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Q9Z2Y2

- B4GT2_MOUSE

UniProt

Q9Z2Y2 - B4GT2_MOUSE

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Protein
Beta-1,4-galactosyltransferase 2
Gene
B4galt2
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. Can produce lactose By similarity.

Catalytic activityi

UDP-alpha-D-galactose + D-glucose = UDP + lactose.
UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminylglycopeptide = UDP + beta-D-galactosyl-(1->4)-N-acetyl-beta-D-glucosaminylglycopeptide.
UDP-alpha-D-galactose + N-acetyl-D-glucosamine = UDP + N-acetyllactosamine.

Cofactori

Manganese By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi215 – 2151Manganese By similarity
Binding sitei275 – 2751UDP-alpha-D-galactose By similarity
Metal bindingi308 – 3081Manganese; via tele nitrogen By similarity
Binding sitei320 – 3201N-acetyl-D-glucosamine By similarity

GO - Molecular functioni

  1. N-acetyllactosamine synthase activity Source: UniProtKB-EC
  2. beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase activity Source: UniProtKB-EC
  3. lactose synthase activity Source: UniProtKB-EC
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Manganese, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_198565. N-Glycan antennae elongation.
REACT_198578. Keratan sulfate biosynthesis.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT7. Glycosyltransferase Family 7.

Names & Taxonomyi

Protein namesi
Recommended name:
Beta-1,4-galactosyltransferase 2 (EC:2.4.1.-)
Short name:
Beta-1,4-GalTase 2
Short name:
Beta4Gal-T2
Short name:
b4Gal-T2
Alternative name(s):
UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 2
UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 2
Including the following 4 domains:
Lactose synthase A protein (EC:2.4.1.22)
N-acetyllactosamine synthase (EC:2.4.1.90)
Alternative name(s):
Nal synthase
Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase (EC:2.4.1.38)
Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase (EC:2.4.1.-)
Gene namesi
Name:B4galt2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 4

Organism-specific databases

MGIiMGI:1858493. B4galt2.

Subcellular locationi

Golgi apparatusGolgi stack membrane; Single-pass type II membrane protein By similarity
Note: Trans cisternae of Golgi stack By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1515Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei16 – 3621Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini37 – 369333Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi cisterna membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 369369Beta-1,4-galactosyltransferase 2
PRO_0000080534Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi63 – 631N-linked (GlcNAc...) Reviewed prediction
Glycosylationi68 – 681N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi94 ↔ 136 By similarity
Disulfide bondi208 ↔ 227 By similarity
Glycosylationi354 – 3541N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiQ9Z2Y2.

PTM databases

PhosphoSiteiQ9Z2Y2.

Expressioni

Gene expression databases

ArrayExpressiQ9Z2Y2.
BgeeiQ9Z2Y2.
GenevestigatoriQ9Z2Y2.

Structurei

3D structure databases

ProteinModelPortaliQ9Z2Y2.
SMRiQ9Z2Y2. Positions 94-362.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni147 – 1515UDP-alpha-D-galactose binding By similarity
Regioni186 – 1883UDP-alpha-D-galactose binding By similarity
Regioni214 – 2152UDP-alpha-D-galactose binding By similarity
Regioni277 – 2804N-acetyl-D-glucosamine binding By similarity
Regioni308 – 3103UDP-alpha-D-galactose binding By similarity

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG327897.
GeneTreeiENSGT00740000115031.
HOGENOMiHOG000231027.
HOVERGENiHBG058334.
InParanoidiQ9Z2Y2.
KOiK07967.
OMAiTNCSRPN.
OrthoDBiEOG7060R0.
PhylomeDBiQ9Z2Y2.
TreeFamiTF312834.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view]
PANTHERiPTHR19300. PTHR19300. 1 hit.
PfamiPF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view]
PRINTSiPR02050. B14GALTRFASE.
SUPFAMiSSF53448. SSF53448. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9Z2Y2-1 [UniParc]FASTAAdd to Basket

« Hide

MSRLLGGTLE RVCKAVLLLC LLHFLVAVIL YFDVYAQHLA FFSRFSTRSP    50
AHALYPAASS STNCSRPNAT AASSGLPEVP SARPGPTAPV IPPCPDVPPG 100
LVGRVVIEFT SPMPLERVQR ENPGVLLGGR YSPPDCTPAQ TVAVIIPFRH 150
REHHLRYWLH YLHPMLRRQR LRYGVYVINQ HGEETFNRAK LLNVGFLEAL 200
KEDAAYDCFI FSDVDLVPMD DRNLYRCGDQ PRHFAIAMDK FGFRLPYASY 250
FGGVSGLSKA QFLRINGFPN EYWGWGGEDD DIFNRISLTG MKISRPDVRI 300
GRYRMIKHDR DKHNEPNPQR FNKIQNTKMS MKWDGIGSVR YRVLEVSRQP 350
LFTNITVDIG QPMSWLTQG 369
Length:369
Mass (Da):41,909
Last modified:May 1, 1999 - v1
Checksum:iEF4465AFE51C6C30
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF142670 mRNA. Translation: AAF22220.1.
AB019541 mRNA. Translation: BAA34385.1.
AK165829 mRNA. Translation: BAE38399.1.
CCDSiCCDS18539.1.
RefSeqiNP_001240310.1. NM_001253381.1.
NP_059073.1. NM_017377.5.
XP_006503293.1. XM_006503230.1.
UniGeneiMm.123843.

Genome annotation databases

EnsembliENSMUST00000030266; ENSMUSP00000030266; ENSMUSG00000028541.
ENSMUST00000084325; ENSMUSP00000081352; ENSMUSG00000028541.
ENSMUST00000106421; ENSMUSP00000102029; ENSMUSG00000028541.
GeneIDi53418.
KEGGimmu:53418.
UCSCiuc008ujb.2. mouse.

Cross-referencesi

Web resourcesi

Functional Glycomics Gateway - GTase

b4GalT2

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF142670 mRNA. Translation: AAF22220.1 .
AB019541 mRNA. Translation: BAA34385.1 .
AK165829 mRNA. Translation: BAE38399.1 .
CCDSi CCDS18539.1.
RefSeqi NP_001240310.1. NM_001253381.1.
NP_059073.1. NM_017377.5.
XP_006503293.1. XM_006503230.1.
UniGenei Mm.123843.

3D structure databases

ProteinModelPortali Q9Z2Y2.
SMRi Q9Z2Y2. Positions 94-362.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GT7. Glycosyltransferase Family 7.

PTM databases

PhosphoSitei Q9Z2Y2.

Proteomic databases

PRIDEi Q9Z2Y2.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000030266 ; ENSMUSP00000030266 ; ENSMUSG00000028541 .
ENSMUST00000084325 ; ENSMUSP00000081352 ; ENSMUSG00000028541 .
ENSMUST00000106421 ; ENSMUSP00000102029 ; ENSMUSG00000028541 .
GeneIDi 53418.
KEGGi mmu:53418.
UCSCi uc008ujb.2. mouse.

Organism-specific databases

CTDi 8704.
MGIi MGI:1858493. B4galt2.

Phylogenomic databases

eggNOGi NOG327897.
GeneTreei ENSGT00740000115031.
HOGENOMi HOG000231027.
HOVERGENi HBG058334.
InParanoidi Q9Z2Y2.
KOi K07967.
OMAi TNCSRPN.
OrthoDBi EOG7060R0.
PhylomeDBi Q9Z2Y2.
TreeFami TF312834.

Enzyme and pathway databases

UniPathwayi UPA00378 .
Reactomei REACT_198565. N-Glycan antennae elongation.
REACT_198578. Keratan sulfate biosynthesis.

Miscellaneous databases

NextBioi 310245.
PROi Q9Z2Y2.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9Z2Y2.
Bgeei Q9Z2Y2.
Genevestigatori Q9Z2Y2.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR003859. Galactosyl_T.
IPR027791. Galactosyl_T_C.
IPR027995. Galactosyl_T_N.
IPR029044. Nucleotide-diphossugar_trans.
[Graphical view ]
PANTHERi PTHR19300. PTHR19300. 1 hit.
Pfami PF02709. Glyco_transf_7C. 1 hit.
PF13733. Glyco_transf_7N. 1 hit.
[Graphical view ]
PRINTSi PR02050. B14GALTRFASE.
SUPFAMi SSF53448. SSF53448. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The expanding beta 4-galactosyltransferase gene family: messages from the databanks."
    Lo N.-W., Shaper J.H., Pevsner J., Shaper N.L.
    Glycobiology 8:517-526(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Mouse beta-1,4-galactosyltransferase II (beta-1,4-GalT II)."
    Sato T.
    Submitted (NOV-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.

Entry informationi

Entry nameiB4GT2_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2Y2
Secondary accession number(s): Q3TMP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: May 1, 1999
Last modified: September 3, 2014
This is version 104 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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