Q9Z2V2 (CD40L_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CD40 ligand
Short name=CD40-L

Alternative name(s):
Tumor necrosis factor ligand superfamily member 5
CD_antigen=CD154

Cleaved into the following 2 chains:

1. CD40 ligand, membrane form
2. CD40 ligand, soluble form

Gene names

Name:	Cd40lg
Synonyms:	Cd40l, Tnfsf5

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Cytokine that binds to TNFRSF5. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching By similarity.
Subunit structure	Homotrimer By similarity.
Subcellular location	Cell membrane; Single-pass type II membrane protein By similarity. CD40 ligand, soluble form: Secreted By similarity.
Post-translational modification	The soluble form derives from the membrane form by proteolytic processing By similarity.
Sequence similarities	Belongs to the tumor necrosis factor family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane Secreted
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Cytokine
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	B cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB dendritic cell differentiation Inferred from mutant phenotype. Source: RGD inflammatory response Inferred from sequence or structural similarity. Source: UniProtKB platelet activation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of B cell proliferation Inferred from direct assay. Source: RGD positive regulation of MHC class II biosynthetic process Inferred from direct assay. Source: RGD positive regulation of immune response to tumor cell Inferred from direct assay. Source: RGD positive regulation of interferon-gamma production Inferred from direct assay. Source: RGD positive regulation of interleukin-10 production Inferred from direct assay. Source: RGD positive regulation of interleukin-12 production Inferred from direct assay. Source: RGD
Cellular component	extracellular space Inferred from direct assay. Source: RGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	CD40 receptor binding Inferred from sequence or structural similarity. Source: UniProtKB cytokine activity Inferred from electronic annotation. Source: UniProtKB-KW tumor necrosis factor receptor binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 260	260	CD40 ligand, membrane form		PRO_0000034494
	Chain	112 – 260	149	CD40 ligand, soluble form By similarity		PRO_0000034495
Regions
	Topological domain	1 – 22	22	Cytoplasmic Potential
	Transmembrane	23 – 43	21	Helical; Signal-anchor for type II membrane protein; Potential
	Topological domain	44 – 260	217	Extracellular Potential
Sites
	Site	111 – 112	2	Cleavage By similarity
Amino acid modifications
	Modified residue	4	1	Phosphothreonine By similarity
	Modified residue	6	1	Phosphoserine By similarity
	Modified residue	149	1	Phosphoserine By similarity
	Glycosylation	239	1	N-linked (GlcNAc...) Potential
	Disulfide bond	177 ↔ 217		Potential
Experimental info
	Sequence conflict	41	1	P → L in AAD09323. Ref.1
	Sequence conflict	114	1	R → K in AAD09323. Ref.1
	Sequence conflict	255	1	I → F in AAD09323. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Q9Z2V2 [UniParc]. Last modified June 6, 2002. Version 2. Checksum: B3D3757DE60DB73A	FASTA	260	29,260
	10 20 30 40 50 60 MIETYSQPSP RSVATGLPAS MKIFMYLLTV FLITQMIGSV PFAVYLHRRL DKVEEEASLH 70 80 90 100 110 120 EDFVFVKKLK RCNKGEGSLS LLNCEEMRRQ FEDLVKDISL NKEEKKEKSF EMQRGDEDPQ 130 140 150 160 170 180 IAAHVVSEAN SNAASVLQWA KKGYYTMKSN LVVLENGRQL TVKREGLYYV YTQVTFCSNR 190 200 210 220 230 240 EPLSQRPFIV SLWLKPSSGS ERILLRAANT HSSSKLCEQQ SIHLGGVFEL QAGASVFVNV 250 260 TEASQVIHGI GFSSIGLLKL

References

[1]	"Nucleotide sequence of the rat CD40 ligand." Hallett K.M., Oaks M.K. DNA Seq. 10:405-406(2000) [PubMed: 10826698] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Splenocyte.
[2]	"Cloning and sequencing of rat CD40 ligand." Daniel K.C., Foss Y., Moussavi A., Macary P., Kemeny D.M., Farzaneh F., Gaken J.A. Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: PVG. Tissue: Spleen.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF116582 mRNA. Translation: AAD22460.1. AF013985 mRNA. Translation: AAD09323.1.
IPI	IPI00210301.
RefSeq	NP_445805.1. NM_053353.1.
UniGene	Rn.44218.
3D structure databases
ProteinModelPortal	Q9Z2V2.
SMR	Q9Z2V2. Positions 115-260.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9Z2V2.
Proteomic databases
PRIDE	Q9Z2V2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	84349.
KEGG	rno:84349.
Organism-specific databases
CTD	959.
RGD	708418. Cd40lg.
Phylogenomic databases
GeneTree	ENSGT00510000048489.
HOVERGEN	HBG079629.
InParanoid	Q9Z2V2.
OrthoDB	EOG4G4GRV.
Gene expression databases
ArrayExpress	Q9Z2V2.
Genevestigator	Q9Z2V2.
GermOnline	ENSRNOG00000000871. Rattus norvegicus.
Family and domain databases
InterPro	IPR006052. TNF. IPR021184. TNF_CS. IPR003263. TNF_ligand_5. IPR008983. Tumour_necrosis_fac-like. [Graphical view]
Gene3D	G3DSA:2.60.120.40. Tumour_necrosis_fac-like. 1 hit.
KO	K03161.
Pfam	PF00229. TNF. 1 hit. [Graphical view]
PIRSF	PIRSF016527. TNF_5. 1 hit.
PRINTS	PR01702. CD40LIGAND.
SMART	SM00207. TNF. 1 hit. [Graphical view]
SUPFAM	SSF49842. TNF_like. 1 hit.
PROSITE	PS00251. TNF_1. 1 hit. PS50049. TNF_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	616651.

Entry information

Entry name

CD40L_RAT

Accession

Primary (citable) accession number: Q9Z2V2
Secondary accession number(s): Q9R254

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2002
Last sequence update:	June 6, 2002
Last modified:	January 25, 2012
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program