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Protein

Voltage-dependent anion-selective channel protein 1

Gene

Vdac1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective (PubMed:25628567). May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis (By similarity).By similarity1 Publication

Enzyme regulationi

Inhibited by nitric oxide.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei73 – 731Involved in hexokinase bindingBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi242 – 2443NADBy similarity
Nucleotide bindingi260 – 2645NADBy similarity

GO - Molecular functioni

  • anion channel activity Source: RGD
  • nucleotide binding Source: UniProtKB-KW
  • porin activity Source: UniProtKB-KW
  • protein complex binding Source: RGD
  • voltage-gated anion channel activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Porin

Keywords - Biological processi

Apoptosis, Ion transport, Transport

Keywords - Ligandi

NAD, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-5205685. Pink/Parkin Mediated Mitophagy.

Names & Taxonomyi

Protein namesi
Recommended name:
Voltage-dependent anion-selective channel protein 1
Short name:
VDAC-1
Short name:
rVDAC1
Alternative name(s):
Outer mitochondrial membrane protein porin 1
Gene namesi
Name:Vdac1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi621575. Vdac1.

Subcellular locationi

  • Mitochondrion outer membrane By similarity; Multi-pass membrane protein By similarity
  • Cell membrane By similarity; Multi-pass membrane protein By similarity
  • Membrane raft By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei26 – 3510Beta strandedBy similarity
Transmembranei39 – 479Beta strandedBy similarity
Transmembranei54 – 6411Beta strandedBy similarityAdd
BLAST
Transmembranei69 – 768Beta strandedBy similarity
Transmembranei80 – 8910Beta strandedBy similarity
Transmembranei95 – 10410Beta strandedBy similarity
Transmembranei111 – 12010Beta strandedBy similarity
Transmembranei123 – 1308Beta strandedBy similarity
Transmembranei137 – 1459Beta strandedBy similarity
Transmembranei150 – 1589Beta strandedBy similarity
Transmembranei163 – 17513Beta strandedBy similarityAdd
BLAST
Transmembranei178 – 1858Beta strandedBy similarity
Transmembranei189 – 19810Beta strandedBy similarity
Transmembranei202 – 21110Beta strandedBy similarity
Transmembranei218 – 22710Beta strandedBy similarity
Transmembranei231 – 2388Beta strandedBy similarity
Transmembranei242 – 25110Beta strandedBy similarity
Transmembranei254 – 26310Beta strandedBy similarity
Transmembranei273 – 28210Beta strandedBy similarity

GO - Cellular componenti

  • extracellular exosome Source: Ensembl
  • membrane Source: UniProtKB
  • membrane raft Source: UniProtKB-SubCell
  • mitochondrial inner membrane Source: Ensembl
  • mitochondrial nucleoid Source: Ensembl
  • mitochondrial outer membrane Source: RGD
  • mitochondrion Source: RGD
  • myelin sheath Source: Ensembl
  • nucleus Source: Ensembl
  • plasma membrane Source: UniProtKB
  • pore complex Source: UniProtKB-KW
  • protein complex Source: RGD
  • synaptic vesicle Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi137 – 1371S → E: Channel conformation is shifted toward the open state. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 283282Voltage-dependent anion-selective channel protein 1PRO_0000050503Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication
Modified residuei13 – 131Phosphoserine1 Publication
Modified residuei19 – 191PhosphothreonineCombined sources
Modified residuei20 – 201N6-acetyllysine; alternateBy similarity
Modified residuei20 – 201N6-succinyllysine; alternateBy similarity
Cross-linki53 – 53Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei67 – 671PhosphotyrosineBy similarity
Modified residuei107 – 1071PhosphothreonineBy similarity
Modified residuei109 – 1091N6-acetyllysine; alternateBy similarity
Cross-linki109 – 109Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Cross-linki110 – 110Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei137 – 1371Phosphoserine1 Publication
Cross-linki161 – 161Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei193 – 1931Phosphoserine; by NEK1By similarity
Modified residuei240 – 2401PhosphoserineBy similarity
Modified residuei252 – 2521N6-acetyllysineBy similarity
Modified residuei266 – 2661N6-acetyllysine; alternateBy similarity
Cross-linki266 – 266Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity

Post-translational modificationi

Phosphorylation at Ser-193 by NEK1 promotes the open conformational state preventing excessive mitochondrial membrane permeability and subsequent apoptotic cell death after injury. Phosphorylation by the AKT-GSK3B axis stabilizes the protein probably by preventing ubiquitin-mediated proteasomal degradation.By similarity
Ubiquitinated by PARK2 during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9Z2L0.
PRIDEiQ9Z2L0.

2D gel databases

World-2DPAGE0004:Q9Z2L0.

PTM databases

iPTMnetiQ9Z2L0.
PhosphoSiteiQ9Z2L0.
SwissPalmiQ9Z2L0.

Expressioni

Tissue specificityi

Widely expressed. High levels in heart and kidney with lower levels in brain and ascitic tumor. Very low levels in liver.1 Publication

Gene expression databases

GenevisibleiQ9Z2L0. RN.

Interactioni

Subunit structurei

Interacts with hexokinases including HK1. The HK1-VDAC1 complex interacts with ATF2. Interacts with BCL2L1. Interacts with BAK1. Interacts with BOP/C22orf29 (via BH3 domain). Interacts with beta amyloid and APP; induces VDAC1 dephosphorylation.By similarity

GO - Molecular functioni

  • protein complex binding Source: RGD

Protein-protein interaction databases

BioGridi249746. 3 interactions.
DIPiDIP-37068N.
IntActiQ9Z2L0. 5 interactions.
MINTiMINT-1541752.
STRINGi10116.ENSRNOP00000008477.

Family & Domainsi

Domaini

Consists mainly of a membrane-spanning beta-barrel formed by 19 beta-strands. The helical N-terminus folds back into the pore opening and plays a role in voltage-gated channel activity (By similarity).By similarity

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiKOG3126. Eukaryota.
ENOG410ZBK1. LUCA.
GeneTreeiENSGT00390000011336.
HOVERGENiHBG054036.
InParanoidiQ9Z2L0.
KOiK05862.
OMAiYGLMFTE.
OrthoDBiEOG7P8P8G.
PhylomeDBiQ9Z2L0.
TreeFamiTF315091.

Family and domain databases

Gene3Di2.40.160.10. 1 hit.
InterProiIPR023614. Porin_dom.
IPR001925. Porin_Euk.
IPR027246. Porin_Euk/Tom40.
IPR030270. VDAC1.
[Graphical view]
PANTHERiPTHR11743:SF13. PTHR11743:SF13. 1 hit.
PfamiPF01459. Porin_3. 1 hit.
[Graphical view]
PRINTSiPR00185. EUKARYTPORIN.
PROSITEiPS00558. EUKARYOTIC_PORIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z2L0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVPPTYADL GKSARDVFTK GYGFGLIKLD LKTKSENGLE FTSSGSANTE
60 70 80 90 100
TTKVNGSLET KYRWTEYGLT FTEKWNTDNT LGTEITVEDQ LARGLKLTFD
110 120 130 140 150
SSFSPNTGKK NAKIKTGYKR EHINLGCDVD FDIAGPSIRG ALVLGYEGWL
160 170 180 190 200
AGYQMNFETS KSRVTQSNFA VGYKTDEFQL HTNVNDGTEF GGSIYQKVNK
210 220 230 240 250
KLETAVNLAW TAGNSNTRFG IAAKYQVDPD ACFSAKVNNS SLIGLGYTQT
260 270 280
LKPGIKLTLS ALLDGKNVNA GGHKLGLGLE FQA
Length:283
Mass (Da):30,756
Last modified:January 23, 2007 - v4
Checksum:iD32B05B8A8D75732
GO

Sequence cautioni

The sequence AAH60558.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti55 – 551N → T in AAD02476 (PubMed:9714728).Curated
Sequence conflicti83 – 831T → S in AAD02476 (PubMed:9714728).Curated
Sequence conflicti129 – 1291V → M in AAD02476 (PubMed:9714728).Curated
Sequence conflicti148 – 1481G → GFDIAGPSIRGALVLGYEG in AAD02476 (PubMed:9714728).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF048828 mRNA. Translation: AAD02476.1.
AB039662 mRNA. Translation: BAB13473.1.
AF268467 mRNA. Translation: AAF80115.1.
BC060558 mRNA. Translation: AAH60558.1. Different initiation.
BC072484 mRNA. Translation: AAH72484.2.
BC087573 mRNA. Translation: AAH87573.2.
BC087657 mRNA. Translation: AAH87657.2.
BC104684 mRNA. Translation: AAI04685.2.
BC127491 mRNA. Translation: AAI27492.1.
RefSeqiNP_112643.1. NM_031353.1.
UniGeneiRn.54594.

Genome annotation databases

EnsembliENSRNOT00000008477; ENSRNOP00000008477; ENSRNOG00000006375.
GeneIDi83529.
KEGGirno:83529.
UCSCiRGD:621575. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF048828 mRNA. Translation: AAD02476.1.
AB039662 mRNA. Translation: BAB13473.1.
AF268467 mRNA. Translation: AAF80115.1.
BC060558 mRNA. Translation: AAH60558.1. Different initiation.
BC072484 mRNA. Translation: AAH72484.2.
BC087573 mRNA. Translation: AAH87573.2.
BC087657 mRNA. Translation: AAH87657.2.
BC104684 mRNA. Translation: AAI04685.2.
BC127491 mRNA. Translation: AAI27492.1.
RefSeqiNP_112643.1. NM_031353.1.
UniGeneiRn.54594.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249746. 3 interactions.
DIPiDIP-37068N.
IntActiQ9Z2L0. 5 interactions.
MINTiMINT-1541752.
STRINGi10116.ENSRNOP00000008477.

PTM databases

iPTMnetiQ9Z2L0.
PhosphoSiteiQ9Z2L0.
SwissPalmiQ9Z2L0.

2D gel databases

World-2DPAGE0004:Q9Z2L0.

Proteomic databases

PaxDbiQ9Z2L0.
PRIDEiQ9Z2L0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000008477; ENSRNOP00000008477; ENSRNOG00000006375.
GeneIDi83529.
KEGGirno:83529.
UCSCiRGD:621575. rat.

Organism-specific databases

CTDi7416.
RGDi621575. Vdac1.

Phylogenomic databases

eggNOGiKOG3126. Eukaryota.
ENOG410ZBK1. LUCA.
GeneTreeiENSGT00390000011336.
HOVERGENiHBG054036.
InParanoidiQ9Z2L0.
KOiK05862.
OMAiYGLMFTE.
OrthoDBiEOG7P8P8G.
PhylomeDBiQ9Z2L0.
TreeFamiTF315091.

Enzyme and pathway databases

ReactomeiR-RNO-5205685. Pink/Parkin Mediated Mitophagy.

Miscellaneous databases

PROiQ9Z2L0.

Gene expression databases

GenevisibleiQ9Z2L0. RN.

Family and domain databases

Gene3Di2.40.160.10. 1 hit.
InterProiIPR023614. Porin_dom.
IPR001925. Porin_Euk.
IPR027246. Porin_Euk/Tom40.
IPR030270. VDAC1.
[Graphical view]
PANTHERiPTHR11743:SF13. PTHR11743:SF13. 1 hit.
PfamiPF01459. Porin_3. 1 hit.
[Graphical view]
PRINTSiPR00185. EUKARYTPORIN.
PROSITEiPS00558. EUKARYOTIC_PORIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of rat porin isoforms: cloning of a cardiac type-3 variant encoding an additional methionine at its putative N-terminal region."
    Anflous K., Blondel O., Bernard A., Khrestchatisky M., Ventura-Clapier R.
    Biochim. Biophys. Acta 1399:47-50(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Heart.
  2. "Characterization of porin isoforms expressed in tumor cells."
    Shinohara Y., Ishida T., Hino M., Yamazaki N., Baba Y., Terada H.
    Eur. J. Biochem. 267:6067-6073(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Ascitic tumor.
  3. "Ion channels in the lens."
    Rae J.L.
    Submitted (MAY-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Lens.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain, Embryonic brain, Heart, Ovary, Pituitary and Placenta.
  5. "Post-translational modifications of rat liver mitochondrial outer membrane proteins identified by mass spectrometry."
    Distler A.M., Kerner J., Hoppel C.L.
    Biochim. Biophys. Acta 1774:628-636(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 2-14 AND 135-138, ACETYLATION AT ALA-2, PHOSPHORYLATION AT SER-13 AND SER-137, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Liver.
  6. Lubec G., Chen W.-Q., Afjehi-Sadat L., Kang S.U.
    Submitted (JUL-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 21-28; 33-53; 64-74; 75-93; 97-109; 121-139; 164-197; 201-218 AND 225-266, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain, Hippocampus and Spinal cord.
  7. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-19, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Markov chain Monte Carlo based analysis of post-translationally modified VDAC gating kinetics."
    Tewari S.G., Zhou Y., Otto B.J., Dash R.K., Kwok W.M., Beard D.A.
    Front. Physiol. 5:513-513(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF SER-137.

Entry informationi

Entry nameiVDAC1_RAT
AccessioniPrimary (citable) accession number: Q9Z2L0
Secondary accession number(s): A1L125
, Q3MHT8, Q5M944, Q5M972, Q6IN28, Q6P9W9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 117 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.