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Q9Z2W9

- GRIA3_MOUSE

UniProt

Q9Z2W9 - GRIA3_MOUSE

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Protein
Glutamate receptor 3
Gene
Gria3, Glur3, Kiaa4184
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei474 – 4741Glutamate
Binding sitei509 – 5091Glutamate
Binding sitei731 – 7311Glutamate

GO - Molecular functioni

  1. alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity Source: UniProtKB
  2. extracellular-glutamate-gated ion channel activity Source: RefGenome
  3. protein binding Source: MGI

GO - Biological processi

  1. ion transmembrane transport Source: RefGenome
  2. ionotropic glutamate receptor signaling pathway Source: RefGenome
  3. synaptic transmission, glutamatergic Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate receptor 3
Short name:
GluR-3
Alternative name(s):
AMPA-selective glutamate receptor 3
GluR-C
GluR-K3
Glutamate receptor ionotropic, AMPA 3
Short name:
GluA3
Gene namesi
Name:Gria3
Synonyms:Glur3, Kiaa4184
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:95810. Gria3.

Subcellular locationi

Cell membrane; Multi-pass membrane protein By similarity. Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein By similarity
Note: Interaction with CNIH2 and CNIH3 promotes cell surface expression By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini23 – 546524Extracellular By similarity
Add
BLAST
Transmembranei547 – 56721Helical; By similarity
Add
BLAST
Topological domaini568 – 59629Cytoplasmic By similarity
Add
BLAST
Intramembranei597 – 61216Helical; Pore-forming; By similarity
Add
BLAST
Intramembranei613 – 6153 By similarity
Topological domaini616 – 6216Cytoplasmic By similarity
Transmembranei622 – 64221Helical; By similarity
Add
BLAST
Topological domaini643 – 817175Extracellular By similarity
Add
BLAST
Transmembranei818 – 83821Helical; Name=M4; By similarity
Add
BLAST
Topological domaini839 – 88850Cytoplasmic By similarity
Add
BLAST

GO - Cellular componenti

  1. alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid selective glutamate receptor complex Source: UniProtKB
  2. cell junction Source: UniProtKB-KW
  3. dendrite Source: RefGenome
  4. membrane Source: MGI
  5. postsynaptic membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222 Reviewed prediction
Add
BLAST
Chaini23 – 888866Glutamate receptor 3
PRO_0000042230Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi57 – 571N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi85 ↔ 334 By similarity
Glycosylationi260 – 2601N-linked (GlcNAc...) Reviewed prediction
Glycosylationi374 – 3741N-linked (GlcNAc...) Reviewed prediction
Glycosylationi409 – 4091N-linked (GlcNAc...) Reviewed prediction
Glycosylationi416 – 4161N-linked (GlcNAc...) Reviewed prediction
Lipidationi615 – 6151S-palmitoyl cysteine By similarity
Disulfide bondi744 ↔ 7991 Publication
Lipidationi841 – 8411S-palmitoyl cysteine1 Publication
Modified residuei871 – 8711Phosphotyrosine1 Publication
Modified residuei881 – 8811Phosphotyrosine1 Publication

Post-translational modificationi

Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-615 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-841 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis By similarity.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiQ9Z2W9.
PaxDbiQ9Z2W9.
PRIDEiQ9Z2W9.

PTM databases

PhosphoSiteiQ9Z2W9.

Expressioni

Gene expression databases

ArrayExpressiQ9Z2W9.
BgeeiQ9Z2W9.
CleanExiMM_GRIA3.
GenevestigatoriQ9Z2W9.

Interactioni

Subunit structurei

Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with PRKCABP, GRIP1 and GRIP2 By similarity. Found in a complex with GRIA1, GRIA2, GRIA4, CNIH2, CNIH3, CACNG2, CACNG3, CACNG4, CACNG5, CACNG7 and CACNG8. Interacts with CACNG5 By similarity.1 Publication

Protein-protein interaction databases

BioGridi207340. 2 interactions.
IntActiQ9Z2W9. 4 interactions.
MINTiMINT-4131411.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi662 – 6665
Beta strandi669 – 6768
Helixi680 – 6878
Helixi691 – 70212
Beta strandi708 – 7114
Helixi712 – 72110
Turni722 – 7243
Beta strandi725 – 7317
Helixi732 – 7398
Beta strandi746 – 7505
Beta strandi756 – 7583
Beta strandi761 – 7633
Helixi769 – 78113
Helixi784 – 79310
Turni794 – 7963

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3LSWX-ray1.75A658-799[»]
3LSXX-ray2.01A658-799[»]
ProteinModelPortaliQ9Z2W9.
SMRiQ9Z2W9. Positions 26-843.

Miscellaneous databases

EvolutionaryTraceiQ9Z2W9.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni502 – 5043Glutamate binding
Regioni680 – 6812Glutamate binding

Domaini

The M4 transmembrane segment mediates tetramerization and is required for cell surface expression By similarity.

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG316680.
GeneTreeiENSGT00740000115362.
HOGENOMiHOG000234372.
HOVERGENiHBG051839.
KOiK05199.
OrthoDBiEOG7C2R0J.
TreeFamiTF315232.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu_rcpt_Glu/Gly-bd.
IPR001320. Iontro_glu_rcpt.
IPR001508. NMDA_rcpt.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10613. Lig_chan-Glu_bd. 1 hit.
[Graphical view]
PRINTSiPR00177. NMDARECEPTOR.
SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Flip (identifier: Q9Z2W9-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MGQSVLRAVF FLVLGLLGHS HGGFPNTISI GGLFMRNTVQ EHSAFRFAVQ    50
LYNTNQNTTE KPFHLNYHVD HLDSSNSFSV TNAFCSQFSR GVYAIFGFYD 100
QMSMNTLTSF CGALHTSFVT PSFPTDADVQ FVIQMRPALK GAILSLLGYY 150
KWEKFVYLYD TERGFSILQA IMEAAVQNNW QVTARSVGNI KDIQEFRRII 200
EEMDRRQEKR YLIDCEVERI NTILEQVVIL GKHSRGYHYM LANLGFTDIV 250
LERVMHGGAN ITGFQIVNNE NPMVQQFIQR WVRLDEREFP EAKNAPLKYT 300
SALTHDAILV IAEAFRYLRR QRVDVSRRGS AGDCLANPAV PWSQGIDIER 350
ALKMVQVQGM TGNIQFDTYG RRTNYTIDVY EMKVSGSRKA GYWNEYERFV 400
PFSDQQISND SSSSENRTIV VTTILESPYV MYKKNHEQLE GNERYEGYCV 450
DLAYEIAKHV RIKYKLSIVG DGKYGARDPE TKIWNGMVGE LVYGRADIAV 500
APLTITLVRE EVIDFSKPFM SLGISIMIKK PQKSKPGVFS FLDPLAYEIW 550
MCIVFAYIGV SVVLFLVSRF SPYEWHLEDN NEEPRDPQSP PDPPNEFGIF 600
NSLWFSLGAF MQQGCDISPR SLSGRIVGGV WWFFTLIIIS SYTANLAAFL 650
TVERMVSPIE SAEDLAKQTE IAYGTLDSGS TKEFFRRSKI AVYEKMWSYM 700
KSAEPSVFTK TTADGVARVR KSKGKFAFLL ESTMNEYIEQ RKPCDTMKVG 750
GNLDSKGYGV ATPKGSALRT PVNLAVLKLS EQGILDKLKN KWWYDKGECG 800
AKDSGSKDKT SALSLSNVAG VFYILVGGLG LAMMVALIEF CYKSRAESKR 850
MKLTKNTQNF KPAPATNTQN YATYREGYNV YGTESVKI 888
Length:888
Mass (Da):100,527
Last modified:July 27, 2011 - v2
Checksum:i9F68C5D80E81DC02
GO
Isoform Flop (identifier: Q9Z2W9-2)

Sequence is not available
Length:
Mass (Da):

Sequence cautioni

The sequence BAD90527.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti300 – 3001T → I in BAD90527. 1 Publication
Sequence conflicti769 – 7691R → G in BAA37124. 1 Publication
Sequence conflicti769 – 7691R → G in BAE06153. 1 Publication
Sequence conflicti769 – 7691R → G in AAL90768. 1 Publication
Sequence conflicti769 – 7691R → G in AAL90769. 1 Publication
Sequence conflicti769 – 7691R → G in BAD90527. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB022342 mRNA. Translation: BAA37124.1.
AB079072 mRNA. Translation: BAE06153.1.
AF483494 mRNA. Translation: AAL90768.1.
AF483495 mRNA. Translation: AAL90769.1.
AK220530 mRNA. Translation: BAD90527.1. Different initiation.
CH466570 Genomic DNA. Translation: EDL29035.1.
AL672290, AL672232 Genomic DNA. Translation: CAQ11290.1.
AL672232, AL672290 Genomic DNA. Translation: CAQ12028.1.
BC129855 mRNA. Translation: AAI29856.1.
CCDSiCCDS30097.1. [Q9Z2W9-1]
RefSeqiNP_001268858.1. NM_001281929.1.
NP_001277380.1. NM_001290451.1.
NP_058582.3. NM_016886.4.
XP_006541583.1. XM_006541520.1. [Q9Z2W9-1]
UniGeneiMm.327681.

Genome annotation databases

EnsembliENSMUST00000076349; ENSMUSP00000075687; ENSMUSG00000001986. [Q9Z2W9-1]
GeneIDi53623.
KEGGimmu:53623.
UCSCiuc009tal.2. mouse. [Q9Z2W9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB022342 mRNA. Translation: BAA37124.1 .
AB079072 mRNA. Translation: BAE06153.1 .
AF483494 mRNA. Translation: AAL90768.1 .
AF483495 mRNA. Translation: AAL90769.1 .
AK220530 mRNA. Translation: BAD90527.1 . Different initiation.
CH466570 Genomic DNA. Translation: EDL29035.1 .
AL672290 , AL672232 Genomic DNA. Translation: CAQ11290.1 .
AL672232 , AL672290 Genomic DNA. Translation: CAQ12028.1 .
BC129855 mRNA. Translation: AAI29856.1 .
CCDSi CCDS30097.1. [Q9Z2W9-1 ]
RefSeqi NP_001268858.1. NM_001281929.1.
NP_001277380.1. NM_001290451.1.
NP_058582.3. NM_016886.4.
XP_006541583.1. XM_006541520.1. [Q9Z2W9-1 ]
UniGenei Mm.327681.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
3LSW X-ray 1.75 A 658-799 [» ]
3LSX X-ray 2.01 A 658-799 [» ]
ProteinModelPortali Q9Z2W9.
SMRi Q9Z2W9. Positions 26-843.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 207340. 2 interactions.
IntActi Q9Z2W9. 4 interactions.
MINTi MINT-4131411.

Chemistry

BindingDBi Q9Z2W9.
ChEMBLi CHEMBL2096617.
GuidetoPHARMACOLOGYi 446.

PTM databases

PhosphoSitei Q9Z2W9.

Proteomic databases

MaxQBi Q9Z2W9.
PaxDbi Q9Z2W9.
PRIDEi Q9Z2W9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000076349 ; ENSMUSP00000075687 ; ENSMUSG00000001986 . [Q9Z2W9-1 ]
GeneIDi 53623.
KEGGi mmu:53623.
UCSCi uc009tal.2. mouse. [Q9Z2W9-1 ]

Organism-specific databases

CTDi 2892.
MGIi MGI:95810. Gria3.
Rougei Search...

Phylogenomic databases

eggNOGi NOG316680.
GeneTreei ENSGT00740000115362.
HOGENOMi HOG000234372.
HOVERGENi HBG051839.
KOi K05199.
OrthoDBi EOG7C2R0J.
TreeFami TF315232.

Miscellaneous databases

EvolutionaryTracei Q9Z2W9.
NextBioi 310361.
PROi Q9Z2W9.
SOURCEi Search...

Gene expression databases

ArrayExpressi Q9Z2W9.
Bgeei Q9Z2W9.
CleanExi MM_GRIA3.
Genevestigatori Q9Z2W9.

Family and domain databases

InterProi IPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu_rcpt_Glu/Gly-bd.
IPR001320. Iontro_glu_rcpt.
IPR001508. NMDA_rcpt.
IPR028082. Peripla_BP_I.
[Graphical view ]
Pfami PF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10613. Lig_chan-Glu_bd. 1 hit.
[Graphical view ]
PRINTSi PR00177. NMDARECEPTOR.
SMARTi SM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view ]
SUPFAMi SSF53822. SSF53822. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse glutamate receptor channel alpha3 subunit."
    Sakimura K., Yamazaki M.
    Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
  2. "Expression of AMPA-selective glutamate receptors in mouse spinal cord."
    Doi Y., Nishizawa M., Minami T., Ito S.
    Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ddY.
    Tissue: Spinal cord.
  3. "High-throughput sequence identification of gene coding variants within alcohol-related QTLs."
    Ehringer M.A., Thompson J., Conroy O., Xu Y., Yang F., Canniff J., Beeson M., Gordon L., Bennett B., Johnson T.E., Sikela J.M.
    Mamm. Genome 12:657-663(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ILS and ISS.
  4. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
    Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
    Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  6. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  8. "Differential regulation of AMPA receptor subunit trafficking by palmitoylation of two distinct sites."
    Hayashi T., Rumbaugh G., Huganir R.L.
    Neuron 47:709-723(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMITOYLATION AT CYS-841.
  9. "Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain."
    Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.
    J. Proteome Res. 7:311-318(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-871 AND TYR-881, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain.
  10. "Piracetam defines a new binding site for allosteric modulators of alpha-amino-3-hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA) receptors."
    Ahmed A.H., Oswald R.E.
    J. Med. Chem. 53:2197-2203(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS) OF 658-799 IN COMPLEX WITH GLUTAMATE, SUBUNIT, DISULFIDE BOND.

Entry informationi

Entry nameiGRIA3_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2W9
Secondary accession number(s): A2VDF4, Q5DTJ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 11, 2005
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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