Q9Z2Q3 (HYAL2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hyaluronidase-2
Short name=Hyal-2
EC=3.2.1.35

Alternative name(s):
Hyaluronoglucosaminidase-2

Gene names

Name:

Hyal2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R By similarity.
Catalytic activity	Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.
Subunit structure	Interacts with MST1R By similarity.
Subcellular location	Cell membrane; Lipid-anchor › GPI-anchor By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 56 family. Contains 1 EGF-like domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	EGF-like domain Signal
Molecular function	Glycosidase Hydrolase Receptor
PTM	Disulfide bond GPI-anchor Glycoprotein Lipoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cartilage development Inferred from sequence or structural similarity. Source: UniProtKB cellular response to UV-B Inferred from sequence or structural similarity. Source: UniProtKB cellular response to fibroblast growth factor stimulus Inferred from sequence or structural similarity. Source: UniProtKB cellular response to interleukin-1 Inferred from sequence or structural similarity. Source: UniProtKB cellular response to transforming growth factor beta stimulus Inferred from sequence or structural similarity. Source: UniProtKB hyaluronan catabolic process Inferred from direct assay PubMed 19635555. Source: UniProtKB kidney development Inferred from expression pattern PubMed 17706761. Source: UniProtKB monocyte activation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of MAP kinase activity Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of cell growth Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of fibroblast migration Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of protein kinase B signaling cascade Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of inflammatory response Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of interleukin-6 secretion Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of interleukin-8 secretion Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of urine volume Inferred from expression pattern PubMed 19635555. Source: UniProtKB renal water absorption Inferred from expression pattern PubMed 19635555. Source: UniProtKB response to reactive oxygen species Inferred from sequence or structural similarity. Source: UniProtKB transformation of host cell by virus Inferred from direct assay PubMed 12584308. Source: RGD viral entry into host cell via membrane fusion with the plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB
Cellular_component	Golgi membrane Inferred from direct assay PubMed 21740893. Source: UniProtKB anchored to plasma membrane Inferred from direct assay PubMed 21740893. Source: UniProtKB apical plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB cytosol Inferred from sequence or structural similarity. Source: UniProtKB endocytic vesicle Inferred from sequence or structural similarity. Source: UniProtKB lysosome Inferred from sequence or structural similarity. Source: UniProtKB membrane raft Inferred from direct assay PubMed 21740893. Source: UniProtKB microvillus Inferred from sequence or structural similarity. Source: UniProtKB perinuclear region of cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular_function	hyaluronic acid binding Inferred from sequence or structural similarity. Source: UniProtKB hyaluronoglucuronidase activity Inferred from direct assay PubMed 19635555. Source: UniProtKB hyalurononglucosaminidase activity Inferred from sequence or structural similarity. Source: UniProtKB receptor signaling protein tyrosine kinase inhibitor activity Inferred from sequence or structural similarity. Source: UniProtKB viral receptor activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 448	428	Hyaluronidase-2		PRO_0000012103
	Propeptide	449 – 473	25	Removed in mature form Potential		PRO_0000012104
Regions
	Domain	361 – 439	79	EGF-like
Sites
	Active site	135	1	Proton donor By similarity
Amino acid modifications
	Lipidation	448	1	GPI-anchor amidated aspartate Potential
	Glycosylation	74	1	N-linked (GlcNAc...) Potential
	Glycosylation	103	1	N-linked (GlcNAc...) Potential
	Glycosylation	357	1	N-linked (GlcNAc...) Potential
	Disulfide bond	47 ↔ 340		By similarity
	Disulfide bond	211 ↔ 227		By similarity
	Disulfide bond	365 ↔ 376		By similarity
	Disulfide bond	370 ↔ 427		By similarity
	Disulfide bond	429 ↔ 438		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q9Z2Q3 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 46B90A2F260B0CE4	FASTA	473	53,999
	10 20 30 40 50 60 MRAGLGPIIT LALVLEVAWA SELKPTAPPI FTGRPFVVAW NVPTQECAPR HKVPLDLRAF 70 80 90 100 110 120 DVEATPNEGF FNQNITTFYY DRLGLYPRFD AAGMSVHGGV PQNGSLCAHL PMLKEAVERY 130 140 150 160 170 180 IQTQEPAGLA VIDWEEWRPV WVRNWQEKDV YRQSSRQLVA SRHPDWPSDR IVKQAQYEFE 190 200 210 220 230 240 FAARQFMLNT LRYVKAVRPQ HLWGFYLFPD CYNHDYVQNW DSYTGRCPDV EVAQNDQLAW 250 260 270 280 290 300 LWAENTALFP SVYLDKTLAS SKHSRNFVSF RVQEALRVAH THHANHALPV YVFTRPTYTR 310 320 330 340 350 360 RLTELNQMDL ISTIGESAAL GSAGVIFWGD SVYASSMENC QNLKKYLTQT LVPYIVNVSW 370 380 390 400 410 420 ATQYCSWTQC HGHGRCVRRN PSASTFLHLS PSSFRLVPGR TPSEPQLRPE GELSEDDLSY 430 440 450 460 470 LQMHFRCHCY LGWGGEQCQW NHKRAAGDAS RAWAGAHLAS LLGLVAMTLT WTL

References

[1]	"Cloning and expression of the cDNA for a rat hyaluronidase." Boel P., Mertens-Strijthagen J., Flamion B. Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF034218 mRNA. Translation: AAD01980.1.
IPI	IPI00210151.
UniGene	Rn.8278.
3D structure databases
ProteinModelPortal	Q9Z2Q3.
ModBase	Search...
Protein family/group databases
CAZy	GH56. Glycoside Hydrolase Family 56.
Proteomic databases
PaxDb	Q9Z2Q3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
UCSC	RGD:620321. rat.
Organism-specific databases
RGD	620321. Hyal2.
Phylogenomic databases
eggNOG	NOG77606.
HOGENOM	HOG000015133.
HOVERGEN	HBG052053.
InParanoid	Q9Z2Q3.
OrthoDB	EOG4H463X.
Gene expression databases
Genevestigator	Q9Z2Q3.
GermOnline	ENSRNOG00000031420. Rattus norvegicus.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
InterPro	IPR013785. Aldolase_TIM. IPR000742. EG-like_dom. IPR017853. Glycoside_hydrolase_SF. IPR018155. Hyaluronidase. [Graphical view]
PANTHER	PTHR11769. PTHR11769. 1 hit.
Pfam	PF01630. Glyco_hydro_56. 1 hit. [Graphical view]
PIRSF	PIRSF038193. Hyaluronidase. 1 hit.
PRINTS	PR00846. GLHYDRLASE56.
SMART	SM00181. EGF. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00022. EGF_1. 1 hit. PS01186. EGF_2. 1 hit. PS50026. EGF_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HYAL2_RAT

Accession

Primary (citable) accession number: Q9Z2Q3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	May 1, 1999
Last modified:	April 3, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program