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Protein

Phosphoenolpyruvate carboxykinase, cytosolic [GTP]

Gene

Pck1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP), the rate-limiting step in the metabolic pathway that produces glucose from lactate and other precursors derived from the citric acid cycle.By similarity

Miscellaneous

In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one.

Catalytic activityi

GTP + oxaloacetate = GDP + phosphoenolpyruvate + CO2.By similarity

Cofactori

Mn2+By similarityNote: Binds 1 Mn2+ ion per subunit.By similarity

Enzyme regulationi

Enzyme activity is enhanced by acetylation.By similarity

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.By similarity
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei87SubstrateBy similarity1
Metal bindingi244ManganeseBy similarity1
Metal bindingi264Manganese; via tele nitrogenBy similarity1
Binding sitei286SubstrateBy similarity1
Active sitei288By similarity1
Metal bindingi311ManganeseBy similarity1
Binding sitei405GTPBy similarity1
Binding sitei436GTPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi287 – 292GTPBy similarity6
Nucleotide bindingi530 – 533GTPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Lyase
Biological processGluconeogenesis
LigandGTP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-70263 Gluconeogenesis
SABIO-RKiQ9Z2V4
UniPathwayiUPA00138

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase, cytosolic [GTP] (EC:4.1.1.32By similarity)
Short name:
PEPCK-C
Gene namesi
Name:Pck1Imported
Synonyms:Pepck
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:97501 Pck1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001036281 – 622Phosphoenolpyruvate carboxykinase, cytosolic [GTP]Add BLAST622

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei19PhosphoserineCombined sources1
Modified residuei70N6-acetyllysine; by p300/EP300By similarity1
Modified residuei71N6-acetyllysine; by p300/EP300By similarity1
Modified residuei118PhosphoserineCombined sources1
Modified residuei286PhosphoserineBy similarity1

Post-translational modificationi

Lysine acetylation by p300/EP300 is increased on high glucose conditions and promotes ubiquitination by UBR5, acetylation is enhanced in the presence of BAG6. Deacetylated by SIRT2 (By similarity).By similarity
Ubiquitination by UBR5 leads to proteasomal degradation.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ9Z2V4
PaxDbiQ9Z2V4
PRIDEiQ9Z2V4

PTM databases

iPTMnetiQ9Z2V4
PhosphoSitePlusiQ9Z2V4
SwissPalmiQ9Z2V4

Expressioni

Inductioni

Regulated by glucocortinoids and insulin.1 Publication

Gene expression databases

BgeeiENSMUSG00000027513
CleanExiMM_PCK1
GenevisibleiQ9Z2V4 MM

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi202046, 1 interactor
IntActiQ9Z2V4, 1 interactor
STRINGi10090.ENSMUSP00000029017

Structurei

3D structure databases

ProteinModelPortaliQ9Z2V4
SMRiQ9Z2V4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni235 – 237Substrate bindingBy similarity3
Regioni403 – 405Substrate bindingBy similarity3

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3749 Eukaryota
COG1274 LUCA
GeneTreeiENSGT00390000001912
HOGENOMiHOG000191700
HOVERGENiHBG053651
InParanoidiQ9Z2V4
KOiK01596
OMAiCWLALTD
OrthoDBiEOG091G02YK
PhylomeDBiQ9Z2V4
TreeFamiTF314402

Family and domain databases

CDDicd00819 PEPCK_GTP, 1 hit
Gene3Di3.40.449.10, 2 hits
3.90.228.20, 2 hits
HAMAPiMF_00452 PEPCK_GTP, 1 hit
InterProiView protein in InterPro
IPR018091 PEP_carboxykin_GTP_CS
IPR013035 PEP_carboxykinase_C
IPR008209 PEP_carboxykinase_GTP
IPR035077 PEP_carboxykinase_GTP_C
IPR035078 PEP_carboxykinase_GTP_N
IPR008210 PEP_carboxykinase_N
PANTHERiPTHR11561 PTHR11561, 1 hit
PfamiView protein in Pfam
PF00821 PEPCK_C, 1 hit
PF17297 PEPCK_N, 1 hit
PIRSFiPIRSF001348 PEP_carboxykinase_GTP, 1 hit
SUPFAMiSSF68923 SSF68923, 1 hit
PROSITEiView protein in PROSITE
PS00505 PEPCK_GTP, 1 hit

Sequencei

Sequence statusi: Complete.

Q9Z2V4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPQLHNGLD FSAKVIQGSL DSLPQAVRKF VEGNAQLCQP EYIHICDGSE
60 70 80 90 100
EEYGQLLAHM QEEGVIRKLK KYDNCWLALT DPRDVARIES KTVIITQEQR
110 120 130 140 150
DTVPIPKTGL SQLGRWMSEE DFEKAFNARF PGCMKGRTMY VIPFSMGPLG
160 170 180 190 200
SPLAKIGIEL TDSPYVVASM RIMTRMGISV LEALGDGEFI KCLHSVGCPL
210 220 230 240 250
PLKKPLVNNW ACNPELTLIA HLPDRREIIS FGSGYGGNSL LGKKCFALRI
260 270 280 290 300
ASRLAKEEGW LAEHMLILGI TNPEGKKKYL AAAFPSACGK TNLAMMNPSL
310 320 330 340 350
PGWKVECVGD DIAWMKFDAQ GNLRAINPEN GFFGVAPGTS VKTNPNAIKT
360 370 380 390 400
IQKNTIFTNV AETSDGGVYW EGIDEPLAPG VTITSWKNKE WRPQDAEPCA
410 420 430 440 450
HPNSRFCTPA SQCPIIDPAW ESPEGVPIEG IIFGGRRPEG VPLVYEALSW
460 470 480 490 500
QHGVFVGAAM RSEATAAAEH KGKIIMHDPF AMRPFFGYNF GKYLAHWLSM
510 520 530 540 550
AHRPAAKLPK IFHVNWFRKD KDGKFLWPGF GENSRVLEWM FGRIEGEDSA
560 570 580 590 600
KLTPIGYIPK ENALNLKGLG GVNVEELFGI SKEFWEKEVE EIDRYLEDQV
610 620
NTDLPYEIER ELRALKQRIS QM
Length:622
Mass (Da):69,355
Last modified:May 1, 1999 - v1
Checksum:i9D94738B4A0EE196
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF009605 Genomic DNA Translation: AAD01427.1
AK028046 mRNA Translation: BAC25718.1
AK133496 mRNA Translation: BAE21687.1
AK149525 mRNA Translation: BAE28938.1
CCDSiCCDS17141.1
RefSeqiNP_035174.1, NM_011044.2
UniGeneiMm.266867

Genome annotation databases

EnsembliENSMUST00000029017; ENSMUSP00000029017; ENSMUSG00000027513
GeneIDi18534
KEGGimmu:18534
UCSCiuc008odm.1 mouse

Similar proteinsi

Entry informationi

Entry nameiPCKGC_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2V4
Secondary accession number(s): Q3UEH3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: May 1, 1999
Last modified: May 23, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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