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Protein

Protein unc-119 homolog A

Gene

Unc119

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in synaptic functions in photoreceptor cells, the signal transduction in immune cells as a Src family kinase activator, endosome recycling, the uptake of bacteria and endocytosis, protein trafficking in sensory neurons and as lipid-binding chaperone with specificity for a diverse subset of myristoylated proteins. Specifically binds the myristoyl moiety of a subset of N-terminally myristoylated proteins and is required for their localization. Binds myristoylated GNAT1 and is required for G-protein localization and trafficking in sensory neurons (PubMed:21642972). Probably plays a role in trafficking proteins in photoreceptor cells (PubMed:17174953). Plays important roles in mediating Src family kinase signals for the completion of cytokinesis via RAB11A.By similarity3 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei131 – 1311LipidBy similarity

GO - Molecular functioni

  1. lipid binding Source: UniProtKB

GO - Biological processi

  1. endocytosis Source: UniProtKB-KW
  2. lipoprotein transport Source: UniProtKB
  3. negative regulation of caveolin-mediated endocytosis Source: UniProtKB
  4. negative regulation of clathrin-mediated endocytosis Source: UniProtKB
  5. positive regulation of protein tyrosine kinase activity Source: MGI
  6. response to stimulus Source: UniProtKB-KW
  7. visual perception Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Endocytosis, Protein transport, Sensory transduction, Transport, Vision

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Protein unc-119 homolog A
Alternative name(s):
Retinal protein 4
Short name:
mRG4
Gene namesi
Name:Unc119
Synonyms:Unc119h
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1328357. Unc119.

Subcellular locationi

  1. Cytoplasmcytoskeletonmicrotubule organizing centercentrosome By similarity
  2. Cytoplasmcytoskeletonspindle By similarity
  3. Cytoplasmcytoskeletonspindle pole By similarity

  4. Note: ocalizes to the centrosome in interphase cells and begins to translocate from the spindle pole to the spindle midzone after the onset of mitosis; it then localizes to the intercellular bridge in telophase cells and to the midbody in cytokinetic cells.By similarity

GO - Cellular componenti

  1. centrosome Source: UniProtKB
  2. cytoplasm Source: UniProtKB-KW
  3. intercellular bridge Source: UniProtKB
  4. spindle midzone Source: UniProtKB
  5. spindle pole Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Disruption phenotypei

Mice develop a slowly progressive retinal degeneration, characterized by mottling in the fundus, mild thinning of the photoreceptor layer, and increase in apoptosis as early as 6 months, dramatic acceleration at approximately 17 months, and virtual obliteration of the photoreceptors by 20 months. Phenotypes are due to defects in protein trafficking, such as Gnat1 mislocalization.2 Publications

Keywords - Diseasei

Cone-rod dystrophy

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 240240Protein unc-119 homolog APRO_0000221213Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei37 – 371Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9Z2R6.
PaxDbiQ9Z2R6.
PRIDEiQ9Z2R6.

PTM databases

PhosphoSiteiQ9Z2R6.

Expressioni

Tissue specificityi

Localized in photoreceptor synapses in the outer plexiform layer of the retina.1 Publication

Gene expression databases

BgeeiQ9Z2R6.
CleanExiMM_UNC119.
ExpressionAtlasiQ9Z2R6. baseline and differential.
GenevestigatoriQ9Z2R6.

Interactioni

Subunit structurei

May interact with GTP-bound ARL1. Interacts with ARL2 and ARL3 (GTP-bound forms); this promotes the release of myristoylated cargo proteins (By similarity). Found in a complex with ARL3, RP2 and UNC119; RP2 induces hydrolysis of GTP ARL3 in the complex, leading to the release of UNC119. Interacts with NPHP3 (when myristoylated). Interacts with CYS1 (when myristoylated). Interacts with C5orf30; interaction only takes place when UNC119 is not liganded with myristoylated proteins (By similarity). Interacts with CABP4; in the absence of calcium. Interacts with DNM1; leading to a decrease of DNM1 GTPase activity. Interacts with LCK; this interaction plays a crucial role in activation of LCK (By similarity). Interacts with FYN (By similarity). Interacts with RAB11A; in a cell cycle-dependent manner (By similarity). Interacts with LYN (via SH2 and SH3 domains); leading to LYN activation (By similarity). Found in a complex with ABL1, ABL2, CRK and UNC119; leading to the inhibition of CRK phosphorylation by ABL kinases. Interacts with CD44 (By similarity).By similarity

Structurei

3D structure databases

SMRiQ9Z2R6. Positions 57-239.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Adopts an immunoglobulin-like beta-sandwich fold forming a hydrophobic cavity that captures N-terminally myristoylated target peptides. Phe residues within the hydrophobic beta sandwich are required for myristate binding (By similarity).By similarity

Sequence similaritiesi

Belongs to the PDE6D/unc-119 family.Curated

Phylogenomic databases

eggNOGiNOG305476.
GeneTreeiENSGT00390000014595.
HOGENOMiHOG000258286.
HOVERGENiHBG108625.
InParanoidiQ9Z2R6.
OrthoDBiEOG7288SP.
PhylomeDBiQ9Z2R6.
TreeFamiTF314474.

Family and domain databases

Gene3Di2.70.50.40. 1 hit.
InterProiIPR008015. GMP_PDE_delta.
IPR014756. Ig_E-set.
[Graphical view]
PfamiPF05351. GMP_PDE_delta. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 1 hit.

Sequencei

Sequence statusi: Complete.

Q9Z2R6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVKKGGGGT GSGAEPVPGA SNRSAEPTRE PGAEAESGSE SEPEPGPGPR
60 70 80 90 100
LGPLQGKQPI GPEDVLGLQR ITGDYLCSPE ENIYKIDFVR FKIRDMDSGT
110 120 130 140 150
VLFEIKKPPV SERLPINRRD LDPNAGRFVR YQFTPAFLRL RQVGATVEFT
160 170 180 190 200
VGDKPVNNFR MIERHYFRNQ LLKSFDFHFG FCIPSSKNTC EHIYDFPPLS
210 220 230 240
EELISEMIRH PYETQSDSFY FVDDRLVMHN KADYSYSGTP
Length:240
Mass (Da):27,010
Last modified:May 1, 1999 - v1
Checksum:i9EDF51191F0F5203
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030169 mRNA. Translation: AAD01893.1.
BC001990 mRNA. Translation: AAH01990.1.
CCDSiCCDS25101.1.
RefSeqiNP_035806.1. NM_011676.2.
UniGeneiMm.284811.

Genome annotation databases

EnsembliENSMUST00000002127; ENSMUSP00000002127; ENSMUSG00000002058.
GeneIDi22248.
KEGGimmu:22248.
UCSCiuc007kjb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030169 mRNA. Translation: AAD01893.1.
BC001990 mRNA. Translation: AAH01990.1.
CCDSiCCDS25101.1.
RefSeqiNP_035806.1. NM_011676.2.
UniGeneiMm.284811.

3D structure databases

SMRiQ9Z2R6. Positions 57-239.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ9Z2R6.

Proteomic databases

MaxQBiQ9Z2R6.
PaxDbiQ9Z2R6.
PRIDEiQ9Z2R6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002127; ENSMUSP00000002127; ENSMUSG00000002058.
GeneIDi22248.
KEGGimmu:22248.
UCSCiuc007kjb.1. mouse.

Organism-specific databases

CTDi9094.
MGIiMGI:1328357. Unc119.

Phylogenomic databases

eggNOGiNOG305476.
GeneTreeiENSGT00390000014595.
HOGENOMiHOG000258286.
HOVERGENiHBG108625.
InParanoidiQ9Z2R6.
OrthoDBiEOG7288SP.
PhylomeDBiQ9Z2R6.
TreeFamiTF314474.

Miscellaneous databases

NextBioi302323.
PROiQ9Z2R6.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Z2R6.
CleanExiMM_UNC119.
ExpressionAtlasiQ9Z2R6. baseline and differential.
GenevestigatoriQ9Z2R6.

Family and domain databases

Gene3Di2.70.50.40. 1 hit.
InterProiIPR008015. GMP_PDE_delta.
IPR014756. Ig_E-set.
[Graphical view]
PfamiPF05351. GMP_PDE_delta. 1 hit.
[Graphical view]
SUPFAMiSSF81296. SSF81296. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mammalian orthologs of C. elegans unc-119 highly expressed in photoreceptors."
    Swanson D.A., Chang J.T., Campochiaro P.A., Zack D.J., Valle D.
    Invest. Ophthalmol. Vis. Sci. 39:2085-2094(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Retina.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Mammary gland.
  3. "Targeted inactivation of synaptic HRG4 (UNC119) causes dysfunction in the distal photoreceptor and slow retinal degeneration, revealing a new function."
    Ishiba Y., Higashide T., Mori N., Kobayashi A., Kubota S., McLaren M.J., Satoh H., Wong F., Inana G.
    Exp. Eye Res. 84:473-485(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-37, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. "Interaction and colocalization of CaBP4 and Unc119 (MRG4) in photoreceptors."
    Haeseleer F.
    Invest. Ophthalmol. Vis. Sci. 49:2366-2375(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CABP4, TISSUE SPECIFICITY.
  6. "UNC119 inhibits dynamin and dynamin-dependent endocytic processes."
    Karim Z., Vepachedu R., Gorska M., Alam R.
    Cell. Signal. 22:128-137(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN ENDOCYTOSIS, INTERACTION WITH DNM1.
  7. Cited for: FUNCTION, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiU119A_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2R6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: May 1, 1999
Last modified: April 1, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.