G protein-coupled receptor kinase 7 precursor - Spermophilus tridecemlineatus (Thirteen-lined ground squirrel)

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Q9Z2G7 (GRK7_SPETR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 79. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
G protein-coupled receptor kinase 7
EC=2.7.11.14
EC=2.7.11.16

Alternative name(s):
G protein-coupled receptor kinase GRK7

Gene names

Name:

GRK7

Organism

Spermophilus tridecemlineatus (Thirteen-lined ground squirrel) (Ictidomys tridecemlineatus) [Reference proteome]

Taxonomic identifier

43179 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Sciuridae › Xerinae › Marmotini › Spermophilus › Ictidomys

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Retina-specific kinase involved in the shutoff of the photoresponse and adaptation to changing light conditions via cone opsin phosphorylation, including rhodopsin (RHO) By similarity.
Catalytic activity	ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate. ATP + [rhodopsin] = ADP + [rhodopsin] phosphate.
Enzyme regulation	Inhibited by phosphorylation of Ser-34 By similarity.
Subcellular location	Membrane; Lipid-anchor By similarity.
Tissue specificity	Retina. Cones and rod. Ref.1
Post-translational modification	Autophosphorylated. Phosphorylation at Ser-34 is regulated by light and activated by cAMP By similarity. Ref.1
Miscellaneous	Although the protein is present in a diversity of vertebrates ranging from bony fish to mammals, the mouse and rat orthologous proteins do not exist.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain. Contains 1 RGS domain.

Ontologies

Keywords
Biological process	Sensory transduction Vision
Cellular component	Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Lipoprotein Methylation Phosphoprotein Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	signal transduction Inferred from electronic annotation. Source: InterPro termination of G-protein coupled receptor signaling pathway Inferred from electronic annotation. Source: InterPro visual perception Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW G-protein coupled receptor kinase activity Inferred from electronic annotation. Source: EC rhodopsin kinase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 545

545

G protein-coupled receptor kinase 7

PRO_0000024336

Propeptide

546 – 548

Removed in mature form By similarity

PRO_0000024337

Regions

Domain

54 – 171

118

RGS

Domain

186 – 449

264

Protein kinase

Domain

450 – 515

AGC-kinase C-terminal

Nucleotide binding

192 – 200

ATP By similarity

Sites

Active site

311

Proton acceptor By similarity

Binding site

215

ATP By similarity

Amino acid modifications

Modified residue

Phosphoserine; by PKA By similarity

Modified residue

545

Cysteine methyl ester Potential

Lipidation

545

S-geranylgeranyl cysteine Potential

Sequences

Sequence

Length

Mass (Da)

Tools

Q9Z2G7 [UniParc].

Last modified May 1, 1999. Version 1.
Checksum: 092C5BFA5A42A200
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FASTA

548

61,993

        10         20         30         40         50         60 
MDMGGLDNLI ANTAYLQARK TDSDSRELQR RRRSLALPGP QGCAELRQSL SPHFHSLCEQ 

        70         80         90        100        110        120 
QPIGRRLFRD FLATVPKYSQ AVAFLEDVQN WELAEEGPAK TSTLQQLAAT CARDPGPQSF 

       130        140        150        160        170        180 
LSQDLATKCR AASTDEERKT LVEQAKAETM SFLQEQPFQD FLASPFYDRF LQWKLFEMQP 

       190        200        210        220        230        240 
VSDKYFTEFR VLGKGGFGEV CAVQVRNTGK MYACKKLDKK RLKKKGGEKM ALLEKEILEK 

       250        260        270        280        290        300 
VNSPFIVSLA YAFESKTHLC LVMSLMNGGD LKFHIYNVGT RGLAMSRVIF YTAQMTCGVL 

       310        320        330        340        350        360 
HLHGLGIVYR DLKPENVLLD DLGNCRLSDL GLAVEVQDDK PITQRAGTNG YMAPEILMDK 

       370        380        390        400        410        420 
ASYSYPVDWF AMGCSIYEMV AGRTPFKDFK EKVSKEDLKE RTMKDEVAFH HENFTEETKD 

       430        440        450        460        470        480 
ICRLFLAKKP EQRLGSREKA DDPRKHPFFQ TVNFPRLEAG LVEPPFVPDP SVVYAKDVDE 

       490        500        510        520        530        540 
IDDFSEVRGV EFDDKDKQFF QRFSTGAVPV AWQEEIIETG LFEELNDPNR PSGDGKGDSS 


KSGVCLLL

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References

[1]

"The cloning of GRK7, a candidate cone opsin kinase, from cone- and rod-dominant mammalian retinas."
Weiss E.R., Raman D., Shirakawa S., Ducceschi M.S., Bertram P.T., Wong F., Kraft T.W., Osawa S.
Mol. Vis. 4:27-27(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AUTOPHOSPHORYLATION.
Tissue: Retina.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF063016 mRNA. Translation: AAC95001.1.
3D structure databases
ProteinModelPortal	Q9Z2G7.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG004532.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR000239. GPCR_kinase. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR000342. Regulat_G_prot_signal. IPR016137. Regulat_G_prot_signal_superfam. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. PF00615. RGS. 1 hit. [Graphical view]
PRINTS	PR00717. GPCRKINASE.
SMART	SM00315. RGS. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit. SSF48097. Regulat_G_prot_signal_superfam. 1 hit.
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS50132. RGS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GRK7_SPETR

Accession

Primary (citable) accession number: Q9Z2G7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	May 1, 1999
Last modified:	April 3, 2013
This is version 79 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Phylogenomic databases

Family and domain databases

Entry information

Relevant documents