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Protein

Homer protein homolog 1

Gene

Homer1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Differentially regulates the functions of the calcium activated channel ryanodine receptors RYR1 and RYR2. Isoform 1 decreases the activity of RYR2, and increases the activity of RYR1, whereas isoform 5 counteracts the effects by competing for binding sites. Isoform 3 regulates the trafficking and surface expression of GRM5. Isoform 5 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1, isoform 2 and isoform 3 to regulate synaptic metabotropic glutamate function. Isoform 5, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • behavioral response to cocaine Source: MGI
  • chemical homeostasis within a tissue Source: MGI
  • G-protein coupled glutamate receptor signaling pathway Source: MGI
  • positive regulation of calcium ion transport Source: MGI
  • regulation of calcium ion import Source: BHF-UCL
  • regulation of cation channel activity Source: BHF-UCL
  • regulation of store-operated calcium entry Source: BHF-UCL
  • response to calcium ion Source: MGI
  • skeletal muscle contraction Source: MGI
  • skeletal muscle fiber development Source: MGI
Complete GO annotation...

Protein family/group databases

TCDBi8.A.29.1.1. the homer1 (homer1) family of excitation-contraction coupling proteins.

Names & Taxonomyi

Protein namesi
Recommended name:
Homer protein homolog 1
Short name:
Homer-1
Alternative name(s):
VASP/Ena-related gene up-regulated during seizure and LTP 1
Short name:
Vesl-1
Gene namesi
Name:Homer1
Synonyms:Vesl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1347345. Homer1.

Subcellular locationi

GO - Cellular componenti

  • apical part of cell Source: MGI
  • axon Source: MGI
  • cell junction Source: UniProtKB-KW
  • costamere Source: MGI
  • cytoplasm Source: MGI
  • membrane Source: MGI
  • neuronal postsynaptic density Source: BHF-UCL
  • neuron projection Source: BHF-UCL
  • plasma membrane Source: MGI
  • postsynapse Source: MGI
  • postsynaptic density Source: CACAO
  • postsynaptic membrane Source: UniProtKB-KW
  • Z disc Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cytoplasm, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 366366Homer protein homolog 1PRO_0000191006Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei318 – 3181PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9Z2Y3.
PaxDbiQ9Z2Y3.
PRIDEiQ9Z2Y3.

PTM databases

iPTMnetiQ9Z2Y3.
PhosphoSiteiQ9Z2Y3.

Expressioni

Tissue specificityi

Isoform 1, isoform 3 and isoform 5 are expressed in skeletal muscle at the level of the Z line, in heart, forebrain and cerebellum. Isoform 2, is a minor isoform and is expressed in cardiac and skeletal muscle. Isoform 5 is expressed in the postsynaptic region of neurons.2 Publications

Gene expression databases

BgeeiQ9Z2Y3.
CleanExiMM_HOMER1.
ExpressionAtlasiQ9Z2Y3. baseline and differential.
GenevisibleiQ9Z2Y3. MM.

Interactioni

Subunit structurei

Isoform 1, isoform 2 and isoform 3 encode a coiled-coil structure that mediates homo- and heteromultimerization. Interacts with GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. Interacts with IFT57 and OPHN1.5 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi205014. 20 interactions.
IntActiQ9Z2Y3. 14 interactions.
MINTiMINT-3370083.
STRINGi10090.ENSMUSP00000078093.

Structurei

3D structure databases

ProteinModelPortaliQ9Z2Y3.
SMRiQ9Z2Y3. Positions 1-143, 302-364.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 110110WH1PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili193 – 364172Sequence analysisAdd
BLAST

Domaini

The WH1 domain interacts with the PPXXF motif in GRM1, GRM5, RYR1, RYR2, ITPR1, SHANK 1 and SHANK3.

Sequence similaritiesi

Belongs to the Homer family.Curated
Contains 1 WH1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IGRQ. Eukaryota.
ENOG410XQWT. LUCA.
GeneTreeiENSGT00390000017850.
HOGENOMiHOG000006979.
HOVERGENiHBG051918.
InParanoidiQ9Z2Y3.
KOiK15010.
OMAiQNSEPRP.
PhylomeDBiQ9Z2Y3.
TreeFamiTF325627.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR000697. WH1/EVH1_dom.
[Graphical view]
PfamiPF00568. WH1. 1 hit.
[Graphical view]
SMARTiSM00461. WH1. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS50229. WH1. 1 hit.
[Graphical view]

Sequences (5)i

Sequence statusi: Complete.

This entry describes 5 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Z2Y3-1) [UniParc]FASTAAdd to basket

Also known as: Vesl-1L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGEQPIFSTR AHVFQIDPNT KKNWVPTSKH AVTVSYFYDS TRNVYRIISL
60 70 80 90 100
DGSKAIINST ITPNMTFTKT SQKFGQWADS RANTVYGLGF SSEHHLSKFA
110 120 130 140 150
EKFQEFKEAA RLAKEKSQEK MELTSTPSQE SAGGDLQSPL TPESINGTDD
160 170 180 190 200
ERTPDVTQNS EPRAEPTQNA LPFPHSAGDR TQALSHASSA ISKHWEAELA
210 220 230 240 250
TLKGNNAKLT AALLESTANV KQWKQQLAAY QEEAERLHKR VTELECVSSQ
260 270 280 290 300
ANAVHSHKTE LNQTVQELEE TLKVKEEEIE RLKQEIDNAR ELQEQRDSLT
310 320 330 340 350
QKLQEVEIRN KDLEGQLSDL EQRLEKSQNE QEAFRSNLKT LLEILDGKIF
360
ELTELRDNLA KLLECS
Length:366
Mass (Da):41,413
Last modified:November 28, 2003 - v2
Checksum:i6A4CBCE5B8A91A3F
GO
Isoform 2 (identifier: Q9Z2Y3-2) [UniParc]FASTAAdd to basket

Also known as: 1d

The sequence of this isoform differs from the canonical sequence as follows:
     1-2: MG → MLIHHHNRRALCKGSPTT
     176-187: Missing.

Show »
Length:370
Mass (Da):42,084
Checksum:iABBDD4A4184E2233
GO
Isoform 3 (identifier: Q9Z2Y3-3) [UniParc]FASTAAdd to basket

Also known as: 1b

The sequence of this isoform differs from the canonical sequence as follows:
     176-187: Missing.

Show »
Length:354
Mass (Da):40,218
Checksum:iF01D84B0A87E168C
GO
Isoform 4 (identifier: Q9Z2Y3-4) [UniParc]FASTAAdd to basket

Also known as: Vesl-1M

The sequence of this isoform differs from the canonical sequence as follows:
     177-203: AGDRTQALSHASSAISKHWEAELATLK → SSRWIFFPYCEDSQLSLLESSSGLGYF
     204-366: Missing.

Show »
Length:203
Mass (Da):22,775
Checksum:i19461F47996D04C3
GO
Isoform 5 (identifier: Q9Z2Y3-5) [UniParc]FASTAAdd to basket

Also known as: Vesl-1S

The sequence of this isoform differs from the canonical sequence as follows:
     176-186: SAGDRTQALSH → RYTFNSAIMIK
     187-366: Missing.

Show »
Length:186
Mass (Da):20,912
Checksum:i9845DA75270BBF83
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti193 – 1931K → N in BAA34354 (Ref. 3) Curated
Sequence conflicti197 – 1971A → V in BAA34354 (Ref. 3) Curated
Sequence conflicti205 – 2051N → T in BAA34354 (Ref. 3) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 22MG → MLIHHHNRRALCKGSPTT in isoform 2. 1 PublicationVSP_009060
Alternative sequencei176 – 18712Missing in isoform 2 and isoform 3. 2 PublicationsVSP_009061Add
BLAST
Alternative sequencei176 – 18611SAGDRTQALSH → RYTFNSAIMIK in isoform 5. 2 PublicationsVSP_009062Add
BLAST
Alternative sequencei177 – 20327AGDRT…LATLK → SSRWIFFPYCEDSQLSLLES SSGLGYF in isoform 4. 1 PublicationVSP_009063Add
BLAST
Alternative sequencei187 – 366180Missing in isoform 5. 2 PublicationsVSP_009064Add
BLAST
Alternative sequencei204 – 366163Missing in isoform 4. 1 PublicationVSP_009065Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF093257 mRNA. Translation: AAC71021.1.
AF093258 mRNA. Translation: AAC71022.1.
AY137385 mRNA. Translation: AAM95461.1.
AB019478 mRNA. Translation: BAA34353.1.
AB019479 mRNA. Translation: BAA34354.1.
AB089435 mRNA. Translation: BAC07257.1.
CCDSiCCDS26687.1. [Q9Z2Y3-5]
CCDS36745.1. [Q9Z2Y3-1]
CCDS36746.1. [Q9Z2Y3-2]
CCDS70484.1. [Q9Z2Y3-3]
RefSeqiNP_001271118.1. NM_001284189.1. [Q9Z2Y3-3]
NP_036112.1. NM_011982.3. [Q9Z2Y3-5]
NP_671705.2. NM_147176.3. [Q9Z2Y3-1]
NP_687036.1. NM_152134.3. [Q9Z2Y3-2]
UniGeneiMm.37533.

Genome annotation databases

EnsembliENSMUST00000060490; ENSMUSP00000050471; ENSMUSG00000007617. [Q9Z2Y3-3]
ENSMUST00000079086; ENSMUSP00000078093; ENSMUSG00000007617. [Q9Z2Y3-2]
ENSMUST00000080127; ENSMUSP00000079026; ENSMUSG00000007617. [Q9Z2Y3-1]
ENSMUST00000102752; ENSMUSP00000099813; ENSMUSG00000007617. [Q9Z2Y3-5]
ENSMUST00000109492; ENSMUSP00000105118; ENSMUSG00000007617. [Q9Z2Y3-4]
GeneIDi26556.
KEGGimmu:26556.
UCSCiuc007rlc.3. mouse. [Q9Z2Y3-1]
uc007rld.3. mouse. [Q9Z2Y3-3]
uc007rle.3. mouse. [Q9Z2Y3-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF093257 mRNA. Translation: AAC71021.1.
AF093258 mRNA. Translation: AAC71022.1.
AY137385 mRNA. Translation: AAM95461.1.
AB019478 mRNA. Translation: BAA34353.1.
AB019479 mRNA. Translation: BAA34354.1.
AB089435 mRNA. Translation: BAC07257.1.
CCDSiCCDS26687.1. [Q9Z2Y3-5]
CCDS36745.1. [Q9Z2Y3-1]
CCDS36746.1. [Q9Z2Y3-2]
CCDS70484.1. [Q9Z2Y3-3]
RefSeqiNP_001271118.1. NM_001284189.1. [Q9Z2Y3-3]
NP_036112.1. NM_011982.3. [Q9Z2Y3-5]
NP_671705.2. NM_147176.3. [Q9Z2Y3-1]
NP_687036.1. NM_152134.3. [Q9Z2Y3-2]
UniGeneiMm.37533.

3D structure databases

ProteinModelPortaliQ9Z2Y3.
SMRiQ9Z2Y3. Positions 1-143, 302-364.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi205014. 20 interactions.
IntActiQ9Z2Y3. 14 interactions.
MINTiMINT-3370083.
STRINGi10090.ENSMUSP00000078093.

Protein family/group databases

TCDBi8.A.29.1.1. the homer1 (homer1) family of excitation-contraction coupling proteins.

PTM databases

iPTMnetiQ9Z2Y3.
PhosphoSiteiQ9Z2Y3.

Proteomic databases

MaxQBiQ9Z2Y3.
PaxDbiQ9Z2Y3.
PRIDEiQ9Z2Y3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000060490; ENSMUSP00000050471; ENSMUSG00000007617. [Q9Z2Y3-3]
ENSMUST00000079086; ENSMUSP00000078093; ENSMUSG00000007617. [Q9Z2Y3-2]
ENSMUST00000080127; ENSMUSP00000079026; ENSMUSG00000007617. [Q9Z2Y3-1]
ENSMUST00000102752; ENSMUSP00000099813; ENSMUSG00000007617. [Q9Z2Y3-5]
ENSMUST00000109492; ENSMUSP00000105118; ENSMUSG00000007617. [Q9Z2Y3-4]
GeneIDi26556.
KEGGimmu:26556.
UCSCiuc007rlc.3. mouse. [Q9Z2Y3-1]
uc007rld.3. mouse. [Q9Z2Y3-3]
uc007rle.3. mouse. [Q9Z2Y3-2]

Organism-specific databases

CTDi9456.
MGIiMGI:1347345. Homer1.

Phylogenomic databases

eggNOGiENOG410IGRQ. Eukaryota.
ENOG410XQWT. LUCA.
GeneTreeiENSGT00390000017850.
HOGENOMiHOG000006979.
HOVERGENiHBG051918.
InParanoidiQ9Z2Y3.
KOiK15010.
OMAiQNSEPRP.
PhylomeDBiQ9Z2Y3.
TreeFamiTF325627.

Miscellaneous databases

PROiQ9Z2Y3.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Z2Y3.
CleanExiMM_HOMER1.
ExpressionAtlasiQ9Z2Y3. baseline and differential.
GenevisibleiQ9Z2Y3. MM.

Family and domain databases

Gene3Di2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR000697. WH1/EVH1_dom.
[Graphical view]
PfamiPF00568. WH1. 1 hit.
[Graphical view]
SMARTiSM00461. WH1. 1 hit.
[Graphical view]
SUPFAMiSSF50729. SSF50729. 1 hit.
PROSITEiPS50229. WH1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Homer regulates the association of group 1 metabotropic glutamate receptors with multivalent complexes of homer-related, synaptic proteins."
    Xiao B., Tu J.C., Petralia R.S., Yuan J.P., Doan A., Breder C.D., Ruggiero A., Lanahan A.A., Wenthold R.J., Worley P.F.
    Neuron 21:707-716(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3 AND 5).
    Strain: C57BL/6J.
    Tissue: Forebrain.
  2. "An N-terminal sequence specific for a novel Homer1 isoform controls trafficking of group I metabotropic glutamate receptor in mammalian cells."
    Saito H., Kimura M., Inanobe A., Ohe T., Kurachi Y.
    Biochem. Biophys. Res. Commun. 296:523-529(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
    Strain: BALB/cJ.
    Tissue: Heart.
  3. "Mouse Vesl family of EVH-protein that interacts with group I mGluRs."
    Inokuchi K., Hayashi F.
    Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 5).
    Strain: C57BL/6N.
    Tissue: Brain cortex.
  4. "Genomic structure of mouse vesl-1 gene."
    Hayashi F., Hirai K., Inokuchi K.
    Submitted (AUG-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 4).
    Strain: 129/SvJ.
    Tissue: Hippocampus.
  5. "Homer binds a novel proline-rich motif and links group 1 metabotropic glutamate receptors with IP3 receptors."
    Tu J.C., Xiao B., Yuan J.P., Lanahan A.A., Leoffert K., Li M., Linden D.J., Worley P.F.
    Neuron 21:717-726(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GRM1; GRM5; DYN3 AND ITPR1.
  6. "Homer: a link between neural activity and glutamate receptor function."
    Xiao B., Tu J.C., Worley P.F.
    Curr. Opin. Neurobiol. 10:370-374(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW.
  7. "Homer proteins and InsP(3) receptors co-localise in the longitudinal sarcoplasmic reticulum of skeletal muscle fibres."
    Salanova M., Priori G., Barone V., Intravaia E., Flucher B., Ciruela F., McIlhinney R.A.J., Parys J.B., Mikoshiba K., Sorrentino V.
    Cell Calcium 32:193-200(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INTERACTION WITH ITPR1.
  8. "Homer1c interacts with Hippi and protects striatal neurons from apoptosis."
    Sakamoto K., Yoshida S., Ikegami K., Minakami R., Kato A., Udo H., Sugiyama H.
    Biochem. Biophys. Res. Commun. 352:1-5(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH IFT57.
  9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-318, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  10. Cited for: INTERACTION WITH SHANK3.
  11. "SHANK3 overexpression causes manic-like behaviour with unique pharmacogenetic properties."
    Han K., Holder J.L. Jr., Schaaf C.P., Lu H., Chen H., Kang H., Tang J., Wu Z., Hao S., Cheung S.W., Yu P., Sun H., Breman A.M., Patel A., Lu H.C., Zoghbi H.Y.
    Nature 503:72-77(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, INTERACTION WITH SHANK3, SUBCELLULAR LOCATION.

Entry informationi

Entry nameiHOME1_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2Y3
Secondary accession number(s): Q8K3E1
, Q8K4M8, Q9Z0E9, Q9Z216
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2003
Last sequence update: November 28, 2003
Last modified: June 8, 2016
This is version 138 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.