Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial

Gene

Suclg2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

GTP-specific succinyl-CoA synthetase functions in the citric acid cycle (TCA), coupling the hydrolysis of succinyl-CoA to the synthesis of GTP and thus represents the only step of substrate-level phosphorylation in the TCA. The beta subunit provides nucleotide specificity of the enzyme and binds the substrate succinate, while the binding sites for coenzyme A and phosphate are found in the alpha subunit.UniRule annotation

Catalytic activityi

GTP + succinate + CoA = GDP + phosphate + succinyl-CoA.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 1 of the subpathway that synthesizes succinate from succinyl-CoA (ligase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Succinate--CoA ligase [ADP-forming] subunit beta, mitochondrial (Sucla2), Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial (Suclg2), Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial (Suclg1)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes succinate from succinyl-CoA (ligase route), the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei58GTPUniRule annotation1
Sitei80Important for substrate specificityUniRule annotation1
Binding sitei147GTP; via amide nitrogen and carbonyl oxygenUniRule annotation1
Sitei148Important for substrate specificityUniRule annotation1
Metal bindingi244MagnesiumUniRule annotation1
Metal bindingi258MagnesiumUniRule annotation1
Binding sitei309Substrate; shared with subunit alphaUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi91 – 93GTPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-71403. Citric acid cycle (TCA cycle).
UniPathwayiUPA00223; UER00999.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrialUniRule annotation (EC:6.2.1.4UniRule annotation)
Alternative name(s):
GTP-specific succinyl-CoA synthetase subunit betaUniRule annotation
Short name:
G-SCSUniRule annotation
Short name:
GTPSCSUniRule annotation
Succinyl-CoA synthetase beta-G chainUniRule annotation
Short name:
SCS-betaGUniRule annotation
Gene namesi
Name:Suclg2UniRule annotation
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1306824. Suclg2.

Subcellular locationi

  • Mitochondrion UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 38MitochondrionBy similarityAdd BLAST38
ChainiPRO_000003335739 – 433Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrialAdd BLAST395

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei67N6-acetyllysine; alternateCombined sources1
Modified residuei67N6-succinyllysine; alternateCombined sources1
Modified residuei74N6-acetyllysineCombined sources1
Modified residuei79N6-succinyllysineCombined sources1
Modified residuei112N6-acetyllysineCombined sources1
Modified residuei133N6-acetyllysineCombined sources1
Modified residuei140N6-acetyllysineCombined sources1
Modified residuei162PhosphoserineCombined sources1
Modified residuei201N6-acetyllysineCombined sources1
Modified residuei217PhosphoserineCombined sources1
Modified residuei219N6-acetyllysineCombined sources1
Modified residuei228N6-acetyllysineCombined sources1
Modified residuei272N6-acetyllysineCombined sources1
Modified residuei339N6-succinyllysineCombined sources1
Modified residuei348N6-acetyllysineCombined sources1
Modified residuei387N6-acetyllysineCombined sources1
Modified residuei407N6-acetyllysineCombined sources1
Modified residuei424N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9Z2I8.
PeptideAtlasiQ9Z2I8.
PRIDEiQ9Z2I8.

2D gel databases

REPRODUCTION-2DPAGEQ9Z2I8.

PTM databases

iPTMnetiQ9Z2I8.
PhosphoSitePlusiQ9Z2I8.
SwissPalmiQ9Z2I8.

Expressioni

Gene expression databases

BgeeiENSMUSG00000061838.
ExpressionAtlasiQ9Z2I8. baseline and differential.
GenevisibleiQ9Z2I8. MM.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. The beta subunit determines specificity for GTP.UniRule annotation

Protein-protein interaction databases

BioGridi203571. 1 interactor.
IntActiQ9Z2I8. 3 interactors.
MINTiMINT-1841325.
STRINGi10090.ENSMUSP00000078774.

Structurei

3D structure databases

ProteinModelPortaliQ9Z2I8.
SMRiQ9Z2I8.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini47 – 275ATP-graspUniRule annotationAdd BLAST229

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni366 – 368Substrate binding; shared with subunit alphaUniRule annotation3

Sequence similaritiesi

Belongs to the succinate/malate CoA ligase beta subunit family. GTP-specific subunit beta subfamily.UniRule annotation
Contains 1 ATP-grasp domain.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2799. Eukaryota.
COG0045. LUCA.
GeneTreeiENSGT00390000010170.
HOVERGENiHBG055555.
InParanoidiQ9Z2I8.
KOiK01900.
OMAiDREHNGP.
OrthoDBiEOG091G07T9.
PhylomeDBiQ9Z2I8.
TreeFamiTF300624.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.261. 1 hit.
HAMAPiMF_00558. Succ_CoA_beta. 1 hit.
MF_03221. Succ_CoA_betaG_euk. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013650. ATP-grasp_succ-CoA_synth-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR005811. CoA_ligase.
IPR017866. Succ-CoA_synthase_bsu_CS.
IPR005809. Succ_CoA_synthase_bsu.
IPR016102. Succinyl-CoA_synth-like.
[Graphical view]
PANTHERiPTHR11815. PTHR11815. 1 hit.
PfamiPF08442. ATP-grasp_2. 1 hit.
PF00549. Ligase_CoA. 1 hit.
[Graphical view]
PIRSFiPIRSF001554. SucCS_beta. 1 hit.
SUPFAMiSSF52210. SSF52210. 1 hit.
TIGRFAMsiTIGR01016. sucCoAbeta. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS01217. SUCCINYL_COA_LIG_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Z2I8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASPVAIAAQ AGKLLRERAL RPLLAVRSQA GHLTPRRWLN LQEYQSKKLM
60 70 80 90 100
SEHGVRVQRF FVANTAKEAL EAAKRLNAKE IVLKAQILAG GRGKGVFNSG
110 120 130 140 150
LKGGVHLTKD PKVVGELAQQ MIGYNLATKQ TPKEGVKVNK VMVAEALDIS
160 170 180 190 200
RETYLAILMD RSHNGPVIVG SPQGGVDIEE VAASSPELIF KEQIDIFEGI
210 220 230 240 250
KDSQAQRMAE NLGFLGSLKN QAADQITKLY HLFLKIDATQ VEVNPFGETP
260 270 280 290 300
EGQVVCFDAK INFDDNAEFR QKDIFAMDDK SENEPIENEA ARYDLKYIGL
310 320 330 340 350
DGNIACFVNG AGLAMATCDI IFLNGGKPAN FLDLGGGVKE AQVYEAFKLL
360 370 380 390 400
TSDPKVEAIL VNIFGGIVNC AIIANGITKA CRELELKVPL VVRLEGTNVQ
410 420 430
EAQNILKSSG LPITSAVDLE DAAKKAVASV AKK
Length:433
Mass (Da):46,840
Last modified:January 10, 2006 - v3
Checksum:i8CA9C7998D2F2957
GO
Isoform 2 (identifier: Q9Z2I8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-49: Missing.

Note: No experimental confirmation available.
Show »
Length:384
Mass (Da):41,349
Checksum:i4EB7A1CB5F5A5E82
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti66A → T in BAE43106 (PubMed:16141072).Curated1
Sequence conflicti66A → T in BAE43068 (PubMed:16141072).Curated1
Sequence conflicti168I → L in AAC64399 (PubMed:9765291).Curated1
Sequence conflicti175 – 176GV → RS in AAC64399 (PubMed:9765291).Curated2
Sequence conflicti212L → P in AAH80781 (PubMed:15489334).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0169051 – 49Missing in isoform 2. 1 PublicationAdd BLAST49

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK167136 mRNA. Translation: BAE39282.1.
AK167339 mRNA. Translation: BAE39440.1.
AK172561 mRNA. Translation: BAE43068.1.
AK172633 mRNA. Translation: BAE43106.1.
BC030947 mRNA. Translation: AAH30947.1.
BC043312 mRNA. Translation: AAH43312.1.
BC054425 mRNA. Translation: AAH54425.1.
BC080781 mRNA. Translation: AAH80781.1.
AF058956 mRNA. Translation: AAC64399.1.
CCDSiCCDS20381.1. [Q9Z2I8-1]
RefSeqiNP_001313487.1. NM_001326558.1. [Q9Z2I8-2]
NP_035637.2. NM_011507.3. [Q9Z2I8-1]
UniGeneiMm.371585.
Mm.426727.

Genome annotation databases

EnsembliENSMUST00000079847; ENSMUSP00000078774; ENSMUSG00000061838. [Q9Z2I8-2]
ENSMUST00000204224; ENSMUSP00000144827; ENSMUSG00000061838. [Q9Z2I8-1]
GeneIDi20917.
KEGGimmu:20917.
UCSCiuc009daa.3. mouse. [Q9Z2I8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK167136 mRNA. Translation: BAE39282.1.
AK167339 mRNA. Translation: BAE39440.1.
AK172561 mRNA. Translation: BAE43068.1.
AK172633 mRNA. Translation: BAE43106.1.
BC030947 mRNA. Translation: AAH30947.1.
BC043312 mRNA. Translation: AAH43312.1.
BC054425 mRNA. Translation: AAH54425.1.
BC080781 mRNA. Translation: AAH80781.1.
AF058956 mRNA. Translation: AAC64399.1.
CCDSiCCDS20381.1. [Q9Z2I8-1]
RefSeqiNP_001313487.1. NM_001326558.1. [Q9Z2I8-2]
NP_035637.2. NM_011507.3. [Q9Z2I8-1]
UniGeneiMm.371585.
Mm.426727.

3D structure databases

ProteinModelPortaliQ9Z2I8.
SMRiQ9Z2I8.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203571. 1 interactor.
IntActiQ9Z2I8. 3 interactors.
MINTiMINT-1841325.
STRINGi10090.ENSMUSP00000078774.

PTM databases

iPTMnetiQ9Z2I8.
PhosphoSitePlusiQ9Z2I8.
SwissPalmiQ9Z2I8.

2D gel databases

REPRODUCTION-2DPAGEQ9Z2I8.

Proteomic databases

PaxDbiQ9Z2I8.
PeptideAtlasiQ9Z2I8.
PRIDEiQ9Z2I8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000079847; ENSMUSP00000078774; ENSMUSG00000061838. [Q9Z2I8-2]
ENSMUST00000204224; ENSMUSP00000144827; ENSMUSG00000061838. [Q9Z2I8-1]
GeneIDi20917.
KEGGimmu:20917.
UCSCiuc009daa.3. mouse. [Q9Z2I8-1]

Organism-specific databases

CTDi8801.
MGIiMGI:1306824. Suclg2.

Phylogenomic databases

eggNOGiKOG2799. Eukaryota.
COG0045. LUCA.
GeneTreeiENSGT00390000010170.
HOVERGENiHBG055555.
InParanoidiQ9Z2I8.
KOiK01900.
OMAiDREHNGP.
OrthoDBiEOG091G07T9.
PhylomeDBiQ9Z2I8.
TreeFamiTF300624.

Enzyme and pathway databases

UniPathwayiUPA00223; UER00999.
ReactomeiR-MMU-71403. Citric acid cycle (TCA cycle).

Miscellaneous databases

PROiQ9Z2I8.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000061838.
ExpressionAtlasiQ9Z2I8. baseline and differential.
GenevisibleiQ9Z2I8. MM.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.261. 1 hit.
HAMAPiMF_00558. Succ_CoA_beta. 1 hit.
MF_03221. Succ_CoA_betaG_euk. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013650. ATP-grasp_succ-CoA_synth-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR005811. CoA_ligase.
IPR017866. Succ-CoA_synthase_bsu_CS.
IPR005809. Succ_CoA_synthase_bsu.
IPR016102. Succinyl-CoA_synth-like.
[Graphical view]
PANTHERiPTHR11815. PTHR11815. 1 hit.
PfamiPF08442. ATP-grasp_2. 1 hit.
PF00549. Ligase_CoA. 1 hit.
[Graphical view]
PIRSFiPIRSF001554. SucCS_beta. 1 hit.
SUPFAMiSSF52210. SSF52210. 1 hit.
TIGRFAMsiTIGR01016. sucCoAbeta. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS01217. SUCCINYL_COA_LIG_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSUCB2_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2I8
Secondary accession number(s): Q3T9B8
, Q3TJQ5, Q3TK63, Q66JT3, Q7TMY3, Q80VV1, Q8K2K9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: January 10, 2006
Last modified: November 30, 2016
This is version 149 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.