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Protein

Flotillin-2

Gene

Flot2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May play a role in axon growth and regeneration. May be involved in epidermal cell adhesion and epidermal structure and function.1 Publication

GO - Molecular functioni

  • ionotropic glutamate receptor binding Source: RGD
  • protease binding Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiR-RNO-8849932. SALM protein interactions at the synapse.

Names & Taxonomyi

Protein namesi
Recommended name:
Flotillin-2
Alternative name(s):
Reggie-1
Short name:
REG-1
Gene namesi
Name:Flot2
Synonyms:Reg1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi70993. Flot2.

Subcellular locationi

  • Membrane 1 Publication
  • Endosome By similarity
  • Membrane By similarity; Lipid-anchor By similarity

  • Note: In neuronal cells, associated with GPI-anchored cell-adhesion molecules.

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 428427Flotillin-2PRO_0000094051Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity
Lipidationi4 – 41S-palmitoyl cysteine; by ZDHHC5By similarity
Lipidationi19 – 191S-palmitoyl cysteineBy similarity
Lipidationi20 – 201S-palmitoyl cysteine; by ZDHHC5By similarity
Modified residuei405 – 4051PhosphoserineBy similarity

Post-translational modificationi

ZDHHC5-catalyzed palmitoylation may be required for the formation of higher-order complexes and for neurite outgrowth in cultured neural stem cells.By similarity

Keywords - PTMi

Lipoprotein, Myristate, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ9Z2S9.
PRIDEiQ9Z2S9.

PTM databases

iPTMnetiQ9Z2S9.
PhosphoSiteiQ9Z2S9.
SwissPalmiQ9Z2S9.

Expressioni

Tissue specificityi

Brain, retina and skin.1 Publication

Developmental stagei

Expressed during embryogenesis, postnatal stages and in adult.1 Publication

Inductioni

By optic nerve injury.1 Publication

Gene expression databases

ExpressionAtlasiQ9Z2S9. baseline and differential.
GenevisibleiQ9Z2S9. RN.

Interactioni

Subunit structurei

Heterooligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2. Interacts with ECM29 (By similarity).By similarity

GO - Molecular functioni

  • ionotropic glutamate receptor binding Source: RGD
  • protease binding Source: UniProtKB

Protein-protein interaction databases

IntActiQ9Z2S9. 3 interactions.
MINTiMINT-4622185.
STRINGi10116.ENSRNOP00000014118.

Structurei

3D structure databases

ProteinModelPortaliQ9Z2S9.
SMRiQ9Z2S9. Positions 40-173.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2668. Eukaryota.
COG2268. LUCA.
GeneTreeiENSGT00560000077232.
HOGENOMiHOG000240804.
HOVERGENiHBG051628.
InParanoidiQ9Z2S9.
KOiK07192.
OMAiCCGSDTK.
OrthoDBiEOG7N0C4V.
PhylomeDBiQ9Z2S9.

Family and domain databases

InterProiIPR001107. Band_7.
IPR031905. Flotillin_C.
IPR027705. Flotillin_fam.
[Graphical view]
PANTHERiPTHR13806. PTHR13806. 1 hit.
PfamiPF01145. Band_7. 1 hit.
PF15975. Flot. 1 hit.
[Graphical view]
SMARTiSM00244. PHB. 1 hit.
[Graphical view]
SUPFAMiSSF117892. SSF117892. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Z2S9-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGNCHTVGPN EALVVSGGCC GSDYKQYVFG GWAWAWWCIS DTQRISLEIM
60 70 80 90 100
TLQPRCEDVE TAEGVALTVT GVAQVKIMTE KELLAVACEQ FLGKNVQDIK
110 120 130 140 150
NVVLQTLEGH LRSILGTLTV EQIYQDRDQF AKLVREVAAP DVGRMGIEIL
160 170 180 190 200
SFTIKDVYDK VDYLSSLGKT QTAVVQRDAD IGVAEAERDA GIREAECKKE
210 220 230 240 250
MLDVKFMADT KIADSKRAFE LQKSAFSEEV NIKTAEAQLA YELQGAREQQ
260 270 280 290 300
KIRQEEIEIE VVQRKKQIAV EAQEILRTDK ELIATVRRPA EAEAHRIQQI
310 320 330 340 350
AEGEKVKQVL LAQAEAEKIR KIGEAEAAVI EAMGKAEAER MKLKAEAYQK
360 370 380 390 400
YGDAAKMALV LEALPQIAAK ISAPLTKVDE IVVLSGDNSK VTSEVNRLLA
410 420
ELPASVHALT GVDLSKIPLI KNATGAQV
Length:428
Mass (Da):47,038
Last modified:May 1, 1999 - v1
Checksum:iE482A0E2071D3CA7
GO
Isoform 2 (identifier: Q9Z2S9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     45-68: ISLEIMTLQPRCEDVETAEGVALT → LSLEVMTILCRCENIETSEGVPLF

Show »
Length:428
Mass (Da):47,116
Checksum:i57F051D4FCE679B8
GO
Isoform 4 (identifier: Q9Z2S9-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: MGNCHTVGPNEALVVSGGC → MTILCRCENIETSEGVPLF
     20-68: Missing.

Show »
Length:379
Mass (Da):41,751
Checksum:i9C7276300C6AD176
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti427 – 4271Q → K in AAD00120 (Ref. 2) Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 1919MGNCH…VSGGC → MTILCRCENIETSEGVPLF in isoform 4. 1 PublicationVSP_000503Add
BLAST
Alternative sequencei20 – 6849Missing in isoform 4. 1 PublicationVSP_000504Add
BLAST
Alternative sequencei45 – 6824ISLEI…GVALT → LSLEVMTILCRCENIETSEG VPLF in isoform 2. 1 PublicationVSP_000505Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF023302 mRNA. Translation: AAC98727.1.
AF023303 mRNA. Translation: AAC98728.1.
AF023304 mRNA. Translation: AAC98729.1.
U64999 mRNA. Translation: AAD00120.1.
RefSeqiNP_001257729.1. NM_001270800.1. [Q9Z2S9-1]
NP_114018.1. NM_031830.2. [Q9Z2S9-2]
UniGeneiRn.2105.

Genome annotation databases

EnsembliENSRNOT00000014104; ENSRNOP00000014104; ENSRNOG00000009681. [Q9Z2S9-1]
ENSRNOT00000014118; ENSRNOP00000014118; ENSRNOG00000009681. [Q9Z2S9-2]
GeneIDi83764.
KEGGirno:83764.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF023302 mRNA. Translation: AAC98727.1.
AF023303 mRNA. Translation: AAC98728.1.
AF023304 mRNA. Translation: AAC98729.1.
U64999 mRNA. Translation: AAD00120.1.
RefSeqiNP_001257729.1. NM_001270800.1. [Q9Z2S9-1]
NP_114018.1. NM_031830.2. [Q9Z2S9-2]
UniGeneiRn.2105.

3D structure databases

ProteinModelPortaliQ9Z2S9.
SMRiQ9Z2S9. Positions 40-173.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9Z2S9. 3 interactions.
MINTiMINT-4622185.
STRINGi10116.ENSRNOP00000014118.

PTM databases

iPTMnetiQ9Z2S9.
PhosphoSiteiQ9Z2S9.
SwissPalmiQ9Z2S9.

Proteomic databases

PaxDbiQ9Z2S9.
PRIDEiQ9Z2S9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000014104; ENSRNOP00000014104; ENSRNOG00000009681. [Q9Z2S9-1]
ENSRNOT00000014118; ENSRNOP00000014118; ENSRNOG00000009681. [Q9Z2S9-2]
GeneIDi83764.
KEGGirno:83764.

Organism-specific databases

CTDi2319.
RGDi70993. Flot2.

Phylogenomic databases

eggNOGiKOG2668. Eukaryota.
COG2268. LUCA.
GeneTreeiENSGT00560000077232.
HOGENOMiHOG000240804.
HOVERGENiHBG051628.
InParanoidiQ9Z2S9.
KOiK07192.
OMAiCCGSDTK.
OrthoDBiEOG7N0C4V.
PhylomeDBiQ9Z2S9.

Enzyme and pathway databases

ReactomeiR-RNO-8849932. SALM protein interactions at the synapse.

Miscellaneous databases

PROiQ9Z2S9.

Gene expression databases

ExpressionAtlasiQ9Z2S9. baseline and differential.
GenevisibleiQ9Z2S9. RN.

Family and domain databases

InterProiIPR001107. Band_7.
IPR031905. Flotillin_C.
IPR027705. Flotillin_fam.
[Graphical view]
PANTHERiPTHR13806. PTHR13806. 1 hit.
PfamiPF01145. Band_7. 1 hit.
PF15975. Flot. 1 hit.
[Graphical view]
SMARTiSM00244. PHB. 1 hit.
[Graphical view]
SUPFAMiSSF117892. SSF117892. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Identification of reggie-1 and reggie-2 as plasmamembrane-associated proteins which cocluster with activated GPI-anchored cell adhesion molecules in non-caveolar micropatches in neurons."
    Lang D.M., Lommel S., Jung M., Ankerhold R., Petrausch B., Laessing U., Wiechers M.F., Plattner H., Stuermer C.A.O.
    J. Neurobiol. 37:502-523(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 4), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBUNIT, INDUCTION.
    Strain: Sprague-Dawley.
    Tissue: Brain and Skin.
  2. Lommel S.M., Schulte T., Stuermer C.A.O.
    Submitted (JUL-1996) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.

Entry informationi

Entry nameiFLOT2_RAT
AccessioniPrimary (citable) accession number: Q9Z2S9
Secondary accession number(s): Q9QX33, Q9Z2S8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: May 1, 1999
Last modified: June 8, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.