Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

CD40 ligand

Gene

Cd40lg

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Cytokine that binds to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching.By similarity
Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.By similarity

GO - Molecular functioni

GO - Biological processi

  • activation of JUN kinase activity Source: UniProtKB
  • B cell proliferation Source: UniProtKB
  • cellular response to tumor necrosis factor Source: RGD
  • dendritic cell differentiation Source: RGD
  • immune response Source: InterPro
  • inflammatory response Source: UniProtKB
  • platelet activation Source: UniProtKB
  • positive regulation of B cell proliferation Source: RGD
  • positive regulation of dendritic cell differentiation Source: RGD
  • positive regulation of gene expression Source: RGD
  • positive regulation of immune response to tumor cell Source: RGD
  • positive regulation of interferon-gamma production Source: RGD
  • positive regulation of interleukin-10 production Source: RGD
  • positive regulation of interleukin-12 production Source: RGD
  • positive regulation of interleukin-4 production Source: UniProtKB
  • positive regulation of MHC class II biosynthetic process Source: RGD
  • positive regulation of NF-kappaB transcription factor activity Source: UniProtKB
  • positive regulation of T cell proliferation Source: UniProtKB
  • response to cobalamin Source: RGD
  • response to peptide Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Names & Taxonomyi

Protein namesi
Recommended name:
CD40 ligand
Short name:
CD40-L
Alternative name(s):
Tumor necrosis factor ligand superfamily member 5
CD_antigen: CD154
Cleaved into the following 2 chains:
Gene namesi
Name:Cd40lg
Synonyms:Cd40l, Tnfsf5
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi708418. Cd40lg.

Subcellular locationi

  • Cell membrane By similarity; Single-pass type II membrane protein By similarity
  • Cell surface By similarity
CD40 ligand, soluble form :
  • Secreted By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 22CytoplasmicSequence analysisAdd BLAST22
Transmembranei23 – 43Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini44 – 260ExtracellularSequence analysisAdd BLAST217

GO - Cellular componenti

  • cell body Source: RGD
  • cell projection Source: RGD
  • cell surface Source: RGD
  • extracellular space Source: RGD
  • integral component of membrane Source: UniProtKB-KW
  • intracellular Source: GOC
  • plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000344941 – 260CD40 ligand, membrane formAdd BLAST260
ChainiPRO_0000034495112 – 260CD40 ligand, soluble formBy similarityAdd BLAST149

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi177 ↔ 217Sequence analysis
Glycosylationi239N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

The soluble form derives from the membrane form by proteolytic processing.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei111 – 112CleavageBy similarity2

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiQ9Z2V2.
PRIDEiQ9Z2V2.

PTM databases

iPTMnetiQ9Z2V2.
PhosphoSitePlusiQ9Z2V2.

Interactioni

Subunit structurei

Homotrimer. Interacts with CD28.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000001162.

Structurei

3D structure databases

ProteinModelPortaliQ9Z2V2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tumor necrosis factor family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IVYF. Eukaryota.
ENOG4111TET. LUCA.
HOGENOMiHOG000111291.
HOVERGENiHBG079629.
InParanoidiQ9Z2V2.
KOiK03161.
PhylomeDBiQ9Z2V2.

Family and domain databases

Gene3Di2.60.120.40. 1 hit.
InterProiIPR003263. CD40L.
IPR021184. TNF_CS.
IPR006052. TNF_dom.
IPR008983. Tumour_necrosis_fac-like_dom.
[Graphical view]
PfamiPF00229. TNF. 1 hit.
[Graphical view]
PIRSFiPIRSF016527. TNF_5. 1 hit.
PRINTSiPR01702. CD40LIGAND.
SMARTiSM00207. TNF. 1 hit.
[Graphical view]
SUPFAMiSSF49842. SSF49842. 1 hit.
PROSITEiPS00251. TNF_1. 1 hit.
PS50049. TNF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z2V2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIETYSQPSP RSVATGLPAS MKIFMYLLTV FLITQMIGSV PFAVYLHRRL
60 70 80 90 100
DKVEEEASLH EDFVFVKKLK RCNKGEGSLS LLNCEEMRRQ FEDLVKDISL
110 120 130 140 150
NKEEKKEKSF EMQRGDEDPQ IAAHVVSEAN SNAASVLQWA KKGYYTMKSN
160 170 180 190 200
LVVLENGRQL TVKREGLYYV YTQVTFCSNR EPLSQRPFIV SLWLKPSSGS
210 220 230 240 250
ERILLRAANT HSSSKLCEQQ SIHLGGVFEL QAGASVFVNV TEASQVIHGI
260
GFSSIGLLKL
Length:260
Mass (Da):29,260
Last modified:June 6, 2002 - v2
Checksum:iB3D3757DE60DB73A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti41P → L in AAD09323 (PubMed:10826698).Curated1
Sequence conflicti114R → K in AAD09323 (PubMed:10826698).Curated1
Sequence conflicti255I → F in AAD09323 (PubMed:10826698).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF116582 mRNA. Translation: AAD22460.1.
AF013985 mRNA. Translation: AAD09323.1.
RefSeqiNP_445805.1. NM_053353.1.
UniGeneiRn.44218.

Genome annotation databases

GeneIDi84349.
KEGGirno:84349.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF116582 mRNA. Translation: AAD22460.1.
AF013985 mRNA. Translation: AAD09323.1.
RefSeqiNP_445805.1. NM_053353.1.
UniGeneiRn.44218.

3D structure databases

ProteinModelPortaliQ9Z2V2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000001162.

PTM databases

iPTMnetiQ9Z2V2.
PhosphoSitePlusiQ9Z2V2.

Proteomic databases

PaxDbiQ9Z2V2.
PRIDEiQ9Z2V2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi84349.
KEGGirno:84349.

Organism-specific databases

CTDi959.
RGDi708418. Cd40lg.

Phylogenomic databases

eggNOGiENOG410IVYF. Eukaryota.
ENOG4111TET. LUCA.
HOGENOMiHOG000111291.
HOVERGENiHBG079629.
InParanoidiQ9Z2V2.
KOiK03161.
PhylomeDBiQ9Z2V2.

Miscellaneous databases

PROiQ9Z2V2.

Family and domain databases

Gene3Di2.60.120.40. 1 hit.
InterProiIPR003263. CD40L.
IPR021184. TNF_CS.
IPR006052. TNF_dom.
IPR008983. Tumour_necrosis_fac-like_dom.
[Graphical view]
PfamiPF00229. TNF. 1 hit.
[Graphical view]
PIRSFiPIRSF016527. TNF_5. 1 hit.
PRINTSiPR01702. CD40LIGAND.
SMARTiSM00207. TNF. 1 hit.
[Graphical view]
SUPFAMiSSF49842. SSF49842. 1 hit.
PROSITEiPS00251. TNF_1. 1 hit.
PS50049. TNF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCD40L_RAT
AccessioniPrimary (citable) accession number: Q9Z2V2
Secondary accession number(s): Q9R254
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: June 6, 2002
Last modified: November 2, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.