROSITE-ProRule annotationAdd BLAST142

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni377 – 378Substrate-bindingBy similarity2
Regioni422 – 424Substrate-bindingBy similarity3

Keywords - Domaini

Glutamine amidotransferase, Repeat

Phylogenomic databases

eggNOGiKOG1268 Eukaryota
COG0449 LUCA
GeneTreeiENSGT00390000010049
HOGENOMiHOG000258898
HOVERGENiHBG051724
InParanoidiQ9Z2Z9
KOiK00820
OMAiRLDSSTC
OrthoDBiEOG091G02N5
PhylomeDBiQ9Z2Z9
TreeFamiTF300864

Family and domain databases

CDDicd05008 SIS_GlmS_GlmD_1, 1 hit
cd05009 SIS_GlmS_GlmD_2, 1 hit
InterProiView protein in InterPro
IPR017932 GATase_2_dom
IPR035466 GlmS/AgaS_SIS
IPR035490 GlmS/FrlB_SIS
IPR005855 GlmS_trans
IPR029055 Ntn_hydrolases_N
IPR001347 SIS
PfamiView protein in Pfam
PF01380 SIS, 2 hits
SUPFAMiSSF56235 SSF56235, 2 hits
TIGRFAMsiTIGR01135 glmS, 1 hit
PROSITEiView protein in PROSITE
PS51278 GATASE_TYPE_2, 1 hit
PS51464 SIS, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z2Z9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGIFAYMNY RVPKTRKEIF ETLIRGLQRL EYRGYDSAGV AIDGNNHEVK
60 70 80 90 100
ERHIHLVKKR GKVKALDEEL YKQDSMDLKV EFETHFGIAH TRWATHGVPN
110 120 130 140 150
AVNSHPQRSD KDNEFVVIHN GIITNYKDLR KFLESKGYEF ESETDTETIA
160 170 180 190 200
KLIKYVFDNR ETEDITFSTL VERVIQQLEG AFALVFKSIH YPGEAVATRR
210 220 230 240 250
GSPLLIGVRS KYKLSTEQIP VLYPTCNIEN VKNICKTRMK RLDSSTCLHA
260 270 280 290 300
VGDKAVEFFF ASDASAIIEH TNRVIFLEDD DIAAVADGKL SIHRVKRSAT
310 320 330 340 350
DDPSRAIQTL QMELQQIMKG NFSAFMQKEI FEQPESVFNT MRGRVNFETN
360 370 380 390 400
TVLLGGLKDH LKEIRRCRRL IVIGCGTSYH AAVATRQVLE ELTELPVMVE
410 420 430 440 450
LASDFLDRNT PVFRDDVCFF ISQSGETADT LLALRYCKDR GALTVGITNT
460 470 480 490 500
VGSSISRETD CGVHINAGPE IGVASTKAYT SQFISLVMFG LMMSEDRISL
510 520 530 540 550
QNRRQEIIRG LRSLPELIKE VLSLDEKIHD LALELYTQRS LLVMGRGYNY
560 570 580 590 600
ATCLEGALKI KEITYMHSEG ILAGELKHGP LALVDKQMPV IMVIMKDPCF
610 620 630 640 650
AKCQNALQQV TARQGRPIIL CSKDDTESSK FAYKTIELPH TVDCLQGILS
660 670 680
VIPLQLLSFH LAVLRGYDVD FPRNLAKSVT VE
Length:682
Mass (Da):77,009
Last modified:January 23, 2007 - v3
Checksum:iD5F2DA9FA48932B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB016778 Genomic DNA Translation: BAA74727.1
AB016780 mRNA Translation: BAA74729.1
AL606479 Genomic DNA Translation: CAI23939.1
BC031928 mRNA Translation: AAH31928.1
CCDSiCCDS24622.1
RefSeqiNP_038557.1, NM_013529.3
UniGeneiMm.24402

Genome annotation databases

EnsembliENSMUST00000020629; ENSMUSP00000020629; ENSMUSG00000020363
GeneIDi14584
KEGGimmu:14584
UCSCiuc011xud.1 mouse

Similar proteinsi

Entry informationi

Entry nameiGFPT2_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2Z9
Secondary accession number(s): Q5NCL2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 142 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways

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