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Q9Z2W8

- GRIA4_MOUSE

UniProt

Q9Z2W8 - GRIA4_MOUSE

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Protein
Glutamate receptor 4
Gene
Gria4, Glur4
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei472 – 4721Glutamate By similarity
Binding sitei507 – 5071Glutamate By similarity
Binding sitei727 – 7271Glutamate By similarity

GO - Molecular functioni

  1. alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity Source: UniProtKB
  2. extracellular-glutamate-gated ion channel activity Source: RefGenome
  3. ionotropic glutamate receptor activity Source: UniProtKB
  4. protein binding Source: MGI

GO - Biological processi

  1. ion transmembrane transport Source: RefGenome
  2. ionotropic glutamate receptor signaling pathway Source: RefGenome
  3. synaptic transmission, glutamatergic Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate receptor 4
Short name:
GluR-4
Short name:
GluR4
Alternative name(s):
AMPA-selective glutamate receptor 4
GluR-D
Glutamate receptor ionotropic, AMPA 4
Short name:
GluA4
Gene namesi
Name:Gria4
Synonyms:Glur4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:95811. Gria4.

Subcellular locationi

Cell membrane; Multi-pass membrane protein. Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein. Cell projectiondendrite
Note: Interaction with CNIH2, CNIH3 and PRKCG promotes cell surface expression By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 544523Extracellular By similarity
Add
BLAST
Transmembranei545 – 56521Helical; By similarity
Add
BLAST
Topological domaini566 – 59227Cytoplasmic By similarity
Add
BLAST
Intramembranei593 – 60816Helical; Pore-forming; By similarity
Add
BLAST
Intramembranei609 – 6113 By similarity
Topological domaini612 – 6176Cytoplasmic By similarity
Transmembranei618 – 63821Helical; By similarity
Add
BLAST
Topological domaini639 – 813175Extracellular By similarity
Add
BLAST
Transmembranei814 – 83421Helical; Name=M4; By similarity
Add
BLAST
Topological domaini835 – 90268Cytoplasmic By similarity
Add
BLAST

GO - Cellular componenti

  1. alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid selective glutamate receptor complex Source: MGI
  2. cell junction Source: UniProtKB-KW
  3. dendrite Source: RefGenome
  4. kainate selective glutamate receptor complex Source: UniProtKB
  5. postsynaptic membrane Source: RefGenome
  6. synapse Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020 Reviewed prediction
Add
BLAST
Chaini21 – 902882Glutamate receptor 4
PRO_0000011539Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi56 – 561N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi84 ↔ 331 By similarity
Glycosylationi258 – 2581N-linked (GlcNAc...) Reviewed prediction
Glycosylationi371 – 3711N-linked (GlcNAc...) Reviewed prediction
Glycosylationi407 – 4071N-linked (GlcNAc...) Reviewed prediction
Glycosylationi414 – 4141N-linked (GlcNAc...) Reviewed prediction
Lipidationi611 – 6111S-palmitoyl cysteine By similarity
Disulfide bondi740 ↔ 795 By similarity
Lipidationi837 – 8371S-palmitoyl cysteine1 Publication
Modified residuei862 – 8621Phosphoserine; by PKC/PRKCG By similarity

Post-translational modificationi

Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-611 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-837 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis By similarity.1 Publication
Phosphorylated at Ser-862 by PRKCG; phosphorylation increases plasma membrane-associated GRI4 expression By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ9Z2W8.
PRIDEiQ9Z2W8.

PTM databases

PhosphoSiteiQ9Z2W8.

Expressioni

Gene expression databases

BgeeiQ9Z2W8.
CleanExiMM_GRIA4.
GenevestigatoriQ9Z2W8.

Interactioni

Subunit structurei

Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with EPB41L1 via its C-terminus By similarity. Found in a complex with GRIA1, GRIA2, GRIA3, CNIH2, CNIH3, CACNG2, CACNG3, CACNG4, CACNG5, CACNG7 and CACNG8. Interacts with CACNG5 and PRKCG By similarity.

Protein-protein interaction databases

IntActiQ9Z2W8. 2 interactions.
STRINGi10090.ENSMUSP00000066980.

Structurei

3D structure databases

ProteinModelPortaliQ9Z2W8.
SMRiQ9Z2W8. Positions 23-839.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni500 – 5023Glutamate binding By similarity
Regioni676 – 6772Glutamate binding By similarity

Domaini

The M4 transmembrane segment mediates tetramerization and is required for cell surface expression By similarity.

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG316680.
GeneTreeiENSGT00740000115362.
HOGENOMiHOG000234372.
HOVERGENiHBG051839.
InParanoidiQ9Z2W8.
KOiK05200.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu_rcpt_Glu/Gly-bd.
IPR001320. Iontro_glu_rcpt.
IPR001508. NMDA_rcpt.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10613. Lig_chan-Glu_bd. 1 hit.
[Graphical view]
PRINTSiPR00177. NMDARECEPTOR.
SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z2W8-1 [UniParc]FASTAAdd to Basket

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MRIICRQIVL LFSGFWGLAM GAFPSSVQIG GLFIRNTDQE YTAFRLAIFL    50
HNTSPNASEA PFNLVPHVDN IETANSFAVT NAFCSQYSRG VFAIFGLYDK 100
RSVHTLTSFC SALHISLITP SFPTEGESQF VLQLRPSLRG ALLSLLDHYE 150
WNCFVFLYDT DRGYSILQAI MEKAGQNGWH VSAICVENFN DVSYRQLLEE 200
LDRRQEKKFV IDCEIERLQN ILEQIVSVGK HVKGYHYIIA NLGFKDISLE 250
RFIHGGANVT GFQLVDFNTP MVTKLMDRWK KLDQREYPGS ETPPKYTSAL 300
TYDGVLVMAE TFRSLRRQKI DISRRGNAGD CLANPAAPWG QGIDMERTLK 350
QVRIQGLTGN VQFDHYGRRV NYTMDVFELK STGPRKVGYW NDMDKLVLIQ 400
DAPTLGNDTA AIENRTVVVT TIMESPYVMY KKNHEMFEGN DKYEGYCVDL 450
ASEIAKHIGI KYKIAIVPDG KYGARDADTK IWNGMVGELV YGKAEIAIAP 500
LTITLVREEV IDFSKPFMSL GISIMIKKPQ KSKPGVFSFL DPLAYEIWMC 550
IVFAYIGVSV VLFLVSRFSP YEWHTEEPED GKEGPSDQPP NEFGIFNSLW 600
FSLGAFMQQG CDISPRSLSG RIVGGVWWFF TLIIISSYTA NLAAFLTVER 650
MVSPIESAED LAKQTEIAYG TLDSGSTKEF FRRSKIAVYE KMWTYMRSAE 700
PSVFTRTTAE GVARVRKSKG KFAFLLESTM NEYIEQRKPC DTMKVGGNLD 750
SKGYGVATPK GSSLRTPVNL AVLKLSEAGV LDKLKNKWWY DKGECGPKDS 800
GSKDKTSALS LSNVAGVFYI LVGGLGLAML VALIEFCYKS RAEAKRMKLT 850
FSEAIRNKAR LSITGSVGEN GRVLTPDCPK AVHTGTAIRQ SSGLAVIASD 900
LP 902
Length:902
Mass (Da):100,847
Last modified:July 27, 2011 - v2
Checksum:i39844E5CE634B841
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti630 – 6301F → G in BAA74538. 1 Publication
Sequence conflicti765 – 7673RTP → GNA in BAA74538. 1 Publication
Sequence conflicti776 – 7805SEAGV → NEQGL in BAA74538. 1 Publication
Sequence conflicti797 – 8015PKDSG → SGGGD in BAA74538. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB022913 mRNA. Translation: BAA74538.1.
AB079073 mRNA. Translation: BAE06154.1.
BC060697 mRNA. Translation: AAH60697.1.
CCDSiCCDS22797.1.
RefSeqiNP_001106651.1. NM_001113180.1.
NP_001106652.1. NM_001113181.1.
NP_062665.3. NM_019691.4.
UniGeneiMm.209263.

Genome annotation databases

EnsembliENSMUST00000063508; ENSMUSP00000066980; ENSMUSG00000025892.
GeneIDi14802.
KEGGimmu:14802.
UCSCiuc009obp.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB022913 mRNA. Translation: BAA74538.1 .
AB079073 mRNA. Translation: BAE06154.1 .
BC060697 mRNA. Translation: AAH60697.1 .
CCDSi CCDS22797.1.
RefSeqi NP_001106651.1. NM_001113180.1.
NP_001106652.1. NM_001113181.1.
NP_062665.3. NM_019691.4.
UniGenei Mm.209263.

3D structure databases

ProteinModelPortali Q9Z2W8.
SMRi Q9Z2W8. Positions 23-839.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9Z2W8. 2 interactions.
STRINGi 10090.ENSMUSP00000066980.

Chemistry

BindingDBi Q9Z2W8.
ChEMBLi CHEMBL2096617.
GuidetoPHARMACOLOGYi 447.

PTM databases

PhosphoSitei Q9Z2W8.

Proteomic databases

PaxDbi Q9Z2W8.
PRIDEi Q9Z2W8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000063508 ; ENSMUSP00000066980 ; ENSMUSG00000025892 .
GeneIDi 14802.
KEGGi mmu:14802.
UCSCi uc009obp.2. mouse.

Organism-specific databases

CTDi 2893.
MGIi MGI:95811. Gria4.

Phylogenomic databases

eggNOGi NOG316680.
GeneTreei ENSGT00740000115362.
HOGENOMi HOG000234372.
HOVERGENi HBG051839.
InParanoidi Q9Z2W8.
KOi K05200.

Miscellaneous databases

ChiTaRSi GRIA4. mouse.
NextBioi 286965.
PROi Q9Z2W8.
SOURCEi Search...

Gene expression databases

Bgeei Q9Z2W8.
CleanExi MM_GRIA4.
Genevestigatori Q9Z2W8.

Family and domain databases

InterProi IPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu_rcpt_Glu/Gly-bd.
IPR001320. Iontro_glu_rcpt.
IPR001508. NMDA_rcpt.
IPR028082. Peripla_BP_I.
[Graphical view ]
Pfami PF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10613. Lig_chan-Glu_bd. 1 hit.
[Graphical view ]
PRINTSi PR00177. NMDARECEPTOR.
SMARTi SM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view ]
SUPFAMi SSF53822. SSF53822. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse glutamate receptor channel alpha4 subunit."
    Sakimura K., Ikeno K.
    Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
  2. "Expression of AMPA-selective glutamate receptors in mouse spinal cord."
    Doi Y., Nishizawa M., Minami T., Ito S.
    Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ddY.
    Tissue: Spinal cord.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  4. "Differential regulation of AMPA receptor subunit trafficking by palmitoylation of two distinct sites."
    Hayashi T., Rumbaugh G., Huganir R.L.
    Neuron 47:709-723(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMITOYLATION AT CYS-837.

Entry informationi

Entry nameiGRIA4_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2W8
Secondary accession number(s): Q6P9M7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 11, 2002
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 119 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi