Reviewed, UniProtKB/Swiss-Prot Q9Z2Y2 (B4GT2_MOUSE)

Names and origin

Protein names

Recommended name:
    Beta-1,4-galactosyltransferase 2
      Short name=Beta-1,4-GalTase 2
      Short name=Beta4Gal-T2
      Short name=b4Gal-T2
    EC=2.4.1.-
Alternative name(s):
    UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 2
    UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 2
Including the following 4 domains:
    1- Recommended name:
            Lactose synthase A protein
              EC=2.4.1.22
    2- Recommended name:
            N-acetyllactosamine synthase
              EC=2.4.1.90
        Alternative name(s):
            Nal synthetase
    3- Recommended name:
            Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase
              EC=2.4.1.38
    4- Recommended name:
            Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase
              EC=2.4.1.-

Gene names

Name:

B4galt2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	369 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. Can produce lactose By similarity.
Catalytic activity	UDP-galactose + D-glucose = UDP + lactose. UDP-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide. UDP-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.
Cofactor	Manganese By similarity.
Pathway	Protein modification; protein glycosylation.
Subcellular location	Golgi apparatus › Golgi stack membrane; Single-pass type II membrane protein By similarity. Note: Trans cisternae of Golgi stack By similarity.
Sequence similarities	Belongs to the glycosyltransferase 7 family.

Ontologies

Keywords
Cellular component	Golgi apparatus Membrane
Domain	Signal-anchor Transmembrane
Ligand	Manganese Metal-binding
Molecular function	Glycosyltransferase Transferase
PTM	Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetyllactosamine synthase activity Inferred from electronic annotation. Source: EC beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity Inferred from electronic annotation. Source: EC lactose synthase activity Inferred from electronic annotation. Source: EC manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 369	369	Beta-1,4-galactosyltransferase 2		PRO_0000080534
Regions
	Topological domain	1 – 15	15	Cytoplasmic Potential
	Transmembrane	16 – 36	21	Signal-anchor for type II membrane protein Potential
	Topological domain	37 – 369	333	Lumenal Potential
Sites
	Metal binding	215	1	Manganese By similarity
	Metal binding	308	1	Manganese By similarity
Amino acid modifications
	Glycosylation	63	1	N-linked (GlcNAc...) Potential
	Glycosylation	68	1	N-linked (GlcNAc...) Potential
	Glycosylation	354	1	N-linked (GlcNAc...) Potential
	Disulfide bond	94 ↔ 136		By similarity
	Disulfide bond	208 ↔ 227		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Z2Y2-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: EF4465AFE51C6C30	FASTA	369	41,909
	10 20 30 40 50 60 MSRLLGGTLE RVCKAVLLLC LLHFLVAVIL YFDVYAQHLA FFSRFSTRSP AHALYPAASS 70 80 90 100 110 120 STNCSRPNAT AASSGLPEVP SARPGPTAPV IPPCPDVPPG LVGRVVIEFT SPMPLERVQR 130 140 150 160 170 180 ENPGVLLGGR YSPPDCTPAQ TVAVIIPFRH REHHLRYWLH YLHPMLRRQR LRYGVYVINQ 190 200 210 220 230 240 HGEETFNRAK LLNVGFLEAL KEDAAYDCFI FSDVDLVPMD DRNLYRCGDQ PRHFAIAMDK 250 260 270 280 290 300 FGFRLPYASY FGGVSGLSKA QFLRINGFPN EYWGWGGEDD DIFNRISLTG MKISRPDVRI 310 320 330 340 350 360 GRYRMIKHDR DKHNEPNPQR FNKIQNTKMS MKWDGIGSVR YRVLEVSRQP LFTNITVDIG QPMSWLTQG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The expanding beta 4-galactosyltransferase gene family: messages from the databanks." Lo N.-W., Shaper J.H., Pevsner J., Shaper N.L. Glycobiology 8:517-526(1998) [PubMed: 9597550] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Mouse beta-1,4-galactosyltransferase II (beta-1,4-GalT II)." Sato T. Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[3]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
+	Additional computationally mapped references.

Web resources

Functional Glycomics Gateway - GTase

b4GalT2

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF142670 mRNA. Translation: AAF22220.1. AB019541 mRNA. Translation: BAA34385.1. AK165829 mRNA. Translation: BAE38399.1.
IPI	IPI00131542.
RefSeq	NP_059073.1.
UniGene	Mm.123843
3D structure databases
SMR	Q9Z2Y2. Positions 94-362.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9Z2Y2.
Protein family/group databases
CAZy	GT7. Glycosyltransferase Family 7.
Proteomic databases
PRIDE	Q9Z2Y2.
Genome annotation databases
Ensembl	ENSMUST00000030266; ENSMUSP00000030266; ENSMUSG00000028541; Mus musculus. [Genome view] ENSMUST00000106421; ENSMUSP00000102029; ENSMUSG00000028541; Mus musculus. [Genome view]
GeneID	53418.
KEGG	mmu:53418.
UCSC	uc008ujb.1. mouse.
Organism-specific databases
CTD	53418.
MGI	MGI:1858493. B4galt2.
Phylogenomic databases
eggNOG	roNOG04177.
HOGENOM	HBG717407.
HOVERGEN	Q9Z2Y2.
InParanoid	Q9Z2Y2.
OMA	YVINQHG.
OrthoDB	EOG9HTCCH.
PhylomeDB	Q9Z2Y2.
Enzyme and pathway databases
BRENDA	2.4.1.22. 244. 2.4.1.38. 244. 2.4.1.90. 244.
Gene expression databases
ArrayExpress	Q9Z2Y2.
Bgee	Q9Z2Y2.
Genevestigator	Q9Z2Y2.
GermOnline	ENSMUSG00000028541. Mus musculus.
Family and domain databases
InterPro	IPR003859. Galactosyl_T_2. [Graphical view]
PANTHER	PTHR19300. Galactosyl_T_2. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	310245.
SOURCE	Search...

Entry information

Entry name

B4GT2_MOUSE

Accession

Primary (citable) accession number: Q9Z2Y2
Secondary accession number(s): Q3TMP2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	May 1, 1999
Last modified:	February 9, 2010
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)