Reviewed, UniProtKB/Swiss-Prot Q9Z2Z8 (DHCR7_RAT)

Names and origin

Protein names

Recommended name:
    7-dehydrocholesterol reductase
      Short name=7-DHC reductase
    EC=1.3.1.21
Alternative name(s):
    Sterol Delta(7)-reductase

Gene names

Name:

Dhcr7

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Production of cholesterol by reduction of C7-C8 double bond of 7-dehydrocholesterol (7-DHC).
Catalytic activity	Cholesterol + NADP+ = cholesta-5,7-dien-3-beta-ol + NADPH.
Pathway	Steroid biosynthesis; cholesterol biosynthesis.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the ERG4/ERG24 family.

Ontologies

Keywords
Biological process	Cholesterol biosynthesis Lipid synthesis Steroid biosynthesis Sterol biosynthesis
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	cholesterol biosynthetic process Ref.2 Ref.3 Inferred from direct assay. Source: RGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW regulation of cholesterol biosynthetic process Ref.2 Inferred from direct assay. Source: RGD
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	7-dehydrocholesterol reductase activity Ref.3 Traceable author statement. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 471	471	7-dehydrocholesterol reductase		PRO_0000207504
Regions
	Transmembrane	36 – 56	21	Potential
	Transmembrane	95 – 115	21	Potential
	Transmembrane	144 – 164	21	Potential
	Transmembrane	173 – 193	21	Potential
	Transmembrane	233 – 253	21	Potential
	Transmembrane	262 – 282	21	Potential
	Transmembrane	302 – 322	21	Potential
	Transmembrane	327 – 347	21	Potential
	Transmembrane	416 – 436	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9Z2Z8-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: EBF0CBC4F4222FDB	FASTA	471	54,155
	10 20 30 40 50 60 MASKSQHNAS KAKNHNVKAE SQGQWGRAWE VDWFSLVSVI FLLLFAPFIV YYFIMACDQY 70 80 90 100 110 120 SCSLTAPILD VATGRASLAD IWAKTPPVTA KAAQLYALWV SFQVLLYSWL PDFCHRFLPG 130 140 150 160 170 180 YVGGVQEGAI TPAGIVNKYE VNGLQAWLIT HFLWFVNAYL LSWFSPTIIF DNWIPLLWCA 190 200 210 220 230 240 NILGYAVSTF AMIKGYLFPT SAEDCKFTGN FFYNYMMGIE FNPRIGKWFD FKLFFNGRPG 250 260 270 280 290 300 IVAWTLINLS FAAKQQELYG HVTNSMILVN VLQAIYVLDF FWNETWYLKT IDICHDHFGW 310 320 330 340 350 360 YLGWGDCVWL PYLYTLQGLY LVYHPVQLST PNALGVLLLG LVGYYIFRMT NHQKDLFRRT 370 380 390 400 410 420 DGHCLIWGKK PKAIECSYTS ADGLKHRSKL LVSGFWGVAR HFNYTGDLMG SLAYCLACGG 430 440 450 460 470 GHLLPYFYII YMTILLTHRC LRDEHRCANK YGRDWERYVA AVPYRLLPGI F

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Transmembrane configuration of sterol delta 7-reductase as a potential sterol sensing protein." Nishino H., Ishibashi T. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Liver.
[2]	"Cholesterol biosynthesis from lanosterol. Molecular cloning, tissue distribution, expression, chromosomal localization, and regulation of rat 7-dehydrocholesterol reductase, a Smith-Lemli-Opitz syndrome-related protein." Bae S.-H., Lee J.N., Fitzky B.U., Seong J., Paik Y.-K. J. Biol. Chem. 274:14624-14631(1999) [PubMed: 10329655] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley.
[3]	"Structure and alternative splicing of the rat 7-dehydrocholesterol reductase gene." Lee J.-N., Bae S.-H., Paik Y.-K. Biochim. Biophys. Acta 1576:148-156(2002) [PubMed: 12031495] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Kidney.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB016800 mRNA. Translation: BAA34306.1. AF071500 mRNA. Translation: AAD31383.1. AF272393 mRNA. Translation: AAM45144.1. AF279892 Genomic DNA. Translation: AAK69490.1. BC081688 mRNA. Translation: AAH81688.1.
IPI	IPI00210431.
RefSeq	NP_071784.1.
UniGene	Rn.228
3D structure databases
ModBase	Search...
Proteomic databases
PRIDE	Q9Z2Z8.
Genome annotation databases
Ensembl	ENSRNOG00000020776. Rattus norvegicus. [Contig view]
GeneID	64191.
KEGG	rno:64191.
Organism-specific databases
RGD	621769. Dhcr7.
Phylogenomic databases
HOVERGEN	Q9Z2Z8.
OMA	Q9Z2Z8. WAKTPPV.
Enzyme and pathway databases
BRENDA	1.3.1.21. 248.
Gene expression databases
ArrayExpress	Q9Z2Z8.
GermOnline	ENSRNOG00000020776. Rattus norvegicus.
Family and domain databases
InterPro	IPR001171. Ergosterol_biosynth_ERG4_ERG24. IPR018083. Sterol_reductase_CS. [Graphical view]
Pfam	PF01222. ERG4_ERG24. 1 hit. [Graphical view]
PROSITE	PS01017. STEROL_REDUCT_1. 1 hit. PS01018. STEROL_REDUCT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	612852.

Entry information

Entry name

DHCR7_RAT

Accession

Primary (citable) accession number: Q9Z2Z8

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 13, 2005
Last sequence update:	May 1, 1999
Last modified:	June 16, 2009
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)