Skip Header

Contribute Send feedback
Read comments (?) or add your own

Q9Z2J4 (NEXN_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nexilin
Gene names
Name:Nexn
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length656 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in regulating cell migration through association with the actin cytoskeleton By similarity. Has an essential role in the maintenance of Z line and sarcomere integrity By similarity. UniProtKB Q0ZGT2

Subunit structure

Interacts with F-actin. Ref.1

Subcellular location

Cytoplasm › cytoskeleton. Cell junction › adherens junction. Cytoplasm › myofibril › sarcomere › Z line. Note: Localizes to the cell-matrix AJ. Not found at the cell-cell AJ. Ref.1 Ref.2

Tissue specificity

Expressed in brain, testis, spleen and fibroblasts (at protein level). Not detected in liver, kidney or epithelial cells (at protein level). Ref.1

Sequence similarities

Contains 1 Ig-like (immunoglobulin-like) domain.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.1 (identifier: Q9Z2J4-1)

Also known as: b-Nexilin;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 Ref.1 (identifier: Q9Z2J4-2)

Also known as: s-Nexilin;

The sequence of this isoform differs from the canonical sequence as follows:
     11-74: Missing.
     148-148: Q → QIEDINNTGTESASE

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 656656Nexilin
PRO_0000302087

Regions

Domain562 – 65089Ig-like
Compositional bias43 – 555513Glu-rich

Amino acid modifications

Modified residue801Phosphoserine By similarity UniProtKB Q0ZGT2
Modified residue2211Phosphoserine By similarity
Modified residue3371Phosphoserine By similarity
Modified residue3451Phosphoserine By similarity UniProtKB Q0ZGT2
Modified residue3501Phosphothreonine By similarity

Natural variations

Alternative sequence11 – 7464Missing in isoform 2. Ref.1
VSP_052528
Alternative sequence1481Q → QIEDINNTGTESASE in isoform 2. Ref.1
VSP_052529

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 (b-Nexilin) [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 98A4F2AF0396A7F2

FASTA65678,393
        10         20         30         40         50         60 
MNDVSQKAEI LLSSSKPVPK SYVPKLGKGD VKDKFEAMQR AREERNQRRS RDEKQRRKEQ 

        70         80         90        100        110        120 
YIREREWNRR KQEIKDMLAS DEEEEPSKVE KAYVPKLTGT VKGKFDEMEK HRQEEQRKRT 

       130        140        150        160        170        180 
EEERKRRIEQ DLLEKRKMQR ELAKRAEQEG DDSLLITVVP AKSYRAAANR KDPEDLDREH 

       190        200        210        220        230        240 
RNGRVSQEEE KTRHEEECRA LKEAKCLSLV MDDETEAKKE SRFPGKLKST FEELERQRQE 

       250        260        270        280        290        300 
NRKKQAEEEA RRRLEEERRA FEEARRNMVN EEDESQDTET VFKEYRPGKL RLSFEEIERQ 

       310        320        330        340        350        360 
RREEEKRKAE EEARRRMEEE KKAFAEARRS MVLDDDSPEI YKAVSQESLT PGKLEINFEQ 

       370        380        390        400        410        420 
LLRQKMEEER RRTEEERRQK LEMEKQEFEQ LRQEMGKEEE ENESFGLSRE YEELIKLKRS 

       430        440        450        460        470        480 
GSIQAKNLKS KFEKIGQLSE KEVQKKIEEE RAKRRAIDLE IKEREAENFH EDDDVDVKPA 

       490        500        510        520        530        540 
KKSESPFTHK VNMKARFEQM AKARQEEEQR RIEEQKLLRM QFEQKEIDAA LQKKREDDEE 

       550        560        570        580        590        600 
EEGSIVNGST TEDEEQTRSG APWFKKPLRN TSVVDSEPVR FTVKVTGEPK PEVTWWFEGE 

       610        620        630        640        650 
LLQDGEDYQY IERGETYCLY LPETFPEDGG EYMCKAVNSK GSAASTCILT IEMDDY

« Hide

Isoform 2 (s-Nexilin) [UniParc].

Checksum: D08CC05CE6902F3E
Show »

FASTA60671,943

References

[1]"Nexilin: a novel actin filament-binding protein localized at cell-matrix adherens junction."
Ohtsuka T., Nakanishi H., Ikeda W., Satoh A., Momose Y., Nishioka H., Takai Y.
J. Cell Biol. 143:1227-1238(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), INTERACTION WITH F-ACTIN, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
[2]"Nexilin mutations destabilize cardiac Z-disks and lead to dilated cardiomyopathy."
Hassel D., Dahme T., Erdmann J., Meder B., Huge A., Stoll M., Just S., Hess A., Ehlermann P., Weichenhan D., Grimmler M., Liptau H., Hetzer R., Regitz-Zagrosek V., Fischer C., Nurnberg P., Schunkert H., Katus H.A., Rottbauer W.
Nat. Med. 15:1281-1288(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF056034 mRNA. Translation: AAC95128.1.
AF056035 mRNA. Translation: AAC95129.1.
IPIIPI00209938.
IPI00396852.
RefSeqNP_631976.1. NM_139230.1.
NP_631977.1. NM_139231.1.
UniGeneRn.107975.

3D structure databases

HSSPHSSP built from PDB template 1FHG based on UniProtKB P56276.
ProteinModelPortalQ9Z2J4.
ModBaseSearch...

PTM databases

PhosphoSiteQ9Z2J4.

Proteomic databases

PaxDbQ9Z2J4.
PRIDEQ9Z2J4.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000016967; ENSRNOP00000016968; ENSRNOG00000012512.
ENSRNOT00000066780; ENSRNOP00000058811; ENSRNOG00000012512.
GeneID246172.
KEGGrno:246172.

Organism-specific databases

CTD91624.
RGD708354. Nexn.

Phylogenomic databases

eggNOGNOG147699.
GeneTreeENSGT00700000104480.
HOGENOMHOG000285935.
HOVERGENHBG066529.
OrthoDBEOG4GF3F5.

Gene expression databases

ArrayExpressQ9Z2J4.
GenevestigatorQ9Z2J4.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
InterProIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR020675. Myosin_light_ch_kinase-rel.
IPR020678. Nexilin.
[Graphical view]
PANTHERPTHR22964. PTHR22964. 1 hit.
PTHR22964:SF4. PTHR22964:SF4. 1 hit.
PfamPF07679. I-set. 1 hit.
[Graphical view]
SMARTSM00409. IG. 1 hit.
[Graphical view]
PROSITEPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio623456.

Entry information

Entry nameNEXN_RAT
AccessionPrimary (citable) accession number: Q9Z2J4
Secondary accession number(s): Q9Z2J3
Entry history
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: May 1, 1999
Last modified: April 3, 2013
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents