Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Leucine-rich repeat-containing G-protein coupled receptor 4

Gene

Lgr4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and is involved in the formation of various organs. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Its function as activator of the Wnt signaling pathway is required for the development of various organs, including liver, kidney, intestine, bone, reproductive tract and eye. May also act as a receptor for norrin (NDP), such results however require additional confirmation in vivo. Required during spermatogenesis to activate the Wnt signaling pathway in peritubular myoid cells. Required for the maintenance of intestinal stem cells and Paneth cell differentiation in postnatal intestinal crypts. Acts as a regulator of bone formation and remodeling. Involved in kidney development; required for maintaining the ureteric bud in an undifferentiated state. Involved in the development of the anterior segment of the eye. Required during erythropoiesis. Also acts as a negative regulator of innate immunity by inhibiting TLR2/TLR4 associated pattern-recognition and proinflammatory cytokine production. Plays an important role in regulating the circadian rhythms of plasma lipids, partially through regulating the rhythmic expression of MTTP.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, G-protein coupled receptor, Receptor, Transducer

Keywords - Biological processi

Biological rhythms, Differentiation, Immunity, Innate immunity, Spermatogenesis, Wnt signaling pathway

Enzyme and pathway databases

ReactomeiR-RNO-4641263. Regulation of FZD by ubiquitination.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine-rich repeat-containing G-protein coupled receptor 4
Alternative name(s):
G-protein coupled receptor 48
Gene namesi
Name:Lgr4
Synonyms:Gpr48
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi628615. Lgr4.

Subcellular locationi

  • Cell membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 544520ExtracellularSequence analysisAdd
BLAST
Transmembranei545 – 56521Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini566 – 57510CytoplasmicSequence analysis
Transmembranei576 – 59621Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini597 – 61923ExtracellularSequence analysisAdd
BLAST
Transmembranei620 – 64021Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini641 – 66121CytoplasmicSequence analysisAdd
BLAST
Transmembranei662 – 68221Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini683 – 70321ExtracellularSequence analysisAdd
BLAST
Transmembranei704 – 72421Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini725 – 75632CytoplasmicSequence analysisAdd
BLAST
Transmembranei757 – 77721Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini778 – 7836ExtracellularSequence analysis
Transmembranei784 – 80421Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini805 – 951147CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence analysisAdd
BLAST
Chaini25 – 951927Leucine-rich repeat-containing G-protein coupled receptor 4PRO_0000012793Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi29 ↔ 35PROSITE-ProRule annotation
Disulfide bondi33 ↔ 43PROSITE-ProRule annotation
Glycosylationi68 – 681N-linked (GlcNAc...)Sequence analysis
Glycosylationi188 – 1881N-linked (GlcNAc...)Sequence analysis
Glycosylationi199 – 1991N-linked (GlcNAc...)Sequence analysis
Glycosylationi294 – 2941N-linked (GlcNAc...)Sequence analysis
Glycosylationi314 – 3141N-linked (GlcNAc...)Sequence analysis
Disulfide bondi339 ↔ 364PROSITE-ProRule annotation
Disulfide bondi470 ↔ 522PROSITE-ProRule annotation
Disulfide bondi471 ↔ 476PROSITE-ProRule annotation
Glycosylationi505 – 5051N-linked (GlcNAc...)Sequence analysis
Disulfide bondi618 ↔ 693PROSITE-ProRule annotation
Modified residuei920 – 9201PhosphoserineBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9Z2H4.

PTM databases

PhosphoSiteiQ9Z2H4.

Expressioni

Gene expression databases

BgeeiENSRNOG00000005715.
GenevisibleiQ9Z2H4. RN.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000007681.

Structurei

3D structure databases

ProteinModelPortaliQ9Z2H4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 5733LRRNTAdd
BLAST
Repeati58 – 7922LRR 1Add
BLAST
Repeati82 – 10322LRR 2Add
BLAST
Repeati106 – 12722LRR 3Add
BLAST
Repeati130 – 15122LRR 4Add
BLAST
Repeati154 – 17724LRR 5Add
BLAST
Repeati178 – 19922LRR 6Add
BLAST
Repeati202 – 22322LRR 7Add
BLAST
Repeati226 – 24722LRR 8Add
BLAST
Repeati249 – 27022LRR 9Add
BLAST
Repeati273 – 29422LRR 10Add
BLAST
Repeati320 – 34122LRR 11Add
BLAST
Repeati344 – 36522LRR 12Add
BLAST
Repeati366 – 38722LRR 13Add
BLAST
Repeati390 – 41122LRR 14Add
BLAST
Repeati414 – 43522LRR 15Add
BLAST

Sequence similaritiesi

Belongs to the G-protein coupled receptor 1 family.PROSITE-ProRule annotation
Contains 15 LRR (leucine-rich) repeats.Curated
Contains 1 LRRNT domain.Curated

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
KOG2087. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00760000119088.
HOGENOMiHOG000231829.
HOVERGENiHBG031675.
InParanoidiQ9Z2H4.
KOiK04309.
OMAiCAAPCSC.
OrthoDBiEOG091G0QA0.
TreeFamiTF316814.

Family and domain databases

Gene3Di3.80.10.10. 4 hits.
InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR002131. Gphrmn_rcpt_fam.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000372. LRRNT.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
PF13855. LRR_8. 4 hits.
PF01462. LRRNT. 1 hit.
[Graphical view]
PRINTSiPR00373. GLYCHORMONER.
PR00237. GPCRRHODOPSN.
SMARTiSM00369. LRR_TYP. 15 hits.
SM00013. LRRNT. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 2 hits.
PROSITEiPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
PS51450. LRR. 15 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z2H4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPGPLRLLCF FALGLLGSAG PSGAAPPLCA APCSCDGDRR VDCSGKGLTA
60 70 80 90 100
VPEGLSAFTQ ALDISMNNIT QLPEDAFKSF PFLEELQLAG NDLSFIHPKA
110 120 130 140 150
LSGLKELKVL TLQNNQLRTV PSEAIHGLSA LQSLRLDANH ITSVPEDSFE
160 170 180 190 200
GLVQLRHLWL DDNSLTEVPV RPLSNLPTLQ ALTLALNNIS SIPDFAFTNL
210 220 230 240 250
SSLVVLHLHN NKIKSLSQHC FDGLDNLETL DLNYNNLDEF PQAIKALPSL
260 270 280 290 300
KELGFHSNSI SVIPDGAFGG NPLLRTIHLY DNPLSFVGNS AFHNLSDLHS
310 320 330 340 350
LVIRGASLVQ WFPNLTGTVH LESLTLTGTK ISSIPDDLCQ NQKMLRTLDL
360 370 380 390 400
SYNNIRDLPS FNGCRALEEI SLQRNQISLI KENTFQGLTS LRILDLSRNL
410 420 430 440 450
IREIHSGAFA KLGTITNLDV SFNELTSFPT EGLNGLNQLK LVGNFKLKDA
460 470 480 490 500
LAARDFANLR SLSVPYAYQC CAFWGCDSYA NLNTEDNSPQ EHSVTKEKGA
510 520 530 540 550
TDAANVTSTA ENEEHSQIII HCTPSTGAFK PCEYLLGSWM IRLTVWFIFL
560 570 580 590 600
VALLFNLLVI LTVFASCSSL PASKLFIGLI SVSNLLMGIY TGILTFLDAV
610 620 630 640 650
SWGRFAEFGI WWETGSGCKV AGSLAVFSSE SAVFLLTLAA VERSVFAKDL
660 670 680 690 700
MKHGKSSHLR QFQVAALLAL LGAAVAGCFP LFHGGQYSAS PLCLPFPTGE
710 720 730 740 750
TPSLGFTVTL VLLNSLAFLL MAIIYTKLYC NLEKEDLSEN SQSSVIKHVA
760 770 780 790 800
WLIFTNCIFF CPVAFFSFAP LITAISISPE IMKSVTLIFF PLPACLNPVL
810 820 830 840 850
YVFFNPKFKE DWKLLKRRVT RKHGSVSVSI SSQGGCGEQD FYYDCGMYSH
860 870 880 890 900
LQGNLTVCDC CESFLLTKPV SCKHLIKSHS CPVLTAASCQ RPEAYWSDCG
910 920 930 940 950
TQSAHSDYAD EEDSFVSDSS DQVQACGRAC FYQSRGFPLV RYAYNLQRVR

D
Length:951
Mass (Da):104,241
Last modified:June 26, 2013 - v2
Checksum:i457798D5408DC403
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti6 – 61R → G in AAC77910 (PubMed:9849958).Curated
Sequence conflicti11 – 111F → L in AAC77910 (PubMed:9849958).Curated
Sequence conflicti95 – 951F → L in AAC77910 (PubMed:9849958).Curated
Sequence conflicti236 – 2361N → Y in AAC77910 (PubMed:9849958).Curated
Sequence conflicti300 – 3001S → C in AAC77910 (PubMed:9849958).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061443 mRNA. Translation: AAC77910.1.
AABR06025467 Genomic DNA. No translation available.
AABR06025468 Genomic DNA. No translation available.
AABR06025469 Genomic DNA. No translation available.
AABR06025470 Genomic DNA. No translation available.
AABR06025471 Genomic DNA. No translation available.
CH473949 Genomic DNA. Translation: EDL79759.1.
RefSeqiNP_775450.1. NM_173328.1.
UniGeneiRn.162671.

Genome annotation databases

EnsembliENSRNOT00000007681; ENSRNOP00000007681; ENSRNOG00000005715.
GeneIDi286994.
KEGGirno:286994.
UCSCiRGD:628615. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061443 mRNA. Translation: AAC77910.1.
AABR06025467 Genomic DNA. No translation available.
AABR06025468 Genomic DNA. No translation available.
AABR06025469 Genomic DNA. No translation available.
AABR06025470 Genomic DNA. No translation available.
AABR06025471 Genomic DNA. No translation available.
CH473949 Genomic DNA. Translation: EDL79759.1.
RefSeqiNP_775450.1. NM_173328.1.
UniGeneiRn.162671.

3D structure databases

ProteinModelPortaliQ9Z2H4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000007681.

Protein family/group databases

GPCRDBiSearch...

PTM databases

PhosphoSiteiQ9Z2H4.

Proteomic databases

PaxDbiQ9Z2H4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000007681; ENSRNOP00000007681; ENSRNOG00000005715.
GeneIDi286994.
KEGGirno:286994.
UCSCiRGD:628615. rat.

Organism-specific databases

CTDi55366.
RGDi628615. Lgr4.

Phylogenomic databases

eggNOGiKOG0619. Eukaryota.
KOG2087. Eukaryota.
COG4886. LUCA.
GeneTreeiENSGT00760000119088.
HOGENOMiHOG000231829.
HOVERGENiHBG031675.
InParanoidiQ9Z2H4.
KOiK04309.
OMAiCAAPCSC.
OrthoDBiEOG091G0QA0.
TreeFamiTF316814.

Enzyme and pathway databases

ReactomeiR-RNO-4641263. Regulation of FZD by ubiquitination.

Miscellaneous databases

PROiQ9Z2H4.

Gene expression databases

BgeeiENSRNOG00000005715.
GenevisibleiQ9Z2H4. RN.

Family and domain databases

Gene3Di3.80.10.10. 4 hits.
InterProiIPR000276. GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_7TM.
IPR002131. Gphrmn_rcpt_fam.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR000372. LRRNT.
[Graphical view]
PfamiPF00001. 7tm_1. 1 hit.
PF13855. LRR_8. 4 hits.
PF01462. LRRNT. 1 hit.
[Graphical view]
PRINTSiPR00373. GLYCHORMONER.
PR00237. GPCRRHODOPSN.
SMARTiSM00369. LRR_TYP. 15 hits.
SM00013. LRRNT. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 2 hits.
PROSITEiPS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
PS51450. LRR. 15 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLGR4_RAT
AccessioniPrimary (citable) accession number: Q9Z2H4
Secondary accession number(s): G3V6R1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2002
Last sequence update: June 26, 2013
Last modified: September 7, 2016
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. 7-transmembrane G-linked receptors
    List of 7-transmembrane G-linked receptor entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.