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Protein

G protein-coupled receptor kinase 7

Gene

GRK7

Organism
Spermophilus tridecemlineatus (Thirteen-lined ground squirrel) (Ictidomys tridecemlineatus)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Retina-specific kinase involved in the shutoff of the photoresponse and adaptation to changing light conditions via cone opsin phosphorylation, including rhodopsin (RHO).By similarity

Catalytic activityi

ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.
ATP + [rhodopsin] = ADP + [rhodopsin] phosphate.By similarity

Enzyme regulationi

Inhibited by phosphorylation of Ser-34.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei215 – 2151ATPPROSITE-ProRule annotation
Active sitei311 – 3111Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi192 – 2009ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. G-protein coupled receptor kinase activity Source: UniProtKB-EC
  3. rhodopsin kinase activity Source: UniProtKB

GO - Biological processi

  1. signal transduction Source: InterPro
  2. termination of G-protein coupled receptor signaling pathway Source: InterPro
  3. visual perception Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
G protein-coupled receptor kinase 7 (EC:2.7.11.14By similarity, EC:2.7.11.16)
Alternative name(s):
G protein-coupled receptor kinase GRK7
Gene namesi
Name:GRK7
OrganismiSpermophilus tridecemlineatus (Thirteen-lined ground squirrel) (Ictidomys tridecemlineatus)
Taxonomic identifieri43179 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiSciuridaeXerinaeMarmotiniIctidomys
ProteomesiUP000005215: Unplaced

Subcellular locationi

Membrane By similarity; Lipid-anchor By similarity

GO - Cellular componenti

  1. membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 545545G protein-coupled receptor kinase 7PRO_0000024336Add
BLAST
Propeptidei546 – 5483Removed in mature formBy similarityPRO_0000024337

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei34 – 341Phosphoserine; by PKABy similarity
Modified residuei545 – 5451Cysteine methyl esterSequence Analysis
Lipidationi545 – 5451S-geranylgeranyl cysteineSequence Analysis

Post-translational modificationi

Autophosphorylated. Phosphorylation at Ser-34 is regulated by light and activated by cAMP (By similarity).By similarity

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Expressioni

Tissue specificityi

Retina. Cones and rod.1 Publication

Interactioni

Subunit structurei

Interacts (when prenylated) with PDE6D; this promotes release from membranes.By similarity

Structurei

3D structure databases

ProteinModelPortaliQ9Z2G7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini54 – 171118RGSPROSITE-ProRule annotationAdd
BLAST
Domaini186 – 449264Protein kinasePROSITE-ProRule annotationAdd
BLAST
Domaini450 – 51566AGC-kinase C-terminalAdd
BLAST

Sequence similaritiesi

Contains 1 AGC-kinase C-terminal domain.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 RGS domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG004532.
InParanoidiQ9Z2G7.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. RGS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR00717. GPCRKINASE.
SMARTiSM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9Z2G7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDMGGLDNLI ANTAYLQARK TDSDSRELQR RRRSLALPGP QGCAELRQSL
60 70 80 90 100
SPHFHSLCEQ QPIGRRLFRD FLATVPKYSQ AVAFLEDVQN WELAEEGPAK
110 120 130 140 150
TSTLQQLAAT CARDPGPQSF LSQDLATKCR AASTDEERKT LVEQAKAETM
160 170 180 190 200
SFLQEQPFQD FLASPFYDRF LQWKLFEMQP VSDKYFTEFR VLGKGGFGEV
210 220 230 240 250
CAVQVRNTGK MYACKKLDKK RLKKKGGEKM ALLEKEILEK VNSPFIVSLA
260 270 280 290 300
YAFESKTHLC LVMSLMNGGD LKFHIYNVGT RGLAMSRVIF YTAQMTCGVL
310 320 330 340 350
HLHGLGIVYR DLKPENVLLD DLGNCRLSDL GLAVEVQDDK PITQRAGTNG
360 370 380 390 400
YMAPEILMDK ASYSYPVDWF AMGCSIYEMV AGRTPFKDFK EKVSKEDLKE
410 420 430 440 450
RTMKDEVAFH HENFTEETKD ICRLFLAKKP EQRLGSREKA DDPRKHPFFQ
460 470 480 490 500
TVNFPRLEAG LVEPPFVPDP SVVYAKDVDE IDDFSEVRGV EFDDKDKQFF
510 520 530 540
QRFSTGAVPV AWQEEIIETG LFEELNDPNR PSGDGKGDSS KSGVCLLL
Length:548
Mass (Da):61,993
Last modified:May 1, 1999 - v1
Checksum:i092C5BFA5A42A200
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF063016 mRNA. Translation: AAC95001.1.
RefSeqiNP_001269199.1. NM_001282270.1.

Genome annotation databases

GeneIDi101975039.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF063016 mRNA. Translation: AAC95001.1.
RefSeqiNP_001269199.1. NM_001282270.1.

3D structure databases

ProteinModelPortaliQ9Z2G7.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi101975039.

Organism-specific databases

CTDi131890.

Phylogenomic databases

HOVERGENiHBG004532.
InParanoidiQ9Z2G7.

Family and domain databases

InterProiIPR000961. AGC-kinase_C.
IPR000239. GPCR_kinase.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR016137. RGS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
PF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR00717. GPCRKINASE.
SMARTiSM00315. RGS. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50132. RGS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The cloning of GRK7, a candidate cone opsin kinase, from cone- and rod-dominant mammalian retinas."
    Weiss E.R., Raman D., Shirakawa S., Ducceschi M.S., Bertram P.T., Wong F., Kraft T.W., Osawa S.
    Mol. Vis. 4:27-27(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AUTOPHOSPHORYLATION.
    Tissue: Retina.

Entry informationi

Entry nameiGRK7_SPETR
AccessioniPrimary (citable) accession number: Q9Z2G7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: May 1, 1999
Last modified: January 7, 2015
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Although the protein is present in a diversity of vertebrates ranging from bony fish to mammals, the mouse and rat orthologous proteins do not exist.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.