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Protein

Nexilin

Gene

Nexn

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in regulating cell migration through association with the actin cytoskeleton. Has an essential role in the maintenance of Z line and sarcomere integrity.By similarity

GO - Molecular functioni

  1. actin filament binding Source: RGD
  2. calmodulin-dependent protein kinase activity Source: GO_Central
  3. structural constituent of muscle Source: UniProtKB

GO - Biological processi

  1. cardiac muscle fiber development Source: GO_Central
  2. protein phosphorylation Source: GOC
  3. regulation of cell migration Source: UniProtKB
  4. regulation of cytoskeleton organization Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Actin-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nexilin
Gene namesi
Name:NexnImported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 2

Organism-specific databases

RGDi708354. Nexn.

Subcellular locationi

Cytoplasmcytoskeleton 1 Publication. Cell junctionadherens junction 1 Publication. CytoplasmmyofibrilsarcomereZ line
Note: Localizes to the cell-matrix AJ. Not found at the cell-cell AJ.1 Publication

GO - Cellular componenti

  1. cell-substrate adherens junction Source: UniProtKB
  2. cytoskeleton Source: UniProtKB-SubCell
  3. Z disc Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 656656NexilinPRO_0000302087Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei80 – 801PhosphoserineBy similarity
Modified residuei221 – 2211PhosphoserineBy similarity
Modified residuei337 – 3371PhosphoserineBy similarity
Modified residuei345 – 3451PhosphoserineBy similarity
Modified residuei350 – 3501PhosphothreonineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ9Z2J4.
PRIDEiQ9Z2J4.

PTM databases

PhosphoSiteiQ9Z2J4.

Expressioni

Tissue specificityi

Expressed in brain, testis, spleen and fibroblasts (at protein level). Not detected in liver, kidney or epithelial cells (at protein level).1 Publication

Gene expression databases

GenevestigatoriQ9Z2J4.

Interactioni

Subunit structurei

Interacts with F-actin.1 Publication

Structurei

3D structure databases

ProteinModelPortaliQ9Z2J4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini562 – 65089Ig-likeSequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi43 – 555513Glu-richSequence AnalysisAdd
BLAST

Sequence similaritiesi

Contains 1 Ig-like (immunoglobulin-like) domain.Sequence Analysis

Keywords - Domaini

Immunoglobulin domain

Phylogenomic databases

eggNOGiNOG147699.
GeneTreeiENSGT00730000111176.
HOGENOMiHOG000285935.
HOVERGENiHBG066529.
InParanoidiQ9Z2J4.
OMAiNFHEEED.
OrthoDBiEOG7TBC1Z.
PhylomeDBiQ9Z2J4.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR020675. Myosin_light_ch_kinase-rel.
[Graphical view]
PANTHERiPTHR22964. PTHR22964. 1 hit.
PfamiPF07679. I-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 11 Publication (identifier: Q9Z2J4-1) [UniParc]FASTAAdd to Basket

Also known as: b-Nexilin1 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNDVSQKAEI LLSSSKPVPK SYVPKLGKGD VKDKFEAMQR AREERNQRRS
60 70 80 90 100
RDEKQRRKEQ YIREREWNRR KQEIKDMLAS DEEEEPSKVE KAYVPKLTGT
110 120 130 140 150
VKGKFDEMEK HRQEEQRKRT EEERKRRIEQ DLLEKRKMQR ELAKRAEQEG
160 170 180 190 200
DDSLLITVVP AKSYRAAANR KDPEDLDREH RNGRVSQEEE KTRHEEECRA
210 220 230 240 250
LKEAKCLSLV MDDETEAKKE SRFPGKLKST FEELERQRQE NRKKQAEEEA
260 270 280 290 300
RRRLEEERRA FEEARRNMVN EEDESQDTET VFKEYRPGKL RLSFEEIERQ
310 320 330 340 350
RREEEKRKAE EEARRRMEEE KKAFAEARRS MVLDDDSPEI YKAVSQESLT
360 370 380 390 400
PGKLEINFEQ LLRQKMEEER RRTEEERRQK LEMEKQEFEQ LRQEMGKEEE
410 420 430 440 450
ENESFGLSRE YEELIKLKRS GSIQAKNLKS KFEKIGQLSE KEVQKKIEEE
460 470 480 490 500
RAKRRAIDLE IKEREAENFH EDDDVDVKPA KKSESPFTHK VNMKARFEQM
510 520 530 540 550
AKARQEEEQR RIEEQKLLRM QFEQKEIDAA LQKKREDDEE EEGSIVNGST
560 570 580 590 600
TEDEEQTRSG APWFKKPLRN TSVVDSEPVR FTVKVTGEPK PEVTWWFEGE
610 620 630 640 650
LLQDGEDYQY IERGETYCLY LPETFPEDGG EYMCKAVNSK GSAASTCILT

IEMDDY
Length:656
Mass (Da):78,393
Last modified:May 1, 1999 - v1
Checksum:i98A4F2AF0396A7F2
GO
Isoform 21 Publication (identifier: Q9Z2J4-2) [UniParc]FASTAAdd to Basket

Also known as: s-Nexilin1 Publication

The sequence of this isoform differs from the canonical sequence as follows:
     11-74: Missing.
     148-148: Q → QIEDINNTGTESASE

Show »
Length:606
Mass (Da):71,943
Checksum:iD08CC05CE6902F3E
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei11 – 7464Missing in isoform 2. 1 PublicationVSP_052528Add
BLAST
Alternative sequencei148 – 1481Q → QIEDINNTGTESASE in isoform 2. 1 PublicationVSP_052529

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF056034 mRNA. Translation: AAC95128.1.
AF056035 mRNA. Translation: AAC95129.1.
RefSeqiNP_631976.1. NM_139230.1. [Q9Z2J4-1]
NP_631977.1. NM_139231.1. [Q9Z2J4-2]
XP_006233545.1. XM_006233483.2. [Q9Z2J4-1]
XP_006233546.1. XM_006233484.2. [Q9Z2J4-2]
UniGeneiRn.107975.

Genome annotation databases

EnsembliENSRNOT00000016967; ENSRNOP00000016968; ENSRNOG00000012512. [Q9Z2J4-1]
ENSRNOT00000066780; ENSRNOP00000058811; ENSRNOG00000012512. [Q9Z2J4-2]
GeneIDi246172.
KEGGirno:246172.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF056034 mRNA. Translation: AAC95128.1.
AF056035 mRNA. Translation: AAC95129.1.
RefSeqiNP_631976.1. NM_139230.1. [Q9Z2J4-1]
NP_631977.1. NM_139231.1. [Q9Z2J4-2]
XP_006233545.1. XM_006233483.2. [Q9Z2J4-1]
XP_006233546.1. XM_006233484.2. [Q9Z2J4-2]
UniGeneiRn.107975.

3D structure databases

ProteinModelPortaliQ9Z2J4.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ9Z2J4.

Proteomic databases

PaxDbiQ9Z2J4.
PRIDEiQ9Z2J4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000016967; ENSRNOP00000016968; ENSRNOG00000012512. [Q9Z2J4-1]
ENSRNOT00000066780; ENSRNOP00000058811; ENSRNOG00000012512. [Q9Z2J4-2]
GeneIDi246172.
KEGGirno:246172.

Organism-specific databases

CTDi91624.
RGDi708354. Nexn.

Phylogenomic databases

eggNOGiNOG147699.
GeneTreeiENSGT00730000111176.
HOGENOMiHOG000285935.
HOVERGENiHBG066529.
InParanoidiQ9Z2J4.
OMAiNFHEEED.
OrthoDBiEOG7TBC1Z.
PhylomeDBiQ9Z2J4.

Miscellaneous databases

NextBioi623456.
PROiQ9Z2J4.

Gene expression databases

GenevestigatoriQ9Z2J4.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013098. Ig_I-set.
IPR003599. Ig_sub.
IPR020675. Myosin_light_ch_kinase-rel.
[Graphical view]
PANTHERiPTHR22964. PTHR22964. 1 hit.
PfamiPF07679. I-set. 1 hit.
[Graphical view]
SMARTiSM00409. IG. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nexilin: a novel actin filament-binding protein localized at cell-matrix adherens junction."
    Ohtsuka T., Nakanishi H., Ikeda W., Satoh A., Momose Y., Nishioka H., Takai Y.
    J. Cell Biol. 143:1227-1238(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), INTERACTION WITH F-ACTIN, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
  2. Cited for: SUBCELLULAR LOCATION.

Entry informationi

Entry nameiNEXN_RAT
AccessioniPrimary (citable) accession number: Q9Z2J4
Secondary accession number(s): Q9Z2J3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: May 1, 1999
Last modified: February 4, 2015
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.