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Q9Z2W0

- DNPEP_MOUSE

UniProt

Q9Z2W0 - DNPEP_MOUSE

Protein

Aspartyl aminopeptidase

Gene

Dnpep

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Aminopeptidase with specificity towards an acidic amino acid at the N-terminus. Likely to play an important role in intracellular protein and peptide metabolism By similarity.By similarity

    Catalytic activityi

    Release of an N-terminal aspartate or glutamate from a peptide, with a preference for aspartate.

    Cofactori

    Binds 2 zinc ions per subunit.By similarity

    Enzyme regulationi

    One of the zinc ions is readily exchangeable with other divalent cations such as manganese, which strongly stimulates the enzymatic activity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi92 – 921Zinc 1By similarity
    Binding sitei168 – 1681SubstrateBy similarity
    Metal bindingi262 – 2621Zinc 1By similarity
    Metal bindingi262 – 2621Zinc 2By similarity
    Binding sitei299 – 2991SubstrateBy similarity
    Metal bindingi300 – 3001Zinc 2By similarity
    Metal bindingi344 – 3441Zinc 1By similarity
    Binding sitei344 – 3441SubstrateBy similarity
    Binding sitei347 – 3471SubstrateBy similarity
    Binding sitei372 – 3721SubstrateBy similarity
    Binding sitei379 – 3791SubstrateBy similarity
    Metal bindingi438 – 4381Zinc 2By similarity

    GO - Molecular functioni

    1. aminopeptidase activity Source: UniProtKB-KW
    2. metallopeptidase activity Source: UniProtKB-KW
    3. zinc ion binding Source: InterPro

    Keywords - Molecular functioni

    Aminopeptidase, Hydrolase, Metalloprotease, Protease

    Keywords - Ligandi

    Metal-binding, Zinc

    Protein family/group databases

    MEROPSiM18.002.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Aspartyl aminopeptidase (EC:3.4.11.21)
    Gene namesi
    Name:Dnpep
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:1278328. Dnpep.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 473473Aspartyl aminopeptidasePRO_0000173452Add
    BLAST

    Proteomic databases

    MaxQBiQ9Z2W0.
    PaxDbiQ9Z2W0.
    PRIDEiQ9Z2W0.

    2D gel databases

    REPRODUCTION-2DPAGEQ9Z2W0.

    PTM databases

    PhosphoSiteiQ9Z2W0.

    Expressioni

    Tissue specificityi

    Ubiquitous.

    Gene expression databases

    ArrayExpressiQ9Z2W0.
    BgeeiQ9Z2W0.
    CleanExiMM_DNPEP.
    GenevestigatoriQ9Z2W0.

    Interactioni

    Subunit structurei

    Tetrahedron-shaped homododecamer built from six homodimers.By similarity

    Protein-protein interaction databases

    IntActiQ9Z2W0. 2 interactions.
    MINTiMINT-4093364.
    STRINGi10090.ENSMUSP00000070821.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z2W0.
    SMRiQ9Z2W0. Positions 5-473.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase M18 family.Curated

    Phylogenomic databases

    eggNOGiCOG1362.
    GeneTreeiENSGT00390000003164.
    HOGENOMiHOG000253244.
    HOVERGENiHBG051386.
    InParanoidiQ56A00.
    KOiK01267.
    OMAiIVKCPTS.
    OrthoDBiEOG789CB1.
    TreeFamiTF300487.

    Family and domain databases

    Gene3Di2.30.250.10. 1 hit.
    InterProiIPR001948. Peptidase_M18.
    IPR023358. Peptidase_M18_dom2.
    [Graphical view]
    PfamiPF02127. Peptidase_M18. 1 hit.
    [Graphical view]
    PRINTSiPR00932. AMINO1PTASE.

    Sequencei

    Sequence statusi: Complete.

    Q9Z2W0-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAMNGRARKE AIQATARELL KFVNRSPSPF HVVAECRSRL LQAGFRELKE    50
    TEGWDIVPEN KYFLTRNSSS IIAFAVGGQY VPGNGFSLIG AHTDSPCLRV 100
    KRKSRRSQVG YHQVGVETYG GGIWSTWFDR DLTLAGRVII KCPTSGRLEQ 150
    RLVHIERPIL RIPHLAIHLQ RNINENFGPN TEIHLVPILA TAVQEELEKG 200
    TPEPGPLGAT DERHHSVLMS LLCTHLGLSP DSIMEMELCL ADTQPAVLGG 250
    AYEEFIFAPR LDNLHSCFCA LQALIDSCAS PASLARDPHV RMVTLYDNEE 300
    VGSESAQGAQ SLLTELILRR ISASPQRLTA FEEAIPKSFM ISADMAHAVH 350
    PNYSDKHEEN HRPLFHKGPV IKVNSKQRYA SNAVSESMIR EVAGQVGVPL 400
    QDLMVRNDSP CGTTIGPILA SRLGLRVLDL GSPQLAMHSI RETACTTGVL 450
    QTLTLFKGFF ELFPSVSRNL LVD 473
    Length:473
    Mass (Da):52,207
    Last modified:July 27, 2011 - v2
    Checksum:i0350CC27C4A5AC8E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti61 – 611K → N in AAD01212. (PubMed:9632644)Curated
    Sequence conflicti364 – 3641L → S in AAD01212. (PubMed:9632644)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF005051 mRNA. Translation: AAD01212.1.
    AK166276 mRNA. Translation: BAE38675.1.
    CH466548 Genomic DNA. Translation: EDL00396.1.
    CH466548 Genomic DNA. Translation: EDL00398.1.
    CH466548 Genomic DNA. Translation: EDL00400.1.
    CH466548 Genomic DNA. Translation: EDL00401.1.
    BC092232 mRNA. Translation: AAH92232.1.
    CCDSiCCDS15070.1.
    RefSeqiNP_001104301.1. NM_001110831.1.
    NP_058574.3. NM_016878.4.
    XP_006496468.1. XM_006496405.1.
    XP_006496469.1. XM_006496406.1.
    UniGeneiMm.24680.

    Genome annotation databases

    EnsembliENSMUST00000066668; ENSMUSP00000070821; ENSMUSG00000026209.
    ENSMUST00000113605; ENSMUSP00000109235; ENSMUSG00000026209.
    GeneIDi13437.
    KEGGimmu:13437.
    UCSCiuc007bot.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF005051 mRNA. Translation: AAD01212.1 .
    AK166276 mRNA. Translation: BAE38675.1 .
    CH466548 Genomic DNA. Translation: EDL00396.1 .
    CH466548 Genomic DNA. Translation: EDL00398.1 .
    CH466548 Genomic DNA. Translation: EDL00400.1 .
    CH466548 Genomic DNA. Translation: EDL00401.1 .
    BC092232 mRNA. Translation: AAH92232.1 .
    CCDSi CCDS15070.1.
    RefSeqi NP_001104301.1. NM_001110831.1.
    NP_058574.3. NM_016878.4.
    XP_006496468.1. XM_006496405.1.
    XP_006496469.1. XM_006496406.1.
    UniGenei Mm.24680.

    3D structure databases

    ProteinModelPortali Q9Z2W0.
    SMRi Q9Z2W0. Positions 5-473.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9Z2W0. 2 interactions.
    MINTi MINT-4093364.
    STRINGi 10090.ENSMUSP00000070821.

    Protein family/group databases

    MEROPSi M18.002.

    PTM databases

    PhosphoSitei Q9Z2W0.

    2D gel databases

    REPRODUCTION-2DPAGE Q9Z2W0.

    Proteomic databases

    MaxQBi Q9Z2W0.
    PaxDbi Q9Z2W0.
    PRIDEi Q9Z2W0.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000066668 ; ENSMUSP00000070821 ; ENSMUSG00000026209 .
    ENSMUST00000113605 ; ENSMUSP00000109235 ; ENSMUSG00000026209 .
    GeneIDi 13437.
    KEGGi mmu:13437.
    UCSCi uc007bot.2. mouse.

    Organism-specific databases

    CTDi 23549.
    MGIi MGI:1278328. Dnpep.

    Phylogenomic databases

    eggNOGi COG1362.
    GeneTreei ENSGT00390000003164.
    HOGENOMi HOG000253244.
    HOVERGENi HBG051386.
    InParanoidi Q56A00.
    KOi K01267.
    OMAi IVKCPTS.
    OrthoDBi EOG789CB1.
    TreeFami TF300487.

    Miscellaneous databases

    ChiTaRSi DNPEP. mouse.
    NextBioi 283883.
    PROi Q9Z2W0.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9Z2W0.
    Bgeei Q9Z2W0.
    CleanExi MM_DNPEP.
    Genevestigatori Q9Z2W0.

    Family and domain databases

    Gene3Di 2.30.250.10. 1 hit.
    InterProi IPR001948. Peptidase_M18.
    IPR023358. Peptidase_M18_dom2.
    [Graphical view ]
    Pfami PF02127. Peptidase_M18. 1 hit.
    [Graphical view ]
    PRINTSi PR00932. AMINO1PTASE.
    ProtoNeti Search...

    Publicationsi

    1. "Purification, characterization, and cloning of a cytosolic aspartyl aminopeptidase."
      Wilk S., Wilk E., Magnusson R.P.
      J. Biol. Chem. 273:15961-15970(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Mammary gland.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6.
      Tissue: Eye.

    Entry informationi

    Entry nameiDNPEP_MOUSE
    AccessioniPrimary (citable) accession number: Q9Z2W0
    Secondary accession number(s): Q56A00
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 24, 2001
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 99 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3