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Protein

Heterogeneous nuclear ribonucleoprotein F

Gene

Hnrnpf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-6803529. FGFR2 alternative splicing.
R-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72203. Processing of Capped Intron-Containing Pre-mRNA.

Names & Taxonomyi

Protein namesi
Recommended name:
Heterogeneous nuclear ribonucleoprotein F
Short name:
hnRNP F
Cleaved into the following chain:
Gene namesi
Name:Hnrnpf
Synonyms:Hnrpf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2138741. Hnrnpf.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003671161 – 415Heterogeneous nuclear ribonucleoprotein FAdd BLAST415
Initiator methionineiRemoved; alternateBy similarity
ChainiPRO_00002530532 – 415Heterogeneous nuclear ribonucleoprotein F, N-terminally processedAdd BLAST414

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei2N-acetylmethionine; in Heterogeneous nuclear ribonucleoprotein F, N-terminally processedBy similarity1
Cross-linki72Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei104PhosphoserineBy similarity1
Modified residuei107PhosphoserineCombined sources1
Modified residuei161PhosphoserineBy similarity1
Modified residuei187PhosphoserineCombined sources1
Modified residuei193PhosphoserineBy similarity1
Modified residuei195PhosphoserineBy similarity1
Modified residuei200N6-acetyllysine; alternateCombined sources1
Cross-linki200Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei215PhosphothreonineBy similarity1
Modified residuei224N6-acetyllysineBy similarity1
Modified residuei265PhosphoserineBy similarity1

Post-translational modificationi

Sumoylated.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9Z2X1.
PaxDbiQ9Z2X1.
PeptideAtlasiQ9Z2X1.
PRIDEiQ9Z2X1.

2D gel databases

REPRODUCTION-2DPAGEQ9Z2X1.

PTM databases

iPTMnetiQ9Z2X1.
PhosphoSitePlusiQ9Z2X1.
SwissPalmiQ9Z2X1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000042079.
ExpressionAtlasiQ9Z2X1. baseline and differential.
GenevisibleiQ9Z2X1. MM.

Interactioni

Subunit structurei

Identified in the spliceosome C complex. Interacts with AGO1, AGO2, TBP and TXNL4/DIM1 (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei16Interaction with RNABy similarity1
Sitei20Interaction with RNABy similarity1
Sitei52Interaction with RNABy similarity1
Sitei75Interaction with RNABy similarity1
Sitei116Interaction with RNABy similarity1
Sitei120Interaction with RNABy similarity1
Sitei150Interaction with RNABy similarity1
Sitei173Interaction with RNABy similarity1
Sitei294Interaction with RNABy similarity1
Sitei298Interaction with RNABy similarity1
Sitei326Interaction with RNABy similarity1
Sitei349Interaction with RNABy similarity1

Protein-protein interaction databases

BioGridi221126. 37 interactors.
IntActiQ9Z2X1. 33 interactors.
MINTiMINT-1864385.
STRINGi10090.ENSMUSP00000045048.

Structurei

Secondary structure

1415
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 5Combined sources3
Beta strandi12 – 17Combined sources6
Helixi24 – 30Combined sources7
Turni31 – 33Combined sources3
Helixi39 – 42Combined sources4
Beta strandi43 – 47Combined sources5
Beta strandi49 – 51Combined sources3
Beta strandi53 – 63Combined sources11
Helixi64 – 70Combined sources7
Helixi71 – 73Combined sources3
Beta strandi76 – 78Combined sources3
Beta strandi81 – 88Combined sources8
Helixi90 – 98Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DB1NMR-A1-105[»]
ProteinModelPortaliQ9Z2X1.
SMRiQ9Z2X1.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Z2X1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 90RRM 1PROSITE-ProRule annotationAdd BLAST80
Domaini111 – 188RRM 2PROSITE-ProRule annotationAdd BLAST78
Domaini289 – 366RRM 3PROSITE-ProRule annotationAdd BLAST78

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni81 – 86Interaction with RNABy similarity6
Regioni179 – 184Interaction with RNABy similarity6
Regioni355 – 360Interaction with RNABy similarity6

Domaini

The N-terminal RRM domains are responsible for recognizing the G-tract of BCL-X RNA.By similarity

Sequence similaritiesi

Contains 3 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG4211. Eukaryota.
ENOG410Z6M0. LUCA.
GeneTreeiENSGT00760000119102.
HOGENOMiHOG000220896.
HOVERGENiHBG055557.
InParanoidiQ9Z2X1.
KOiK12898.
OMAiFLSECKI.
OrthoDBiEOG091G0CTJ.
PhylomeDBiQ9Z2X1.
TreeFamiTF316157.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR012996. Znf_CHHC.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
PF08080. zf-RNPHF. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 3 hits.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Z2X1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMLGPEGGEG YVVKLRGLPW SCSIEDVQNF LSDCTIHDGV AGVHFIYTRE
60 70 80 90 100
GRQSGEAFVE LESEDDVKLA LKKDRESMGH RYIEVFKSHR TEMDWVLKHS
110 120 130 140 150
GPNSADSAND GFVRLRGLPF GCTKEEIVQF FSGLEIVPNG ITLPVDPEGK
160 170 180 190 200
ITGEAFVQFA SQELAEKALG KHKERIGHRY IEVFKSSQEE VRSYSDPPLK
210 220 230 240 250
FMSVQRPGPY DRPGTARRYI GIVKQAGLDR MRSGAYSAGY GGYEEYSGLS
260 270 280 290 300
DGYGFTTDLF GRDLSYCLSG MYDHRYGDSE FTVQSTTGHC VHMRGLPYKA
310 320 330 340 350
TENDIYNFFS PLNPVRVHIE IGPDGRVTGE ADVEFATHEE AVAAMSKDRA
360 370 380 390 400
NMQHRYIELF LNSTTGASNG AYSSQVMQGM GVSAAQATYS GLESQSVSGC
410
YGAGYSGQNS MGGYD
Length:415
Mass (Da):45,730
Last modified:January 23, 2007 - v3
Checksum:i31E1C9C90A8E051F
GO
Isoform 2 (identifier: Q9Z2X1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: MMLGPEGGEGYVVKLRGLPWSC → MW

Show »
Length:395
Mass (Da):43,685
Checksum:i2EC531A33BA024CF
GO

Sequence cautioni

The sequence AAH27003 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH29764 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC36361 differs from that shown. Reason: Erroneous termination at position 416. Translated as stop.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti272Y → C in AAH27003 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0210041 – 22MMLGP…LPWSC → MW in isoform 2. 1 PublicationAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK076478 mRNA. Translation: BAC36361.1. Sequence problems.
BC018185 mRNA. Translation: AAH18185.1.
BC025481 mRNA. Translation: AAH25481.1.
BC027003 mRNA. Translation: AAH27003.1. Different initiation.
BC029163 mRNA. Translation: AAH29163.1.
BC029764 mRNA. Translation: AAH29764.1. Different initiation.
BC033483 mRNA. Translation: AAH33483.1.
BC089313 mRNA. Translation: AAH89313.1.
CCDSiCCDS20467.1. [Q9Z2X1-1]
RefSeqiNP_001159899.1. NM_001166427.1. [Q9Z2X1-1]
NP_001159900.1. NM_001166428.1. [Q9Z2X1-1]
NP_001159901.1. NM_001166429.1. [Q9Z2X1-1]
NP_001159902.1. NM_001166430.1. [Q9Z2X1-1]
NP_001159903.1. NM_001166431.1. [Q9Z2X1-1]
NP_001159904.1. NM_001166432.1. [Q9Z2X1-1]
NP_598595.1. NM_133834.2. [Q9Z2X1-1]
XP_006506892.1. XM_006506829.3. [Q9Z2X1-1]
UniGeneiMm.422979.
Mm.476420.

Genome annotation databases

EnsembliENSMUST00000035493; ENSMUSP00000045048; ENSMUSG00000042079. [Q9Z2X1-1]
ENSMUST00000163168; ENSMUSP00000130023; ENSMUSG00000042079. [Q9Z2X1-1]
ENSMUST00000167182; ENSMUSP00000126817; ENSMUSG00000042079. [Q9Z2X1-1]
ENSMUST00000180020; ENSMUSP00000137632; ENSMUSG00000042079. [Q9Z2X1-1]
ENSMUST00000180341; ENSMUSP00000136700; ENSMUSG00000042079. [Q9Z2X1-1]
GeneIDi98758.
KEGGimmu:98758.
UCSCiuc009dle.2. mouse. [Q9Z2X1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK076478 mRNA. Translation: BAC36361.1. Sequence problems.
BC018185 mRNA. Translation: AAH18185.1.
BC025481 mRNA. Translation: AAH25481.1.
BC027003 mRNA. Translation: AAH27003.1. Different initiation.
BC029163 mRNA. Translation: AAH29163.1.
BC029764 mRNA. Translation: AAH29764.1. Different initiation.
BC033483 mRNA. Translation: AAH33483.1.
BC089313 mRNA. Translation: AAH89313.1.
CCDSiCCDS20467.1. [Q9Z2X1-1]
RefSeqiNP_001159899.1. NM_001166427.1. [Q9Z2X1-1]
NP_001159900.1. NM_001166428.1. [Q9Z2X1-1]
NP_001159901.1. NM_001166429.1. [Q9Z2X1-1]
NP_001159902.1. NM_001166430.1. [Q9Z2X1-1]
NP_001159903.1. NM_001166431.1. [Q9Z2X1-1]
NP_001159904.1. NM_001166432.1. [Q9Z2X1-1]
NP_598595.1. NM_133834.2. [Q9Z2X1-1]
XP_006506892.1. XM_006506829.3. [Q9Z2X1-1]
UniGeneiMm.422979.
Mm.476420.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DB1NMR-A1-105[»]
ProteinModelPortaliQ9Z2X1.
SMRiQ9Z2X1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi221126. 37 interactors.
IntActiQ9Z2X1. 33 interactors.
MINTiMINT-1864385.
STRINGi10090.ENSMUSP00000045048.

PTM databases

iPTMnetiQ9Z2X1.
PhosphoSitePlusiQ9Z2X1.
SwissPalmiQ9Z2X1.

2D gel databases

REPRODUCTION-2DPAGEQ9Z2X1.

Proteomic databases

EPDiQ9Z2X1.
PaxDbiQ9Z2X1.
PeptideAtlasiQ9Z2X1.
PRIDEiQ9Z2X1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035493; ENSMUSP00000045048; ENSMUSG00000042079. [Q9Z2X1-1]
ENSMUST00000163168; ENSMUSP00000130023; ENSMUSG00000042079. [Q9Z2X1-1]
ENSMUST00000167182; ENSMUSP00000126817; ENSMUSG00000042079. [Q9Z2X1-1]
ENSMUST00000180020; ENSMUSP00000137632; ENSMUSG00000042079. [Q9Z2X1-1]
ENSMUST00000180341; ENSMUSP00000136700; ENSMUSG00000042079. [Q9Z2X1-1]
GeneIDi98758.
KEGGimmu:98758.
UCSCiuc009dle.2. mouse. [Q9Z2X1-1]

Organism-specific databases

CTDi3185.
MGIiMGI:2138741. Hnrnpf.

Phylogenomic databases

eggNOGiKOG4211. Eukaryota.
ENOG410Z6M0. LUCA.
GeneTreeiENSGT00760000119102.
HOGENOMiHOG000220896.
HOVERGENiHBG055557.
InParanoidiQ9Z2X1.
KOiK12898.
OMAiFLSECKI.
OrthoDBiEOG091G0CTJ.
PhylomeDBiQ9Z2X1.
TreeFamiTF316157.

Enzyme and pathway databases

ReactomeiR-MMU-6803529. FGFR2 alternative splicing.
R-MMU-72163. mRNA Splicing - Major Pathway.
R-MMU-72203. Processing of Capped Intron-Containing Pre-mRNA.

Miscellaneous databases

ChiTaRSiHnrnpf. mouse.
EvolutionaryTraceiQ9Z2X1.
PROiQ9Z2X1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042079.
ExpressionAtlasiQ9Z2X1. baseline and differential.
GenevisibleiQ9Z2X1. MM.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR012996. Znf_CHHC.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
PF08080. zf-RNPHF. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 3 hits.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHNRPF_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2X1
Secondary accession number(s): Q5FWK2
, Q8BVU8, Q8K2U9, Q8R0E7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 155 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.