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Q9Z2R6

- U119A_MOUSE

UniProt

Q9Z2R6 - U119A_MOUSE

Protein

Protein unc-119 homolog A

Gene

Unc119

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 85 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Myristoyl-binding protein that acts as a cargo adapter: specifically binds the myristoyl moiety of a subset of N-terminally myristoylated proteins and is required for their localization. Binds myristoylated GNAT1 and is required for G-protein localization and trafficking in sensory neurons. Binds myristoylated NPHP3; however, in contrast to UNC119B, does not seem to play a major role in ciliary membrane localization of NPHP3. Does not bind all myristoylated proteins. Probably plays a role in trafficking proteins in photoreceptor cells.2 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei131 – 1311LipidBy similarity

    GO - Molecular functioni

    1. lipid binding Source: UniProtKB
    2. protein binding Source: UniProtKB

    GO - Biological processi

    1. cytokinesis, completion of separation Source: UniProtKB
    2. lipoprotein transport Source: UniProtKB
    3. negative regulation of caveolin-mediated endocytosis Source: UniProtKB
    4. negative regulation of clathrin-mediated endocytosis Source: UniProtKB
    5. response to stimulus Source: UniProtKB-KW
    6. visual perception Source: UniProtKB-KW

    Keywords - Biological processi

    Endocytosis, Protein transport, Sensory transduction, Transport, Vision

    Keywords - Ligandi

    Lipid-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein unc-119 homolog A
    Alternative name(s):
    Retinal protein 4
    Short name:
    mRG4
    Gene namesi
    Name:Unc119
    Synonyms:Unc119h
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1328357. Unc119.

    Subcellular locationi

    Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity. Cytoplasmcytoskeletonspindle pole. Cytoplasmcytoskeletonspindle
    Note: Localizes to the centrosome in interphase cells and begins to translocate from the spindle pole to the spindle midzone after the onset of mitosis; it then localizes to the intercellular bridge in telophase cells and to the midbody in cytokinetic cells.By similarity

    GO - Cellular componenti

    1. centrosome Source: UniProtKB
    2. cytoplasm Source: UniProtKB-KW
    3. intercellular bridge Source: UniProtKB
    4. spindle midzone Source: UniProtKB
    5. spindle pole Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    Pathology & Biotechi

    Disruption phenotypei

    Mice develop a slowly progressive retinal degeneration, characterized by mottling in the fundus, mild thinning of the photoreceptor layer, and increase in apoptosis as early as 6 months, dramatic acceleration at approximately 17 months, and virtual obliteration of the photoreceptors by 20 months. Phenotypes are due to defects in protein trafficking, such as Gnat1 mislocalization.2 Publications

    Keywords - Diseasei

    Cone-rod dystrophy

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 240240Protein unc-119 homolog APRO_0000221213Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei37 – 371Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9Z2R6.
    PRIDEiQ9Z2R6.

    PTM databases

    PhosphoSiteiQ9Z2R6.

    Expressioni

    Tissue specificityi

    Localized in photoreceptor synapses in the outer plexiform layer of the retina.1 Publication

    Gene expression databases

    ArrayExpressiQ9Z2R6.
    BgeeiQ9Z2R6.
    CleanExiMM_UNC119.
    GenevestigatoriQ9Z2R6.

    Interactioni

    Subunit structurei

    Interacts with ARL1, ARL2 and ARL3 GTP-bound forms. Found in a complex with ARL3, RP2 and UNC119; RP2 induces hydrolysis of GTP ARL3 in the complex, leading to the release of UNC119. Interacts with NPHP3 (when myristoylated). Interacts with CYS1 (when myristoylated). Interacts with C5orf30; interaction only takes place when UNC119 is not liganded with myristoylated proteins By similarity. Interacts with CABP4; in the absence of calcium. Interacts with DNM1; leading to a decrease of DNM1 GTPase activity. Interacts with LCK; this interaction plays a crucial role in activation of LCK By similarity. Interacts with FYN By similarity. Interacts with RAB11A; in a cell cycle-dependent manner By similarity. Interacts with LYN (via SH2 and SH3 domains); leading to LYN activation By similarity. Found in a complex with ABL1, ABL2, CRK and UNC119; leading to the inhibition of CRK phosphorylation by ABL kinases. Interacts with CD44 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z2R6.
    SMRiQ9Z2R6. Positions 57-239.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domaini

    Adopts an immunoglobulin-like beta-sandwich fold forming a hydrophobic cavity that capture N-terminally myristoylated target peptides. Phe residues within the hydrophobic beta sandwich are required for myristate binding By similarity.By similarity

    Sequence similaritiesi

    Belongs to the PDE6D/unc-119 family.Curated

    Phylogenomic databases

    eggNOGiNOG305476.
    GeneTreeiENSGT00390000014595.
    HOGENOMiHOG000258286.
    HOVERGENiHBG108625.
    OrthoDBiEOG7288SP.
    PhylomeDBiQ9Z2R6.
    TreeFamiTF314474.

    Family and domain databases

    Gene3Di2.70.50.40. 1 hit.
    InterProiIPR008015. GMP_PDE_delta.
    IPR014756. Ig_E-set.
    [Graphical view]
    PfamiPF05351. GMP_PDE_delta. 1 hit.
    [Graphical view]
    SUPFAMiSSF81296. SSF81296. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q9Z2R6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKVKKGGGGT GSGAEPVPGA SNRSAEPTRE PGAEAESGSE SEPEPGPGPR    50
    LGPLQGKQPI GPEDVLGLQR ITGDYLCSPE ENIYKIDFVR FKIRDMDSGT 100
    VLFEIKKPPV SERLPINRRD LDPNAGRFVR YQFTPAFLRL RQVGATVEFT 150
    VGDKPVNNFR MIERHYFRNQ LLKSFDFHFG FCIPSSKNTC EHIYDFPPLS 200
    EELISEMIRH PYETQSDSFY FVDDRLVMHN KADYSYSGTP 240
    Length:240
    Mass (Da):27,010
    Last modified:May 1, 1999 - v1
    Checksum:i9EDF51191F0F5203
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF030169 mRNA. Translation: AAD01893.1.
    BC001990 mRNA. Translation: AAH01990.1.
    CCDSiCCDS25101.1.
    RefSeqiNP_035806.1. NM_011676.2.
    UniGeneiMm.284811.

    Genome annotation databases

    EnsembliENSMUST00000002127; ENSMUSP00000002127; ENSMUSG00000002058.
    GeneIDi22248.
    KEGGimmu:22248.
    UCSCiuc007kjb.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF030169 mRNA. Translation: AAD01893.1 .
    BC001990 mRNA. Translation: AAH01990.1 .
    CCDSi CCDS25101.1.
    RefSeqi NP_035806.1. NM_011676.2.
    UniGenei Mm.284811.

    3D structure databases

    ProteinModelPortali Q9Z2R6.
    SMRi Q9Z2R6. Positions 57-239.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q9Z2R6.

    Proteomic databases

    PaxDbi Q9Z2R6.
    PRIDEi Q9Z2R6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000002127 ; ENSMUSP00000002127 ; ENSMUSG00000002058 .
    GeneIDi 22248.
    KEGGi mmu:22248.
    UCSCi uc007kjb.1. mouse.

    Organism-specific databases

    CTDi 9094.
    MGIi MGI:1328357. Unc119.

    Phylogenomic databases

    eggNOGi NOG305476.
    GeneTreei ENSGT00390000014595.
    HOGENOMi HOG000258286.
    HOVERGENi HBG108625.
    OrthoDBi EOG7288SP.
    PhylomeDBi Q9Z2R6.
    TreeFami TF314474.

    Miscellaneous databases

    NextBioi 302323.
    PROi Q9Z2R6.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi Q9Z2R6.
    Bgeei Q9Z2R6.
    CleanExi MM_UNC119.
    Genevestigatori Q9Z2R6.

    Family and domain databases

    Gene3Di 2.70.50.40. 1 hit.
    InterProi IPR008015. GMP_PDE_delta.
    IPR014756. Ig_E-set.
    [Graphical view ]
    Pfami PF05351. GMP_PDE_delta. 1 hit.
    [Graphical view ]
    SUPFAMi SSF81296. SSF81296. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Mammalian orthologs of C. elegans unc-119 highly expressed in photoreceptors."
      Swanson D.A., Chang J.T., Campochiaro P.A., Zack D.J., Valle D.
      Invest. Ophthalmol. Vis. Sci. 39:2085-2094(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Retina.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Mammary gland.
    3. "Targeted inactivation of synaptic HRG4 (UNC119) causes dysfunction in the distal photoreceptor and slow retinal degeneration, revealing a new function."
      Ishiba Y., Higashide T., Mori N., Kobayashi A., Kubota S., McLaren M.J., Satoh H., Wong F., Inana G.
      Exp. Eye Res. 84:473-485(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-37, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    5. "Interaction and colocalization of CaBP4 and Unc119 (MRG4) in photoreceptors."
      Haeseleer F.
      Invest. Ophthalmol. Vis. Sci. 49:2366-2375(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH CABP4, TISSUE SPECIFICITY.
    6. "UNC119 inhibits dynamin and dynamin-dependent endocytic processes."
      Karim Z., Vepachedu R., Gorska M., Alam R.
      Cell. Signal. 22:128-137(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN ENDOCYTOSIS, INTERACTION WITH DNM1.
    7. Cited for: FUNCTION, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiU119A_MOUSE
    AccessioniPrimary (citable) accession number: Q9Z2R6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 85 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3