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Q9Z2J4

- NEXN_RAT

UniProt

Q9Z2J4 - NEXN_RAT

Protein

Nexilin

Gene

Nexn

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 May 1999)
      Previous versions | rss
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    Functioni

    Involved in regulating cell migration through association with the actin cytoskeleton. Has an essential role in the maintenance of Z line and sarcomere integrity.By similarity

    GO - Molecular functioni

    1. actin filament binding Source: RGD
    2. calmodulin-dependent protein kinase activity Source: RefGenome
    3. structural constituent of muscle Source: UniProtKB

    GO - Biological processi

    1. cardiac muscle fiber development Source: RefGenome
    2. protein phosphorylation Source: GOC
    3. regulation of cell migration Source: UniProtKB
    4. regulation of cytoskeleton organization Source: UniProtKB

    Keywords - Ligandi

    Actin-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nexilin
    Gene namesi
    Name:NexnImported
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 2

    Organism-specific databases

    RGDi708354. Nexn.

    Subcellular locationi

    Cytoplasmcytoskeleton 1 Publication. Cell junctionadherens junction 1 Publication. CytoplasmmyofibrilsarcomereZ line
    Note: Localizes to the cell-matrix AJ. Not found at the cell-cell AJ.1 Publication

    GO - Cellular componenti

    1. cell-substrate adherens junction Source: UniProtKB
    2. cytoskeleton Source: UniProtKB-SubCell
    3. Z disc Source: UniProtKB

    Keywords - Cellular componenti

    Cell junction, Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 656656NexilinPRO_0000302087Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei80 – 801PhosphoserineBy similarity
    Modified residuei221 – 2211PhosphoserineBy similarity
    Modified residuei337 – 3371PhosphoserineBy similarity
    Modified residuei345 – 3451PhosphoserineBy similarity
    Modified residuei350 – 3501PhosphothreonineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiQ9Z2J4.
    PRIDEiQ9Z2J4.

    PTM databases

    PhosphoSiteiQ9Z2J4.

    Expressioni

    Tissue specificityi

    Expressed in brain, testis, spleen and fibroblasts (at protein level). Not detected in liver, kidney or epithelial cells (at protein level).1 Publication

    Gene expression databases

    GenevestigatoriQ9Z2J4.

    Interactioni

    Subunit structurei

    Interacts with F-actin.1 Publication

    Structurei

    3D structure databases

    ProteinModelPortaliQ9Z2J4.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini562 – 65089Ig-likeSequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi43 – 555513Glu-richSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Contains 1 Ig-like (immunoglobulin-like) domain.Sequence Analysis

    Keywords - Domaini

    Immunoglobulin domain

    Phylogenomic databases

    eggNOGiNOG147699.
    GeneTreeiENSGT00730000111176.
    HOGENOMiHOG000285935.
    HOVERGENiHBG066529.
    OMAiNFHEEED.
    OrthoDBiEOG7TBC1Z.
    PhylomeDBiQ9Z2J4.

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    InterProiIPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR013098. Ig_I-set.
    IPR003599. Ig_sub.
    IPR020675. Myosin_light_ch_kinase-rel.
    [Graphical view]
    PANTHERiPTHR22964. PTHR22964. 1 hit.
    PfamiPF07679. I-set. 1 hit.
    [Graphical view]
    SMARTiSM00409. IG. 1 hit.
    [Graphical view]
    PROSITEiPS50835. IG_LIKE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 11 Publication (identifier: Q9Z2J4-1) [UniParc]FASTAAdd to Basket

    Also known as: b-Nexilin1 Publication

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MNDVSQKAEI LLSSSKPVPK SYVPKLGKGD VKDKFEAMQR AREERNQRRS    50
    RDEKQRRKEQ YIREREWNRR KQEIKDMLAS DEEEEPSKVE KAYVPKLTGT 100
    VKGKFDEMEK HRQEEQRKRT EEERKRRIEQ DLLEKRKMQR ELAKRAEQEG 150
    DDSLLITVVP AKSYRAAANR KDPEDLDREH RNGRVSQEEE KTRHEEECRA 200
    LKEAKCLSLV MDDETEAKKE SRFPGKLKST FEELERQRQE NRKKQAEEEA 250
    RRRLEEERRA FEEARRNMVN EEDESQDTET VFKEYRPGKL RLSFEEIERQ 300
    RREEEKRKAE EEARRRMEEE KKAFAEARRS MVLDDDSPEI YKAVSQESLT 350
    PGKLEINFEQ LLRQKMEEER RRTEEERRQK LEMEKQEFEQ LRQEMGKEEE 400
    ENESFGLSRE YEELIKLKRS GSIQAKNLKS KFEKIGQLSE KEVQKKIEEE 450
    RAKRRAIDLE IKEREAENFH EDDDVDVKPA KKSESPFTHK VNMKARFEQM 500
    AKARQEEEQR RIEEQKLLRM QFEQKEIDAA LQKKREDDEE EEGSIVNGST 550
    TEDEEQTRSG APWFKKPLRN TSVVDSEPVR FTVKVTGEPK PEVTWWFEGE 600
    LLQDGEDYQY IERGETYCLY LPETFPEDGG EYMCKAVNSK GSAASTCILT 650
    IEMDDY 656
    Length:656
    Mass (Da):78,393
    Last modified:May 1, 1999 - v1
    Checksum:i98A4F2AF0396A7F2
    GO
    Isoform 21 Publication (identifier: Q9Z2J4-2) [UniParc]FASTAAdd to Basket

    Also known as: s-Nexilin1 Publication

    The sequence of this isoform differs from the canonical sequence as follows:
         11-74: Missing.
         148-148: Q → QIEDINNTGTESASE

    Show »
    Length:606
    Mass (Da):71,943
    Checksum:iD08CC05CE6902F3E
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei11 – 7464Missing in isoform 2. 1 PublicationVSP_052528Add
    BLAST
    Alternative sequencei148 – 1481Q → QIEDINNTGTESASE in isoform 2. 1 PublicationVSP_052529

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF056034 mRNA. Translation: AAC95128.1.
    AF056035 mRNA. Translation: AAC95129.1.
    RefSeqiNP_631976.1. NM_139230.1. [Q9Z2J4-1]
    NP_631977.1. NM_139231.1. [Q9Z2J4-2]
    XP_006233545.1. XM_006233483.1. [Q9Z2J4-1]
    XP_006233546.1. XM_006233484.1. [Q9Z2J4-2]
    UniGeneiRn.107975.

    Genome annotation databases

    EnsembliENSRNOT00000016967; ENSRNOP00000016968; ENSRNOG00000012512. [Q9Z2J4-1]
    ENSRNOT00000066780; ENSRNOP00000058811; ENSRNOG00000012512. [Q9Z2J4-2]
    GeneIDi246172.
    KEGGirno:246172.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF056034 mRNA. Translation: AAC95128.1 .
    AF056035 mRNA. Translation: AAC95129.1 .
    RefSeqi NP_631976.1. NM_139230.1. [Q9Z2J4-1 ]
    NP_631977.1. NM_139231.1. [Q9Z2J4-2 ]
    XP_006233545.1. XM_006233483.1. [Q9Z2J4-1 ]
    XP_006233546.1. XM_006233484.1. [Q9Z2J4-2 ]
    UniGenei Rn.107975.

    3D structure databases

    ProteinModelPortali Q9Z2J4.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q9Z2J4.

    Proteomic databases

    PaxDbi Q9Z2J4.
    PRIDEi Q9Z2J4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000016967 ; ENSRNOP00000016968 ; ENSRNOG00000012512 . [Q9Z2J4-1 ]
    ENSRNOT00000066780 ; ENSRNOP00000058811 ; ENSRNOG00000012512 . [Q9Z2J4-2 ]
    GeneIDi 246172.
    KEGGi rno:246172.

    Organism-specific databases

    CTDi 91624.
    RGDi 708354. Nexn.

    Phylogenomic databases

    eggNOGi NOG147699.
    GeneTreei ENSGT00730000111176.
    HOGENOMi HOG000285935.
    HOVERGENi HBG066529.
    OMAi NFHEEED.
    OrthoDBi EOG7TBC1Z.
    PhylomeDBi Q9Z2J4.

    Miscellaneous databases

    NextBioi 623456.
    PROi Q9Z2J4.

    Gene expression databases

    Genevestigatori Q9Z2J4.

    Family and domain databases

    Gene3Di 2.60.40.10. 1 hit.
    InterProi IPR007110. Ig-like_dom.
    IPR013783. Ig-like_fold.
    IPR013098. Ig_I-set.
    IPR003599. Ig_sub.
    IPR020675. Myosin_light_ch_kinase-rel.
    [Graphical view ]
    PANTHERi PTHR22964. PTHR22964. 1 hit.
    Pfami PF07679. I-set. 1 hit.
    [Graphical view ]
    SMARTi SM00409. IG. 1 hit.
    [Graphical view ]
    PROSITEi PS50835. IG_LIKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nexilin: a novel actin filament-binding protein localized at cell-matrix adherens junction."
      Ohtsuka T., Nakanishi H., Ikeda W., Satoh A., Momose Y., Nishioka H., Takai Y.
      J. Cell Biol. 143:1227-1238(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), INTERACTION WITH F-ACTIN, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    2. Cited for: SUBCELLULAR LOCATION.

    Entry informationi

    Entry nameiNEXN_RAT
    AccessioniPrimary (citable) accession number: Q9Z2J4
    Secondary accession number(s): Q9Z2J3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 11, 2007
    Last sequence update: May 1, 1999
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3