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Protein

Oxidoreductase HTATIP2

Gene

Htatip2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Oxidoreductase required for tumor suppression. NAPDH-bound form inhibits nuclear import by competing with nuclear import substrates for binding to a subset of nuclear transport receptors. May act as a redox sensor linked to transcription through regulation of nuclear import.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei131 – 1311SubstrateBy similarity
Active sitei143 – 1431Proton acceptorBy similarity
Active sitei147 – 1471By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi19 – 5234NADPBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Oxidoreductase

Keywords - Biological processi

Angiogenesis, Apoptosis, Differentiation

Keywords - Ligandi

NADP

Names & Taxonomyi

Protein namesi
Recommended name:
Oxidoreductase HTATIP2 (EC:1.1.1.-)
Gene namesi
Name:Htatip2Imported
Synonyms:Cc31 Publication, Tip301 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1859271. Htatip2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • membrane Source: MGI
  • nuclear envelope Source: UniProtKB
  • nucleoplasm Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Disruption phenotypei

Mice are haploinsufficient for tumor suppression. 50% develop tumors within their second year. 30% of the tumors are hepatocellular carcinomas.1 Publication

Keywords - Diseasei

Tumor suppressor

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 242241Oxidoreductase HTATIP2PRO_0000072545Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9Z2G9.
MaxQBiQ9Z2G9.
PaxDbiQ9Z2G9.
PRIDEiQ9Z2G9.

PTM databases

iPTMnetiQ9Z2G9.
PhosphoSiteiQ9Z2G9.
SwissPalmiQ9Z2G9.

Expressioni

Gene expression databases

CleanExiMM_HTATIP2.
ExpressionAtlasiQ9Z2G9. baseline and differential.
GenevisibleiQ9Z2G9. MM.

Interactioni

Subunit structurei

Monomer. Binds nuclear transport receptors XPO4, IPO5/RANBP5, IPO7, IPO9 and KPNB1 as well as GCN1L1/GCN1 and LRPPRC probably through their HEAT repeats. Binds NCOA5/CIA (By similarity).By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000082374.

Structurei

Secondary structure

1
242
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 1610Combined sources
Beta strandi20 – 245Combined sources
Helixi29 – 4113Combined sources
Beta strandi45 – 539Combined sources
Beta strandi66 – 694Combined sources
Helixi72 – 8211Combined sources
Beta strandi86 – 905Combined sources
Helixi101 – 1088Combined sources
Helixi110 – 12112Combined sources
Beta strandi126 – 1305Combined sources
Helixi142 – 15615Combined sources
Beta strandi160 – 1667Combined sources
Beta strandi168 – 1714Combined sources
Helixi195 – 1995Combined sources
Beta strandi200 – 2023Combined sources
Helixi203 – 21513Combined sources
Beta strandi219 – 2268Combined sources
Helixi227 – 2337Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FMUX-ray2.30A7-242[»]
ProteinModelPortaliQ9Z2G9.
SMRiQ9Z2G9. Positions 5-235.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9Z2G9.

Family & Domainsi

Phylogenomic databases

eggNOGiKOG4039. Eukaryota.
COG0702. LUCA.
GeneTreeiENSGT00390000008184.
HOVERGENiHBG052833.
InParanoidiQ9Z2G9.
KOiK17290.
OMAiDRYTIFR.
OrthoDBiEOG7GJ6GJ.
TreeFamiTF312849.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000534. Semialdehyde_DH_NAD-bd.
[Graphical view]
PfamiPF13460. NAD_binding_10. 1 hit.
[Graphical view]
SMARTiSM00859. Semialdhyde_dh. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q9Z2G9-1) [UniParc]FASTAAdd to basket

Also known as: TIP301 Publication

, CC31 Publication

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADKEALPKL REDFKMQNKS VFILGASGET GKVLLKEILG QNLFSKVTLI
60 70 80 90 100
GRRKLTFEEE AYKNVNQEVV DFEKLDVYAS AFQGHDVGFC CLGTTRSKAG
110 120 130 140 150
AEGFVRVDRD YVLKSAELAK AGGCKHFNLL SSRGADKSSS FLYLQVKGEV
160 170 180 190 200
EAKVEELKFD RLSVFRPGVL LCDRQESRPG EWLARKFFGS LPDSWASGYA
210 220 230 240
VPVVTVVRAM LNNLVSPSSG QMELLENKAI LHLGKDRDVP KL
Length:242
Mass (Da):26,870
Last modified:October 3, 2012 - v3
Checksum:i034F02137D3BF22A
GO
Isoform 2Curated (identifier: Q9Z2G9-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     102-105: EGFV → VSKK
     106-242: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:105
Mass (Da):11,661
Checksum:i8DF855A01EEF6BEB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti8 – 81P → R in AAC69617 (PubMed:14695192).Curated
Sequence conflicti10 – 101L → P in BAB29825 (PubMed:16141072).Curated
Sequence conflicti27 – 271S → T in BAB29825 (PubMed:16141072).Curated
Sequence conflicti39 – 391L → V in AAC69617 (PubMed:14695192).Curated
Sequence conflicti77 – 771V → G in AAN84531 (Ref. 2) Curated
Sequence conflicti213 – 2131N → S in AAC69617 (PubMed:14695192).Curated
Sequence conflicti235 – 2351K → I in BAB25792 (PubMed:16141072).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei102 – 1054EGFV → VSKK in isoform 2. 1 PublicationVSP_051866
Alternative sequencei106 – 242137Missing in isoform 2. 1 PublicationVSP_051867Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061972 mRNA. Translation: AAC69617.1.
AY151050 Genomic DNA. Translation: AAN84531.1.
AK008630 mRNA. Translation: BAB25792.1.
AK015389 mRNA. Translation: BAB29825.1.
AK144837 mRNA. Translation: BAE26091.1.
AC124775 Genomic DNA. No translation available.
CH466603 Genomic DNA. Translation: EDL22975.1.
CH466603 Genomic DNA. Translation: EDL22976.1.
CH466603 Genomic DNA. Translation: EDL22977.1.
BC004083 mRNA. Translation: AAH04083.1.
BC017372 mRNA. Translation: AAH17372.1.
CCDSiCCDS52260.1. [Q9Z2G9-1]
RefSeqiNP_001139521.1. NM_001146049.1. [Q9Z2G9-1]
NP_001139522.1. NM_001146050.1. [Q9Z2G9-1]
NP_001139524.1. NM_001146052.1. [Q9Z2G9-1]
NP_001139525.1. NM_001146053.1. [Q9Z2G9-1]
UniGeneiMm.20801.

Genome annotation databases

EnsembliENSMUST00000085272; ENSMUSP00000082374; ENSMUSG00000039745. [Q9Z2G9-1]
GeneIDi53415.
KEGGimmu:53415.
UCSCiuc009hbo.2. mouse. [Q9Z2G9-2]
uc009hbq.2. mouse. [Q9Z2G9-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061972 mRNA. Translation: AAC69617.1.
AY151050 Genomic DNA. Translation: AAN84531.1.
AK008630 mRNA. Translation: BAB25792.1.
AK015389 mRNA. Translation: BAB29825.1.
AK144837 mRNA. Translation: BAE26091.1.
AC124775 Genomic DNA. No translation available.
CH466603 Genomic DNA. Translation: EDL22975.1.
CH466603 Genomic DNA. Translation: EDL22976.1.
CH466603 Genomic DNA. Translation: EDL22977.1.
BC004083 mRNA. Translation: AAH04083.1.
BC017372 mRNA. Translation: AAH17372.1.
CCDSiCCDS52260.1. [Q9Z2G9-1]
RefSeqiNP_001139521.1. NM_001146049.1. [Q9Z2G9-1]
NP_001139522.1. NM_001146050.1. [Q9Z2G9-1]
NP_001139524.1. NM_001146052.1. [Q9Z2G9-1]
NP_001139525.1. NM_001146053.1. [Q9Z2G9-1]
UniGeneiMm.20801.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2FMUX-ray2.30A7-242[»]
ProteinModelPortaliQ9Z2G9.
SMRiQ9Z2G9. Positions 5-235.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000082374.

PTM databases

iPTMnetiQ9Z2G9.
PhosphoSiteiQ9Z2G9.
SwissPalmiQ9Z2G9.

Proteomic databases

EPDiQ9Z2G9.
MaxQBiQ9Z2G9.
PaxDbiQ9Z2G9.
PRIDEiQ9Z2G9.

Protocols and materials databases

DNASUi53415.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000085272; ENSMUSP00000082374; ENSMUSG00000039745. [Q9Z2G9-1]
GeneIDi53415.
KEGGimmu:53415.
UCSCiuc009hbo.2. mouse. [Q9Z2G9-2]
uc009hbq.2. mouse. [Q9Z2G9-1]

Organism-specific databases

CTDi10553.
MGIiMGI:1859271. Htatip2.

Phylogenomic databases

eggNOGiKOG4039. Eukaryota.
COG0702. LUCA.
GeneTreeiENSGT00390000008184.
HOVERGENiHBG052833.
InParanoidiQ9Z2G9.
KOiK17290.
OMAiDRYTIFR.
OrthoDBiEOG7GJ6GJ.
TreeFamiTF312849.

Miscellaneous databases

EvolutionaryTraceiQ9Z2G9.
NextBioi310233.
PROiQ9Z2G9.
SOURCEiSearch...

Gene expression databases

CleanExiMM_HTATIP2.
ExpressionAtlasiQ9Z2G9. baseline and differential.
GenevisibleiQ9Z2G9. MM.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR000534. Semialdehyde_DH_NAD-bd.
[Graphical view]
PfamiPF13460. NAD_binding_10. 1 hit.
[Graphical view]
SMARTiSM00859. Semialdhyde_dh. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "TIP30 deficiency increases susceptibility to tumorigenesis."
    Ito M., Jiang C., Krumm K., Zhang X., Pecha J., Zhao J., Guo Y., Roeder R.G., Xiao H.
    Cancer Res. 63:8763-8767(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, DISRUPTION PHENOTYPE.
  2. "Genomic organization, chromosomal mapping, and expression analysis of the murine Prmt3 and Htatip2 genes."
    Hauser L.J., Webb L.S., Dhar M.S., Mural R.M., Larimer F.W., Johnson D.K.
    Submitted (SEP-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 129/SvJImported.
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Strain: C57BL/6J.
    Tissue: Lung, Stomach and Testis.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  5. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Czech II and FVB/N.
    Tissue: Mammary tumor.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brown adipose tissue, Heart, Liver, Lung, Spleen and Testis.
  8. "Crystal structure of Tat-interacting protein 30 kDa (HIV-1 Tat-interactive protein 2, 30 kDa homolog) (human) (16924205) from Mus musculus at 2.30 A resolution."
    Joint center for structural genomics (JCSG)
    Submitted (FEB-2006) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS) OF 7-242.

Entry informationi

Entry nameiHTAI2_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2G9
Secondary accession number(s): Q810Y5
, Q99KN6, Q9D5F8, Q9D804
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 3, 2012
Last modified: May 11, 2016
This is version 103 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.