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Protein

TSC22 domain family protein 3

Gene

Tsc22d3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protects T-cells from IL2 deprivation-induced apoptosis through the inhibition of FOXO3A transcriptional activity that leads to the down-regulation of the pro-apoptotic factor BCL2L11. In macrophages, plays a role in the anti-inflammatory and immunosuppressive effects of glucocorticoids and IL10. In T-cells, inhibits anti-CD3-induced NFKB1 nuclear translocation. In vitro, suppresses AP1 and NFKB1 DNA-binding activities (By similarity). Isoform 1 and isoform 4 inhibit myogenic differentiation and mediate anti-myogenic effects of glucocorticoids by binding and regulating MYOD1 and HDAC1 transcriptional activity resulting in reduced expression of MYOG.By similarity1 Publication

GO - Molecular functioni

  • MRF binding Source: MGI
  • transcription factor activity, sequence-specific DNA binding Source: GO_Central

GO - Biological processi

  • negative regulation of activation-induced cell death of T cells Source: MGI
  • negative regulation of skeletal muscle tissue development Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • response to osmotic stress Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Names & Taxonomyi

Protein namesi
Recommended name:
TSC22 domain family protein 3
Alternative name(s):
Glucocorticoid-induced leucine zipper protein
TSC22-related-inducible leucine zipper 3
Short name:
Tilz3
Gene namesi
Name:Tsc22d3
Synonyms:Dsip1, Dsipi, Gilz
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1196284. Tsc22d3.

Subcellular locationi

Isoform 1 :
  • Cytoplasm
  • Nucleus

  • Note: Localization depends on differentiation status of myoblasts. In undifferentiated myoblasts, isoform 1 localizes to the cytoplasm, but in differentiating myoblasts, isoform 1 is localized to the nucleus.
Isoform 4 :
  • Cytoplasm
  • Nucleus

  • Note: Localization depends on differentiation status of myoblasts. In undifferentiated myoblasts, isoform 4 localizes to the cytoplasm, but in differentiating myoblasts, isoform 4 is localized to the nucleus.

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002193711 – 137TSC22 domain family protein 3Add BLAST137

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei102PhosphoserineBy similarity1
Modified residuei125PhosphothreonineCombined sources1
Modified residuei128PhosphothreonineCombined sources1
Isoform 3 (identifier: Q9Z2S7-3)
Modified residuei40PhosphoserineCombined sources1
Isoform 4 (identifier: Q9Z2S7-4)
Modified residuei73PhosphoserineCombined sources1

Post-translational modificationi

Isoform 3 is phosphorylated on Ser-30 and Ser-40.

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9Z2S7.
PeptideAtlasiQ9Z2S7.
PRIDEiQ9Z2S7.

2D gel databases

REPRODUCTION-2DPAGEIPI00265379.

PTM databases

iPTMnetiQ9Z2S7.
PhosphoSitePlusiQ9Z2S7.

Expressioni

Tissue specificityi

Constitutively expressed in lung, intestine, kidney and liver, most probably by resident cells from the macrophage lineage. Expression inversely correlates with T-cell activation, being higher in resting cells and lower in cells activated by TCR/CD3 triggering. Isoform 1 and isoform 4 are expressed in spleen and skeletal muscle (at protein level). Isoform 1 is expressed in thymus, lymph nodes, bone marrow, spleen, lung and skeletal muscle.3 Publications

Developmental stagei

Isoform 1 and isoform 4 are expressed in differentiating myoblasts at a time of myotube formation.1 Publication

Inductioni

By glucocorticoids in lymphoid cells and upon IL4, IL10, IL13 or glucocorticoid treatment in monocyte/macrophage cells. Transiently induced by IL2 deprivation in T-cells. Isoform 1 and isoform 4 expression is up-regulated by the synthetic glucocorticoid dexamethasone in differentiating myoblasts.4 Publications

Gene expression databases

BgeeiENSMUSG00000031431.
CleanExiMM_TSC22D3.
ExpressionAtlasiQ9Z2S7. baseline and differential.
GenevisibleiQ9Z2S7. MM.

Interactioni

Subunit structurei

Can form homodimers, however it is likely to function as a monomer. Interacts with AP1 and NFKB1 (By similarity). Isoform 1 and isoform 4 interact with MYOD1. Isoform 1 interacts with HDAC1; this interaction affects HDAC1 activity on MYOG promoter and thus inhibits MYOD1 transcriptional activity.By similarity2 Publications

GO - Molecular functioni

  • MRF binding Source: MGI

Protein-protein interaction databases

BioGridi199919. 5 interactors.
DIPiDIP-48844N.
STRINGi10090.ENSMUSP00000108620.

Structurei

3D structure databases

ProteinModelPortaliQ9Z2S7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 60AP1-bindingAdd BLAST60
Regioni76 – 97Leucine-zipperAdd BLAST22

Domaini

The leucine-zipper is involved in homodimerization.

Sequence similaritiesi

Belongs to the TSC-22/Dip/Bun family.Curated

Phylogenomic databases

eggNOGiENOG410IRR1. Eukaryota.
ENOG410YAFT. LUCA.
GeneTreeiENSGT00530000063062.
HOGENOMiHOG000015349.
HOVERGENiHBG075918.
InParanoidiQ9Z2S7.
OMAiSTEMFAK.
OrthoDBiEOG091G06PM.
PhylomeDBiQ9Z2S7.
TreeFamiTF329224.

Family and domain databases

InterProiIPR000580. TSC-22_Dip_Bun.
[Graphical view]
PANTHERiPTHR12348. PTHR12348. 1 hit.
PfamiPF01166. TSC22. 1 hit.
[Graphical view]
ProDomiPD007152. TSC-22_Dip_Bun. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS01289. TSC22. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Z2S7-1) [UniParc]FASTAAdd to basket
Also known as: Tilz3b

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNTEMYQTPM EVAVYQLHNF SISFFSSLLG GDVVSVKLDN SASGASVVAL
60 70 80 90 100
DNKIEQAMDL VKNHLMYAVR EEVEVLKEQI RELLEKNSQL ERENTLLKTL
110 120 130
ASPEQLEKFQ SRLSPEEPAP EAPETPETPE APGGSAV
Length:137
Mass (Da):15,177
Last modified:February 1, 2005 - v2
Checksum:iA11D7B69037F111E
GO
Isoform 2 (identifier: Q9Z2S7-2) [UniParc]FASTAAdd to basket
Also known as: Tilz3a

The sequence of this isoform differs from the canonical sequence as follows:
     1-57: Missing.

Show »
Length:80
Mass (Da):9,040
Checksum:i2174BA0EC92DDCFC
GO
Isoform 3 (identifier: Q9Z2S7-3) [UniParc]FASTAAdd to basket
Also known as: Tilz3c

The sequence of this isoform differs from the canonical sequence as follows:
     1-40: MNTEMYQTPM...GDVVSVKLDN → MAQPKTECRS...TMLSILLFFH

Show »
Length:201
Mass (Da):22,565
Checksum:iB12F0480154A20DE
GO
Isoform 4 (identifier: Q9Z2S7-4) [UniParc]FASTAAdd to basket
Also known as: Long Gilz, L-Gilz

The sequence of this isoform differs from the canonical sequence as follows:
     1-40: MNTEMYQTPM...GDVVSVKLDN → MESQKASSAG...TMLSILLFFH

Show »
Length:234
Mass (Da):25,778
Checksum:iC7CEDB07D24D4185
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti22I → T in AAD01789 (PubMed:9430225).Curated1
Sequence conflicti33V → L in AAG41221 (Ref. 3) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0126901 – 57Missing in isoform 2. 2 PublicationsAdd BLAST57
Alternative sequenceiVSP_0126911 – 40MNTEM…VKLDN → MAQPKTECRSPVGLDCCNCC LDLANRCELQKEKSGESPGS PFVSNFRQLQEKLVFENLNT DKLNNIMRQDSMEPVVRDPC YLINEGICNRNIDQTMLSIL LFFH in isoform 3. 1 PublicationAdd BLAST40
Alternative sequenceiVSP_0550161 – 40MNTEM…VKLDN → MESQKASSAGAHLPAAPDLP EQAAAAAASKPEKMAQPKTE CRSPVGLDCCNCCLDLANRC ELQKEKSGESPGSPFVSNFR QLQEKLVFENLNTDKLNNIM RQDSMEPVVRDPCYLINEGI CNRNIDQTMLSILLFFH in isoform 4. 1 PublicationAdd BLAST40

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF024519 mRNA. Translation: AAD01789.1.
EU818782 mRNA. Translation: ACJ09091.1.
AF201287 mRNA. Translation: AAG41220.1.
AF201288 mRNA. Translation: AAG41221.1.
AF201289 mRNA. Translation: AAG41222.1.
AK083389 mRNA. Translation: BAC38897.1.
AK144196 mRNA. Translation: BAE25761.1.
AL683809 Genomic DNA. Translation: CAM24436.1.
BC028813 mRNA. Translation: AAH28813.1.
CCDSiCCDS30440.1. [Q9Z2S7-1]
CCDS41150.1. [Q9Z2S7-3]
RefSeqiNP_001070832.1. NM_001077364.1. [Q9Z2S7-3]
NP_034416.3. NM_010286.4. [Q9Z2S7-1]
XP_006528562.1. XM_006528499.3. [Q9Z2S7-3]
XP_006528563.1. XM_006528500.3. [Q9Z2S7-3]
XP_006528564.1. XM_006528501.3. [Q9Z2S7-1]
XP_006528566.1. XM_006528503.3. [Q9Z2S7-2]
XP_006528567.1. XM_006528504.3. [Q9Z2S7-3]
XP_006528568.1. XM_006528505.3. [Q9Z2S7-3]
UniGeneiMm.22216.

Genome annotation databases

EnsembliENSMUST00000055738; ENSMUSP00000062589; ENSMUSG00000031431. [Q9Z2S7-1]
ENSMUST00000112996; ENSMUSP00000108620; ENSMUSG00000031431. [Q9Z2S7-3]
GeneIDi14605.
KEGGimmu:14605.
UCSCiuc009ukz.2. mouse. [Q9Z2S7-1]
uc009ulb.1. mouse. [Q9Z2S7-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF024519 mRNA. Translation: AAD01789.1.
EU818782 mRNA. Translation: ACJ09091.1.
AF201287 mRNA. Translation: AAG41220.1.
AF201288 mRNA. Translation: AAG41221.1.
AF201289 mRNA. Translation: AAG41222.1.
AK083389 mRNA. Translation: BAC38897.1.
AK144196 mRNA. Translation: BAE25761.1.
AL683809 Genomic DNA. Translation: CAM24436.1.
BC028813 mRNA. Translation: AAH28813.1.
CCDSiCCDS30440.1. [Q9Z2S7-1]
CCDS41150.1. [Q9Z2S7-3]
RefSeqiNP_001070832.1. NM_001077364.1. [Q9Z2S7-3]
NP_034416.3. NM_010286.4. [Q9Z2S7-1]
XP_006528562.1. XM_006528499.3. [Q9Z2S7-3]
XP_006528563.1. XM_006528500.3. [Q9Z2S7-3]
XP_006528564.1. XM_006528501.3. [Q9Z2S7-1]
XP_006528566.1. XM_006528503.3. [Q9Z2S7-2]
XP_006528567.1. XM_006528504.3. [Q9Z2S7-3]
XP_006528568.1. XM_006528505.3. [Q9Z2S7-3]
UniGeneiMm.22216.

3D structure databases

ProteinModelPortaliQ9Z2S7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199919. 5 interactors.
DIPiDIP-48844N.
STRINGi10090.ENSMUSP00000108620.

PTM databases

iPTMnetiQ9Z2S7.
PhosphoSitePlusiQ9Z2S7.

2D gel databases

REPRODUCTION-2DPAGEIPI00265379.

Proteomic databases

PaxDbiQ9Z2S7.
PeptideAtlasiQ9Z2S7.
PRIDEiQ9Z2S7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000055738; ENSMUSP00000062589; ENSMUSG00000031431. [Q9Z2S7-1]
ENSMUST00000112996; ENSMUSP00000108620; ENSMUSG00000031431. [Q9Z2S7-3]
GeneIDi14605.
KEGGimmu:14605.
UCSCiuc009ukz.2. mouse. [Q9Z2S7-1]
uc009ulb.1. mouse. [Q9Z2S7-3]

Organism-specific databases

CTDi1831.
MGIiMGI:1196284. Tsc22d3.

Phylogenomic databases

eggNOGiENOG410IRR1. Eukaryota.
ENOG410YAFT. LUCA.
GeneTreeiENSGT00530000063062.
HOGENOMiHOG000015349.
HOVERGENiHBG075918.
InParanoidiQ9Z2S7.
OMAiSTEMFAK.
OrthoDBiEOG091G06PM.
PhylomeDBiQ9Z2S7.
TreeFamiTF329224.

Enzyme and pathway databases

ReactomeiR-MMU-2672351. Stimuli-sensing channels.

Miscellaneous databases

PROiQ9Z2S7.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031431.
CleanExiMM_TSC22D3.
ExpressionAtlasiQ9Z2S7. baseline and differential.
GenevisibleiQ9Z2S7. MM.

Family and domain databases

InterProiIPR000580. TSC-22_Dip_Bun.
[Graphical view]
PANTHERiPTHR12348. PTHR12348. 1 hit.
PfamiPF01166. TSC22. 1 hit.
[Graphical view]
ProDomiPD007152. TSC-22_Dip_Bun. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEiPS01289. TSC22. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiT22D3_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2S7
Secondary accession number(s): B1AVF3
, C6EX03, Q3UNI6, Q8K160, Q9EQN0, Q9EQN1, Q9EQN2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: February 1, 2005
Last modified: November 30, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.