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Protein

Hyaluronidase-2

Gene

Hyal2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product which is further hydrolyzed by sperm hyaluronidase to give small oligosaccharides. Displays very low levels of activity. Associates with and negatively regulates MST1R (By similarity).By similarity

Catalytic activityi

Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei135 – 1351Proton donorBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase, Receptor

Enzyme and pathway databases

BRENDAi3.2.1.35. 5301.

Protein family/group databases

CAZyiGH56. Glycoside Hydrolase Family 56.

Names & Taxonomyi

Protein namesi
Recommended name:
Hyaluronidase-2 (EC:3.2.1.35)
Short name:
Hyal-2
Alternative name(s):
Hyaluronoglucosaminidase-2
Gene namesi
Name:Hyal2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620321. Hyal2.

Subcellular locationi

GO - Cellular componenti

  • anchored component of plasma membrane Source: UniProtKB
  • apical plasma membrane Source: UniProtKB
  • cytoplasmic membrane-bounded vesicle Source: UniProtKB
  • cytoplasmic vesicle Source: UniProtKB
  • cytosol Source: UniProtKB
  • endocytic vesicle Source: UniProtKB
  • Golgi membrane Source: UniProtKB
  • lysosome Source: UniProtKB
  • membrane raft Source: UniProtKB
  • microvillus Source: UniProtKB
  • perinuclear region of cytoplasm Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 448428Hyaluronidase-2PRO_0000012103Add
BLAST
Propeptidei449 – 47325Removed in mature formSequence AnalysisPRO_0000012104Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi47 ↔ 340By similarity
Glycosylationi74 – 741N-linked (GlcNAc...)Sequence Analysis
Glycosylationi103 – 1031N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi211 ↔ 227By similarity
Glycosylationi357 – 3571N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi365 ↔ 376By similarity
Disulfide bondi370 ↔ 427By similarity
Disulfide bondi429 ↔ 438By similarity
Lipidationi448 – 4481GPI-anchor amidated aspartateSequence Analysis

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiQ9Z2Q3.
PRIDEiQ9Z2Q3.

Interactioni

Subunit structurei

Interacts with MST1R.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017461.

Structurei

3D structure databases

ProteinModelPortaliQ9Z2Q3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini361 – 43979EGF-likeAdd
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 56 family.Curated
Contains 1 EGF-like domain.Curated

Keywords - Domaini

EGF-like domain, Signal

Phylogenomic databases

eggNOGiNOG77606.
HOGENOMiHOG000015133.
HOVERGENiHBG052053.
InParanoidiQ9Z2Q3.
PhylomeDBiQ9Z2Q3.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000742. EG-like_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PIRSFiPIRSF038193. Hyaluronidase. 1 hit.
PRINTSiPR00846. GLHYDRLASE56.
SMARTiSM00181. EGF. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z2Q3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAGLGPIIT LALVLEVAWA SELKPTAPPI FTGRPFVVAW NVPTQECAPR
60 70 80 90 100
HKVPLDLRAF DVEATPNEGF FNQNITTFYY DRLGLYPRFD AAGMSVHGGV
110 120 130 140 150
PQNGSLCAHL PMLKEAVERY IQTQEPAGLA VIDWEEWRPV WVRNWQEKDV
160 170 180 190 200
YRQSSRQLVA SRHPDWPSDR IVKQAQYEFE FAARQFMLNT LRYVKAVRPQ
210 220 230 240 250
HLWGFYLFPD CYNHDYVQNW DSYTGRCPDV EVAQNDQLAW LWAENTALFP
260 270 280 290 300
SVYLDKTLAS SKHSRNFVSF RVQEALRVAH THHANHALPV YVFTRPTYTR
310 320 330 340 350
RLTELNQMDL ISTIGESAAL GSAGVIFWGD SVYASSMENC QNLKKYLTQT
360 370 380 390 400
LVPYIVNVSW ATQYCSWTQC HGHGRCVRRN PSASTFLHLS PSSFRLVPGR
410 420 430 440 450
TPSEPQLRPE GELSEDDLSY LQMHFRCHCY LGWGGEQCQW NHKRAAGDAS
460 470
RAWAGAHLAS LLGLVAMTLT WTL
Length:473
Mass (Da):53,999
Last modified:May 1, 1999 - v1
Checksum:i46B90A2F260B0CE4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF034218 mRNA. Translation: AAD01980.1.
UniGeneiRn.8278.

Genome annotation databases

UCSCiRGD:620321. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF034218 mRNA. Translation: AAD01980.1.
UniGeneiRn.8278.

3D structure databases

ProteinModelPortaliQ9Z2Q3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000017461.

Protein family/group databases

CAZyiGH56. Glycoside Hydrolase Family 56.

Proteomic databases

PaxDbiQ9Z2Q3.
PRIDEiQ9Z2Q3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

UCSCiRGD:620321. rat.

Organism-specific databases

RGDi620321. Hyal2.

Phylogenomic databases

eggNOGiNOG77606.
HOGENOMiHOG000015133.
HOVERGENiHBG052053.
InParanoidiQ9Z2Q3.
PhylomeDBiQ9Z2Q3.

Enzyme and pathway databases

BRENDAi3.2.1.35. 5301.

Miscellaneous databases

PROiQ9Z2Q3.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR000742. EG-like_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR018155. Hyaluronidase.
[Graphical view]
PANTHERiPTHR11769. PTHR11769. 1 hit.
PfamiPF01630. Glyco_hydro_56. 1 hit.
[Graphical view]
PIRSFiPIRSF038193. Hyaluronidase. 1 hit.
PRINTSiPR00846. GLHYDRLASE56.
SMARTiSM00181. EGF. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and expression of the cDNA for a rat hyaluronidase."
    Boel P., Mertens-Strijthagen J., Flamion B.
    Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiHYAL2_RAT
AccessioniPrimary (citable) accession number: Q9Z2Q3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 15, 2002
Last sequence update: May 1, 1999
Last modified: June 24, 2015
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.