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Protein

Protein transport protein Sec31A

Gene

Sec31a

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the coat protein complex II (COPII) which promotes the formation of transport vesicles from the endoplasmic reticulum (ER). The coat has two main functions, the physical deformation of the endoplasmic reticulum membrane into vesicles and the selection of cargo molecules.1 Publication

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

ER-Golgi transport, Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-RNO-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-RNO-2132295. MHC class II antigen presentation.
R-RNO-983170. Antigen Presentation: Folding, assembly and peptide loading of class I MHC.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein transport protein Sec31A
Alternative name(s):
SEC31-like protein 1
SEC31-related protein A
Vesicle-associated protein 1
Gene namesi
Name:Sec31a
Synonyms:Sec31l1, Vap1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 14

Organism-specific databases

RGDi620233. Sec31a.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12491249Protein transport protein Sec31APRO_0000295150Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei526 – 5261PhosphoserineCombined sources
Modified residuei531 – 5311PhosphoserineCombined sources
Cross-linki647 – 647Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei799 – 7991PhosphoserineBy similarity
Modified residuei1190 – 11901PhosphothreonineBy similarity
Modified residuei1192 – 11921PhosphoserineCombined sources
Cross-linki1246 – 1246Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Monoubiquitinated by the BCR(KLHL12) E3 ubiquitin ligase complex, leading to regulate the size of COPII coats.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9Z2Q1.
PRIDEiQ9Z2Q1.

PTM databases

iPTMnetiQ9Z2Q1.
PhosphoSiteiQ9Z2Q1.

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Gene expression databases

BgeeiENSRNOG00000002251.
ExpressionAtlasiQ9Z2Q1. baseline.
GenevisibleiQ9Z2Q1. RN.

Interactioni

Subunit structurei

Interacts with PDCD6 in a calcium-dependent manner. Interacts with KLHL12. SEC13 and SEC31 make a 2:2 tetramer that forms the edge element of the COPII outer coat. The tetramer self-assembles in multiple copies to form the complete polyhedral cage. Interacts (via WD 8) with SEC13 (By similarity). COPII is composed of at least 5 proteins: the SEC23/24 complex, the SEC13/31 complex and SAR1.By similarity2 Publications

Protein-protein interaction databases

BioGridi250159. 1 interaction.
IntActiQ9Z2Q1. 2 interactions.
STRINGi10116.ENSRNOP00000003072.

Structurei

3D structure databases

ProteinModelPortaliQ9Z2Q1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati4 – 4744WD 1Add
BLAST
Repeati64 – 11148WD 2Add
BLAST
Repeati120 – 16041WD 3Add
BLAST
Repeati166 – 20641WD 4Add
BLAST
Repeati209 – 25446WD 5Add
BLAST
Repeati258 – 29841WD 6Add
BLAST
Repeati301 – 34242WD 7Add
BLAST
Repeati397 – 42933WD 8; interaction with SEC13PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni161 – 470310Interaction with SEC13By similarityAdd
BLAST
Regioni800 – 1142343Interaction with PDCD6By similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi808 – 1120313Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the WD repeat SEC31 family.Curated
Contains 8 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0307. Eukaryota.
ENOG410XQ1D. LUCA.
GeneTreeiENSGT00390000003175.
HOGENOMiHOG000230582.
HOVERGENiHBG055626.
InParanoidiQ9Z2Q1.
KOiK14005.
PhylomeDBiQ9Z2Q1.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR024298. ACE1_Sec16_Sec31.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12931. Sec16_C. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Z2Q1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKLKEIDRTA MQAWSPAQNH PIYLATGTSA QQLDATFSTN ASLEIFELDL
60 70 80 90 100
SDPSLDMKSC ATFSSSHRYH KLIWGPHKMD SKGDVSGVLI AGGENGNIIL
110 120 130 140 150
YDPSKIIAGD KEVVIAQKDK HTGPVRALDV NIFQTNLVAS GANESEIYIW
160 170 180 190 200
DLNNFATPMT PGAKTQPPED ISCIAWNRQV QHILASASPS GRATVWDLRK
210 220 230 240 250
NEPIIKVSDH SNRMHCSGLA WHPDVATQMV LASEDDRLPV VQMWDLRFAS
260 270 280 290 300
SPLRVLENHA RGILAIAWSM ADPELLLSCG KDAKILCSNP NTGEVLYELP
310 320 330 340 350
TNTQWCFDIQ WCPRNPAVLS AASFDGRIRV YSIMGGSIDG LRQKQVDKLS
360 370 380 390 400
SSFGNLDPFG TGQPLPPLQI PQQTSQHSIV LPLKKPPKWI RRPVGASFSF
410 420 430 440 450
GGKLVTFENV TGQPQQGAEQ PRRQPVFISQ VVTEKDFLSR SEQLQHVVQS
460 470 480 490 500
QGFISYCQKK IDASQTDFEK NVWSFLKVNF EEDSRGKYLE LLGYRREDLG
510 520 530 540 550
EKIALALNRV DGSDVALKDS DRVAQSDGEE SPAEEGQLLG ERIKEEKQEC
560 570 580 590 600
DFLPSAGGGT FNISVSGDID GLITRALLTG NFESAVDLCL HDNRMADAII
610 620 630 640 650
LAIAGGQELL AQTQKKYFAK SQSKITRLIT AVVMKNWKEI VESCDLKNWR
660 670 680 690 700
EALAAVLTYA KPDEFSALCD LLGARLESEG DSLLRTQACL CYICAGNVER
710 720 730 740 750
LVACWTKAQD GSNPLSLQDL IEKVVILRKA VQLTQALDTN TVGALLAEKM
760 770 780 790 800
SQYANLLAAQ GSIAAALAFL PDNTNQPDIV QLRDRLCRAQ GRSVPGQESS
810 820 830 840 850
RSSYEGQPLP KGGPGPLAGH PQVSRVQSQQ YYPQVRIAPT VTTWSDRTPT
860 870 880 890 900
ALPSHPPAAC PSDTQGGNPP PPGFIMHGNV VPNSPAPLPT SPGHMHSQPP
910 920 930 940 950
PYPQPQPYQP AQQYSLGTGG SAVYRPQQPV APPASKRYPN APYVSPVASY
960 970 980 990 1000
SGQPHMYTAQ PASSPTSSSA PLPPPPSSGA SFQHGGPGAP PSSSAYALPP
1010 1020 1030 1040 1050
GTTGTPPAAS ELPASQRTGP QNGWNDPPAL NRVPKKKKLP ENFMPPVPIT
1060 1070 1080 1090 1100
SPIMNPGGDP QPQGLQQQPS ASGPRSSHAS FPQPHLAGGQ PFHGIQQPLA
1110 1120 1130 1140 1150
QTGMPPSFSK PNTEGAPGAP IGNTIQHVQA LPTEKITKKP IPDEHLILKT
1160 1170 1180 1190 1200
TFEDLIQRCL SSATDPQTKR KLDDASKRLE CLYDKLRDQT LSPTIISGLH
1210 1220 1230 1240
SIARSIETRN YSEGLTVHTH IVSTSNFSET SAFMPVLKVV LSQASKLGV
Length:1,249
Mass (Da):135,272
Last modified:July 10, 2007 - v2
Checksum:i8069E577903231B5
GO
Isoform 2 (identifier: Q9Z2Q1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     503-541: Missing.
     834-864: Missing.
     907-1019: PYQPAQQYSL...SELPASQRTG → R

Note: No experimental confirmation available.
Show »
Length:1,067
Mass (Da):116,879
Checksum:iFCC63F7487497926
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti209 – 2091D → N in AAD01990 (PubMed:10574704).Curated
Sequence conflicti213 – 2131R → W in AAD01990 (PubMed:10574704).Curated
Sequence conflicti449 – 4491Q → E in AAD01990 (PubMed:10574704).Curated
Sequence conflicti479 – 4791N → K in AAD01990 (PubMed:10574704).Curated
Sequence conflicti817 – 8171L → F in AAD01990 (PubMed:10574704).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei503 – 54139Missing in isoform 2. 1 PublicationVSP_026754Add
BLAST
Alternative sequencei834 – 86431Missing in isoform 2. 1 PublicationVSP_026755Add
BLAST
Alternative sequencei907 – 1019113PYQPA…SQRTG → R in isoform 2. 1 PublicationVSP_026756Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF034582 mRNA. Translation: AAD01990.1.
BC085722 mRNA. Translation: AAH85722.1.
PIRiT14150.
RefSeqiNP_148981.1. NM_033021.1.
XP_006250751.1. XM_006250689.2. [Q9Z2Q1-2]
XP_006250752.1. XM_006250690.1. [Q9Z2Q1-2]
UniGeneiRn.6322.

Genome annotation databases

EnsembliENSRNOT00000033489; ENSRNOP00000038196; ENSRNOG00000002251. [Q9Z2Q1-2]
GeneIDi93646.
KEGGirno:93646.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF034582 mRNA. Translation: AAD01990.1.
BC085722 mRNA. Translation: AAH85722.1.
PIRiT14150.
RefSeqiNP_148981.1. NM_033021.1.
XP_006250751.1. XM_006250689.2. [Q9Z2Q1-2]
XP_006250752.1. XM_006250690.1. [Q9Z2Q1-2]
UniGeneiRn.6322.

3D structure databases

ProteinModelPortaliQ9Z2Q1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi250159. 1 interaction.
IntActiQ9Z2Q1. 2 interactions.
STRINGi10116.ENSRNOP00000003072.

PTM databases

iPTMnetiQ9Z2Q1.
PhosphoSiteiQ9Z2Q1.

Proteomic databases

PaxDbiQ9Z2Q1.
PRIDEiQ9Z2Q1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000033489; ENSRNOP00000038196; ENSRNOG00000002251. [Q9Z2Q1-2]
GeneIDi93646.
KEGGirno:93646.

Organism-specific databases

CTDi22872.
RGDi620233. Sec31a.

Phylogenomic databases

eggNOGiKOG0307. Eukaryota.
ENOG410XQ1D. LUCA.
GeneTreeiENSGT00390000003175.
HOGENOMiHOG000230582.
HOVERGENiHBG055626.
InParanoidiQ9Z2Q1.
KOiK14005.
PhylomeDBiQ9Z2Q1.

Enzyme and pathway databases

ReactomeiR-RNO-204005. COPII (Coat Protein 2) Mediated Vesicle Transport.
R-RNO-2132295. MHC class II antigen presentation.
R-RNO-983170. Antigen Presentation: Folding, assembly and peptide loading of class I MHC.

Miscellaneous databases

PROiQ9Z2Q1.

Gene expression databases

BgeeiENSRNOG00000002251.
ExpressionAtlasiQ9Z2Q1. baseline.
GenevisibleiQ9Z2Q1. RN.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR024298. ACE1_Sec16_Sec31.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF12931. Sec16_C. 1 hit.
[Graphical view]
SMARTiSM00320. WD40. 6 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS50082. WD_REPEATS_2. 1 hit.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSC31A_RAT
AccessioniPrimary (citable) accession number: Q9Z2Q1
Secondary accession number(s): Q5RKK4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 10, 2007
Last sequence update: July 10, 2007
Last modified: September 7, 2016
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.