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Q9Z2R6 (U119A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Protein unc-119 homolog A
Alternative name(s):
Retinal protein 4
Short name=mRG4
Gene names
Name:Unc119
Synonyms:Unc119h
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length240 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Myristoyl-binding protein that acts as a cargo adapter: specifically binds the myristoyl moiety of a subset of N-terminally myristoylated proteins and is required for their localization. Binds myristoylated GNAT1 and is required for G-protein localization and trafficking in sensory neurons. Binds myristoylated NPHP3; however, in contrast to UNC119B, does not seem to play a major role in ciliary membrane localization of NPHP3. Does not bind all myristoylated proteins. Probably plays a role in trafficking proteins in photoreceptor cells. Ref.6

Subunit structure

Interacts with ARL1, ARL2 and ARL3 GTP-bound forms. Found in a complex with ARL3, RP2 and UNC119; RP2 induces hydrolysis of GTP ARL3 in the complex, leading to the release of UNC119. Interacts with NPHP3 (when myristoylated). Interacts with CYS1 (when myristoylated). Interacts with C5orf30; interaction only takes place when UNC119 is not liganded with myristoylated proteins By similarity. Interacts with CABP4; in the absence of calcium. Ref.5

Subcellular location

Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity.

Tissue specificity

Retinal-specific. Localized in photoreceptor synapses in the outer plexiform layer of the retina. Ref.5

Domain

Adopts an immunoglobulin-like beta-sandwich fold forming a hydrophobic cavity that capture N-terminally myristoylated target peptides. Phe residues within the hydrophobic beta sandwich are required for myristate binding By similarity.

Disruption phenotype

Mice develop a slowly progressive retinal degeneration, characterized by mottling in the fundus, mild thinning of the photoreceptor layer, and increase in apoptosis as early as 6 months, dramatic acceleration at approximately 17 months, and virtual obliteration of the photoreceptors by 20 months. Phenotypes are due to defects in protein trafficking, such as Gnat1 mislocalization. Ref.3 Ref.6

Sequence similarities

Belongs to the PDE6D/unc-119 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 240240Protein unc-119 homolog A
PRO_0000221213

Sites

Binding site1311Lipid By similarity

Amino acid modifications

Modified residue371Phosphoserine Ref.4

Sequences

Sequence LengthMass (Da)Tools
Q9Z2R6 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 9EDF51191F0F5203

FASTA24027,010
        10         20         30         40         50         60 
MKVKKGGGGT GSGAEPVPGA SNRSAEPTRE PGAEAESGSE SEPEPGPGPR LGPLQGKQPI 

        70         80         90        100        110        120 
GPEDVLGLQR ITGDYLCSPE ENIYKIDFVR FKIRDMDSGT VLFEIKKPPV SERLPINRRD 

       130        140        150        160        170        180 
LDPNAGRFVR YQFTPAFLRL RQVGATVEFT VGDKPVNNFR MIERHYFRNQ LLKSFDFHFG 

       190        200        210        220        230        240 
FCIPSSKNTC EHIYDFPPLS EELISEMIRH PYETQSDSFY FVDDRLVMHN KADYSYSGTP 

« Hide

References

« Hide 'large scale' references
[1]"Mammalian orthologs of C. elegans unc-119 highly expressed in photoreceptors."
Swanson D.A., Chang J.T., Campochiaro P.A., Zack D.J., Valle D.
Invest. Ophthalmol. Vis. Sci. 39:2085-2094(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Retina.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Mammary gland.
[3]"Targeted inactivation of synaptic HRG4 (UNC119) causes dysfunction in the distal photoreceptor and slow retinal degeneration, revealing a new function."
Ishiba Y., Higashide T., Mori N., Kobayashi A., Kubota S., McLaren M.J., Satoh H., Wong F., Inana G.
Exp. Eye Res. 84:473-485(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
[4]"Large-scale phosphorylation analysis of mouse liver."
Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-37, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Liver.
[5]"Interaction and colocalization of CaBP4 and Unc119 (MRG4) in photoreceptors."
Haeseleer F.
Invest. Ophthalmol. Vis. Sci. 49:2366-2375(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CABP4, TISSUE SPECIFICITY.
[6]"UNC119 is required for G protein trafficking in sensory neurons."
Zhang H., Constantine R., Vorobiev S., Chen Y., Seetharaman J., Huang Y.J., Xiao R., Montelione G.T., Gerstner C.D., Davis M.W., Inana G., Whitby F.G., Jorgensen E.M., Hill C.P., Tong L., Baehr W.
Nat. Neurosci. 14:874-880(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF030169 mRNA. Translation: AAD01893.1.
BC001990 mRNA. Translation: AAH01990.1.
RefSeqNP_035806.1. NM_011676.2.
UniGeneMm.284811.

3D structure databases

ProteinModelPortalQ9Z2R6.
SMRQ9Z2R6. Positions 57-239.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ9Z2R6.

Proteomic databases

PaxDbQ9Z2R6.
PRIDEQ9Z2R6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000002127; ENSMUSP00000002127; ENSMUSG00000002058.
GeneID22248.
KEGGmmu:22248.
UCSCuc007kjb.1. mouse.

Organism-specific databases

CTD9094.
MGIMGI:1328357. Unc119.

Phylogenomic databases

eggNOGNOG305476.
GeneTreeENSGT00390000014595.
HOGENOMHOG000258286.
HOVERGENHBG108625.
OrthoDBEOG7288SP.
PhylomeDBQ9Z2R6.
TreeFamTF314474.

Gene expression databases

ArrayExpressQ9Z2R6.
BgeeQ9Z2R6.
CleanExMM_UNC119.
GenevestigatorQ9Z2R6.

Family and domain databases

Gene3D2.70.50.40. 1 hit.
InterProIPR008015. GMP_PDE_delta.
IPR014756. Ig_E-set.
[Graphical view]
PfamPF05351. GMP_PDE_delta. 1 hit.
[Graphical view]
SUPFAMSSF81296. SSF81296. 1 hit.
ProtoNetSearch...

Other

NextBio302323.
PROQ9Z2R6.
SOURCESearch...

Entry information

Entry nameU119A_MOUSE
AccessionPrimary (citable) accession number: Q9Z2R6
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: April 16, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot