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Q9Z2Z8 (DHCR7_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
7-dehydrocholesterol reductase

Short name=7-DHC reductase
EC=1.3.1.21
Alternative name(s):
Sterol Delta(7)-reductase
Gene names
Name:Dhcr7
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length471 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Production of cholesterol by reduction of C7-C8 double bond of 7-dehydrocholesterol (7-DHC).

Catalytic activity

Cholesterol + NADP+ = cholesta-5,7-dien-3-beta-ol + NADPH.

Pathway

Steroid biosynthesis; cholesterol biosynthesis.

Subcellular location

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the ERG4/ERG24 family.

Ontologies

Keywords
   Biological processCholesterol biosynthesis
Cholesterol metabolism
Lipid biosynthesis
Lipid metabolism
Steroid biosynthesis
Steroid metabolism
Sterol biosynthesis
Sterol metabolism
   Cellular componentEndoplasmic reticulum
Membrane
   DomainTransmembrane
Transmembrane helix
   LigandNADP
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processblood vessel development

Inferred from electronic annotation. Source: Ensembl

cell differentiation

Inferred from electronic annotation. Source: Ensembl

cholesterol biosynthetic process

Inferred from direct assay Ref.2. Source: RGD

lung development

Inferred from electronic annotation. Source: Ensembl

multicellular organism growth

Inferred from electronic annotation. Source: Ensembl

post-embryonic development

Inferred from electronic annotation. Source: Ensembl

regulation of cell proliferation

Inferred from electronic annotation. Source: Ensembl

regulation of cholesterol biosynthetic process

Inferred from direct assay Ref.2. Source: RGD

   Cellular_componentendoplasmic reticulum membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

integral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

intracellular membrane-bounded organelle

Inferred from direct assay PubMed 10666310PubMed 9831636. Source: RGD

nuclear outer membrane

Inferred from electronic annotation. Source: Ensembl

   Molecular_function7-dehydrocholesterol reductase activity

Inferred from mutant phenotype PubMed 10666310PubMed 9831636. Source: RGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 4714717-dehydrocholesterol reductase
PRO_0000207504

Regions

Transmembrane36 – 5621Helical; Potential
Transmembrane95 – 11521Helical; Potential
Transmembrane144 – 16421Helical; Potential
Transmembrane173 – 19321Helical; Potential
Transmembrane233 – 25321Helical; Potential
Transmembrane262 – 28221Helical; Potential
Transmembrane302 – 32221Helical; Potential
Transmembrane327 – 34721Helical; Potential
Transmembrane416 – 43621Helical; Potential

Sequences

Sequence LengthMass (Da)Tools
Q9Z2Z8 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: EBF0CBC4F4222FDB

FASTA47154,155
        10         20         30         40         50         60 
MASKSQHNAS KAKNHNVKAE SQGQWGRAWE VDWFSLVSVI FLLLFAPFIV YYFIMACDQY 

        70         80         90        100        110        120 
SCSLTAPILD VATGRASLAD IWAKTPPVTA KAAQLYALWV SFQVLLYSWL PDFCHRFLPG 

       130        140        150        160        170        180 
YVGGVQEGAI TPAGIVNKYE VNGLQAWLIT HFLWFVNAYL LSWFSPTIIF DNWIPLLWCA 

       190        200        210        220        230        240 
NILGYAVSTF AMIKGYLFPT SAEDCKFTGN FFYNYMMGIE FNPRIGKWFD FKLFFNGRPG 

       250        260        270        280        290        300 
IVAWTLINLS FAAKQQELYG HVTNSMILVN VLQAIYVLDF FWNETWYLKT IDICHDHFGW 

       310        320        330        340        350        360 
YLGWGDCVWL PYLYTLQGLY LVYHPVQLST PNALGVLLLG LVGYYIFRMT NHQKDLFRRT 

       370        380        390        400        410        420 
DGHCLIWGKK PKAIECSYTS ADGLKHRSKL LVSGFWGVAR HFNYTGDLMG SLAYCLACGG 

       430        440        450        460        470 
GHLLPYFYII YMTILLTHRC LRDEHRCANK YGRDWERYVA AVPYRLLPGI F 

« Hide

References

« Hide 'large scale' references
[1]"Transmembrane configuration of sterol delta 7-reductase as a potential sterol sensing protein."
Nishino H., Ishibashi T.
Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Liver.
[2]"Cholesterol biosynthesis from lanosterol. Molecular cloning, tissue distribution, expression, chromosomal localization, and regulation of rat 7-dehydrocholesterol reductase, a Smith-Lemli-Opitz syndrome-related protein."
Bae S.-H., Lee J.N., Fitzky B.U., Seong J., Paik Y.-K.
J. Biol. Chem. 274:14624-14631(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
[3]"Structure and alternative splicing of the rat 7-dehydrocholesterol reductase gene."
Lee J.-N., Bae S.-H., Paik Y.-K.
Biochim. Biophys. Acta 1576:148-156(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB016800 mRNA. Translation: BAA34306.1.
AF071500 mRNA. Translation: AAD31383.1.
AF272393 mRNA. Translation: AAM45144.1.
AF279892 Genomic DNA. Translation: AAK69490.1.
BC081688 mRNA. Translation: AAH81688.1.
RefSeqNP_071784.1. NM_022389.2.
XP_006230954.1. XM_006230892.1.
XP_006230955.1. XM_006230893.1.
XP_006230956.1. XM_006230894.1.
XP_006230957.1. XM_006230895.1.
UniGeneRn.228.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-4580360.
STRING10116.ENSRNOP00000028194.

Chemistry

ChEMBLCHEMBL4965.

Proteomic databases

PaxDbQ9Z2Z8.
PRIDEQ9Z2Z8.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000028195; ENSRNOP00000028194; ENSRNOG00000020776.
GeneID64191.
KEGGrno:64191.
UCSCRGD:621769. rat.

Organism-specific databases

CTD1717.
RGD621769. Dhcr7.

Phylogenomic databases

eggNOGNOG72042.
GeneTreeENSGT00390000000417.
HOGENOMHOG000193296.
HOVERGENHBG007825.
InParanoidQ9Z2Z8.
KOK00213.
OMATFAMIKG.
OrthoDBEOG75B85C.
PhylomeDBQ9Z2Z8.
TreeFamTF101180.

Enzyme and pathway databases

BRENDA1.3.1.21. 5301.
UniPathwayUPA00063.

Gene expression databases

GenevestigatorQ9Z2Z8.

Family and domain databases

InterProIPR001171. Ergosterol_biosynth_ERG4_ERG24.
IPR018083. Sterol_reductase_CS.
[Graphical view]
PfamPF01222. ERG4_ERG24. 1 hit.
[Graphical view]
PROSITEPS01017. STEROL_REDUCT_1. 1 hit.
PS01018. STEROL_REDUCT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio612852.
PROQ9Z2Z8.

Entry information

Entry nameDHCR7_RAT
AccessionPrimary (citable) accession number: Q9Z2Z8
Entry history
Integrated into UniProtKB/Swiss-Prot: September 13, 2005
Last sequence update: May 1, 1999
Last modified: April 16, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways