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Q9Z2Y2

- B4GT2_MOUSE

UniProt

Q9Z2Y2 - B4GT2_MOUSE

Protein

Beta-1,4-galactosyltransferase 2

Gene

B4galt2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. Can produce lactose By similarity.By similarity

    Catalytic activityi

    UDP-alpha-D-galactose + D-glucose = UDP + lactose.
    UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide.
    UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.

    Cofactori

    Manganese.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi215 – 2151ManganeseBy similarity
    Binding sitei275 – 2751UDP-alpha-D-galactoseBy similarity
    Metal bindingi308 – 3081Manganese; via tele nitrogenBy similarity
    Binding sitei320 – 3201N-acetyl-D-glucosamineBy similarity

    GO - Molecular functioni

    1. beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity Source: UniProtKB-EC
    2. lactose synthase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW
    4. N-acetyllactosamine synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_198565. N-Glycan antennae elongation.
    REACT_198578. Keratan sulfate biosynthesis.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT7. Glycosyltransferase Family 7.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-1,4-galactosyltransferase 2 (EC:2.4.1.-)
    Short name:
    Beta-1,4-GalTase 2
    Short name:
    Beta4Gal-T2
    Short name:
    b4Gal-T2
    Alternative name(s):
    UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 2
    UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 2
    Including the following 4 domains:
    Lactose synthase A protein (EC:2.4.1.22)
    N-acetyllactosamine synthase (EC:2.4.1.90)
    Alternative name(s):
    Nal synthase
    Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase (EC:2.4.1.38)
    Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase (EC:2.4.1.-)
    Gene namesi
    Name:B4galt2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 4

    Organism-specific databases

    MGIiMGI:1858493. B4galt2.

    Subcellular locationi

    Golgi apparatusGolgi stack membrane By similarity; Single-pass type II membrane protein By similarity
    Note: Trans cisternae of Golgi stack.By similarity

    GO - Cellular componenti

    1. Golgi cisterna membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 369369Beta-1,4-galactosyltransferase 2PRO_0000080534Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi63 – 631N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi68 – 681N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi94 ↔ 136By similarity
    Disulfide bondi208 ↔ 227By similarity
    Glycosylationi354 – 3541N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiQ9Z2Y2.

    PTM databases

    PhosphoSiteiQ9Z2Y2.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9Z2Y2.
    BgeeiQ9Z2Y2.
    GenevestigatoriQ9Z2Y2.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z2Y2.
    SMRiQ9Z2Y2. Positions 94-362.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1515CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini37 – 369333LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei16 – 3621Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni147 – 1515UDP-alpha-D-galactose bindingBy similarity
    Regioni186 – 1883UDP-alpha-D-galactose bindingBy similarity
    Regioni214 – 2152UDP-alpha-D-galactose bindingBy similarity
    Regioni277 – 2804N-acetyl-D-glucosamine bindingBy similarity
    Regioni308 – 3103UDP-alpha-D-galactose bindingBy similarity

    Sequence similaritiesi

    Belongs to the glycosyltransferase 7 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG327897.
    GeneTreeiENSGT00740000115031.
    HOGENOMiHOG000231027.
    HOVERGENiHBG058334.
    InParanoidiQ9Z2Y2.
    KOiK07967.
    OMAiTNCSRPN.
    OrthoDBiEOG7060R0.
    PhylomeDBiQ9Z2Y2.
    TreeFamiTF312834.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR003859. Galactosyl_T.
    IPR027791. Galactosyl_T_C.
    IPR027995. Galactosyl_T_N.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PANTHERiPTHR19300. PTHR19300. 1 hit.
    PfamiPF02709. Glyco_transf_7C. 1 hit.
    PF13733. Glyco_transf_7N. 1 hit.
    [Graphical view]
    PRINTSiPR02050. B14GALTRFASE.
    SUPFAMiSSF53448. SSF53448. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9Z2Y2-1 [UniParc]FASTAAdd to Basket

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    MSRLLGGTLE RVCKAVLLLC LLHFLVAVIL YFDVYAQHLA FFSRFSTRSP    50
    AHALYPAASS STNCSRPNAT AASSGLPEVP SARPGPTAPV IPPCPDVPPG 100
    LVGRVVIEFT SPMPLERVQR ENPGVLLGGR YSPPDCTPAQ TVAVIIPFRH 150
    REHHLRYWLH YLHPMLRRQR LRYGVYVINQ HGEETFNRAK LLNVGFLEAL 200
    KEDAAYDCFI FSDVDLVPMD DRNLYRCGDQ PRHFAIAMDK FGFRLPYASY 250
    FGGVSGLSKA QFLRINGFPN EYWGWGGEDD DIFNRISLTG MKISRPDVRI 300
    GRYRMIKHDR DKHNEPNPQR FNKIQNTKMS MKWDGIGSVR YRVLEVSRQP 350
    LFTNITVDIG QPMSWLTQG 369
    Length:369
    Mass (Da):41,909
    Last modified:May 1, 1999 - v1
    Checksum:iEF4465AFE51C6C30
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF142670 mRNA. Translation: AAF22220.1.
    AB019541 mRNA. Translation: BAA34385.1.
    AK165829 mRNA. Translation: BAE38399.1.
    CCDSiCCDS18539.1.
    RefSeqiNP_001240310.1. NM_001253381.1.
    NP_059073.1. NM_017377.5.
    XP_006503293.1. XM_006503230.1.
    UniGeneiMm.123843.

    Genome annotation databases

    EnsembliENSMUST00000030266; ENSMUSP00000030266; ENSMUSG00000028541.
    ENSMUST00000084325; ENSMUSP00000081352; ENSMUSG00000028541.
    ENSMUST00000106421; ENSMUSP00000102029; ENSMUSG00000028541.
    GeneIDi53418.
    KEGGimmu:53418.
    UCSCiuc008ujb.2. mouse.

    Cross-referencesi

    Web resourcesi

    Functional Glycomics Gateway - GTase

    b4GalT2

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF142670 mRNA. Translation: AAF22220.1 .
    AB019541 mRNA. Translation: BAA34385.1 .
    AK165829 mRNA. Translation: BAE38399.1 .
    CCDSi CCDS18539.1.
    RefSeqi NP_001240310.1. NM_001253381.1.
    NP_059073.1. NM_017377.5.
    XP_006503293.1. XM_006503230.1.
    UniGenei Mm.123843.

    3D structure databases

    ProteinModelPortali Q9Z2Y2.
    SMRi Q9Z2Y2. Positions 94-362.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GT7. Glycosyltransferase Family 7.

    PTM databases

    PhosphoSitei Q9Z2Y2.

    Proteomic databases

    PRIDEi Q9Z2Y2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000030266 ; ENSMUSP00000030266 ; ENSMUSG00000028541 .
    ENSMUST00000084325 ; ENSMUSP00000081352 ; ENSMUSG00000028541 .
    ENSMUST00000106421 ; ENSMUSP00000102029 ; ENSMUSG00000028541 .
    GeneIDi 53418.
    KEGGi mmu:53418.
    UCSCi uc008ujb.2. mouse.

    Organism-specific databases

    CTDi 8704.
    MGIi MGI:1858493. B4galt2.

    Phylogenomic databases

    eggNOGi NOG327897.
    GeneTreei ENSGT00740000115031.
    HOGENOMi HOG000231027.
    HOVERGENi HBG058334.
    InParanoidi Q9Z2Y2.
    KOi K07967.
    OMAi TNCSRPN.
    OrthoDBi EOG7060R0.
    PhylomeDBi Q9Z2Y2.
    TreeFami TF312834.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .
    Reactomei REACT_198565. N-Glycan antennae elongation.
    REACT_198578. Keratan sulfate biosynthesis.

    Miscellaneous databases

    NextBioi 310245.
    PROi Q9Z2Y2.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9Z2Y2.
    Bgeei Q9Z2Y2.
    Genevestigatori Q9Z2Y2.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR003859. Galactosyl_T.
    IPR027791. Galactosyl_T_C.
    IPR027995. Galactosyl_T_N.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    PANTHERi PTHR19300. PTHR19300. 1 hit.
    Pfami PF02709. Glyco_transf_7C. 1 hit.
    PF13733. Glyco_transf_7N. 1 hit.
    [Graphical view ]
    PRINTSi PR02050. B14GALTRFASE.
    SUPFAMi SSF53448. SSF53448. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The expanding beta 4-galactosyltransferase gene family: messages from the databanks."
      Lo N.-W., Shaper J.H., Pevsner J., Shaper N.L.
      Glycobiology 8:517-526(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Mouse beta-1,4-galactosyltransferase II (beta-1,4-GalT II)."
      Sato T.
      Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    3. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Lung.

    Entry informationi

    Entry nameiB4GT2_MOUSE
    AccessioniPrimary (citable) accession number: Q9Z2Y2
    Secondary accession number(s): Q3TMP2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 21, 2005
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 105 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3