Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Heterogeneous nuclear ribonucleoprotein F

Gene

Hnrnpf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Plays a role in the regulation of alternative splicing events. Binds G-rich sequences in pre-mRNAs and keeps target RNA in an unfolded state (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-6803529. FGFR2 alternative splicing.
R-MMU-72163. mRNA Splicing - Major Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Heterogeneous nuclear ribonucleoprotein F
Short name:
hnRNP F
Cleaved into the following chain:
Gene namesi
Name:Hnrnpf
Synonyms:Hnrpf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:2138741. Hnrnpf.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 415415Heterogeneous nuclear ribonucleoprotein FPRO_0000367116Add
BLAST
Initiator methionineiRemoved; alternateBy similarity
Chaini2 – 415414Heterogeneous nuclear ribonucleoprotein F, N-terminally processedPRO_0000253053Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei2 – 21N-acetylmethionine; in Heterogeneous nuclear ribonucleoprotein F, N-terminally processedBy similarity
Cross-linki72 – 72Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)By similarity
Modified residuei104 – 1041PhosphoserineBy similarity
Modified residuei107 – 1071PhosphoserineCombined sources
Modified residuei161 – 1611PhosphoserineBy similarity
Modified residuei187 – 1871PhosphoserineCombined sources
Modified residuei193 – 1931PhosphoserineBy similarity
Modified residuei195 – 1951PhosphoserineBy similarity
Modified residuei200 – 2001N6-acetyllysine; alternateCombined sources
Cross-linki200 – 200Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei215 – 2151PhosphothreonineBy similarity
Modified residuei224 – 2241N6-acetyllysineBy similarity
Modified residuei265 – 2651PhosphoserineBy similarity

Post-translational modificationi

Sumoylated.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9Z2X1.
MaxQBiQ9Z2X1.
PaxDbiQ9Z2X1.
PeptideAtlasiQ9Z2X1.
PRIDEiQ9Z2X1.

2D gel databases

REPRODUCTION-2DPAGEQ9Z2X1.

PTM databases

iPTMnetiQ9Z2X1.
PhosphoSiteiQ9Z2X1.
SwissPalmiQ9Z2X1.

Expressioni

Gene expression databases

BgeeiENSMUSG00000042079.
ExpressionAtlasiQ9Z2X1. baseline.
GenevisibleiQ9Z2X1. MM.

Interactioni

Subunit structurei

Identified in the spliceosome C complex. Interacts with AGO1, AGO2, TBP and TXNL4/DIM1 (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei16 – 161Interaction with RNABy similarity
Sitei20 – 201Interaction with RNABy similarity
Sitei52 – 521Interaction with RNABy similarity
Sitei75 – 751Interaction with RNABy similarity
Sitei116 – 1161Interaction with RNABy similarity
Sitei120 – 1201Interaction with RNABy similarity
Sitei150 – 1501Interaction with RNABy similarity
Sitei173 – 1731Interaction with RNABy similarity
Sitei294 – 2941Interaction with RNABy similarity
Sitei298 – 2981Interaction with RNABy similarity
Sitei326 – 3261Interaction with RNABy similarity
Sitei349 – 3491Interaction with RNABy similarity

Protein-protein interaction databases

BioGridi221126. 37 interactions.
IntActiQ9Z2X1. 33 interactions.
MINTiMINT-1864385.
STRINGi10090.ENSMUSP00000045048.

Structurei

Secondary structure

1
415
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi3 – 53Combined sources
Beta strandi12 – 176Combined sources
Helixi24 – 307Combined sources
Turni31 – 333Combined sources
Helixi39 – 424Combined sources
Beta strandi43 – 475Combined sources
Beta strandi49 – 513Combined sources
Beta strandi53 – 6311Combined sources
Helixi64 – 707Combined sources
Helixi71 – 733Combined sources
Beta strandi76 – 783Combined sources
Beta strandi81 – 888Combined sources
Helixi90 – 989Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DB1NMR-A1-105[»]
ProteinModelPortaliQ9Z2X1.
SMRiQ9Z2X1. Positions 1-194, 277-381.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Z2X1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 9080RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini111 – 18878RRM 2PROSITE-ProRule annotationAdd
BLAST
Domaini289 – 36678RRM 3PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni81 – 866Interaction with RNABy similarity
Regioni179 – 1846Interaction with RNABy similarity
Regioni355 – 3606Interaction with RNABy similarity

Domaini

The N-terminal RRM domains are responsible for recognizing the G-tract of BCL-X RNA.By similarity

Sequence similaritiesi

Contains 3 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG4211. Eukaryota.
ENOG410Z6M0. LUCA.
GeneTreeiENSGT00760000119102.
HOGENOMiHOG000220896.
HOVERGENiHBG055557.
InParanoidiQ9Z2X1.
KOiK12898.
OMAiFLSECKI.
OrthoDBiEOG091G0CTJ.
PhylomeDBiQ9Z2X1.
TreeFamiTF316157.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR012996. Znf_CHHC.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
PF08080. zf-RNPHF. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 3 hits.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Z2X1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMLGPEGGEG YVVKLRGLPW SCSIEDVQNF LSDCTIHDGV AGVHFIYTRE
60 70 80 90 100
GRQSGEAFVE LESEDDVKLA LKKDRESMGH RYIEVFKSHR TEMDWVLKHS
110 120 130 140 150
GPNSADSAND GFVRLRGLPF GCTKEEIVQF FSGLEIVPNG ITLPVDPEGK
160 170 180 190 200
ITGEAFVQFA SQELAEKALG KHKERIGHRY IEVFKSSQEE VRSYSDPPLK
210 220 230 240 250
FMSVQRPGPY DRPGTARRYI GIVKQAGLDR MRSGAYSAGY GGYEEYSGLS
260 270 280 290 300
DGYGFTTDLF GRDLSYCLSG MYDHRYGDSE FTVQSTTGHC VHMRGLPYKA
310 320 330 340 350
TENDIYNFFS PLNPVRVHIE IGPDGRVTGE ADVEFATHEE AVAAMSKDRA
360 370 380 390 400
NMQHRYIELF LNSTTGASNG AYSSQVMQGM GVSAAQATYS GLESQSVSGC
410
YGAGYSGQNS MGGYD
Length:415
Mass (Da):45,730
Last modified:January 23, 2007 - v3
Checksum:i31E1C9C90A8E051F
GO
Isoform 2 (identifier: Q9Z2X1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-22: MMLGPEGGEGYVVKLRGLPWSC → MW

Show »
Length:395
Mass (Da):43,685
Checksum:i2EC531A33BA024CF
GO

Sequence cautioni

The sequence AAH27003 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH29764 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC36361 differs from that shown. Reason: Erroneous termination at position 416. Translated as stop.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti272 – 2721Y → C in AAH27003 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2222MMLGP…LPWSC → MW in isoform 2. 1 PublicationVSP_021004Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK076478 mRNA. Translation: BAC36361.1. Sequence problems.
BC018185 mRNA. Translation: AAH18185.1.
BC025481 mRNA. Translation: AAH25481.1.
BC027003 mRNA. Translation: AAH27003.1. Different initiation.
BC029163 mRNA. Translation: AAH29163.1.
BC029764 mRNA. Translation: AAH29764.1. Different initiation.
BC033483 mRNA. Translation: AAH33483.1.
BC089313 mRNA. Translation: AAH89313.1.
CCDSiCCDS20467.1. [Q9Z2X1-1]
RefSeqiNP_001159899.1. NM_001166427.1. [Q9Z2X1-1]
NP_001159900.1. NM_001166428.1. [Q9Z2X1-1]
NP_001159901.1. NM_001166429.1. [Q9Z2X1-1]
NP_001159902.1. NM_001166430.1. [Q9Z2X1-1]
NP_001159903.1. NM_001166431.1. [Q9Z2X1-1]
NP_001159904.1. NM_001166432.1. [Q9Z2X1-1]
NP_598595.1. NM_133834.2. [Q9Z2X1-1]
XP_006506892.1. XM_006506829.2. [Q9Z2X1-1]
XP_011239816.1. XM_011241514.1. [Q9Z2X1-1]
UniGeneiMm.422979.
Mm.476420.

Genome annotation databases

EnsembliENSMUST00000035493; ENSMUSP00000045048; ENSMUSG00000042079. [Q9Z2X1-1]
ENSMUST00000163168; ENSMUSP00000130023; ENSMUSG00000042079. [Q9Z2X1-1]
ENSMUST00000167182; ENSMUSP00000126817; ENSMUSG00000042079. [Q9Z2X1-1]
ENSMUST00000180020; ENSMUSP00000137632; ENSMUSG00000042079. [Q9Z2X1-1]
ENSMUST00000180341; ENSMUSP00000136700; ENSMUSG00000042079. [Q9Z2X1-1]
GeneIDi98758.
KEGGimmu:98758.
UCSCiuc009dle.2. mouse. [Q9Z2X1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK076478 mRNA. Translation: BAC36361.1. Sequence problems.
BC018185 mRNA. Translation: AAH18185.1.
BC025481 mRNA. Translation: AAH25481.1.
BC027003 mRNA. Translation: AAH27003.1. Different initiation.
BC029163 mRNA. Translation: AAH29163.1.
BC029764 mRNA. Translation: AAH29764.1. Different initiation.
BC033483 mRNA. Translation: AAH33483.1.
BC089313 mRNA. Translation: AAH89313.1.
CCDSiCCDS20467.1. [Q9Z2X1-1]
RefSeqiNP_001159899.1. NM_001166427.1. [Q9Z2X1-1]
NP_001159900.1. NM_001166428.1. [Q9Z2X1-1]
NP_001159901.1. NM_001166429.1. [Q9Z2X1-1]
NP_001159902.1. NM_001166430.1. [Q9Z2X1-1]
NP_001159903.1. NM_001166431.1. [Q9Z2X1-1]
NP_001159904.1. NM_001166432.1. [Q9Z2X1-1]
NP_598595.1. NM_133834.2. [Q9Z2X1-1]
XP_006506892.1. XM_006506829.2. [Q9Z2X1-1]
XP_011239816.1. XM_011241514.1. [Q9Z2X1-1]
UniGeneiMm.422979.
Mm.476420.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DB1NMR-A1-105[»]
ProteinModelPortaliQ9Z2X1.
SMRiQ9Z2X1. Positions 1-194, 277-381.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi221126. 37 interactions.
IntActiQ9Z2X1. 33 interactions.
MINTiMINT-1864385.
STRINGi10090.ENSMUSP00000045048.

PTM databases

iPTMnetiQ9Z2X1.
PhosphoSiteiQ9Z2X1.
SwissPalmiQ9Z2X1.

2D gel databases

REPRODUCTION-2DPAGEQ9Z2X1.

Proteomic databases

EPDiQ9Z2X1.
MaxQBiQ9Z2X1.
PaxDbiQ9Z2X1.
PeptideAtlasiQ9Z2X1.
PRIDEiQ9Z2X1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035493; ENSMUSP00000045048; ENSMUSG00000042079. [Q9Z2X1-1]
ENSMUST00000163168; ENSMUSP00000130023; ENSMUSG00000042079. [Q9Z2X1-1]
ENSMUST00000167182; ENSMUSP00000126817; ENSMUSG00000042079. [Q9Z2X1-1]
ENSMUST00000180020; ENSMUSP00000137632; ENSMUSG00000042079. [Q9Z2X1-1]
ENSMUST00000180341; ENSMUSP00000136700; ENSMUSG00000042079. [Q9Z2X1-1]
GeneIDi98758.
KEGGimmu:98758.
UCSCiuc009dle.2. mouse. [Q9Z2X1-1]

Organism-specific databases

CTDi3185.
MGIiMGI:2138741. Hnrnpf.

Phylogenomic databases

eggNOGiKOG4211. Eukaryota.
ENOG410Z6M0. LUCA.
GeneTreeiENSGT00760000119102.
HOGENOMiHOG000220896.
HOVERGENiHBG055557.
InParanoidiQ9Z2X1.
KOiK12898.
OMAiFLSECKI.
OrthoDBiEOG091G0CTJ.
PhylomeDBiQ9Z2X1.
TreeFamiTF316157.

Enzyme and pathway databases

ReactomeiR-MMU-6803529. FGFR2 alternative splicing.
R-MMU-72163. mRNA Splicing - Major Pathway.

Miscellaneous databases

ChiTaRSiHnrnpf. mouse.
EvolutionaryTraceiQ9Z2X1.
PROiQ9Z2X1.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000042079.
ExpressionAtlasiQ9Z2X1. baseline.
GenevisibleiQ9Z2X1. MM.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
IPR012996. Znf_CHHC.
[Graphical view]
PfamiPF00076. RRM_1. 2 hits.
PF08080. zf-RNPHF. 1 hit.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 3 hits.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHNRPF_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2X1
Secondary accession number(s): Q5FWK2
, Q8BVU8, Q8K2U9, Q8R0E7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 17, 2006
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 152 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.