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Q9Z2S7 (T22D3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
TSC22 domain family protein 3
Alternative name(s):
Glucocorticoid-induced leucine zipper protein
TSC22-related-inducible leucine zipper 3
Short name=Tilz3
Gene names
Name:Tsc22d3
Synonyms:Dsip1, Dsipi, Gilz
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length137 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Protects T-cells from IL2 deprivation-induced apoptosis through the inhibition of FOXO3A transcriptional activity that leads to the down-regulation of the pro-apoptotic factor BCL2L11. In macrophages, plays a role in the anti-inflammatory and immunosuppressive effects of glucocorticoids and IL10. In T-cells, inhibits anti-CD3-induced NFKB1 nuclear translocation. In vitro, suppresses AP1 and NFKB1 DNA-binding activities By similarity.

Subunit structure

Can form homodimers, however it is likely to function as a monomer. Interacts with AP1 and NFKB1 By similarity. Ref.7

Tissue specificity

Constitutively expressed in lung, intestine, kidney and liver, most probably by resident cells from the macrophage lineage. Expression inversely correlates with T-cell activation, being higher in resting cells and lower in cells activated by TCR/CD3 triggering. Ref.6 Ref.8

Induction

By glucocorticoids in lymphoid cells and upon IL4, IL10, IL13 or glucocorticoid treatment in monocyte/macrophage cells. Transiently induced by IL2 deprivation in T-cells By similarity. Ref.1 Ref.8 Ref.9

Domain

The leucine-zipper is involved in homodimerization.

Post-translational modification

Isoform 3 is phosphorylated on Ser-30 and Ser-40.

Sequence similarities

Belongs to the TSC-22/Dip/Bun family.

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: Q9Z2S7-1)

Also known as: Tilz3b;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: Q9Z2S7-2)

Also known as: Tilz3a;

The sequence of this isoform differs from the canonical sequence as follows:
     1-57: Missing.
Isoform 3 (identifier: Q9Z2S7-3)

Also known as: Tilz3c;

The sequence of this isoform differs from the canonical sequence as follows:
     1-40: MNTEMYQTPM...GDVVSVKLDN → MAQPKTECRS...TMLSILLFFH

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 137137TSC22 domain family protein 3
PRO_0000219371

Regions

Region1 – 6060AP1-binding
Region76 – 9722Leucine-zipper

Natural variations

Alternative sequence1 – 5757Missing in isoform 2.
VSP_012690
Alternative sequence1 – 4040MNTEM…VKLDN → MAQPKTECRSPVGLDCCNCC LDLANRCELQKEKSGESPGS PFVSNFRQLQEKLVFENLNT DKLNNIMRQDSMEPVVRDPC YLINEGICNRNIDQTMLSIL LFFH in isoform 3.
VSP_012691

Experimental info

Sequence conflict221I → T in AAD01789. Ref.1
Sequence conflict331V → L in AAG41221. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (Tilz3b) [UniParc].

Last modified February 1, 2005. Version 2.
Checksum: A11D7B69037F111E

FASTA13715,177
        10         20         30         40         50         60 
MNTEMYQTPM EVAVYQLHNF SISFFSSLLG GDVVSVKLDN SASGASVVAL DNKIEQAMDL 

        70         80         90        100        110        120 
VKNHLMYAVR EEVEVLKEQI RELLEKNSQL ERENTLLKTL ASPEQLEKFQ SRLSPEEPAP 

       130 
EAPETPETPE APGGSAV 

« Hide

Isoform 2 (Tilz3a) [UniParc].

Checksum: 2174BA0EC92DDCFC
Show »

FASTA809,040
Isoform 3 (Tilz3c) [UniParc].

Checksum: B12F0480154A20DE
Show »

FASTA20122,565

References

« Hide 'large scale' references
[1]"A new dexamethasone-induced gene of the leucine zipper family protects T lymphocytes from TCR/CD3-activated cell death."
D'Adamio F., Zollo O., Moraca R., Ayroldi E., Bruscoli S., Bartoli A., Cannarile L., Migliorati G., Riccardi C.
Immunity 7:803-812(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), INDUCTION.
Strain: C3H/HeN.
Tissue: Thymus.
[2]"Identification and characterization of a family of leucine zipper genes related to TSC22."
Ershler M.A., Belyavsky A.V., Visser J.W.M.
Submitted (NOV-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1; 2 AND 3).
[3]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: BALB/c and C57BL/6J.
Tissue: Thymus.
[4]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Liver.
[6]"Modulation of T-cell activation by the glucocorticoid-induced leucine zipper factor via inhibition of nuclear factor kappa B."
Ayroldi E., Migliorati G., Bruscoli S., Marchetti C., Zollo O., Cannarile L., D'Adamio F., Riccardi C.
Blood 98:743-753(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[7]"Inhibition of AP-1 by the glucocorticoid-inducible protein GILZ."
Mittelstadt P.R., Ashwell J.D.
J. Biol. Chem. 276:29603-29610(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: HOMODIMERIZATION, INTERACTION WITH JUN AND FOS.
[8]"Synthesis of glucocorticoid-induced leucine zipper (GILZ) by macrophages: an anti-inflammatory and immunosuppressive mechanism shared by glucocorticoids and IL-10."
Berrebi D., Bruscoli S., Cohen N., Foussat A., Migliorati G., Bouchet-Delbos L., Maillot M.-C., Portier A., Couderc J., Galanaud P., Peuchmaur M., Riccardi C., Emilie D.
Blood 101:729-738(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, INDUCTION.
[9]"GILZ, a new target for the transcription factor FoxO3, protects T lymphocytes from interleukin-2 withdrawal-induced apoptosis."
Asselin-Labat M.-L., David M., Biola-Vidamment A., Lecoeuche D., Zennaro M.-C., Bertoglio J., Pallardy M.
Blood 104:215-223(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF024519 mRNA. Translation: AAD01789.1.
AF201287 mRNA. Translation: AAG41220.1.
AF201288 mRNA. Translation: AAG41221.1.
AF201289 mRNA. Translation: AAG41222.1.
AK083389 mRNA. Translation: BAC38897.1.
AK144196 mRNA. Translation: BAE25761.1.
AL683809 Genomic DNA. Translation: CAM24436.1.
BC028813 mRNA. Translation: AAH28813.1.
RefSeqNP_001070832.1. NM_001077364.1.
NP_034416.3. NM_010286.3.
XP_006528562.1. XM_006528499.1.
XP_006528563.1. XM_006528500.1.
XP_006528564.1. XM_006528501.1.
XP_006528565.1. XM_006528502.1.
XP_006528566.1. XM_006528503.1.
XP_006528567.1. XM_006528504.1.
XP_006528568.1. XM_006528505.1.
UniGeneMm.22216.

3D structure databases

ProteinModelPortalQ9Z2S7.
SMRQ9Z2S7. Positions 58-114.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199919. 7 interactions.
DIPDIP-48844N.

PTM databases

PhosphoSiteQ9Z2S7.

2D gel databases

REPRODUCTION-2DPAGEIPI00265379.

Proteomic databases

PaxDbQ9Z2S7.
PRIDEQ9Z2S7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000055738; ENSMUSP00000062589; ENSMUSG00000031431. [Q9Z2S7-1]
ENSMUST00000112996; ENSMUSP00000108620; ENSMUSG00000031431. [Q9Z2S7-3]
GeneID14605.
KEGGmmu:14605.
UCSCuc009ukz.1. mouse. [Q9Z2S7-1]
uc009ulb.1. mouse. [Q9Z2S7-3]

Organism-specific databases

CTD1831.
MGIMGI:1196284. Tsc22d3.

Phylogenomic databases

eggNOGNOG329226.
GeneTreeENSGT00530000063062.
HOVERGENHBG075918.
InParanoidB1AVF3.
OMASTEMFAK.
OrthoDBEOG7RZ5PT.
PhylomeDBQ9Z2S7.
TreeFamTF329224.

Gene expression databases

ArrayExpressQ9Z2S7.
BgeeQ9Z2S7.
CleanExMM_TSC22D3.
GenevestigatorQ9Z2S7.

Family and domain databases

InterProIPR000580. TSC-22_Dip_Bun.
[Graphical view]
PANTHERPTHR12348. PTHR12348. 1 hit.
PfamPF01166. TSC22. 1 hit.
[Graphical view]
ProDomPD007152. TSC-22_Dip_Bun. 1 hit.
[Graphical view] [Entries sharing at least one domain]
PROSITEPS01289. TSC22. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio286388.
PROQ9Z2S7.
SOURCESearch...

Entry information

Entry nameT22D3_MOUSE
AccessionPrimary (citable) accession number: Q9Z2S7
Secondary accession number(s): B1AVF3 expand/collapse secondary AC list , Q3UNI6, Q8K160, Q9EQN0, Q9EQN1, Q9EQN2
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: February 1, 2005
Last modified: April 16, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot