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Q9Z2U4 (ELF4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ETS-related transcription factor Elf-4
Alternative name(s):
E74-like factor 4
Myeloid Elf-1-like factor
Gene names
Name:Elf4
Synonyms:Mef
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length655 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcriptional activator that binds to DNA sequences containing the consensus 5'-WGGA-3'. Transactivates promoters of the hematopoietic growth factor genes CSF2, IL3, IL8, and of LYZ. Acts synergistically with RUNX1 to transactivate the IL3 promoter By similarity. Also transactivates the PRF1 promoter in natural killer (NK) cells. Plays a role in the development and function of NK and NK T-cells and in innate immunity. Controls the proliferation and homing of CD8+ T-cells via the Kruppel-like factors KLF4 and KLF2. Controls cell senescence in a p53-dependent manner. Can also promote cellular transformation through inhibition of the p16 pathway. Ref.3 Ref.4 Ref.5 UniProtKB Q99607

Subunit structure

Interacts with RUNX1 (via the Runt domain); the interaction transactivates the IL3 promoter. Interacts (via its C-terminus) with PML; the interaction translocates ELF4 to PML nuclear bodies and enhances transactivation of LYZ By similarity.

Subcellular location

NucleusPML body By similarity. Note: Accumulation into PML nuclear bodies is mediated by PML By similarity.

Sequence similarities

Belongs to the ETS family.

Contains 1 ETS DNA-binding domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 655655ETS-related transcription factor Elf-4
PRO_0000204090

Regions

DNA binding208 – 29083ETS
Region86 – 205120RUNX1-binding By similarity UniProtKB Q99607

Amino acid modifications

Modified residue6401Phosphoserine By similarity

Experimental info

Sequence conflict1321N → K in AAD01601. Ref.1
Sequence conflict1461T → A in AAD01601. Ref.1
Sequence conflict3441N → S in AAD01601. Ref.1
Sequence conflict4481Q → A in AAD01601. Ref.1
Sequence conflict4961S → T in AAD01601. Ref.1

Sequences

Sequence LengthMass (Da)Tools
Q9Z2U4 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 44CAFA1D3D77153C

FASTA65570,800
        10         20         30         40         50         60 
MAIALQPSDL VFEFASNGMD DIHQLEDPSV FPAVIVEQVP YPELVHLCSG LDLDEVHNGI 

        70         80         90        100        110        120 
IRDRTLCMTQ DQILEGSILL TDDDVSTSNN VSSTEVLFNV ATPSDVLDEK QIFSSPEVLS 

       130        140        150        160        170        180 
DSNSVQAINL PNFLLSTPEP DDLKKTSDAG DQKEHSEEEK VSREENLRKM GKARKRNRKT 

       190        200        210        220        230        240 
KNNRSTSPVT DPSMPIRKKS KDGKGSTIYL WEFLLALLQD RNTCPKYIKW TQREKGIFKL 

       250        260        270        280        290        300 
VDSKAVSKLW GKQKNKPDMN YETMGRALRY YYQRGILAKV EGQRLVYQFK EMPKDLVVID 

       310        320        330        340        350        360 
DEEESPETPE DSSQASTSST PSTSTIRRAS SRVGTRASPE DKDNPPWEKP KVQHTGLQPS 

       370        380        390        400        410        420 
ASLELGLSVD EEVPTTSTML ASPLQSQARL TKTVSSSPAP SNIHLGVAPV GPGSTVTLQT 

       430        440        450        460        470        480 
IPLTTVLTNG PPASTTAPTQ LVLQSVPQVS TFKDTFTLQT SFPLNTNLQE NQVATQGAPL 

       490        500        510        520        530        540 
ILSGLPQLLA GANPQSNPAP SQVIGAGSAG PSSQPPGTVI AAFIRTSSGT SVPVVKEGPL 

       550        560        570        580        590        600 
RSSSYVQGVV TGAPVEGLLV PEETLRELLR DQGHLQPLPS QVLSRGSHNL SLVGNQTLSP 

       610        620        630        640        650 
PSHPTVGLTP VAELELSSGS GPLFVTEPSV TRSPTQAPFS PFNPTSLIKM EPQDI 

« Hide

References

« Hide 'large scale' references
[1]"Murine MEF (myeloid elf-1-like factor) cDNA clone."
Miyazaki Y., De Blasio T., Nimer S.D.
Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"The ETS protein MEF plays a critical role in perforin gene expression and the development of natural killer and NK-T cells."
Lacorazza H.D., Miyazaki Y., Di Cristofano A., Deblasio A., Hedvat C., Zhang J., Cordon-Cardo C., Mao S., Pandolfi P.P., Nimer S.D.
Immunity 17:437-449(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"ELF4/MEF activates MDM2 expression and blocks oncogene-induced p16 activation to promote transformation."
Sashida G., Liu Y., Elf S., Miyata Y., Ohyashiki K., Izumi M., Menendez S., Nimer S.D.
Mol. Cell. Biol. 29:3687-3699(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[5]"Transcription factor ELF4 controls the proliferation and homing of CD8+ T cells via the Kruppel-like factors KLF4 and KLF2."
Yamada T., Park C.S., Mamonkin M., Lacorazza H.D.
Nat. Immunol. 10:618-626(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF016714 mRNA. Translation: AAD01601.1.
AL844594 Genomic DNA. Translation: CAM18942.1.
CCDSCCDS30108.1.
RefSeqNP_062654.2. NM_019680.2.
XP_006541589.1. XM_006541526.1.
XP_006541590.1. XM_006541527.1.
UniGeneMm.36808.

3D structure databases

ProteinModelPortalQ9Z2U4.
SMRQ9Z2U4. Positions 208-289.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid208021. 1 interaction.

PTM databases

PhosphoSiteQ9Z2U4.

Proteomic databases

PRIDEQ9Z2U4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000033429; ENSMUSP00000033429; ENSMUSG00000031103.
ENSMUST00000114958; ENSMUSP00000110608; ENSMUSG00000031103.
GeneID56501.
KEGGmmu:56501.
UCSCuc009tce.2. mouse.

Organism-specific databases

CTD2000.
MGIMGI:1928377. Elf4.

Phylogenomic databases

eggNOGNOG291231.
GeneTreeENSGT00750000117299.
HOGENOMHOG000049253.
HOVERGENHBG007183.
InParanoidB1AY67.
KOK09428.
OMAYLFPVSE.
OrthoDBEOG73RBD1.
TreeFamTF318679.

Gene expression databases

BgeeQ9Z2U4.
GenevestigatorQ9Z2U4.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
InterProIPR000418. Ets_dom.
IPR022084. TF_Elf_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF12310. Elf-1_N. 1 hit.
PF00178. Ets. 1 hit.
[Graphical view]
PRINTSPR00454. ETSDOMAIN.
SMARTSM00413. ETS. 1 hit.
[Graphical view]
PROSITEPS00018. EF_HAND_1. 1 hit.
PS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio312794.
PROQ9Z2U4.
SOURCESearch...

Entry information

Entry nameELF4_MOUSE
AccessionPrimary (citable) accession number: Q9Z2U4
Secondary accession number(s): B1AY67
Entry history
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: July 27, 2011
Last modified: July 9, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot