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Protein

Mitochondrial carnitine/acylcarnitine carrier protein

Gene

Slc25a20

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mediates the transport of acylcarnitines of different length across the mitochondrial inner membrane from the cytosol to the mitochondrial matrix for their oxidation by the mitochondrial fatty acid-oxidation pathway.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiR-MMU-200425. Import of palmitoyl-CoA into the mitochondrial matrix.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial carnitine/acylcarnitine carrier protein
Alternative name(s):
Carnitine/acylcarnitine translocase
Short name:
CAC
Short name:
mCAC
Solute carrier family 25 member 20
Gene namesi
Name:Slc25a20
Synonyms:Cac, Cact
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1928738. Slc25a20.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 1211CytoplasmicSequence analysisAdd
BLAST
Transmembranei13 – 3119Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini32 – 7342Mitochondrial matrixSequence analysisAdd
BLAST
Transmembranei74 – 9320Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini94 – 11219CytoplasmicSequence analysisAdd
BLAST
Transmembranei113 – 13119Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini132 – 17039Mitochondrial matrixSequence analysisAdd
BLAST
Transmembranei171 – 19020Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini191 – 21121CytoplasmicSequence analysisAdd
BLAST
Transmembranei212 – 23019Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini231 – 26737Mitochondrial matrixSequence analysisAdd
BLAST
Transmembranei268 – 28720Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini288 – 30114CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cytoplasm Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • mitochondrial inner membrane Source: MGI
  • mitochondrion Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 301300Mitochondrial carnitine/acylcarnitine carrier proteinPRO_0000090630Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei148 – 1481N6-acetyllysineCombined sources
Modified residuei157 – 1571N6-acetyllysineCombined sources
Modified residuei170 – 1701N6-acetyllysine; alternateCombined sources
Modified residuei170 – 1701N6-succinyllysine; alternateCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9Z2Z6.
MaxQBiQ9Z2Z6.
PaxDbiQ9Z2Z6.
PRIDEiQ9Z2Z6.

PTM databases

iPTMnetiQ9Z2Z6.
PhosphoSiteiQ9Z2Z6.
SwissPalmiQ9Z2Z6.

Expressioni

Tissue specificityi

Widely expressed, with highest levels in the liver, intermediate levels in heart, testis and kidney and low levels in brain, including cortex, cerebellum, hippocampus and hypothalamus.1 Publication

Gene expression databases

BgeeiQ9Z2Z6.
GenevisibleiQ9Z2Z6. MM.

Interactioni

Protein-protein interaction databases

IntActiQ9Z2Z6. 1 interaction.
STRINGi10090.ENSMUSP00000035222.

Structurei

3D structure databases

ProteinModelPortaliQ9Z2Z6.
SMRiQ9Z2Z6. Positions 13-289.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati8 – 9992Solcar 1Add
BLAST
Repeati108 – 19689Solcar 2Add
BLAST
Repeati207 – 29387Solcar 3Add
BLAST

Sequence similaritiesi

Contains 3 Solcar repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0758. Eukaryota.
ENOG410XS86. LUCA.
GeneTreeiENSGT00700000104295.
HOGENOMiHOG000168307.
HOVERGENiHBG003500.
InParanoidiQ9Z2Z6.
KOiK15109.
OMAiLSYPQIF.
OrthoDBiEOG7TTQ8C.
PhylomeDBiQ9Z2Z6.
TreeFamiTF300894.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z2Z6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADEPKPISP FKNLLAGGFG GMCLVFVGHP LDTVKVRLQT QPPSLSGQPP
60 70 80 90 100
MYSGTLDCFR KTLMREGITG LYRGMAAPII GVTPMFAVCF FGFGLGKKLQ
110 120 130 140 150
QKSPEDELSY PQLFTAGMLS GVFTTGIMTP GERIKCLLQI QASSGENKYS
160 170 180 190 200
GTLDCAKKLY QEFGIRGFYK GTVLTLMRDV PASGMYFMTY EWLKNLFTPE
210 220 230 240 250
GKSVSDLSVP RILVAGGFAG IFNWAVAIPP DVLKSRFQTA PPGKYPNGFR
260 270 280 290 300
DVLRELIREE GVTSLYKGFN AVMIRAFPAN AACFLGFEIA MKFLNWIAPN

L
Length:301
Mass (Da):33,027
Last modified:May 1, 1999 - v1
Checksum:i0E332BF4D7584490
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017112 mRNA. Translation: BAA74768.1.
AK075624 mRNA. Translation: BAC35865.1.
AK152436 mRNA. Translation: BAE31218.1.
BC029733 mRNA. Translation: AAH29733.1.
CCDSiCCDS23535.1.
RefSeqiNP_065266.1. NM_020520.4.
UniGeneiMm.29666.

Genome annotation databases

EnsembliENSMUST00000035222; ENSMUSP00000035222; ENSMUSG00000032602.
GeneIDi57279.
KEGGimmu:57279.
UCSCiuc009rqs.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB017112 mRNA. Translation: BAA74768.1.
AK075624 mRNA. Translation: BAC35865.1.
AK152436 mRNA. Translation: BAE31218.1.
BC029733 mRNA. Translation: AAH29733.1.
CCDSiCCDS23535.1.
RefSeqiNP_065266.1. NM_020520.4.
UniGeneiMm.29666.

3D structure databases

ProteinModelPortaliQ9Z2Z6.
SMRiQ9Z2Z6. Positions 13-289.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9Z2Z6. 1 interaction.
STRINGi10090.ENSMUSP00000035222.

PTM databases

iPTMnetiQ9Z2Z6.
PhosphoSiteiQ9Z2Z6.
SwissPalmiQ9Z2Z6.

Proteomic databases

EPDiQ9Z2Z6.
MaxQBiQ9Z2Z6.
PaxDbiQ9Z2Z6.
PRIDEiQ9Z2Z6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035222; ENSMUSP00000035222; ENSMUSG00000032602.
GeneIDi57279.
KEGGimmu:57279.
UCSCiuc009rqs.1. mouse.

Organism-specific databases

CTDi788.
MGIiMGI:1928738. Slc25a20.

Phylogenomic databases

eggNOGiKOG0758. Eukaryota.
ENOG410XS86. LUCA.
GeneTreeiENSGT00700000104295.
HOGENOMiHOG000168307.
HOVERGENiHBG003500.
InParanoidiQ9Z2Z6.
KOiK15109.
OMAiLSYPQIF.
OrthoDBiEOG7TTQ8C.
PhylomeDBiQ9Z2Z6.
TreeFamiTF300894.

Enzyme and pathway databases

ReactomeiR-MMU-200425. Import of palmitoyl-CoA into the mitochondrial matrix.

Miscellaneous databases

PROiQ9Z2Z6.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Z2Z6.
GenevisibleiQ9Z2Z6. MM.

Family and domain databases

Gene3Di1.50.40.10. 1 hit.
InterProiIPR018108. Mitochondrial_sb/sol_carrier.
IPR023395. Mt_carrier_dom.
[Graphical view]
PfamiPF00153. Mito_carr. 3 hits.
[Graphical view]
SUPFAMiSSF103506. SSF103506. 1 hit.
PROSITEiPS50920. SOLCAR. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, genomic structure and chromosomal localization of murine mitochondrial carnitine-acylcarnitine translocase gene and mutation analysis in the juvenile visceral steatosis (JVS) mouse."
    Lu K., Kuwajima M., Shima K., Nakamura Y., Nishimori H.
    Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Bone marrow.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II.
    Tissue: Mammary gland.
  4. "The human and mouse SLC25A29 mitochondrial transporters rescue the deficient ornithine metabolism in fibroblasts of patients with the hyperornithinemia-hyperammonemia-homocitrullinuria (HHH) syndrome."
    Camacho J.A., Rioseco-Camacho N.
    Pediatr. Res. 66:35-41(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Heart, Kidney, Liver, Lung, Spleen and Testis.
  6. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-170, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  7. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-148; LYS-157 AND LYS-170, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiMCAT_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2Z6
Secondary accession number(s): Q3U801
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 11, 2002
Last sequence update: May 1, 1999
Last modified: June 8, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.