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Q9Z2V4

- PCKGC_MOUSE

UniProt

Q9Z2V4 - PCKGC_MOUSE

Protein

Phosphoenolpyruvate carboxykinase, cytosolic [GTP]

Gene

Pck1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.By similarity

    Catalytic activityi

    GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.

    Cofactori

    Binds 1 manganese ion per subunit.By similarity

    Enzyme regulationi

    Enzyme activity is enhanced by acetylation.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei87 – 871SubstrateBy similarity
    Binding sitei237 – 2371Substrate; via amide nitrogenBy similarity
    Metal bindingi244 – 2441ManganeseBy similarity
    Binding sitei244 – 2441SubstrateBy similarity
    Metal bindingi264 – 2641Manganese; via tele nitrogenBy similarity
    Binding sitei286 – 2861SubstrateBy similarity
    Active sitei288 – 2881By similarity
    Metal bindingi311 – 3111ManganeseBy similarity
    Binding sitei405 – 4051GTPBy similarity
    Binding sitei436 – 4361GTPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi287 – 2926GTPBy similarity
    Nucleotide bindingi530 – 5334GTPBy similarity

    GO - Molecular functioni

    1. carboxylic acid binding Source: Ensembl
    2. GDP binding Source: Ensembl
    3. GTP binding Source: UniProtKB-KW
    4. magnesium ion binding Source: Ensembl
    5. manganese ion binding Source: Ensembl
    6. phosphoenolpyruvate carboxykinase (GTP) activity Source: UniProtKB-EC
    7. phosphoenolpyruvate carboxykinase activity Source: MGI

    GO - Biological processi

    1. gluconeogenesis Source: MGI
    2. glucose homeostasis Source: Ensembl
    3. glycerol biosynthetic process from pyruvate Source: MGI
    4. internal protein amino acid acetylation Source: UniProtKB
    5. lipid metabolic process Source: MGI
    6. oxaloacetate metabolic process Source: Ensembl
    7. response to activity Source: Ensembl
    8. response to insulin Source: Ensembl

    Keywords - Molecular functioni

    Decarboxylase, Lyase

    Keywords - Biological processi

    Gluconeogenesis

    Keywords - Ligandi

    GTP-binding, Manganese, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_196553. Abacavir metabolism.
    SABIO-RKQ9Z2V4.
    UniPathwayiUPA00138.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoenolpyruvate carboxykinase, cytosolic [GTP] (EC:4.1.1.32)
    Short name:
    PEPCK-C
    Gene namesi
    Name:Pck1
    Synonyms:Pepck
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 2

    Organism-specific databases

    MGIiMGI:97501. Pck1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 622622Phosphoenolpyruvate carboxykinase, cytosolic [GTP]PRO_0000103628Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei70 – 701N6-acetyllysine; by p300/EP300By similarity
    Modified residuei71 – 711N6-acetyllysine; by p300/EP300By similarity

    Post-translational modificationi

    Lysine acetylation by p300/EP300 is increased on high glucose conditions and promotes ubiquitination by UBR5, acetylation is enhanced in the presence of BAG6. Deacetylated by SIRT2 By similarity.By similarity
    Ubiquitination by UBR5 leads to proteasomal degradation.By similarity

    Keywords - PTMi

    Acetylation, Ubl conjugation

    Proteomic databases

    MaxQBiQ9Z2V4.
    PaxDbiQ9Z2V4.
    PRIDEiQ9Z2V4.

    PTM databases

    PhosphoSiteiQ9Z2V4.

    Expressioni

    Inductioni

    Regulated by glucocortinoids and insulin.1 Publication

    Gene expression databases

    BgeeiQ9Z2V4.
    CleanExiMM_PCK1.
    GenevestigatoriQ9Z2V4.

    Interactioni

    Subunit structurei

    Monomer.

    Protein-protein interaction databases

    IntActiQ9Z2V4. 1 interaction.
    MINTiMINT-1869570.
    STRINGi10090.ENSMUSP00000029017.

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z2V4.
    SMRiQ9Z2V4. Positions 1-622.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni403 – 4053Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1274.
    GeneTreeiENSGT00390000001912.
    HOGENOMiHOG000191700.
    HOVERGENiHBG053651.
    InParanoidiQ9Z2V4.
    KOiK01596.
    OMAiPDHIHIC.
    OrthoDBiEOG7KSX81.
    PhylomeDBiQ9Z2V4.
    TreeFamiTF314402.

    Family and domain databases

    Gene3Di3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPiMF_00452. PEPCK_GTP.
    InterProiIPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view]
    PANTHERiPTHR11561. PTHR11561. 1 hit.
    PfamiPF00821. PEPCK. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMiSSF68923. SSF68923. 1 hit.
    PROSITEiPS00505. PEPCK_GTP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q9Z2V4-1 [UniParc]FASTAAdd to Basket

    « Hide

    MPPQLHNGLD FSAKVIQGSL DSLPQAVRKF VEGNAQLCQP EYIHICDGSE    50
    EEYGQLLAHM QEEGVIRKLK KYDNCWLALT DPRDVARIES KTVIITQEQR 100
    DTVPIPKTGL SQLGRWMSEE DFEKAFNARF PGCMKGRTMY VIPFSMGPLG 150
    SPLAKIGIEL TDSPYVVASM RIMTRMGISV LEALGDGEFI KCLHSVGCPL 200
    PLKKPLVNNW ACNPELTLIA HLPDRREIIS FGSGYGGNSL LGKKCFALRI 250
    ASRLAKEEGW LAEHMLILGI TNPEGKKKYL AAAFPSACGK TNLAMMNPSL 300
    PGWKVECVGD DIAWMKFDAQ GNLRAINPEN GFFGVAPGTS VKTNPNAIKT 350
    IQKNTIFTNV AETSDGGVYW EGIDEPLAPG VTITSWKNKE WRPQDAEPCA 400
    HPNSRFCTPA SQCPIIDPAW ESPEGVPIEG IIFGGRRPEG VPLVYEALSW 450
    QHGVFVGAAM RSEATAAAEH KGKIIMHDPF AMRPFFGYNF GKYLAHWLSM 500
    AHRPAAKLPK IFHVNWFRKD KDGKFLWPGF GENSRVLEWM FGRIEGEDSA 550
    KLTPIGYIPK ENALNLKGLG GVNVEELFGI SKEFWEKEVE EIDRYLEDQV 600
    NTDLPYEIER ELRALKQRIS QM 622
    Length:622
    Mass (Da):69,355
    Last modified:May 1, 1999 - v1
    Checksum:i9D94738B4A0EE196
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF009605 Genomic DNA. Translation: AAD01427.1.
    AK028046 mRNA. Translation: BAC25718.1.
    AK133496 mRNA. Translation: BAE21687.1.
    AK149525 mRNA. Translation: BAE28938.1.
    CCDSiCCDS17141.1.
    RefSeqiNP_035174.1. NM_011044.2.
    UniGeneiMm.266867.

    Genome annotation databases

    EnsembliENSMUST00000029017; ENSMUSP00000029017; ENSMUSG00000027513.
    GeneIDi18534.
    KEGGimmu:18534.
    UCSCiuc008odm.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF009605 Genomic DNA. Translation: AAD01427.1 .
    AK028046 mRNA. Translation: BAC25718.1 .
    AK133496 mRNA. Translation: BAE21687.1 .
    AK149525 mRNA. Translation: BAE28938.1 .
    CCDSi CCDS17141.1.
    RefSeqi NP_035174.1. NM_011044.2.
    UniGenei Mm.266867.

    3D structure databases

    ProteinModelPortali Q9Z2V4.
    SMRi Q9Z2V4. Positions 1-622.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi Q9Z2V4. 1 interaction.
    MINTi MINT-1869570.
    STRINGi 10090.ENSMUSP00000029017.

    PTM databases

    PhosphoSitei Q9Z2V4.

    Proteomic databases

    MaxQBi Q9Z2V4.
    PaxDbi Q9Z2V4.
    PRIDEi Q9Z2V4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000029017 ; ENSMUSP00000029017 ; ENSMUSG00000027513 .
    GeneIDi 18534.
    KEGGi mmu:18534.
    UCSCi uc008odm.1. mouse.

    Organism-specific databases

    CTDi 5105.
    MGIi MGI:97501. Pck1.

    Phylogenomic databases

    eggNOGi COG1274.
    GeneTreei ENSGT00390000001912.
    HOGENOMi HOG000191700.
    HOVERGENi HBG053651.
    InParanoidi Q9Z2V4.
    KOi K01596.
    OMAi PDHIHIC.
    OrthoDBi EOG7KSX81.
    PhylomeDBi Q9Z2V4.
    TreeFami TF314402.

    Enzyme and pathway databases

    UniPathwayi UPA00138 .
    Reactomei REACT_196553. Abacavir metabolism.
    SABIO-RK Q9Z2V4.

    Miscellaneous databases

    ChiTaRSi PCK1. mouse.
    NextBioi 294300.
    PROi Q9Z2V4.
    SOURCEi Search...

    Gene expression databases

    Bgeei Q9Z2V4.
    CleanExi MM_PCK1.
    Genevestigatori Q9Z2V4.

    Family and domain databases

    Gene3Di 3.40.449.10. 1 hit.
    3.90.228.20. 2 hits.
    HAMAPi MF_00452. PEPCK_GTP.
    InterProi IPR018091. PEP_carboxykin_GTP_CS.
    IPR013035. PEP_carboxykinase_C.
    IPR008209. PEP_carboxykinase_GTP.
    IPR008210. PEP_carboxykinase_N.
    [Graphical view ]
    PANTHERi PTHR11561. PTHR11561. 1 hit.
    Pfami PF00821. PEPCK. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001348. PEP_carboxykinase_GTP. 1 hit.
    SUPFAMi SSF68923. SSF68923. 1 hit.
    PROSITEi PS00505. PEPCK_GTP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of the mouse cytosolic phosphoenolpyruvate carboxykinase (GTP) gene: evidence for tissue-specific hypersensitive sites."
      Williams C.P., Postic C., Robin D., Robin P., Parrinello J., Shelton K., Printz R.L., Magnuson M.A., Granner D.K., Forest C., Chalkley R.
      Mol. Cell. Endocrinol. 148:67-77(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 129/Sv.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Liver and Ovary.
    3. "Glucocorticoids regulate transcription of the gene for phosphoenolpyruvate carboxykinase in the liver via an extended glucocorticoid regulatory unit."
      Cassuto H., Kochan K., Chakravarty K., Cohen H., Blum B., Olswang Y., Hakimi P., Xu C., Massillon D., Hanson R.W., Reshef L.
      J. Biol. Chem. 280:33873-33884(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INDUCTION.

    Entry informationi

    Entry nameiPCKGC_MOUSE
    AccessioniPrimary (citable) accession number: Q9Z2V4
    Secondary accession number(s): Q3UEH3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 26, 2001
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3