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Protein

Succinyl-CoA ligase [GDP-forming] subunit beta, mitochondrial

Gene

Suclg2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the GTP-dependent ligation of succinate and CoA to form succinyl-CoA.By similarity

Catalytic activityi

GTP + succinate + CoA = GDP + phosphate + succinyl-CoA.

Pathwayi: tricarboxylic acid cycle

This protein is involved in the pathway tricarboxylic acid cycle, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Tricarboxylic acid cycle

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-71403. Citric acid cycle (TCA cycle).
UniPathwayiUPA00223.

Names & Taxonomyi

Protein namesi
Recommended name:
Succinyl-CoA ligase [GDP-forming] subunit beta, mitochondrial (EC:6.2.1.4)
Alternative name(s):
GTP-specific succinyl-CoA synthetase subunit beta
Succinyl-CoA synthetase beta-G chain
Short name:
SCS-betaG
Gene namesi
Name:Suclg2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:1306824. Suclg2.

Subcellular locationi

GO - Cellular componenti

  • microtubule organizing center Source: MGI
  • mitochondrion Source: MGI
  • nucleus Source: MGI
  • plasma membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3838MitochondrionBy similarityAdd
BLAST
Chaini39 – 433395Succinyl-CoA ligase [GDP-forming] subunit beta, mitochondrialPRO_0000033357Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei67 – 671N6-acetyllysine; alternateCombined sources
Modified residuei67 – 671N6-succinyllysine; alternateCombined sources
Modified residuei74 – 741N6-acetyllysineCombined sources
Modified residuei79 – 791N6-succinyllysineCombined sources
Modified residuei112 – 1121N6-acetyllysineCombined sources
Modified residuei133 – 1331N6-acetyllysineCombined sources
Modified residuei140 – 1401N6-acetyllysineCombined sources
Modified residuei162 – 1621PhosphoserineCombined sources
Modified residuei201 – 2011N6-acetyllysineCombined sources
Modified residuei217 – 2171PhosphoserineCombined sources
Modified residuei219 – 2191N6-acetyllysineCombined sources
Modified residuei228 – 2281N6-acetyllysineCombined sources
Modified residuei272 – 2721N6-acetyllysineCombined sources
Modified residuei339 – 3391N6-succinyllysineCombined sources
Modified residuei348 – 3481N6-acetyllysineCombined sources
Modified residuei387 – 3871N6-acetyllysineCombined sources
Modified residuei407 – 4071N6-acetyllysineCombined sources
Modified residuei424 – 4241N6-acetyllysineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9Z2I8.
MaxQBiQ9Z2I8.
PaxDbiQ9Z2I8.
PeptideAtlasiQ9Z2I8.
PRIDEiQ9Z2I8.

2D gel databases

REPRODUCTION-2DPAGEQ9Z2I8.

PTM databases

iPTMnetiQ9Z2I8.
PhosphoSiteiQ9Z2I8.
SwissPalmiQ9Z2I8.

Expressioni

Gene expression databases

BgeeiQ9Z2I8.
ExpressionAtlasiQ9Z2I8. baseline.
GenevisibleiQ9Z2I8. MM.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit.By similarity

Protein-protein interaction databases

IntActiQ9Z2I8. 3 interactions.
MINTiMINT-1841325.
STRINGi10090.ENSMUSP00000078774.

Structurei

3D structure databases

ProteinModelPortaliQ9Z2I8.
SMRiQ9Z2I8. Positions 39-430.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini47 – 275229ATP-graspPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ATP-grasp domain.PROSITE-ProRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG2799. Eukaryota.
COG0045. LUCA.
GeneTreeiENSGT00390000010170.
HOVERGENiHBG055555.
InParanoidiQ9Z2I8.
KOiK01900.
OMAiDREHNGP.
OrthoDBiEOG7CZK66.
PhylomeDBiQ9Z2I8.
TreeFamiTF300624.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.261. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013650. ATP-grasp_succ-CoA_synth-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR005811. CoA_ligase.
IPR017866. Succ-CoA_synthase_bsu_CS.
IPR005809. Succ_CoA_synthase_bsu.
IPR016102. Succinyl-CoA_synth-like.
[Graphical view]
PANTHERiPTHR11815. PTHR11815. 1 hit.
PfamiPF08442. ATP-grasp_2. 1 hit.
PF00549. Ligase_CoA. 1 hit.
[Graphical view]
PIRSFiPIRSF001554. SucCS_beta. 1 hit.
SUPFAMiSSF52210. SSF52210. 1 hit.
TIGRFAMsiTIGR01016. sucCoAbeta. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS01217. SUCCINYL_COA_LIG_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Z2I8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MASPVAIAAQ AGKLLRERAL RPLLAVRSQA GHLTPRRWLN LQEYQSKKLM
60 70 80 90 100
SEHGVRVQRF FVANTAKEAL EAAKRLNAKE IVLKAQILAG GRGKGVFNSG
110 120 130 140 150
LKGGVHLTKD PKVVGELAQQ MIGYNLATKQ TPKEGVKVNK VMVAEALDIS
160 170 180 190 200
RETYLAILMD RSHNGPVIVG SPQGGVDIEE VAASSPELIF KEQIDIFEGI
210 220 230 240 250
KDSQAQRMAE NLGFLGSLKN QAADQITKLY HLFLKIDATQ VEVNPFGETP
260 270 280 290 300
EGQVVCFDAK INFDDNAEFR QKDIFAMDDK SENEPIENEA ARYDLKYIGL
310 320 330 340 350
DGNIACFVNG AGLAMATCDI IFLNGGKPAN FLDLGGGVKE AQVYEAFKLL
360 370 380 390 400
TSDPKVEAIL VNIFGGIVNC AIIANGITKA CRELELKVPL VVRLEGTNVQ
410 420 430
EAQNILKSSG LPITSAVDLE DAAKKAVASV AKK
Length:433
Mass (Da):46,840
Last modified:January 10, 2006 - v3
Checksum:i8CA9C7998D2F2957
GO
Isoform 2 (identifier: Q9Z2I8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-49: Missing.

Note: No experimental confirmation available.
Show »
Length:384
Mass (Da):41,349
Checksum:i4EB7A1CB5F5A5E82
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti66 – 661A → T in BAE43106 (PubMed:16141072).Curated
Sequence conflicti66 – 661A → T in BAE43068 (PubMed:16141072).Curated
Sequence conflicti168 – 1681I → L in AAC64399 (PubMed:9765291).Curated
Sequence conflicti175 – 1762GV → RS in AAC64399 (PubMed:9765291).Curated
Sequence conflicti212 – 2121L → P in AAH80781 (PubMed:15489334).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 4949Missing in isoform 2. 1 PublicationVSP_016905Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK167136 mRNA. Translation: BAE39282.1.
AK167339 mRNA. Translation: BAE39440.1.
AK172561 mRNA. Translation: BAE43068.1.
AK172633 mRNA. Translation: BAE43106.1.
BC030947 mRNA. Translation: AAH30947.1.
BC043312 mRNA. Translation: AAH43312.1.
BC054425 mRNA. Translation: AAH54425.1.
BC080781 mRNA. Translation: AAH80781.1.
AF058956 mRNA. Translation: AAC64399.1.
CCDSiCCDS20381.1. [Q9Z2I8-1]
RefSeqiNP_035637.2. NM_011507.3. [Q9Z2I8-1]
UniGeneiMm.371585.
Mm.426727.

Genome annotation databases

EnsembliENSMUST00000079847; ENSMUSP00000078774; ENSMUSG00000061838. [Q9Z2I8-2]
ENSMUST00000204224; ENSMUSP00000144827; ENSMUSG00000061838. [Q9Z2I8-1]
GeneIDi20917.
KEGGimmu:20917.
UCSCiuc009daa.3. mouse. [Q9Z2I8-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK167136 mRNA. Translation: BAE39282.1.
AK167339 mRNA. Translation: BAE39440.1.
AK172561 mRNA. Translation: BAE43068.1.
AK172633 mRNA. Translation: BAE43106.1.
BC030947 mRNA. Translation: AAH30947.1.
BC043312 mRNA. Translation: AAH43312.1.
BC054425 mRNA. Translation: AAH54425.1.
BC080781 mRNA. Translation: AAH80781.1.
AF058956 mRNA. Translation: AAC64399.1.
CCDSiCCDS20381.1. [Q9Z2I8-1]
RefSeqiNP_035637.2. NM_011507.3. [Q9Z2I8-1]
UniGeneiMm.371585.
Mm.426727.

3D structure databases

ProteinModelPortaliQ9Z2I8.
SMRiQ9Z2I8. Positions 39-430.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9Z2I8. 3 interactions.
MINTiMINT-1841325.
STRINGi10090.ENSMUSP00000078774.

PTM databases

iPTMnetiQ9Z2I8.
PhosphoSiteiQ9Z2I8.
SwissPalmiQ9Z2I8.

2D gel databases

REPRODUCTION-2DPAGEQ9Z2I8.

Proteomic databases

EPDiQ9Z2I8.
MaxQBiQ9Z2I8.
PaxDbiQ9Z2I8.
PeptideAtlasiQ9Z2I8.
PRIDEiQ9Z2I8.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000079847; ENSMUSP00000078774; ENSMUSG00000061838. [Q9Z2I8-2]
ENSMUST00000204224; ENSMUSP00000144827; ENSMUSG00000061838. [Q9Z2I8-1]
GeneIDi20917.
KEGGimmu:20917.
UCSCiuc009daa.3. mouse. [Q9Z2I8-1]

Organism-specific databases

CTDi8801.
MGIiMGI:1306824. Suclg2.

Phylogenomic databases

eggNOGiKOG2799. Eukaryota.
COG0045. LUCA.
GeneTreeiENSGT00390000010170.
HOVERGENiHBG055555.
InParanoidiQ9Z2I8.
KOiK01900.
OMAiDREHNGP.
OrthoDBiEOG7CZK66.
PhylomeDBiQ9Z2I8.
TreeFamiTF300624.

Enzyme and pathway databases

UniPathwayiUPA00223.
ReactomeiR-MMU-71403. Citric acid cycle (TCA cycle).

Miscellaneous databases

PROiQ9Z2I8.
SOURCEiSearch...

Gene expression databases

BgeeiQ9Z2I8.
ExpressionAtlasiQ9Z2I8. baseline.
GenevisibleiQ9Z2I8. MM.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.261. 1 hit.
InterProiIPR011761. ATP-grasp.
IPR013650. ATP-grasp_succ-CoA_synth-type.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR005811. CoA_ligase.
IPR017866. Succ-CoA_synthase_bsu_CS.
IPR005809. Succ_CoA_synthase_bsu.
IPR016102. Succinyl-CoA_synth-like.
[Graphical view]
PANTHERiPTHR11815. PTHR11815. 1 hit.
PfamiPF08442. ATP-grasp_2. 1 hit.
PF00549. Ligase_CoA. 1 hit.
[Graphical view]
PIRSFiPIRSF001554. SucCS_beta. 1 hit.
SUPFAMiSSF52210. SSF52210. 1 hit.
TIGRFAMsiTIGR01016. sucCoAbeta. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS01217. SUCCINYL_COA_LIG_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J and NOD.
    Tissue: Placenta and Spleen.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: C57BL/6J and FVB/N.
    Tissue: Kidney, Liver and Mammary tumor.
  3. "Genetic evidence for the expression of ATP- and GTP-specific succinyl-CoA synthetases in multicellular eucaryotes."
    Johnson J.D., Mehus J.G., Tews K., Milavetz B.I., Lambeth D.O.
    J. Biol. Chem. 273:27580-27586(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 30-433 (ISOFORM 1).
    Tissue: Heart.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-162 AND SER-217, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  5. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-67; LYS-79 AND LYS-339, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways."
    Rardin M.J., Newman J.C., Held J.M., Cusack M.P., Sorensen D.J., Li B., Schilling B., Mooney S.D., Kahn C.R., Verdin E., Gibson B.W.
    Proc. Natl. Acad. Sci. U.S.A. 110:6601-6606(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-67; LYS-74; LYS-112; LYS-133; LYS-140; LYS-201; LYS-219; LYS-228; LYS-272; LYS-348; LYS-387; LYS-407 AND LYS-424, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiSUCB2_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2I8
Secondary accession number(s): Q3T9B8
, Q3TJQ5, Q3TK63, Q66JT3, Q7TMY3, Q80VV1, Q8K2K9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 27, 2002
Last sequence update: January 10, 2006
Last modified: July 6, 2016
This is version 145 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.