Reviewed, UniProtKB/Swiss-Prot Q9Z2G7 (GRK7_SPETR)

Names and origin

Protein names

Recommended name:
    G protein-coupled receptor kinase 7
    EC=2.7.11.16
Alternative name(s):
    G protein-coupled receptor kinase GRK7

Gene names

Name:

GRK7

Organism

Spermophilus tridecemlineatus (Thirteen-lined ground squirrel)

Taxonomic identifier

43179 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Sciuridae › Xerinae › Marmotini › Spermophilus

Protein attributes

Sequence length	548 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Phosphorylates cone opsins thereby initiating their deactivation.
Catalytic activity	ATP + [G-protein-coupled receptor] = ADP + [G-protein-coupled receptor] phosphate.
Subcellular location	Membrane; Lipid-anchor.
Tissue specificity	Retina. Cones and rod.
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. GPRK subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 protein kinase domain. Contains 1 RGS domain.

Ontologies

Keywords
Biological process	Sensory transduction Vision
Cellular component	Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Lipoprotein Methylation Prenylation
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro response to stimulus Inferred from electronic annotation. Source: UniProtKB-KW signal transduction Inferred from electronic annotation. Source: InterPro visual perception Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	anchored to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW G-protein coupled receptor kinase activity Inferred from electronic annotation. Source: EC signal transducer activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 545	545	G protein-coupled receptor kinase 7		PRO_0000024336
	Propeptide	546 – 548	3	Removed in mature form By similarity		PRO_0000024337
Regions
	Domain	54 – 171	118	RGS
	Domain	186 – 449	264	Protein kinase
	Domain	450 – 515	66	AGC-kinase C-terminal
	Nucleotide binding	192 – 200	9	ATP By similarity
Sites
	Active site	311	1	Proton acceptor By similarity
	Binding site	215	1	ATP By similarity
Amino acid modifications
	Modified residue	545	1	Cysteine methyl ester Potential
	Lipidation	545	1	S-geranylgeranyl cysteine Potential

Sequences

	Sequence		Length	Mass (Da)
	Q9Z2G7-1 [UniParc]. Last modified May 1, 1999. Version 1. Checksum: 092C5BFA5A42A200	FASTA	548	61,993
	10 20 30 40 50 60 MDMGGLDNLI ANTAYLQARK TDSDSRELQR RRRSLALPGP QGCAELRQSL SPHFHSLCEQ 70 80 90 100 110 120 QPIGRRLFRD FLATVPKYSQ AVAFLEDVQN WELAEEGPAK TSTLQQLAAT CARDPGPQSF 130 140 150 160 170 180 LSQDLATKCR AASTDEERKT LVEQAKAETM SFLQEQPFQD FLASPFYDRF LQWKLFEMQP 190 200 210 220 230 240 VSDKYFTEFR VLGKGGFGEV CAVQVRNTGK MYACKKLDKK RLKKKGGEKM ALLEKEILEK 250 260 270 280 290 300 VNSPFIVSLA YAFESKTHLC LVMSLMNGGD LKFHIYNVGT RGLAMSRVIF YTAQMTCGVL 310 320 330 340 350 360 HLHGLGIVYR DLKPENVLLD DLGNCRLSDL GLAVEVQDDK PITQRAGTNG YMAPEILMDK 370 380 390 400 410 420 ASYSYPVDWF AMGCSIYEMV AGRTPFKDFK EKVSKEDLKE RTMKDEVAFH HENFTEETKD 430 440 450 460 470 480 ICRLFLAKKP EQRLGSREKA DDPRKHPFFQ TVNFPRLEAG LVEPPFVPDP SVVYAKDVDE 490 500 510 520 530 540 IDDFSEVRGV EFDDKDKQFF QRFSTGAVPV AWQEEIIETG LFEELNDPNR PSGDGKGDSS KSGVCLLL

References

[1]

"The cloning of GRK7, a candidate cone opsin kinase, from cone- and rod-dominant mammalian retinas." Weiss E.R., Raman D., Shirakawa S., Ducceschi M.S., Bertram P.T., Wong F., Kraft T.W., Osawa S. Mol. Vis. 4:27-27(1998) [PubMed: 9852166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Retina.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF063016 mRNA. Translation: AAC95001.1.
3D structure databases
HSSP	HSSP built from PDB template 1GZK based on UniProtKB P31751.
ModBase	Search...
Protein-protein interaction databases
STRING	Q9Z2G7.
Phylogenomic databases
HOVERGEN	Q9Z2G7.
Enzyme and pathway databases
BRENDA	2.7.11.16. 277892.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR000239. GPCR_kinase. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR000342. Regulat_G_prot_signal. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. PF00615. RGS. 1 hit. [Graphical view]
PRINTS	PR00717. GPCRKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00315. RGS. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS50132. RGS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GRK7_SPETR

Accession

Primary (citable) accession number: Q9Z2G7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	May 1, 1999
Last modified:	October 13, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)