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Protein

Keratin, type I cytoskeletal 16

Gene

Krt16

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Epidermis-specific type I keratin that plays a key role in skin (PubMed:22336941, PubMed:24218583). Acts as a regulator of innate immunity in response to skin barrier breach: required for some inflammatory checkpoint for the skin barrier maintenance (PubMed:24218583).3 Publications

GO - Molecular functioni

  • structural constituent of cytoskeleton Source: MGI

GO - Biological processi

  • aging Source: MGI
  • establishment of skin barrier Source: UniProtKB
  • hair cycle Source: MGI
  • inflammatory response Source: UniProtKB
  • innate immune response Source: UniProtKB
  • intermediate filament cytoskeleton organization Source: MGI
  • keratinization Source: UniProtKB
  • keratinocyte differentiation Source: UniProtKB
  • keratinocyte migration Source: UniProtKB
  • morphogenesis of an epithelium Source: UniProtKB
  • negative regulation of cell migration Source: MGI
Complete GO annotation...

Keywords - Biological processi

Immunity, Innate immunity

Names & Taxonomyi

Protein namesi
Recommended name:
Keratin, type I cytoskeletal 16
Alternative name(s):
Cytokeratin-16
Short name:
CK-16
Keratin-16
Short name:
K16
Gene namesi
Name:Krt16
Synonyms:Krt1-16
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:96690. Krt16.

Subcellular locationi

GO - Cellular componenti

  • cytoskeleton Source: MGI
  • extracellular exosome Source: MGI
  • intermediate filament Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Intermediate filament, Keratin

Pathology & Biotechi

Disruption phenotypei

Mice were born alive at approximately Mendelian ratios but increased postnatal mortality is observed. Surviving mice show oral lesions as well as palmoplantar keratoderma-like hyperkeratotic calluses on front and hind paws, which impair the ability to walk.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 469469Keratin, type I cytoskeletal 16PRO_0000063663Add
BLAST

Proteomic databases

MaxQBiQ9Z2K1.
PaxDbiQ9Z2K1.
PRIDEiQ9Z2K1.

PTM databases

iPTMnetiQ9Z2K1.
PhosphoSiteiQ9Z2K1.

Expressioni

Tissue specificityi

Epidermis-specific. Expressed in stratified epithelia of glabrous skin, the oral mucosa and several appendages. In mature hair follicles, expressed in the companion layer of the outer root sheath during anagen and in the club hair sheath during catagen and telogen.1 Publication

Developmental stagei

During embryonic development, initially localizes within early hair germs, but rapidly shifts to a subset of cells at the interface of basal and suprabasal cells above and around the hair germ.1 Publication

Inductioni

In response to epidermal stress such as wounding.1 Publication

Gene expression databases

BgeeiQ9Z2K1.
CleanExiMM_KRT16.
ExpressionAtlasiQ9Z2K1. baseline and differential.
GenevisibleiQ9Z2K1. MM.

Interactioni

Subunit structurei

Heterodimer of a type I and a type II keratin. KRT16 associates with KRT6 isomers (KRT6A or KRT6B) (PubMed:8636216). Interacts with TCHP (By similarity). Interacts with TRADD (PubMed:16702408).By similarity2 Publications

Protein-protein interaction databases

BioGridi201021. 5 interactions.
IntActiQ9Z2K1. 2 interactions.
MINTiMINT-4099313.
STRINGi10090.ENSMUSP00000007280.

Structurei

3D structure databases

ProteinModelPortaliQ9Z2K1.
SMRiQ9Z2K1. Positions 163-261, 330-419.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 112112HeadAdd
BLAST
Regioni113 – 420308RodAdd
BLAST
Regioni113 – 14836Coil 1AAdd
BLAST
Regioni149 – 16618Linker 1Add
BLAST
Regioni167 – 25892Coil 1BAdd
BLAST
Regioni259 – 28123Linker 12Add
BLAST
Regioni282 – 420139Coil 2Add
BLAST
Regioni421 – 46949TailAdd
BLAST

Sequence similaritiesi

Belongs to the intermediate filament family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IFTF. Eukaryota.
ENOG410Y9IV. LUCA.
GeneTreeiENSGT00760000118808.
HOVERGENiHBG013015.
InParanoidiQ9Z2K1.
KOiK07604.
OMAiGHQTRPI.
OrthoDBiEOG7FV3Q8.
TreeFamiTF332742.

Family and domain databases

InterProiIPR001664. IF.
IPR018039. Intermediate_filament_CS.
IPR002957. Keratin_I.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PfamiPF00038. Filament. 1 hit.
[Graphical view]
PRINTSiPR01248. TYPE1KERATIN.
SMARTiSM01391. Filament. 1 hit.
[Graphical view]
PROSITEiPS00226. IF. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z2K1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATCSRQFTS SSSMKGSCGI GGGSSRMSSI LAGGSCRAPS TCGGMSVTSS
60 70 80 90 100
RFSSGGVCGI GGGYGGSFSS SSFGGGLGSG FGGRFDGFGG GFGAGLGGGL
110 120 130 140 150
GGGIGDGLLV GSEKVTMQNL NDRLATYLDK VRALEEANRD LEVKIRDWYQ
160 170 180 190 200
RQRPTEIKDY SPYFKTIEDL KSKIIIATQE NAQFTLQIDN ARLAADDFRT
210 220 230 240 250
KYENELFLRQ SVEGDINGLR KVLDELTLSR ADLEMQIENL REELAFLKKN
260 270 280 290 300
HEEEMLALRG QTGGDVNVEM DAAPGVDLSR ILNEMRDQYE QMAEKNRRDV
310 320 330 340 350
EAWFLRKTEE LNKEVASNSD LIQSNRSEVA ELRRVFQGLE IELQSQLSMK
360 370 380 390 400
ASLENSLEET KGRYCMQLSQ IQGLISSVEE QLAQLRCEME QQSQEYNILL
410 420 430 440 450
DVKTRLEQEI ATYRRLLDGE NIHSSSQHSS GQSYSSREVF SSSSRQPRSI
460
LKEQGSTSFS QSQSQSSRD
Length:469
Mass (Da):51,606
Last modified:January 23, 2007 - v3
Checksum:i13B8028CF28B64D5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053235 mRNA. Translation: AAC79424.1.
CCDSiCCDS25414.1.
RefSeqiNP_001300887.1. NM_001313958.1.
NP_032496.1. NM_008470.1.
UniGeneiMm.422799.

Genome annotation databases

EnsembliENSMUST00000007280; ENSMUSP00000007280; ENSMUSG00000053797.
GeneIDi16666.
KEGGimmu:16666.
UCSCiuc007lkq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053235 mRNA. Translation: AAC79424.1.
CCDSiCCDS25414.1.
RefSeqiNP_001300887.1. NM_001313958.1.
NP_032496.1. NM_008470.1.
UniGeneiMm.422799.

3D structure databases

ProteinModelPortaliQ9Z2K1.
SMRiQ9Z2K1. Positions 163-261, 330-419.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201021. 5 interactions.
IntActiQ9Z2K1. 2 interactions.
MINTiMINT-4099313.
STRINGi10090.ENSMUSP00000007280.

PTM databases

iPTMnetiQ9Z2K1.
PhosphoSiteiQ9Z2K1.

Proteomic databases

MaxQBiQ9Z2K1.
PaxDbiQ9Z2K1.
PRIDEiQ9Z2K1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000007280; ENSMUSP00000007280; ENSMUSG00000053797.
GeneIDi16666.
KEGGimmu:16666.
UCSCiuc007lkq.2. mouse.

Organism-specific databases

CTDi3868.
MGIiMGI:96690. Krt16.

Phylogenomic databases

eggNOGiENOG410IFTF. Eukaryota.
ENOG410Y9IV. LUCA.
GeneTreeiENSGT00760000118808.
HOVERGENiHBG013015.
InParanoidiQ9Z2K1.
KOiK07604.
OMAiGHQTRPI.
OrthoDBiEOG7FV3Q8.
TreeFamiTF332742.

Miscellaneous databases

PROiQ9Z2K1.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Z2K1.
CleanExiMM_KRT16.
ExpressionAtlasiQ9Z2K1. baseline and differential.
GenevisibleiQ9Z2K1. MM.

Family and domain databases

InterProiIPR001664. IF.
IPR018039. Intermediate_filament_CS.
IPR002957. Keratin_I.
[Graphical view]
PANTHERiPTHR23239. PTHR23239. 1 hit.
PfamiPF00038. Filament. 1 hit.
[Graphical view]
PRINTSiPR01248. TYPE1KERATIN.
SMARTiSM01391. Filament. 1 hit.
[Graphical view]
PROSITEiPS00226. IF. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "cDNA cloning, expression, and assembly characteristics of mouse keratin 16."
    Porter R.M., Hutcheson A.M., Rugg E.L., Quinlan R.A., Lane E.B.
    J. Biol. Chem. 273:32265-32272(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
  2. "Onset of re-epithelialization after skin injury correlates with a reorganization of keratin filaments in wound edge keratinocytes: defining a potential role for keratin 16."
    Paladini R.D., Takahashi K., Bravo N.S., Coulombe P.A.
    J. Cell Biol. 132:381-397(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION, SUBUNIT.
  3. "Forced expression of keratin 16 alters the adhesion, differentiation, and migration of mouse skin keratinocytes."
    Wawersik M., Coulombe P.A.
    Mol. Biol. Cell 11:3315-3327(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Keratin 16 expression defines a subset of epithelial cells during skin morphogenesis and the hair cycle."
    Bernot K.M., Coulombe P.A., McGowan K.M.
    J. Invest. Dermatol. 119:1137-1149(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  5. "Keratin 17 modulates hair follicle cycling in a TNFalpha-dependent fashion."
    Tong X., Coulombe P.A.
    Genes Dev. 20:1353-1364(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH TRADD.
  6. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  7. "Keratin 16-null mice develop palmoplantar keratoderma, a hallmark feature of pachyonychia congenita and related disorders."
    Lessard J.C., Coulombe P.A.
    J. Invest. Dermatol. 132:1384-1391(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE.
  8. Cited for: FUNCTION.

Entry informationi

Entry nameiK1C16_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2K1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 112 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.