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Protein

Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial

Gene

Sucla2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

ATP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of ATP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.UniRule annotation

Catalytic activityi

ATP + succinate + CoA = ADP + phosphate + succinyl-CoA.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes succinate from succinyl-CoA (ligase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial (Suclg2), Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (Sucla2), Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial (Suclg1), Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial (Suclg2)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes succinate from succinyl-CoA (ligase route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei94Important for substrate specificityUniRule annotation1
Binding sitei98ATPUniRule annotation1
Sitei162Important for substrate specificityUniRule annotation1
Metal bindingi258MagnesiumUniRule annotation1
Metal bindingi272MagnesiumUniRule annotation1
Binding sitei323Substrate; shared with subunit alphaUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi105 – 107ATPUniRule annotation3

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • succinate-CoA ligase (ADP-forming) activity Source: MGI
  • succinate-CoA ligase activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processTricarboxylic acid cycle
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-71403 Citric acid cycle (TCA cycle)
UniPathwayiUPA00223; UER00999

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrialUniRule annotation (EC:6.2.1.5UniRule annotation)
Alternative name(s):
ATP-specific succinyl-CoA synthetase subunit betaUniRule annotation
Short name:
A-SCSUniRule annotation
Succinyl-CoA synthetase beta-A chainUniRule annotation
Short name:
SCS-betaAUniRule annotation
Gene namesi
Name:Sucla2UniRule annotation
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1306775 Sucla2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 53MitochondrionUniRule annotationAdd BLAST53
ChainiPRO_000003335354 – 463Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrialUniRule annotationAdd BLAST410

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei78N6-acetyllysineCombined sources1
Modified residuei84PhosphotyrosineBy similarity1
Modified residuei88N6-acetyllysine; alternateCombined sources1
Modified residuei88N6-succinyllysine; alternateCombined sources1
Modified residuei129N6-acetyllysineCombined sources1
Modified residuei139N6-acetyllysineCombined sources1
Modified residuei143N6-acetyllysineBy similarity1
Modified residuei216N6-acetyllysineCombined sources1
Modified residuei279PhosphoserineCombined sources1
Modified residuei341PhosphothreonineCombined sources1
Modified residuei368N6-acetyllysineCombined sources1
Modified residuei438N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9Z2I9
MaxQBiQ9Z2I9
PaxDbiQ9Z2I9
PeptideAtlasiQ9Z2I9
PRIDEiQ9Z2I9

2D gel databases

REPRODUCTION-2DPAGEiIPI00261627
Q9Z2I9
UCD-2DPAGEiQ9Z2I9

PTM databases

iPTMnetiQ9Z2I9
PhosphoSitePlusiQ9Z2I9
SwissPalmiQ9Z2I9

Expressioni

Gene expression databases

BgeeiENSMUSG00000022110
GenevisibleiQ9Z2I9 MM

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. The beta subunit determines specificity for ATP.UniRule annotation

Protein-protein interaction databases

BioGridi203570, 2 interactors
CORUMiQ9Z2I9
IntActiQ9Z2I9, 11 interactors
MINTiQ9Z2I9
STRINGi10090.ENSMUSP00000022706

Structurei

3D structure databases

ProteinModelPortaliQ9Z2I9
SMRiQ9Z2I9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini61 – 288ATP-graspUniRule annotationAdd BLAST228

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni380 – 382Substrate binding; shared with subunit alphaUniRule annotation3

Sequence similaritiesi

Belongs to the succinate/malate CoA ligase beta subunit family. ATP-specific subunit beta subfamily.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2799 Eukaryota
COG0045 LUCA
GeneTreeiENSGT00390000010170
HOVERGENiHBG055555
InParanoidiQ9Z2I9
KOiK01900
OMAiLCMDAKF
OrthoDBiEOG091G07T9
PhylomeDBiQ9Z2I9
TreeFamiTF300624

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
3.40.50.261, 1 hit
HAMAPiMF_00558 Succ_CoA_beta, 1 hit
MF_03220 Succ_CoA_betaA_euk, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013650 ATP-grasp_succ-CoA_synth-type
IPR013815 ATP_grasp_subdomain_1
IPR005811 CoA_ligase
IPR017866 Succ-CoA_synthase_bsu_CS
IPR034723 Succ_CoA_betaA_euk
IPR005809 Succ_CoA_synthase_bsu
IPR016102 Succinyl-CoA_synth-like
PANTHERiPTHR11815 PTHR11815, 1 hit
PfamiView protein in Pfam
PF08442 ATP-grasp_2, 1 hit
PF00549 Ligase_CoA, 1 hit
PIRSFiPIRSF001554 SucCS_beta, 1 hit
SUPFAMiSSF52210 SSF52210, 1 hit
TIGRFAMsiTIGR01016 sucCoAbeta, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS01217 SUCCINYL_COA_LIG_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z2I9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAASMFYGRQ LAAAALRSHR PQTTLRAAAQ VLGNSGLFNK HGLQVQQQQQ
60 70 80 90 100
RTLSLHEYLS MELLQEAGVS VPKGFVAKSS DEAYAIAKKL GSKDVVIKAQ
110 120 130 140 150
VLAGGRGKGT FTSGLKGGVK IVFSPEEAKA VSSQMIGQKL ITKQTGEKGR
160 170 180 190 200
ICNQVLVCER KYPRREYYFA ITMERSFQGP VLIGSAQGGV NIEDVAAENP
210 220 230 240 250
EAIVKEPIDI VEGIKKEQAV TLAQKMGFPS NIVDSAAENM IKLYNLFLKY
260 270 280 290 300
DATMVEINPM VEDSDGKVLC MDAKINFDSN SAYRQKKIFD LQDWSQEDER
310 320 330 340 350
DKEAANADIN YIGLDGSIGC LVNGAGLAMA TMDIIKLHGG TPANFLDVGG
360 370 380 390 400
GATVQQVTEA FKLITSDKKV QAILVNIFGG IMRCDVIAQG IVMAVKDLEI
410 420 430 440 450
RIPVVVRLQG TRVDDAKALI ADSGLKILAC DDLDEAAKMV VKLSEIVTLA
460
KEAHVDVKFQ LPI
Length:463
Mass (Da):50,114
Last modified:September 27, 2004 - v2
Checksum:i74CE8E962BC0BB27
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK081965 mRNA Translation: BAC38380.1
AK088801 mRNA Translation: BAC40580.1
AK132762 mRNA Translation: BAE21343.1
AK160130 mRNA Translation: BAE35647.1
AK160652 mRNA Translation: BAE35942.1
BC056353 mRNA Translation: AAH56353.1
BC057605 mRNA Translation: AAH57605.1
AF058955 mRNA Translation: AAC64398.1
CCDSiCCDS27271.1
RefSeqiNP_035636.1, NM_011506.3
UniGeneiMm.38951

Genome annotation databases

EnsembliENSMUST00000022706; ENSMUSP00000022706; ENSMUSG00000022110
ENSMUST00000160507; ENSMUSP00000123765; ENSMUSG00000022110
GeneIDi20916
KEGGimmu:20916
UCSCiuc007upx.1 mouse

Similar proteinsi

Entry informationi

Entry nameiSUCB1_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2I9
Secondary accession number(s): Q3TVH1, Q8BGS6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: September 27, 2004
Last modified: June 20, 2018
This is version 162 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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