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Q9Z2W8

- GRIA4_MOUSE

UniProt

Q9Z2W8 - GRIA4_MOUSE

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Protein

Glutamate receptor 4

Gene

Gria4

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Receptor for glutamate that functions as ligand-gated ion channel in the central nervous system and plays an important role in excitatory synaptic transmission. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate By similarity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei472 – 4721GlutamateBy similarity
Binding sitei507 – 5071GlutamateBy similarity
Binding sitei727 – 7271GlutamateBy similarity

GO - Molecular functioni

  1. alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate selective glutamate receptor activity Source: UniProtKB
  2. extracellular-glutamate-gated ion channel activity Source: RefGenome
  3. ionotropic glutamate receptor activity Source: UniProtKB

GO - Biological processi

  1. ionotropic glutamate receptor signaling pathway Source: RefGenome
  2. ion transmembrane transport Source: RefGenome
  3. synaptic transmission, glutamatergic Source: RefGenome
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate receptor 4
Short name:
GluR-4
Short name:
GluR4
Alternative name(s):
AMPA-selective glutamate receptor 4
GluR-D
Glutamate receptor ionotropic, AMPA 4
Short name:
GluA4
Gene namesi
Name:Gria4
Synonyms:Glur4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 9

Organism-specific databases

MGIiMGI:95811. Gria4.

Subcellular locationi

Cell membrane; Multi-pass membrane protein. Cell junctionsynapsepostsynaptic cell membrane; Multi-pass membrane protein. Cell projectiondendrite
Note: Interaction with CNIH2, CNIH3 and PRKCG promotes cell surface expression.By similarity

GO - Cellular componenti

  1. alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid selective glutamate receptor complex Source: MGI
  2. cell junction Source: UniProtKB-KW
  3. dendrite Source: RefGenome
  4. kainate selective glutamate receptor complex Source: UniProtKB
  5. postsynaptic membrane Source: RefGenome
  6. synapse Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 902882Glutamate receptor 4PRO_0000011539Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi56 – 561N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi84 ↔ 331By similarity
Glycosylationi258 – 2581N-linked (GlcNAc...)Sequence Analysis
Glycosylationi371 – 3711N-linked (GlcNAc...)Sequence Analysis
Glycosylationi407 – 4071N-linked (GlcNAc...)Sequence Analysis
Glycosylationi414 – 4141N-linked (GlcNAc...)Sequence Analysis
Lipidationi611 – 6111S-palmitoyl cysteineBy similarity
Disulfide bondi740 ↔ 795By similarity
Lipidationi837 – 8371S-palmitoyl cysteine1 Publication
Modified residuei862 – 8621Phosphoserine; by PKC/PRKCGBy similarity

Post-translational modificationi

Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-611 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-837 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis By similarity.By similarity
Phosphorylated at Ser-862 by PRKCG; phosphorylation increases plasma membrane-associated GRI4 expression.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

MaxQBiQ9Z2W8.
PaxDbiQ9Z2W8.
PRIDEiQ9Z2W8.

PTM databases

PhosphoSiteiQ9Z2W8.

Expressioni

Gene expression databases

BgeeiQ9Z2W8.
CleanExiMM_GRIA4.
ExpressionAtlasiQ9Z2W8. baseline and differential.
GenevestigatoriQ9Z2W8.

Interactioni

Subunit structurei

Homotetramer or heterotetramer of pore-forming glutamate receptor subunits. Tetramers may be formed by the dimerization of dimers. Interacts with EPB41L1 via its C-terminus By similarity. Found in a complex with GRIA1, GRIA2, GRIA3, CNIH2, CNIH3, CACNG2, CACNG3, CACNG4, CACNG5, CACNG7 and CACNG8. Interacts with CACNG5 and PRKCG By similarity.By similarity

Protein-protein interaction databases

IntActiQ9Z2W8. 2 interactions.
STRINGi10090.ENSMUSP00000066980.

Structurei

3D structure databases

ProteinModelPortaliQ9Z2W8.
SMRiQ9Z2W8. Positions 23-839.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 544523ExtracellularBy similarityAdd
BLAST
Topological domaini566 – 59227CytoplasmicBy similarityAdd
BLAST
Topological domaini612 – 6176CytoplasmicBy similarity
Topological domaini639 – 813175ExtracellularBy similarityAdd
BLAST
Topological domaini835 – 90268CytoplasmicBy similarityAdd
BLAST

Intramembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Intramembranei593 – 60816Helical; Pore-formingBy similarityAdd
BLAST
Intramembranei609 – 6113By similarity

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei545 – 56521HelicalBy similarityAdd
BLAST
Transmembranei618 – 63821HelicalBy similarityAdd
BLAST
Transmembranei814 – 83421Helical; Name=M4By similarityAdd
BLAST

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni500 – 5023Glutamate bindingBy similarity
Regioni676 – 6772Glutamate bindingBy similarity

Domaini

The M4 transmembrane segment mediates tetramerization and is required for cell surface expression.By similarity

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG316680.
GeneTreeiENSGT00760000118920.
HOGENOMiHOG000234372.
HOVERGENiHBG051839.
InParanoidiQ9Z2W8.
KOiK05200.

Family and domain databases

InterProiIPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu_rcpt_Glu/Gly-bd.
IPR001320. Iontro_glu_rcpt.
IPR001508. NMDA_rcpt.
IPR028082. Peripla_BP_I.
[Graphical view]
PfamiPF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10613. Lig_chan-Glu_bd. 1 hit.
[Graphical view]
PRINTSiPR00177. NMDARECEPTOR.
SMARTiSM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view]
SUPFAMiSSF53822. SSF53822. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z2W8-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MRIICRQIVL LFSGFWGLAM GAFPSSVQIG GLFIRNTDQE YTAFRLAIFL
60 70 80 90 100
HNTSPNASEA PFNLVPHVDN IETANSFAVT NAFCSQYSRG VFAIFGLYDK
110 120 130 140 150
RSVHTLTSFC SALHISLITP SFPTEGESQF VLQLRPSLRG ALLSLLDHYE
160 170 180 190 200
WNCFVFLYDT DRGYSILQAI MEKAGQNGWH VSAICVENFN DVSYRQLLEE
210 220 230 240 250
LDRRQEKKFV IDCEIERLQN ILEQIVSVGK HVKGYHYIIA NLGFKDISLE
260 270 280 290 300
RFIHGGANVT GFQLVDFNTP MVTKLMDRWK KLDQREYPGS ETPPKYTSAL
310 320 330 340 350
TYDGVLVMAE TFRSLRRQKI DISRRGNAGD CLANPAAPWG QGIDMERTLK
360 370 380 390 400
QVRIQGLTGN VQFDHYGRRV NYTMDVFELK STGPRKVGYW NDMDKLVLIQ
410 420 430 440 450
DAPTLGNDTA AIENRTVVVT TIMESPYVMY KKNHEMFEGN DKYEGYCVDL
460 470 480 490 500
ASEIAKHIGI KYKIAIVPDG KYGARDADTK IWNGMVGELV YGKAEIAIAP
510 520 530 540 550
LTITLVREEV IDFSKPFMSL GISIMIKKPQ KSKPGVFSFL DPLAYEIWMC
560 570 580 590 600
IVFAYIGVSV VLFLVSRFSP YEWHTEEPED GKEGPSDQPP NEFGIFNSLW
610 620 630 640 650
FSLGAFMQQG CDISPRSLSG RIVGGVWWFF TLIIISSYTA NLAAFLTVER
660 670 680 690 700
MVSPIESAED LAKQTEIAYG TLDSGSTKEF FRRSKIAVYE KMWTYMRSAE
710 720 730 740 750
PSVFTRTTAE GVARVRKSKG KFAFLLESTM NEYIEQRKPC DTMKVGGNLD
760 770 780 790 800
SKGYGVATPK GSSLRTPVNL AVLKLSEAGV LDKLKNKWWY DKGECGPKDS
810 820 830 840 850
GSKDKTSALS LSNVAGVFYI LVGGLGLAML VALIEFCYKS RAEAKRMKLT
860 870 880 890 900
FSEAIRNKAR LSITGSVGEN GRVLTPDCPK AVHTGTAIRQ SSGLAVIASD

LP
Length:902
Mass (Da):100,847
Last modified:July 27, 2011 - v2
Checksum:i39844E5CE634B841
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti630 – 6301F → G in BAA74538. 1 PublicationCurated
Sequence conflicti765 – 7673RTP → GNA in BAA74538. 1 PublicationCurated
Sequence conflicti776 – 7805SEAGV → NEQGL in BAA74538. 1 PublicationCurated
Sequence conflicti797 – 8015PKDSG → SGGGD in BAA74538. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB022913 mRNA. Translation: BAA74538.1.
AB079073 mRNA. Translation: BAE06154.1.
BC060697 mRNA. Translation: AAH60697.1.
CCDSiCCDS22797.1.
RefSeqiNP_001106651.1. NM_001113180.1.
NP_001106652.1. NM_001113181.1.
NP_062665.3. NM_019691.4.
UniGeneiMm.209263.

Genome annotation databases

EnsembliENSMUST00000063508; ENSMUSP00000066980; ENSMUSG00000025892.
GeneIDi14802.
KEGGimmu:14802.
UCSCiuc009obp.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AB022913 mRNA. Translation: BAA74538.1 .
AB079073 mRNA. Translation: BAE06154.1 .
BC060697 mRNA. Translation: AAH60697.1 .
CCDSi CCDS22797.1.
RefSeqi NP_001106651.1. NM_001113180.1.
NP_001106652.1. NM_001113181.1.
NP_062665.3. NM_019691.4.
UniGenei Mm.209263.

3D structure databases

ProteinModelPortali Q9Z2W8.
SMRi Q9Z2W8. Positions 23-839.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi Q9Z2W8. 2 interactions.
STRINGi 10090.ENSMUSP00000066980.

Chemistry

BindingDBi Q9Z2W8.
ChEMBLi CHEMBL2096617.
GuidetoPHARMACOLOGYi 447.

PTM databases

PhosphoSitei Q9Z2W8.

Proteomic databases

MaxQBi Q9Z2W8.
PaxDbi Q9Z2W8.
PRIDEi Q9Z2W8.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000063508 ; ENSMUSP00000066980 ; ENSMUSG00000025892 .
GeneIDi 14802.
KEGGi mmu:14802.
UCSCi uc009obp.2. mouse.

Organism-specific databases

CTDi 2893.
MGIi MGI:95811. Gria4.

Phylogenomic databases

eggNOGi NOG316680.
GeneTreei ENSGT00760000118920.
HOGENOMi HOG000234372.
HOVERGENi HBG051839.
InParanoidi Q9Z2W8.
KOi K05200.

Miscellaneous databases

ChiTaRSi GRIA4. mouse.
NextBioi 286965.
PROi Q9Z2W8.
SOURCEi Search...

Gene expression databases

Bgeei Q9Z2W8.
CleanExi MM_GRIA4.
ExpressionAtlasi Q9Z2W8. baseline and differential.
Genevestigatori Q9Z2W8.

Family and domain databases

InterProi IPR001828. ANF_lig-bd_rcpt.
IPR019594. Glu_rcpt_Glu/Gly-bd.
IPR001320. Iontro_glu_rcpt.
IPR001508. NMDA_rcpt.
IPR028082. Peripla_BP_I.
[Graphical view ]
Pfami PF01094. ANF_receptor. 1 hit.
PF00060. Lig_chan. 1 hit.
PF10613. Lig_chan-Glu_bd. 1 hit.
[Graphical view ]
PRINTSi PR00177. NMDARECEPTOR.
SMARTi SM00918. Lig_chan-Glu_bd. 1 hit.
SM00079. PBPe. 1 hit.
[Graphical view ]
SUPFAMi SSF53822. SSF53822. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse glutamate receptor channel alpha4 subunit."
    Sakimura K., Ikeno K.
    Submitted (JAN-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
  2. "Expression of AMPA-selective glutamate receptors in mouse spinal cord."
    Doi Y., Nishizawa M., Minami T., Ito S.
    Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: ddY.
    Tissue: Spinal cord.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6.
    Tissue: Brain.
  4. "Differential regulation of AMPA receptor subunit trafficking by palmitoylation of two distinct sites."
    Hayashi T., Rumbaugh G., Huganir R.L.
    Neuron 47:709-723(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMITOYLATION AT CYS-837.

Entry informationi

Entry nameiGRIA4_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2W8
Secondary accession number(s): Q6P9M7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 11, 2002
Last sequence update: July 27, 2011
Last modified: October 29, 2014
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds AMPA (quisqualate) > glutamate > kainate.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3