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Protein

ETS-related transcription factor Elf-4

Gene

Elf4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Transcriptional activator that binds to DNA sequences containing the consensus 5'-WGGA-3'. Transactivates promoters of the hematopoietic growth factor genes CSF2, IL3, IL8, and of LYZ. Acts synergistically with RUNX1 to transactivate the IL3 promoter (By similarity). Also transactivates the PRF1 promoter in natural killer (NK) cells. Plays a role in the development and function of NK and NK T-cells and in innate immunity. Controls the proliferation and homing of CD8+ T-cells via the Kruppel-like factors KLF4 and KLF2. Controls cell senescence in a p53-dependent manner. Can also promote cellular transformation through inhibition of the p16 pathway.By similarity3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi208 – 29083ETSPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • cell differentiation Source: GO_Central
  • natural killer cell proliferation Source: UniProtKB
  • NK T cell proliferation Source: UniProtKB
  • positive regulation of transcription, DNA-templated Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
ETS-related transcription factor Elf-4
Alternative name(s):
E74-like factor 4
Myeloid Elf-1-like factor
Gene namesi
Name:Elf4Imported
Synonyms:MefImported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1928377. Elf4.

Subcellular locationi

  • NucleusPML body By similarity

  • Note: Accumulation into PML nuclear bodies is mediated by PML.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 655655ETS-related transcription factor Elf-4PRO_0000204090Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei640 – 6401PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9Z2U4.
PRIDEiQ9Z2U4.

PTM databases

PhosphoSiteiQ9Z2U4.

Expressioni

Gene expression databases

BgeeiQ9Z2U4.
ExpressionAtlasiQ9Z2U4. baseline and differential.
GenevisibleiQ9Z2U4. MM.

Interactioni

Subunit structurei

Interacts with RUNX1 (via the Runt domain); the interaction transactivates the IL3 promoter. Interacts (via its C-terminus) with PML; the interaction translocates ELF4 to PML nuclear bodies and enhances transactivation of LYZ (By similarity).By similarity

Protein-protein interaction databases

BioGridi208021. 1 interaction.
STRINGi10090.ENSMUSP00000033429.

Structurei

3D structure databases

ProteinModelPortaliQ9Z2U4.
SMRiQ9Z2U4. Positions 208-316.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni86 – 205120RUNX1-bindingBy similarityAdd
BLAST

Sequence similaritiesi

Belongs to the ETS family.Sequence Analysis
Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG291231.
GeneTreeiENSGT00760000118996.
HOGENOMiHOG000049253.
HOVERGENiHBG007183.
InParanoidiQ9Z2U4.
KOiK09428.
OMAiSFQENQV.
OrthoDBiEOG73RBD1.
TreeFamiTF318679.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000418. Ets_dom.
IPR022084. TF_Elf_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF12310. Elf-1_N. 1 hit.
PF00178. Ets. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z2U4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIALQPSDL VFEFASNGMD DIHQLEDPSV FPAVIVEQVP YPELVHLCSG
60 70 80 90 100
LDLDEVHNGI IRDRTLCMTQ DQILEGSILL TDDDVSTSNN VSSTEVLFNV
110 120 130 140 150
ATPSDVLDEK QIFSSPEVLS DSNSVQAINL PNFLLSTPEP DDLKKTSDAG
160 170 180 190 200
DQKEHSEEEK VSREENLRKM GKARKRNRKT KNNRSTSPVT DPSMPIRKKS
210 220 230 240 250
KDGKGSTIYL WEFLLALLQD RNTCPKYIKW TQREKGIFKL VDSKAVSKLW
260 270 280 290 300
GKQKNKPDMN YETMGRALRY YYQRGILAKV EGQRLVYQFK EMPKDLVVID
310 320 330 340 350
DEEESPETPE DSSQASTSST PSTSTIRRAS SRVGTRASPE DKDNPPWEKP
360 370 380 390 400
KVQHTGLQPS ASLELGLSVD EEVPTTSTML ASPLQSQARL TKTVSSSPAP
410 420 430 440 450
SNIHLGVAPV GPGSTVTLQT IPLTTVLTNG PPASTTAPTQ LVLQSVPQVS
460 470 480 490 500
TFKDTFTLQT SFPLNTNLQE NQVATQGAPL ILSGLPQLLA GANPQSNPAP
510 520 530 540 550
SQVIGAGSAG PSSQPPGTVI AAFIRTSSGT SVPVVKEGPL RSSSYVQGVV
560 570 580 590 600
TGAPVEGLLV PEETLRELLR DQGHLQPLPS QVLSRGSHNL SLVGNQTLSP
610 620 630 640 650
PSHPTVGLTP VAELELSSGS GPLFVTEPSV TRSPTQAPFS PFNPTSLIKM

EPQDI
Length:655
Mass (Da):70,800
Last modified:July 27, 2011 - v2
Checksum:i44CAFA1D3D77153C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti132 – 1321N → K in AAD01601 (Ref. 1) Curated
Sequence conflicti146 – 1461T → A in AAD01601 (Ref. 1) Curated
Sequence conflicti344 – 3441N → S in AAD01601 (Ref. 1) Curated
Sequence conflicti448 – 4481Q → A in AAD01601 (Ref. 1) Curated
Sequence conflicti496 – 4961S → T in AAD01601 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF016714 mRNA. Translation: AAD01601.1.
AL844594 Genomic DNA. Translation: CAM18942.1.
CCDSiCCDS30108.1.
RefSeqiNP_062654.2. NM_019680.2.
XP_006541589.1. XM_006541526.1.
XP_006541590.1. XM_006541527.1.
UniGeneiMm.36808.

Genome annotation databases

EnsembliENSMUST00000033429; ENSMUSP00000033429; ENSMUSG00000031103.
ENSMUST00000114958; ENSMUSP00000110608; ENSMUSG00000031103.
GeneIDi56501.
KEGGimmu:56501.
UCSCiuc009tce.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF016714 mRNA. Translation: AAD01601.1.
AL844594 Genomic DNA. Translation: CAM18942.1.
CCDSiCCDS30108.1.
RefSeqiNP_062654.2. NM_019680.2.
XP_006541589.1. XM_006541526.1.
XP_006541590.1. XM_006541527.1.
UniGeneiMm.36808.

3D structure databases

ProteinModelPortaliQ9Z2U4.
SMRiQ9Z2U4. Positions 208-316.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi208021. 1 interaction.
STRINGi10090.ENSMUSP00000033429.

PTM databases

PhosphoSiteiQ9Z2U4.

Proteomic databases

MaxQBiQ9Z2U4.
PRIDEiQ9Z2U4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033429; ENSMUSP00000033429; ENSMUSG00000031103.
ENSMUST00000114958; ENSMUSP00000110608; ENSMUSG00000031103.
GeneIDi56501.
KEGGimmu:56501.
UCSCiuc009tce.2. mouse.

Organism-specific databases

CTDi2000.
MGIiMGI:1928377. Elf4.

Phylogenomic databases

eggNOGiNOG291231.
GeneTreeiENSGT00760000118996.
HOGENOMiHOG000049253.
HOVERGENiHBG007183.
InParanoidiQ9Z2U4.
KOiK09428.
OMAiSFQENQV.
OrthoDBiEOG73RBD1.
TreeFamiTF318679.

Miscellaneous databases

NextBioi312794.
PROiQ9Z2U4.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Z2U4.
ExpressionAtlasiQ9Z2U4. baseline and differential.
GenevisibleiQ9Z2U4. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000418. Ets_dom.
IPR022084. TF_Elf_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF12310. Elf-1_N. 1 hit.
PF00178. Ets. 1 hit.
[Graphical view]
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 1 hit.
PS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Murine MEF (myeloid elf-1-like factor) cDNA clone."
    Miyazaki Y., De Blasio T., Nimer S.D.
    Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "The ETS protein MEF plays a critical role in perforin gene expression and the development of natural killer and NK-T cells."
    Lacorazza H.D., Miyazaki Y., Di Cristofano A., Deblasio A., Hedvat C., Zhang J., Cordon-Cardo C., Mao S., Pandolfi P.P., Nimer S.D.
    Immunity 17:437-449(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "ELF4/MEF activates MDM2 expression and blocks oncogene-induced p16 activation to promote transformation."
    Sashida G., Liu Y., Elf S., Miyata Y., Ohyashiki K., Izumi M., Menendez S., Nimer S.D.
    Mol. Cell. Biol. 29:3687-3699(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  5. "Transcription factor ELF4 controls the proliferation and homing of CD8+ T cells via the Kruppel-like factors KLF4 and KLF2."
    Yamada T., Park C.S., Mamonkin M., Lacorazza H.D.
    Nat. Immunol. 10:618-626(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiELF4_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2U4
Secondary accession number(s): B1AY67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: July 27, 2011
Last modified: July 22, 2015
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.