Syntaxin-8 - Rattus norvegicus (Rat)

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Q9Z2Q7 (STX8_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 87. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Syntaxin-8

Gene names

Name:

Stx8

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Vesicle trafficking protein that functions in the early secretory pathway, possibly by mediating retrograde transport from cis-Golgi membranes to the ER.
Subunit structure	Part of the SNARE core complex containing STX7, VAMP8 and VTI1B. Interacts with VAMP8. Forms a SNARE complex with STX7, VTI1B and VAMP8 which functions in the homotypic fusion of late endosomes. Component of the SNARE complex composed of STX7, STX8, VAMP7 and VTI1B that is required for heterotypic fusion of late endosomes with lysosomes in liver cells. Interacts with HECTD3 By similarity. Ref.3
Subcellular location	Membrane; Single-pass type IV membrane protein. Note: Preferentially associated with the early endosome. To lesser extends, also present in late endosome, the plasma membrane and coated pits.
Tissue specificity	Widely expressed in all tissues examined.
Post-translational modification	Ubiquitinated by HECTD3 By similarity.
Sequence similarities	Belongs to the syntaxin family. Contains 1 t-SNARE coiled-coil homology domain.

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Domain	Coiled coil Transmembrane Transmembrane helix
PTM	Ubl conjugation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	endosome to lysosome transport Inferred from direct assay Ref.4. Source: RGD vesicle fusion Inferred from direct assay Ref.4. Source: RGD
Cellular component	SNARE complex Inferred from direct assay Ref.4. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW late endosome membrane Inferred from direct assay Ref.4. Source: RGD lysosomal membrane Inferred from direct assay Ref.4. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 236

236

Syntaxin-8

PRO_0000210219

Regions

Topological domain

1 – 215

215

Cytoplasmic Potential

Transmembrane

216 – 232

Helical; Anchor for type IV membrane protein; Potential

Topological domain

233 – 236

Vesicular Potential

Domain

145 – 207

t-SNARE coiled-coil homology

Coiled coil

42 – 65

By similarity

Secondary structure

236

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

Q9Z2Q7 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: E86A9CD14618E197
Show »

FASTA

236

26,911

        10         20         30         40         50         60 
MAPDPWFSTY DSTCQIAQEI AEKIQERNQC ERRGEKTPKL TLTIRTLLKN LKVKIDLLKD 

        70         80         90        100        110        120 
LLLRAVSTRQ ITQLEGDRRQ NLLDDLVTRE RLLLASFKNE GSEPDLIRSS LMSEEAKRGT 

       130        140        150        160        170        180 
PNPWLCEEPE ETRGLGFDEI RQQQQKIIQE QDAGLDALSS IISRQKQMGQ EIGNELDEQN 

       190        200        210        220        230 
EIIDDLANLV ENTDEKLRTE ARRVTLVDRK SASCGMIMVI LLLLVAIVVV AVWPTN

« Hide

References

[1]	"Preferential association of syntaxin 8 with the early endosome." Subramaniam V.N., Loh E., Horstmann H., Habermann A., Xu Y., Coe J., Griffiths G., Hong W. J. Cell Sci. 113:997-1008(2000) [PubMed: 10683148] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"A SNARE complex mediating fusion of late endosomes defines conserved properties of SNARE structure and function." Antonin W., Holroyd C., Fasshauer D., Pabst S., Fischer von Mollard G., Jahn R. EMBO J. 19:6453-6464(2000) [PubMed: 11101518] [Abstract] Cited for: SNARE COMPLEX CHARACTERIZATION.
[3]	"Crystal structure of the endosomal SNARE complex reveals common structural principles of all SNAREs." Antonin W., Fasshauer D., Becker S., Jahn R., Schneider T.R. Nat. Struct. Biol. 9:107-111(2002) [PubMed: 11786915] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 149-209 IN COMPLEX WITH STX7; VAMP8 AND VTI1B, INTERACTION WITH VAMP8.
[4]	"Combinatorial SNARE complexes with VAMP7 or VAMP8 define different late endocytic fusion events." Pryor P.R., Mullock B.M., Bright N.A., Lindsay M.R., Gray S.R., Richardson S.C.W., Stewart A., James D.E., Piper R.C., Luzio J.P. EMBO Rep. 5:590-595(2004) [PubMed: 15133481] [Abstract] Cited for: SNARE COMPLEX CHARACTERIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF033109 mRNA. Translation: AAC70903.1.

IPI

IPI00210157.

RefSeq

NP_113844.1. NM_031656.1.

UniGene

Rn.20291.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GL2	X-ray	1.90	D	149-209	[»]

ProteinModelPortal

Q9Z2Q7.

SMR

Q9Z2Q7. Positions 152-205.

ModBase

Search...

Protein-protein interaction databases

STRING

Q9Z2Q7.

Proteomic databases

PRIDE

Q9Z2Q7.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSRNOT00000005204; ENSRNOP00000005204; ENSRNOG00000003849.

GeneID

59074.

KEGG

rno:59074.

UCSC

NM_031656. rat.

Organism-specific databases

CTD

9482.

RGD

61917. Stx8.

Phylogenomic databases

eggNOG

roNOG06719.

GeneTree

ENSGT00390000007779.

HOVERGEN

HBG007572.

InParanoid

Q9Z2Q7.

OMA

QKIIQEQ.

OrthoDB

EOG402WT8.

PhylomeDB

Q9Z2Q7.

Gene expression databases

ArrayExpress

Q9Z2Q7.

Genevestigator

Q9Z2Q7.

GermOnline

ENSRNOG00000003849. Rattus norvegicus.

Family and domain databases

InterPro

IPR015640. Syntax_8.
IPR000727. T_SNARE_dom.
[Graphical view]

K08501.

PANTHER

PTHR12380:SF6. Syntax_8. 1 hit.

Pfam

PF05739. SNARE. 1 hit.
[Graphical view]

SMART

SM00397. t_SNARE. 1 hit.
[Graphical view]

PROSITE

PS00914. SYNTAXIN. False negative.
PS50192. T_SNARE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

611688.

Entry information

Entry name

STX8_RAT

Accession

Primary (citable) accession number: Q9Z2Q7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2001
Last sequence update:	May 1, 1999
Last modified:	November 16, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents