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Q9Z2W8

- GRIA4_MOUSE

UniProt

Q9Z2W8 - GRIA4_MOUSE

Protein

Glutamate receptor 4

Gene

Gria4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 120 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei472 – 4721GlutamateBy similarity
    Binding sitei507 – 5071GlutamateBy similarity
    Binding sitei727 – 7271GlutamateBy similarity

    GO - Molecular functioni

    1. alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity Source: UniProtKB
    2. extracellular-glutamate-gated ion channel activity Source: RefGenome
    3. ionotropic glutamate receptor activity Source: UniProtKB
    4. protein binding Source: MGI

    GO - Biological processi

    1. ionotropic glutamate receptor signaling pathway Source: RefGenome
    2. ion transmembrane transport Source: RefGenome
    3. synaptic transmission, glutamatergic Source: RefGenome

    Keywords - Molecular functioni

    Ion channel, Ligand-gated ion channel, Receptor

    Keywords - Biological processi

    Ion transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate receptor 4
    Short name:
    GluR-4
    Short name:
    GluR4
    Alternative name(s):
    AMPA-selective glutamate receptor 4
    GluR-D
    Glutamate receptor ionotropic, AMPA 4
    Short name:
    GluA4
    Gene namesi
    Name:Gria4
    Synonyms:Glur4
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:95811. Gria4.

    Subcellular locationi

    Cell membrane; Multi-pass membrane protein. Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein. Cell projectiondendrite
    Note: Interaction with CNIH2, CNIH3 and PRKCG promotes cell surface expression.By similarity

    GO - Cellular componenti

    1. alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid selective glutamate receptor complex Source: MGI
    2. cell junction Source: UniProtKB-KW
    3. dendrite Source: RefGenome
    4. kainate selective glutamate receptor complex Source: UniProtKB
    5. postsynaptic membrane Source: RefGenome
    6. synapse Source: MGI

    Keywords - Cellular componenti

    Cell junction, Cell membrane, Cell projection, Membrane, Postsynaptic cell membrane, Synapse

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Chaini21 – 902882Glutamate receptor 4PRO_0000011539Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi56 – 561N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi84 ↔ 331By similarity
    Glycosylationi258 – 2581N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi371 – 3711N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi407 – 4071N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi414 – 4141N-linked (GlcNAc...)Sequence Analysis
    Lipidationi611 – 6111S-palmitoyl cysteineBy similarity
    Disulfide bondi740 ↔ 795By similarity
    Lipidationi837 – 8371S-palmitoyl cysteine1 Publication
    Modified residuei862 – 8621Phosphoserine; by PKC/PRKCGBy similarity

    Post-translational modificationi

    Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-611 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-837 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis By similarity.By similarity
    Phosphorylated at Ser-862 by PRKCG; phosphorylation increases plasma membrane-associated GRI4 expression.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    PaxDbiQ9Z2W8.
    PRIDEiQ9Z2W8.

    PTM databases

    PhosphoSiteiQ9Z2W8.

    Expressioni

    Gene expression databases

    BgeeiQ9Z2W8.
    CleanExiMM_GRIA4.
    GenevestigatoriQ9Z2W8.

    Interactioni

    Subunit structurei

    Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with EPB41L1 via its C-terminus By similarity. Found in a complex with GRIA1, GRIA2, GRIA3, CNIH2, CNIH3, CACNG2, CACNG3, CACNG4, CACNG5, CACNG7 and CACNG8. Interacts with CACNG5 and PRKCG By similarity.By similarity

    Protein-protein interaction databases

    IntActiQ9Z2W8. 2 interactions.
    STRINGi10090.ENSMUSP00000066980.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z2W8.
    SMRiQ9Z2W8. Positions 23-839.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini22 – 544523ExtracellularBy similarityAdd
    BLAST
    Topological domaini566 – 59227CytoplasmicBy similarityAdd
    BLAST
    Topological domaini612 – 6176CytoplasmicBy similarity
    Topological domaini639 – 813175ExtracellularBy similarityAdd
    BLAST
    Topological domaini835 – 90268CytoplasmicBy similarityAdd
    BLAST

    Intramembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Intramembranei593 – 60816Helical; Pore-formingBy similarityAdd
    BLAST
    Intramembranei609 – 6113By similarity

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei545 – 56521HelicalBy similarityAdd
    BLAST
    Transmembranei618 – 63821HelicalBy similarityAdd
    BLAST
    Transmembranei814 – 83421Helical; Name=M4By similarityAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni500 – 5023Glutamate bindingBy similarity
    Regioni676 – 6772Glutamate bindingBy similarity

    Domaini

    The M4 transmembrane segment mediates tetramerization and is required for cell surface expression.By similarity

    Sequence similaritiesi

    Keywords - Domaini

    Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG316680.
    GeneTreeiENSGT00740000115362.
    HOGENOMiHOG000234372.
    HOVERGENiHBG051839.
    InParanoidiQ9Z2W8.
    KOiK05200.

    Family and domain databases

    InterProiIPR001828. ANF_lig-bd_rcpt.
    IPR019594. Glu_rcpt_Glu/Gly-bd.
    IPR001320. Iontro_glu_rcpt.
    IPR001508. NMDA_rcpt.
    IPR028082. Peripla_BP_I.
    [Graphical view]
    PfamiPF01094. ANF_receptor. 1 hit.
    PF00060. Lig_chan. 1 hit.
    PF10613. Lig_chan-Glu_bd. 1 hit.
    [Graphical view]
    PRINTSiPR00177. NMDARECEPTOR.
    SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
    SM00079. PBPe. 1 hit.
    [Graphical view]
    SUPFAMiSSF53822. SSF53822. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    Q9Z2W8-1 [UniParc]FASTAAdd to Basket

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    MRIICRQIVL LFSGFWGLAM GAFPSSVQIG GLFIRNTDQE YTAFRLAIFL    50
    HNTSPNASEA PFNLVPHVDN IETANSFAVT NAFCSQYSRG VFAIFGLYDK 100
    RSVHTLTSFC SALHISLITP SFPTEGESQF VLQLRPSLRG ALLSLLDHYE 150
    WNCFVFLYDT DRGYSILQAI MEKAGQNGWH VSAICVENFN DVSYRQLLEE 200
    LDRRQEKKFV IDCEIERLQN ILEQIVSVGK HVKGYHYIIA NLGFKDISLE 250
    RFIHGGANVT GFQLVDFNTP MVTKLMDRWK KLDQREYPGS ETPPKYTSAL 300
    TYDGVLVMAE TFRSLRRQKI DISRRGNAGD CLANPAAPWG QGIDMERTLK 350
    QVRIQGLTGN VQFDHYGRRV NYTMDVFELK STGPRKVGYW NDMDKLVLIQ 400
    DAPTLGNDTA AIENRTVVVT TIMESPYVMY KKNHEMFEGN DKYEGYCVDL 450
    ASEIAKHIGI KYKIAIVPDG KYGARDADTK IWNGMVGELV YGKAEIAIAP 500
    LTITLVREEV IDFSKPFMSL GISIMIKKPQ KSKPGVFSFL DPLAYEIWMC 550
    IVFAYIGVSV VLFLVSRFSP YEWHTEEPED GKEGPSDQPP NEFGIFNSLW 600
    FSLGAFMQQG CDISPRSLSG RIVGGVWWFF TLIIISSYTA NLAAFLTVER 650
    MVSPIESAED LAKQTEIAYG TLDSGSTKEF FRRSKIAVYE KMWTYMRSAE 700
    PSVFTRTTAE GVARVRKSKG KFAFLLESTM NEYIEQRKPC DTMKVGGNLD 750
    SKGYGVATPK GSSLRTPVNL AVLKLSEAGV LDKLKNKWWY DKGECGPKDS 800
    GSKDKTSALS LSNVAGVFYI LVGGLGLAML VALIEFCYKS RAEAKRMKLT 850
    FSEAIRNKAR LSITGSVGEN GRVLTPDCPK AVHTGTAIRQ SSGLAVIASD 900
    LP 902
    Length:902
    Mass (Da):100,847
    Last modified:July 27, 2011 - v2
    Checksum:i39844E5CE634B841
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti630 – 6301F → G in BAA74538. 1 PublicationCurated
    Sequence conflicti765 – 7673RTP → GNA in BAA74538. 1 PublicationCurated
    Sequence conflicti776 – 7805SEAGV → NEQGL in BAA74538. 1 PublicationCurated
    Sequence conflicti797 – 8015PKDSG → SGGGD in BAA74538. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB022913 mRNA. Translation: BAA74538.1.
    AB079073 mRNA. Translation: BAE06154.1.
    BC060697 mRNA. Translation: AAH60697.1.
    CCDSiCCDS22797.1.
    RefSeqiNP_001106651.1. NM_001113180.1.
    NP_001106652.1. NM_001113181.1.
    NP_062665.3. NM_019691.4.
    UniGeneiMm.209263.

    Genome annotation databases

    EnsembliENSMUST00000063508; ENSMUSP00000066980; ENSMUSG00000025892.
    GeneIDi14802.
    KEGGimmu:14802.
    UCSCiuc009obp.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB022913 mRNA. Translation: BAA74538.1 .
    AB079073 mRNA. Translation: BAE06154.1 .
    BC060697 mRNA. Translation: AAH60697.1 .
    CCDSi CCDS22797.1.
    RefSeqi NP_001106651.1. NM_001113180.1.
    NP_001106652.1. NM_001113181.1.
    NP_062665.3. NM_019691.4.
    UniGenei Mm.209263.

    3D structure databases

    ProteinModelPortali Q9Z2W8.
    SMRi Q9Z2W8. Positions 23-839.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9Z2W8. 2 interactions.
    STRINGi 10090.ENSMUSP00000066980.

    Chemistry

    BindingDBi Q9Z2W8.
    ChEMBLi CHEMBL2096617.
    GuidetoPHARMACOLOGYi 447.

    PTM databases

    PhosphoSitei Q9Z2W8.

    Proteomic databases

    PaxDbi Q9Z2W8.
    PRIDEi Q9Z2W8.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000063508 ; ENSMUSP00000066980 ; ENSMUSG00000025892 .
    GeneIDi 14802.
    KEGGi mmu:14802.
    UCSCi uc009obp.2. mouse.

    Organism-specific databases

    CTDi 2893.
    MGIi MGI:95811. Gria4.

    Phylogenomic databases

    eggNOGi NOG316680.
    GeneTreei ENSGT00740000115362.
    HOGENOMi HOG000234372.
    HOVERGENi HBG051839.
    InParanoidi Q9Z2W8.
    KOi K05200.

    Miscellaneous databases

    ChiTaRSi GRIA4. mouse.
    NextBioi 286965.
    PROi Q9Z2W8.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9Z2W8.
    CleanExi MM_GRIA4.
    Genevestigatori Q9Z2W8.

    Family and domain databases

    InterProi IPR001828. ANF_lig-bd_rcpt.
    IPR019594. Glu_rcpt_Glu/Gly-bd.
    IPR001320. Iontro_glu_rcpt.
    IPR001508. NMDA_rcpt.
    IPR028082. Peripla_BP_I.
    [Graphical view ]
    Pfami PF01094. ANF_receptor. 1 hit.
    PF00060. Lig_chan. 1 hit.
    PF10613. Lig_chan-Glu_bd. 1 hit.
    [Graphical view ]
    PRINTSi PR00177. NMDARECEPTOR.
    SMARTi SM00918. Lig_chan-Glu_bd. 1 hit.
    SM00079. PBPe. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53822. SSF53822. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Mouse glutamate receptor channel alpha4 subunit."
      Sakimura K., Ikeno K.
      Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6.
    2. "Expression of AMPA-selective glutamate receptors in mouse spinal cord."
      Doi Y., Nishizawa M., Minami T., Ito S.
      Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: ddY.
      Tissue: Spinal cord.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Brain.
    4. "Differential regulation of AMPA receptor subunit trafficking by palmitoylation of two distinct sites."
      Hayashi T., Rumbaugh G., Huganir R.L.
      Neuron 47:709-723(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: PALMITOYLATION AT CYS-837.

    Entry informationi

    Entry nameiGRIA4_MOUSE
    AccessioniPrimary (citable) accession number: Q9Z2W8
    Secondary accession number(s): Q6P9M7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 11, 2002
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 120 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3