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Protein

Prostaglandin D2 receptor 2

Gene

Ptgdr2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor for prostaglandin D2 (PGD2). Coupled to the G(i)-protein. Receptor activation may result in pertussis toxin-sensitive decreases in cAMP levels and Ca2+ mobilization. PI3K signaling is also implicated in mediating PTGDR2 effects. PGD2 induced receptor internalization. CRTH2 internalization can be regulated by diverse kinases such as, PKC, PKA, ADRBK1/GRK2, GPRK5/GRK5 and GRK6. Receptor activation is responsible, at least in part, in immune regulation and allergic/inflammation responses (By similarity).By similarity2 Publications

GO - Molecular functioni

  • G-protein coupled receptor activity Source: MGI
  • neuropeptide binding Source: GO_Central
  • prostaglandin D receptor activity Source: MGI
  • prostaglandin F receptor activity Source: MGI
  • prostaglandin J receptor activity Source: MGI

GO - Biological processi

  • adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Source: MGI
  • calcium-mediated signaling Source: MGI
  • chemotaxis Source: MGI
  • G-protein coupled receptor signaling pathway Source: MGI
  • negative regulation of male germ cell proliferation Source: MGI
  • neuropeptide signaling pathway Source: GO_Central
  • positive regulation of G-protein coupled receptor protein signaling pathway Source: MGI
  • synaptic transmission Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

G-protein coupled receptor, Receptor, Transducer

Enzyme and pathway databases

ReactomeiR-MMU-391908. Prostanoid ligand receptors.
R-MMU-418594. G alpha (i) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Prostaglandin D2 receptor 2
Alternative name(s):
Chemoattractant receptor-homologous molecule expressed on Th2 cells
G-protein coupled receptor 44
CD_antigen: CD294, Gpr44
Gene namesi
Name:Ptgdr2
Synonyms:Crth2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1330275. Ptgdr2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3232ExtracellularSequence analysisAdd
BLAST
Transmembranei33 – 5523Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini56 – 6611CytoplasmicSequence analysisAdd
BLAST
Transmembranei67 – 8822Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini89 – 10517ExtracellularSequence analysisAdd
BLAST
Transmembranei106 – 12621Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini127 – 14519CytoplasmicSequence analysisAdd
BLAST
Transmembranei146 – 16722Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini168 – 20942ExtracellularSequence analysisAdd
BLAST
Transmembranei210 – 23021Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini231 – 24616CytoplasmicSequence analysisAdd
BLAST
Transmembranei247 – 26822Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini269 – 28719ExtracellularSequence analysisAdd
BLAST
Transmembranei288 – 30720Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini308 – 35750CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Deficient mice are fertile and grew normally. Ear-swelling responses induced by hapten-specific IgE are less pronounced in deficient mice, giving 35-55% of the responses of normal mice. The reduction in cutaneous responses is associated with decreased infiltration of lymphocytes, eosinophils, and basophils and decreased production of macrophage-derived chemokine and RANTES at inflammatory sites. In models of chronic contact hypersensitivity induced by repeated hapten application, CRTH2-deficient mice result in a reduction by approximately half of skin responses and low levels (63% of control) of serum IgE production.1 Publication

Chemistry

ChEMBLiCHEMBL2291.
GuidetoPHARMACOLOGYi339.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 382382Prostaglandin D2 receptor 2PRO_0000069573Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi3 – 31N-linked (GlcNAc...)Sequence analysis
Glycosylationi21 – 211N-linked (GlcNAc...)Sequence analysis
Glycosylationi24 – 241N-linked (GlcNAc...)Sequence analysis
Disulfide bondi103 ↔ 181PROSITE-ProRule annotation
Modified residuei330 – 3301PhosphoserineBy similarity
Modified residuei344 – 3441PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9Z2J6.
PRIDEiQ9Z2J6.

PTM databases

iPTMnetiQ9Z2J6.
PhosphoSiteiQ9Z2J6.

Expressioni

Gene expression databases

BgeeiQ9Z2J6.
CleanExiMM_GPR44.
GenevisibleiQ9Z2J6. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036159.

Chemistry

BindingDBiQ9Z2J6.

Structurei

3D structure databases

ProteinModelPortaliQ9Z2J6.
SMRiQ9Z2J6. Positions 37-305.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi329 – 3324Involved in the recycling of CRTH2By similarity

Domaini

The 329-DSEL-332 motif is involved in the recycling of PTGDR2 to the cell surface after agonist-induced internalization. This motif seems to be required for ADRBK1/GRK2 and GPRK5/GRK5 to promote agonist-induced internalization (By similarity). Thr-346 is a major site for PKC-induced internalization of the receptor (By similarity).By similarity

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IV6V. Eukaryota.
ENOG410YAX7. LUCA.
GeneTreeiENSGT00760000118990.
HOGENOMiHOG000234122.
HOVERGENiHBG099857.
InParanoidiQ9Z2J6.
KOiK06715.
OMAiGCRMRQT.
OrthoDBiEOG71RXKP.
PhylomeDBiQ9Z2J6.
TreeFamiTF330976.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q9Z2J6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANVTLKPLC PLLEEMVQLP NHSNSSLRYI DHVSVLLHGL ASLLGLVENG
60 70 80 90 100
LILFVVGCRM RQTVVTTWVL HLALSDLLAA ASLPFFTYFL AVGHSWELGT
110 120 130 140 150
TFCKLHSSVF FLNMFASGFL LSAISLDRCL QVVRPVWAQN HRTVAVAHRV
160 170 180 190 200
CLMLWALAVL NTIPYFVFRD TIPRLDGRIM CYYNLLLWNP GPDRDTTCDY
210 220 230 240 250
RQKALAVSKF LLAFMVPLAI IASSHVAVSL RLHHRGRQRT GRFVRLVAAI
260 270 280 290 300
VVAFVLCWGP YHIFSLLEAR AHSVTTLRQL ASRGLPFVTS LAFFNSVVNP
310 320 330 340 350
LLYVFTCPDM LYKLRRSLRA VLESVLVEDS DQSGGLRNRR RRASSTATPA
360 370 380
STLLLADRIP QLRPTRLIGW MRRGSAEVPQ RV
Length:382
Mass (Da):42,950
Last modified:May 1, 1999 - v1
Checksum:i8CCB898B93795B04
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF054507 Genomic DNA. Translation: AAD13525.1.
AB109091 Genomic DNA. Translation: BAC81436.1.
AB109092 mRNA. Translation: BAC81437.1.
CH466534 Genomic DNA. Translation: EDL41395.1.
BC111906 mRNA. Translation: AAI11907.1.
CCDSiCCDS29592.1.
RefSeqiNP_034092.1. NM_009962.3.
XP_006526758.1. XM_006526695.2.
XP_006526759.1. XM_006526696.2.
XP_011245450.1. XM_011247148.1.
UniGeneiMm.470721.

Genome annotation databases

EnsembliENSMUST00000037261; ENSMUSP00000036159; ENSMUSG00000034117.
GeneIDi14764.
KEGGimmu:14764.
UCSCiuc008gri.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF054507 Genomic DNA. Translation: AAD13525.1.
AB109091 Genomic DNA. Translation: BAC81436.1.
AB109092 mRNA. Translation: BAC81437.1.
CH466534 Genomic DNA. Translation: EDL41395.1.
BC111906 mRNA. Translation: AAI11907.1.
CCDSiCCDS29592.1.
RefSeqiNP_034092.1. NM_009962.3.
XP_006526758.1. XM_006526695.2.
XP_006526759.1. XM_006526696.2.
XP_011245450.1. XM_011247148.1.
UniGeneiMm.470721.

3D structure databases

ProteinModelPortaliQ9Z2J6.
SMRiQ9Z2J6. Positions 37-305.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000036159.

Chemistry

BindingDBiQ9Z2J6.
ChEMBLiCHEMBL2291.
GuidetoPHARMACOLOGYi339.

Protein family/group databases

GPCRDBiSearch...

PTM databases

iPTMnetiQ9Z2J6.
PhosphoSiteiQ9Z2J6.

Proteomic databases

PaxDbiQ9Z2J6.
PRIDEiQ9Z2J6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037261; ENSMUSP00000036159; ENSMUSG00000034117.
GeneIDi14764.
KEGGimmu:14764.
UCSCiuc008gri.1. mouse.

Organism-specific databases

CTDi11251.
MGIiMGI:1330275. Ptgdr2.

Phylogenomic databases

eggNOGiENOG410IV6V. Eukaryota.
ENOG410YAX7. LUCA.
GeneTreeiENSGT00760000118990.
HOGENOMiHOG000234122.
HOVERGENiHBG099857.
InParanoidiQ9Z2J6.
KOiK06715.
OMAiGCRMRQT.
OrthoDBiEOG71RXKP.
PhylomeDBiQ9Z2J6.
TreeFamiTF330976.

Enzyme and pathway databases

ReactomeiR-MMU-391908. Prostanoid ligand receptors.
R-MMU-418594. G alpha (i) signalling events.

Miscellaneous databases

PROiQ9Z2J6.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Z2J6.
CleanExiMM_GPR44.
GenevisibleiQ9Z2J6. MM.

Family and domain databases

InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
[Graphical view]
PRINTSiPR00237. GPCRRHODOPSN.
PROSITEiPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning, chromosome mapping and characterization of the mouse CRTH2 gene, a putative member of the leukocyte chemoattractant receptor family."
    Abe H., Takeshita T., Nagata K., Arita T., Endo Y., Fujita T., Takayama H., Kubo M., Sugamura K.
    Gene 227:71-77(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJ.
  2. "Gene structure and functional properties of mouse CRTH2, a prostaglandin D2 receptor."
    Hirai H., Abe H., Tanaka K., Takatsu K., Sugamura K., Nakamura M., Nagata K.
    Biochem. Biophys. Res. Commun. 307:797-802(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION AS RECEPTOR FOR PGD2, CHARACTERIZATION.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  5. "Prostaglandin D2 plays an essential role in chronic allergic inflammation of the skin via CRTH2 receptor."
    Satoh T., Moroi R., Aritake K., Urade Y., Kanai Y., Sumi K., Yokozeki H., Hirai H., Nagata K., Hara T., Utsuyama M., Hirokawa K., Sugamura K., Nishioka K., Nakamura M.
    J. Immunol. 177:2621-2629(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-344, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Spleen.

Entry informationi

Entry nameiPD2R2_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2J6
Secondary accession number(s): Q54A00
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 1, 1999
Last modified: July 6, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.