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Protein

Mitochondrial proton/calcium exchanger protein

Gene

Letm1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mitochondrial proton/calcium antiporter that mediates proton-dependent calcium efflux from mitochondrion (PubMed:27669901). Crucial for the maintenance of mitochondrial tubular networks and for the assembly of the supercomplexes of the respiratory chain (By similarity). Required for the maintenance of the tubular shape and cristae organization (By similarity). In contrast to SLC8B1/NCLX, does not constitute the major factor for mitochondrial calcium extrusion (By similarity).By similarity1 Publication

Enzyme regulationi

Inhibited by ruthenium red or its derivative Ru360.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi675 – 687Sequence analysisAdd BLAST13

GO - Molecular functioni

  • calcium:proton antiporter activity Source: UniProtKB
  • metal ion binding Source: UniProtKB-KW
  • ribosome binding Source: InterPro

GO - Biological processi

  • calcium export from the mitochondrion Source: UniProtKB
  • cellular metal ion homeostasis Source: GO_Central
  • cristae formation Source: MGI
  • mitochondrial calcium ion homeostasis Source: UniProtKB
  • mitochondrial calcium ion transmembrane transport Source: UniProtKB
  • protein hexamerization Source: UniProtKB
  • protein homooligomerization Source: UniProtKB

Keywordsi

Biological processAntiport, Calcium transport, Ion transport, Transport
LigandCalcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial proton/calcium exchanger proteinCurated
Alternative name(s):
Leucine zipper-EF-hand-containing transmembrane protein 1
Gene namesi
Name:Letm1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1932557 Letm1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini115 – 207Mitochondrial intermembrane1 PublicationAdd BLAST93
Transmembranei208 – 228HelicalSequence analysisAdd BLAST21
Topological domaini229 – 738Mitochondrial matrix1 PublicationAdd BLAST510

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi204D → N: Does not affect mitochondrial localization and ability to mediate calcium efflux from mitochondrion. 1 Publication1
Mutagenesisi221E → Q: Does not affect mitochondrial localization but abolishes ability to mediate calcium efflux from mitochondrion. 1 Publication1
Mutagenesisi246 – 247EE → AA: Does not affect mitochondrial localization and ability to mediate calcium efflux from mitochondrion. 1 Publication2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 114MitochondrionSequence analysisAdd BLAST114
ChainiPRO_0000017695115 – 738Mitochondrial proton/calcium exchanger proteinAdd BLAST624

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei596N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9Z2I0
MaxQBiQ9Z2I0
PaxDbiQ9Z2I0
PeptideAtlasiQ9Z2I0
PRIDEiQ9Z2I0

PTM databases

iPTMnetiQ9Z2I0
PhosphoSitePlusiQ9Z2I0
SwissPalmiQ9Z2I0

Expressioni

Gene expression databases

BgeeiENSMUSG00000005299
ExpressionAtlasiQ9Z2I0 baseline and differential
GenevisibleiQ9Z2I0 MM

Interactioni

Subunit structurei

Homohexamer (PubMed:27669901). Interacts with BCS1L (By similarity).By similarity1 Publication

Protein-protein interaction databases

BioGridi207944, 1 interactor
IntActiQ9Z2I0, 5 interactors
MINTiQ9Z2I0
STRINGi10090.ENSMUSP00000005431

Structurei

3D structure databases

ProteinModelPortaliQ9Z2I0
SMRiQ9Z2I0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini251 – 536Letm1 RBDPROSITE-ProRule annotationAdd BLAST286
Domaini662 – 697EF-handAdd BLAST36

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili115 – 135Sequence analysisAdd BLAST21
Coiled coili247 – 283Sequence analysisAdd BLAST37
Coiled coili445 – 492Sequence analysisAdd BLAST48
Coiled coili537 – 626Sequence analysisAdd BLAST90
Coiled coili707 – 738Sequence analysisAdd BLAST32

Sequence similaritiesi

Belongs to the LETM1 family.Curated

Keywords - Domaini

Coiled coil, Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1043 Eukaryota
ENOG410XRSP LUCA
GeneTreeiENSGT00390000011225
HOGENOMiHOG000290246
HOVERGENiHBG052322
InParanoidiQ9Z2I0
KOiK17800
OMAiARMTTEQ
OrthoDBiEOG091G034T
PhylomeDBiQ9Z2I0
TreeFamiTF316321

Family and domain databases

InterProiView protein in InterPro
IPR011992 EF-hand-dom_pair
IPR018247 EF_Hand_1_Ca_BS
IPR011685 LETM1
IPR033122 LETM1_RBD
PfamiView protein in Pfam
PF07766 LETM1, 1 hit
SUPFAMiSSF47473 SSF47473, 1 hit
PROSITEiView protein in PROSITE
PS00018 EF_HAND_1, 1 hit
PS51758 LETM1_RBD, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z2I0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASILLRSCR GRGPARLAPP RAASPRGSLR DRACLSCTRT LGLTSRESVL
60 70 80 90 100
SRCCTPAHPV YLCFKGEPLS CWTQRPECQG TAARTTWTPA SARLVVTGPQ
110 120 130 140 150
YLPVRGWHSS SPLGEDSVIE KSLKSLKDKN KKLEEGGPVY SPPAQVVVRK
160 170 180 190 200
SLGQKVLDEL RHYYHGFRLL WIDTKIAARM LWRILNGHTL TRRERRQFLR
210 220 230 240 250
ICADLFRLVP FLVFVVVPFM EFLLPVVVKL FPNMLPSTFE TQSIKEERLK
260 270 280 290 300
KELRVKLELA KFLQDTIEEM ALKNKAAKGN ATKDFSAFFQ KIRETGERPS
310 320 330 340 350
NEEIMRFSKL FEDELTLDNL TRPQLVALCK LLELQSIGTN NFLRFQLTMR
360 370 380 390 400
LRSIKADDKL ISEEGVDSLT VKELQAACRA RGMRALGVTE DRLKGQLKQW
410 420 430 440 450
LDLHLHHEIP TSLLILSRAM YLPDTLSPAD QLKSTLQTLP EIVAKEAQVK
460 470 480 490 500
VAEVEGEKVD NKAKLEATLQ EEAAIQQEHL EELKRASEAV KDIQPEVAEA
510 520 530 540 550
TLPGRPGPEP QPPVDDVILP SEVLTDTAPV LEGLKGEEIT KEEIDILSDA
560 570 580 590 600
CSKLQEQKKS LTKEKEELEL LKEDVQDYSE DLQEIKKELS KTGEEKYIEE
610 620 630 640 650
SAASKRLSKR VQQMIGQIDG LITQLETTQQ DGKLGPSQST PTGESVISIT
660 670 680 690 700
ELISAMKQIK HIPEHKLISL TSALDDNKDG NINIDDLVKV IDLVNKEDVQ
710 720 730
ISTTQVAEIV ATLEKEEKIE EKEKAKEKAE KEAAEVKN
Length:738
Mass (Da):82,989
Last modified:May 1, 1999 - v1
Checksum:i5074CE6309940729
GO

Sequence cautioni

The sequence AAH23862 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH46326 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated
The sequence AAH55865 differs from that shown. Contaminating sequence. Potential poly-A sequence.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti271A → P in AAH23862 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061026 mRNA Translation: AAD13139.1
BC023862 mRNA Translation: AAH23862.1 Sequence problems.
BC046326 mRNA Translation: AAH46326.1 Sequence problems.
BC055865 mRNA Translation: AAH55865.1 Sequence problems.
BC061115 mRNA Translation: AAH61115.1
AB041582 mRNA Translation: BAB93544.1
CCDSiCCDS19207.1
RefSeqiNP_062668.1, NM_019694.1
UniGeneiMm.260538

Genome annotation databases

EnsembliENSMUST00000005431; ENSMUSP00000005431; ENSMUSG00000005299
GeneIDi56384
KEGGimmu:56384
UCSCiuc008xbi.1 mouse

Similar proteinsi

Entry informationi

Entry nameiLETM1_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2I0
Secondary accession number(s): Q5PQC5
, Q7TMK8, Q80ZX6, Q8CGJ3, Q8K5E5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 12, 2005
Last sequence update: May 1, 1999
Last modified: April 25, 2018
This is version 134 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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