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Q9Z2W1

- STK25_MOUSE

UniProt

Q9Z2W1 - STK25_MOUSE

Protein

Serine/threonine-protein kinase 25

Gene

Stk25

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Oxidant stress-activated serine/threonine kinase that may play a role in the response to environmental stress. Targets to the Golgi apparatus where it appears to regulate protein transport events, cell adhesion, and polarity complexes important for cell migration By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Interaction with Golgi matrix protein GOLGA2 leads to autophosphorylation on Thr-174, possibly as a consequence of stabilization of dimer formation. The C-terminal non-catalytic region inhibits the kinase activity By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei49 – 491ATPPROSITE-ProRule annotation
    Active sitei140 – 1401Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi26 – 349ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. protein binding Source: MGI
    4. protein serine/threonine kinase activity Source: UniProtKB-KW

    GO - Biological processi

    1. establishment or maintenance of cell polarity Source: MGI
    2. Golgi localization Source: MGI
    3. positive regulation of axonogenesis Source: MGI
    4. protein phosphorylation Source: MGI

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine-protein kinase 25 (EC:2.7.11.1)
    Alternative name(s):
    Ste20-like kinase
    Sterile 20/oxidant stress-response kinase 1
    Short name:
    SOK-1
    Short name:
    Ste20/oxidant stress response kinase 1
    Gene namesi
    Name:Stk25
    Synonyms:Sok1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:1891699. Stk25.

    Subcellular locationi

    Cytoplasm By similarity. Golgi apparatus By similarity
    Note: Localizes to the Golgi apparatus.By similarity

    GO - Cellular componenti

    1. Golgi apparatus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Golgi apparatus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 426426Serine/threonine-protein kinase 25PRO_0000086714Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei174 – 1741Phosphothreonine; by autocatalysis

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ9Z2W1.
    PaxDbiQ9Z2W1.
    PRIDEiQ9Z2W1.

    PTM databases

    PhosphoSiteiQ9Z2W1.

    Expressioni

    Gene expression databases

    ArrayExpressiQ9Z2W1.
    BgeeiQ9Z2W1.
    CleanExiMM_STK25.
    GenevestigatoriQ9Z2W1.

    Interactioni

    Subunit structurei

    Homodimer. Interacts with CTTNBP2NL.By similarity

    Protein-protein interaction databases

    IntActiQ9Z2W1. 4 interactions.
    MINTiMINT-4135919.
    STRINGi10090.ENSMUSP00000027498.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z2W1.
    SMRiQ9Z2W1. Positions 3-292, 355-418.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini20 – 270251Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00640000091192.
    HOGENOMiHOG000234203.
    HOVERGENiHBG108518.
    InParanoidiQ6IR17.
    KOiK08838.
    OMAiFSHNRNH.
    OrthoDBiEOG77T14Q.
    TreeFamiTF354217.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9Z2W1-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAHLRGFAHQ HSRVDPEELF TKLDRIGKGS FGEVYKGIDN HTKEVVAIKI    50
    IDLEEAEDEI EDIQQEITVL SQCDSPYITR YFGSYLKSTK LWIIMEYLGG 100
    GSALDLLKPG PLEETYIATI LREILKGLDY LHSERKIHRD IKAANVLLSE 150
    QGDVKLADFG VAGQLTDTQI KRNTFVGTPF WMAPEVIKQS AYDFKADIWS 200
    LGITAIELAK GEPPNSDLHP MRVLFLIPKN NPPTLEGHHS KPFKEFVEAC 250
    LNKDPRFRPT AKELLKHKFI TRYTKKTSFL TELIDRYKRW KSEGHGEESS 300
    SEDSDIDGEA EDGEQGPIWT FPPTIRPSPH SKLHKGTALH SSQKPAEPIK 350
    RQPRSQCLST LVRPVFGELK EKHKQSGGSV GALEELENAF SLAEESCPGI 400
    SDKLMVHLVE RVQRFSHSRN HLTSTR 426
    Length:426
    Mass (Da):48,158
    Last modified:July 27, 2011 - v2
    Checksum:i70B191AB55E26337
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti156 – 1561L → M in AAD01208. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF004934 mRNA. Translation: AAD01208.1.
    AK148041 mRNA. Translation: BAE28307.1.
    CH466520 Genomic DNA. Translation: EDL39941.1.
    CH466520 Genomic DNA. Translation: EDL39942.1.
    BC071218 mRNA. Translation: AAH71218.1.
    CCDSiCCDS15192.1.
    RefSeqiNP_067512.3. NM_021537.3.
    XP_006529831.1. XM_006529768.1.
    UniGeneiMm.28761.

    Genome annotation databases

    EnsembliENSMUST00000027498; ENSMUSP00000027498; ENSMUSG00000026277.
    GeneIDi59041.
    KEGGimmu:59041.
    UCSCiuc007ced.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF004934 mRNA. Translation: AAD01208.1 .
    AK148041 mRNA. Translation: BAE28307.1 .
    CH466520 Genomic DNA. Translation: EDL39941.1 .
    CH466520 Genomic DNA. Translation: EDL39942.1 .
    BC071218 mRNA. Translation: AAH71218.1 .
    CCDSi CCDS15192.1.
    RefSeqi NP_067512.3. NM_021537.3.
    XP_006529831.1. XM_006529768.1.
    UniGenei Mm.28761.

    3D structure databases

    ProteinModelPortali Q9Z2W1.
    SMRi Q9Z2W1. Positions 3-292, 355-418.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9Z2W1. 4 interactions.
    MINTi MINT-4135919.
    STRINGi 10090.ENSMUSP00000027498.

    PTM databases

    PhosphoSitei Q9Z2W1.

    Proteomic databases

    MaxQBi Q9Z2W1.
    PaxDbi Q9Z2W1.
    PRIDEi Q9Z2W1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000027498 ; ENSMUSP00000027498 ; ENSMUSG00000026277 .
    GeneIDi 59041.
    KEGGi mmu:59041.
    UCSCi uc007ced.1. mouse.

    Organism-specific databases

    CTDi 10494.
    MGIi MGI:1891699. Stk25.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00640000091192.
    HOGENOMi HOG000234203.
    HOVERGENi HBG108518.
    InParanoidi Q6IR17.
    KOi K08838.
    OMAi FSHNRNH.
    OrthoDBi EOG77T14Q.
    TreeFami TF354217.

    Miscellaneous databases

    ChiTaRSi STK25. mouse.
    NextBioi 314650.
    PROi Q9Z2W1.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9Z2W1.
    Bgeei Q9Z2W1.
    CleanExi MM_STK25.
    Genevestigatori Q9Z2W1.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genetic mapping of human and mouse PAK genes."
      Melnick M.B.
      Submitted (MAY-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6J.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Czech II.
      Tissue: Mammary tumor.

    Entry informationi

    Entry nameiSTK25_MOUSE
    AccessioniPrimary (citable) accession number: Q9Z2W1
    Secondary accession number(s): Q6IR17
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 117 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3