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Q9Z2J4

- NEXN_RAT

UniProt

Q9Z2J4 - NEXN_RAT

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Protein

Nexilin

Gene
Nexn
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in regulating cell migration through association with the actin cytoskeleton By similarity. Has an essential role in the maintenance of Z line and sarcomere integrity By similarity.By similarity

GO - Molecular functioni

  1. actin filament binding Source: RGD
  2. calmodulin-dependent protein kinase activity Source: RefGenome
  3. structural constituent of muscle Source: UniProtKB

GO - Biological processi

  1. cardiac muscle fiber development Source: RefGenome
  2. protein phosphorylation Source: GOC
  3. regulation of cell migration Source: UniProtKB
  4. regulation of cytoskeleton organization Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nexilin
Gene namesi
Name:Nexn
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 2

Organism-specific databases

RGDi708354. Nexn.

Subcellular locationi

Cytoplasmcytoskeleton. Cell junctionadherens junction. CytoplasmmyofibrilsarcomereZ line
Note: Localizes to the cell-matrix AJ. Not found at the cell-cell AJ.2 Publications

GO - Cellular componenti

  1. cell-substrate adherens junction Source: UniProtKB
  2. cytoskeleton Source: UniProtKB-SubCell
  3. Z disc Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 656656NexilinPRO_0000302087Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei80 – 801Phosphoserine By similarityBy similarity
Modified residuei221 – 2211Phosphoserine By similarity
Modified residuei337 – 3371Phosphoserine By similarity
Modified residuei345 – 3451Phosphoserine By similarityBy similarity
Modified residuei350 – 3501Phosphothreonine By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9Z2J4.
PRIDEiQ9Z2J4.

PTM databases

PhosphoSiteiQ9Z2J4.

Expressioni

Tissue specificityi

Expressed in brain, testis, spleen and fibroblasts (at protein level). Not detected in liver, kidney or epithelial cells (at protein level).1 Publication

Gene expression databases

GenevestigatoriQ9Z2J4.

Interactioni

Subunit structurei

Interacts with F-actin.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ9Z2J4.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini562 – 65089Ig-likeAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi43 – 555513Glu-richAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain

Phylogenomic databases

eggNOGiNOG147699.
GeneTreeiENSGT00730000111176.
HOGENOMiHOG000285935.
HOVERGENiHBG066529.
OMAiNFHEEED.
OrthoDBiEOG7TBC1Z.
PhylomeDBiQ9Z2J4.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR020675. Myosin_light_ch_kinase-rel.
[Graphical view]
PANTHERiPTHR22964. PTHR22964. 1 hit.
PfamiPF07679. I-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 11 Publication (identifier: Q9Z2J4-1) [UniParc]FASTAAdd to Basket

Also known as: b-Nexilin

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MNDVSQKAEI LLSSSKPVPK SYVPKLGKGD VKDKFEAMQR AREERNQRRS    50
RDEKQRRKEQ YIREREWNRR KQEIKDMLAS DEEEEPSKVE KAYVPKLTGT 100
VKGKFDEMEK HRQEEQRKRT EEERKRRIEQ DLLEKRKMQR ELAKRAEQEG 150
DDSLLITVVP AKSYRAAANR KDPEDLDREH RNGRVSQEEE KTRHEEECRA 200
LKEAKCLSLV MDDETEAKKE SRFPGKLKST FEELERQRQE NRKKQAEEEA 250
RRRLEEERRA FEEARRNMVN EEDESQDTET VFKEYRPGKL RLSFEEIERQ 300
RREEEKRKAE EEARRRMEEE KKAFAEARRS MVLDDDSPEI YKAVSQESLT 350
PGKLEINFEQ LLRQKMEEER RRTEEERRQK LEMEKQEFEQ LRQEMGKEEE 400
ENESFGLSRE YEELIKLKRS GSIQAKNLKS KFEKIGQLSE KEVQKKIEEE 450
RAKRRAIDLE IKEREAENFH EDDDVDVKPA KKSESPFTHK VNMKARFEQM 500
AKARQEEEQR RIEEQKLLRM QFEQKEIDAA LQKKREDDEE EEGSIVNGST 550
TEDEEQTRSG APWFKKPLRN TSVVDSEPVR FTVKVTGEPK PEVTWWFEGE 600
LLQDGEDYQY IERGETYCLY LPETFPEDGG EYMCKAVNSK GSAASTCILT 650
IEMDDY 656
Length:656
Mass (Da):78,393
Last modified:May 1, 1999 - v1
Checksum:i98A4F2AF0396A7F2
GO
Isoform 21 Publication (identifier: Q9Z2J4-2) [UniParc]FASTAAdd to Basket

Also known as: s-Nexilin

The sequence of this isoform differs from the canonical sequence as follows:
     11-74: Missing.
     148-148: Q → QIEDINNTGTESASE

Show »
Length:606
Mass (Da):71,943
Checksum:iD08CC05CE6902F3E
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei11 – 7464Missing in isoform 2. 1 PublicationVSP_052528Add
BLAST
Alternative sequencei148 – 1481Q → QIEDINNTGTESASE in isoform 2. 1 PublicationVSP_052529

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF056034 mRNA. Translation: AAC95128.1.
AF056035 mRNA. Translation: AAC95129.1.
RefSeqiNP_631976.1. NM_139230.1. [Q9Z2J4-1]
NP_631977.1. NM_139231.1. [Q9Z2J4-2]
XP_006233545.1. XM_006233483.1. [Q9Z2J4-1]
XP_006233546.1. XM_006233484.1. [Q9Z2J4-2]
UniGeneiRn.107975.

Genome annotation databases

EnsembliENSRNOT00000016967; ENSRNOP00000016968; ENSRNOG00000012512. [Q9Z2J4-1]
ENSRNOT00000066780; ENSRNOP00000058811; ENSRNOG00000012512. [Q9Z2J4-2]
GeneIDi246172.
KEGGirno:246172.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF056034 mRNA. Translation: AAC95128.1 .
AF056035 mRNA. Translation: AAC95129.1 .
RefSeqi NP_631976.1. NM_139230.1. [Q9Z2J4-1 ]
NP_631977.1. NM_139231.1. [Q9Z2J4-2 ]
XP_006233545.1. XM_006233483.1. [Q9Z2J4-1 ]
XP_006233546.1. XM_006233484.1. [Q9Z2J4-2 ]
UniGenei Rn.107975.

3D structure databases

ProteinModelPortali Q9Z2J4.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei Q9Z2J4.

Proteomic databases

PaxDbi Q9Z2J4.
PRIDEi Q9Z2J4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000016967 ; ENSRNOP00000016968 ; ENSRNOG00000012512 . [Q9Z2J4-1 ]
ENSRNOT00000066780 ; ENSRNOP00000058811 ; ENSRNOG00000012512 . [Q9Z2J4-2 ]
GeneIDi 246172.
KEGGi rno:246172.

Organism-specific databases

CTDi 91624.
RGDi 708354. Nexn.

Phylogenomic databases

eggNOGi NOG147699.
GeneTreei ENSGT00730000111176.
HOGENOMi HOG000285935.
HOVERGENi HBG066529.
OMAi NFHEEED.
OrthoDBi EOG7TBC1Z.
PhylomeDBi Q9Z2J4.

Miscellaneous databases

NextBioi 623456.
PROi Q9Z2J4.

Gene expression databases

Genevestigatori Q9Z2J4.

Family and domain databases

Gene3Di 2.60.40.10. 1 hit.
InterProi IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR020675. Myosin_light_ch_kinase-rel.
[Graphical view ]
PANTHERi PTHR22964. PTHR22964. 1 hit.
Pfami PF07679. I-set. 1 hit.
[Graphical view ]
SMARTi SM00409. IG. 1 hit.
[Graphical view ]
PROSITEi PS50835. IG_LIKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nexilin: a novel actin filament-binding protein localized at cell-matrix adherens junction."
    Ohtsuka T., Nakanishi H., Ikeda W., Satoh A., Momose Y., Nishioka H., Takai Y.
    J. Cell Biol. 143:1227-1238(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), INTERACTION WITH F-ACTIN, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  2. Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiNEXN_RAT
AccessioniPrimary (citable) accession number: Q9Z2J4
Secondary accession number(s): Q9Z2J3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: May 1, 1999
Last modified: June 11, 2014
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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