Reviewed, UniProtKB/Swiss-Prot Q9Z2L6 (MINP1_MOUSE)

Names and origin

Protein names

Recommended name:
    Multiple inositol polyphosphate phosphatase 1
    EC=3.1.3.62
Alternative name(s):
    Inositol (1,3,4,5)-tetrakisphosphate 3-phosphatase
    Ins(1,3,4,5)P(4) 3-phosphatase

Gene names

Name:	Minpp1
Synonyms:	Mipp
ORF Names:	MNCb-1572

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	481 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts as a phosphoinositide 5- and phosphoinositide 6-phosphatase and regulates cellular levels of inositol pentakisphosphate (InsP5) and inositol hexakisphosphate (InsP6). May play a role in bone development (endochondral ossification). Ref.4
Catalytic activity	Myo-inositol hexakisphosphate + H2O = myo-inositol pentakisphosphate (mixed isomers) + phosphate. Ref.1
Subcellular location	Endoplasmic reticulum lumen By similarity. Cell membrane Probable. Note: Also attached to the plasma membrane in erythrocytes Probable.
Tissue specificity	Widely expressed with highest levels in kidney, intestine, thymus and liver.
Developmental stage	Expressed in different stages of embryogenesis, from E7.5 through E14.5. The highest levels of expression is found in the visceral endoderm at E7.5 and E8.5 and in the fetal liver at E12.5 and E14.5. Ref.4
Sequence caution	The sequence BAA95058.1 differs from that shown. Reason: Frameshift at positions 14 and 176.

Ontologies

Keywords
Cellular component	Cell membrane Endoplasmic reticulum Membrane
Domain	Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW endoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acid phosphatase activity Inferred from electronic annotation. Source: InterPro multiple inositol-polyphosphate phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 30	30	Potential
	Chain	31 – 481	451	Multiple inositol polyphosphate phosphatase 1		PRO_0000019583
Regions
	Motif	478 – 481	4	Prevents secretion from ER Potential
Sites
	Active site	89	1	Potential
Amino acid modifications
	Glycosylation	236	1	N-linked (GlcNAc...) Potential
	Glycosylation	475	1	N-linked (GlcNAc...) Potential
Experimental info
	Mutagenesis	89	1	H → A: Loss of phosphatase activity. Ref.1
	Sequence conflict	211	1	D → G in AAD02434. Ref.1
	Sequence conflict	211	1	D → G in BAA95058. Ref.2
	Sequence conflict	257	1	K → Q in AAH21437. Ref.3
	Sequence conflict	267	1	V → M in AAD02434. Ref.1
	Sequence conflict	267	1	V → M in BAA95058. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Q9Z2L6-1 [UniParc]. Last modified July 5, 2005. Version 2. Checksum: A77C63B509FEFCF3	FASTA	481	54,563
	10 20 30 40 50 60 MLRGARSHLP ASVAPAAVLA AALLSSFARC SLPGRGDPVA SVLSPYFGTK TRYEDANPWL 70 80 90 100 110 120 LVDPVAPRRD PELLAGTCTP VQLVALIRHG TRYPTTKQIR KLKQLQGLLQ TRESRDGGSQ 130 140 150 160 170 180 VAAALAEWPL WYGDWMDGQL VEKGRQDMRQ LALRLAALFP DLFSRENYDR LRLITSSKHR 190 200 210 220 230 240 CVDSSAAFLQ GLWQHYHPGL PPPDVSDMEC DPPRINDKLM RFFDHCEKFL TDVERNETAL 250 260 270 280 290 300 YHVEAFKTGP EMQKVLKKVA ATLQVPVNSL NADLIQVAFF TCSFDLAIKG VHSPWCDVFD 310 320 330 340 350 360 VDDARVLEYL NDLKQYWKRS YGYTINSRSS CNLFQDIFLH LDKAVEQKQR SQPVSSPVIL 370 380 390 400 410 420 QFGHAETLLP LLSLMGYFKD KEPLTAYNFE EQVNRKFRSG HIVPYASNLI FVLYHCDNAQ 430 440 450 460 470 480 SPEEQFQIQL LLNEKVLPLA HSQRPVGLYE ELKTHYRDIL QSCQTSKECS PPKANITSDE L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Multiple inositol polyphosphate phosphatase: evolution as a distinct group within the histidine phosphatase family and chromosomal localization of the human and mouse genes to chromosomes 10q23 and 19." Chi H., Tiller G.E., Dasouki M.J., Romano P.R., Wang J., O'keefe R.J., Puzas J.E., Rosier R.N., Reynolds P.R. Genomics 56:324-336(1999) [PubMed: 10087200] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, MUTAGENESIS OF HIS-89. Strain: 129.
[2]	"Isolation of full-length cDNA clones from mouse brain cDNA library made by oligo-capping method." Osada N., Kusuda J., Tanuma R., Ito A., Hirata M., Sugano S., Hashimoto K. Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6. Tissue: Brain.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Czech II. Tissue: Mammary gland.
[4]	"Targeted deletion of Minpp1 provides new insight into the activity of multiple inositol polyphosphate phosphatase in vivo." Chi H., Yang X., Kingsley P.D., O'Keefe R.J., Puzas J.E., Rosier R.N., Shears S.B., Reynolds P.R. Mol. Cell. Biol. 20:6496-6507(2000) [PubMed: 10938126] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF046908 mRNA. Translation: AAD02434.1. AB041574 mRNA. Translation: BAA95058.1. Frameshift. BC021437 mRNA. Translation: AAH21437.1.
IPI	IPI00466733.
UniGene	Mm.255116
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000024896. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:1336159. Minpp1.
Phylogenomic databases
HOGENOM	Q9Z2L6.
HOVERGEN	Q9Z2L6.
Enzyme and pathway databases
BRENDA	3.1.3.62. 244.
Gene expression databases
ArrayExpress	Q9Z2L6.
Bgee	Q9Z2L6.
GermOnline	ENSMUSG00000024896. Mus musculus.
Family and domain databases
InterPro	IPR000886. ER_targeting_sequence. IPR000560. Histidine_acid_Pase. IPR016274. Histidine_acid_Pase_euk. [Graphical view]
Pfam	PF00328. Acid_phosphat_A. 1 hit. [Graphical view]
PIRSF	PIRSF000894. Acid_phosphatase. 1 hit.
PROSITE	PS00014. ER_TARGET. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

MINP1_MOUSE

Accession

Primary (citable) accession number: Q9Z2L6
Secondary accession number(s): Q8VDR0, Q9JJD5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2005
Last sequence update:	July 5, 2005
Last modified:	June 16, 2009
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)