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Protein

Protein sel-1 homolog 1

Gene

Sel1l

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins (PubMed:25066055, PubMed:24453213). Enhances SYVN1 stability (PubMed:24453213). Plays a role in LPL maturation and secretion (PubMed:25066055). Required for normal differentiation of the pancreas epithelium, and for normal exocrine function and survival of pancreatic cells (PubMed:20170518, PubMed:24453213). May play a role in Notch signaling (PubMed:20170518).3 Publications

GO - Biological processi

  • ERAD pathway Source: UniProtKB
  • Notch signaling pathway Source: UniProtKB-KW
  • protein secretion Source: UniProtKB
  • response to endoplasmic reticulum stress Source: MGI
  • retrograde protein transport, ER to cytosol Source: MGI
  • triglyceride metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Notch signaling pathway

Enzyme and pathway databases

ReactomeiR-MMU-1912420. Pre-NOTCH Processing in Golgi.
R-MMU-5358346. Hedgehog ligand biogenesis.
R-MMU-901032. ER Quality Control Compartment (ERQC).

Names & Taxonomyi

Protein namesi
Recommended name:
Protein sel-1 homolog 1
Alternative name(s):
Suppressor of lin-12-like protein 1
Short name:
Sel-1L
Gene namesi
Name:Sel1l
Synonyms:Sel1h
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1329016. Sel1l.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini22 – 734713LumenalSequence analysisAdd
BLAST
Transmembranei735 – 75521HelicalSequence analysisAdd
BLAST
Topological domaini756 – 79035CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

Pathology & Biotechi

Disruption phenotypei

Taxomifen-inducible gene disruption in adult mice leads to premature death within 3 weeks after the onset of taxomifen treatment. Mice progressively loose weight and become moribund despite increased food intake and normal blood glucose levels, suggesting nutrient maladsorption. After eight days of treatment, the pancreas was diffusely dark red and soft, suggesting severe pancreas atrophy. Still, the endocrine parts of the pancreas were not affected. Pancreas weight was about half of that of wild-type, the size of secretory zymogen granules was reduced and pancreatic lipase and alpha-amylase levels were strongly reduced. Besides, the morphology of the endoplasmic reticulum in pancreas acinar cells was abnormal, with swollen and fragmented cisternae. Likewise, SYVN1 protein levels are decreased, while those of other ERAD markers are increased (PubMed:24453213). Adipocyte-specific gene disruption does not give rise to any obvious phenotype when mice are kept on a low-fat diet. Mutant mice are resistant to diet-induced obesity when kept on a high-fat diet, in spite of normal food intake and physical activity. Mutant mice show dramatically reduced accumulation of fat mass relative to wild-type, while lean mass is not affected. Intriguingly, mutant mice display enlarged livers that develop steatosis and increased triglyceride levels. Mutant mice display increased fasting serum triglyceride and insulin levels. Likewise, mutant mice display hypertriglyceridemia after feeding, especially on a high-fat diet. In spite of increased cellular LPL levels, LPL secretion is reduced by 80 to 90% (PubMed:25066055).2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi512 – 5132GG → KK: Abolishes homodimerization. 1 Publication
Mutagenesisi515 – 5162IL → AA: Abolishes homodimerization; when associated with A-519. 1 Publication
Mutagenesisi519 – 5191Y → A: Abolishes homodimerization; when associated with 515-A-A-516. 1 Publication
Mutagenesisi521 – 5211L → A: Abolishes homodimerization. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2121Sequence analysisAdd
BLAST
Chaini22 – 790769Protein sel-1 homolog 1PRO_0000022296Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei64 – 641PhosphoserineBy similarity
Disulfide bondi123 ↔ 149PROSITE-ProRule annotation
Disulfide bondi137 ↔ 164PROSITE-ProRule annotation
Glycosylationi191 – 1911N-linked (GlcNAc...)Sequence analysis
Glycosylationi213 – 2131N-linked (GlcNAc...)Sequence analysis
Glycosylationi268 – 2681N-linked (GlcNAc...)Sequence analysis
Glycosylationi427 – 4271N-linked (GlcNAc...)Sequence analysis
Glycosylationi604 – 6041N-linked (GlcNAc...)Sequence analysis

Post-translational modificationi

N-glycosylated.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiQ9Z2G6.
PaxDbiQ9Z2G6.
PRIDEiQ9Z2G6.

PTM databases

PhosphoSiteiQ9Z2G6.

Expressioni

Tissue specificityi

Highly expressed in pancreas, white adipose tissue, liver and spleen (at protein level) (PubMed:25066055, PubMed:24453213). Detected in heart, brain, spleen, lung, liver, kidney and testis (PubMed:9858735).3 Publications

Developmental stagei

First detected at 12.5 dpc in a small number of pancreas epithelial cells. Highly expressed in embryonic pancreas epithelium at later stages of embryonic development.1 Publication

Gene expression databases

BgeeiENSMUSG00000020964.
CleanExiMM_SEL1L.
ExpressionAtlasiQ9Z2G6. baseline and differential.
GenevisibleiQ9Z2G6. MM.

Interactioni

Subunit structurei

Homodimer and homooligomer (PubMed:27064360). Interacts with SYVN1; the interaction is direct (PubMed:25066055, PubMed:27064360). Part of a complex containing SEL1L, SYVN1 and DERL2. May form a complex with ERLEC1, HSPA5, OS9, and SYVN1. Interacts with FOXRED2 and EDEM1 (By similarity). Interacts with LPL and LMF1; may stabilize the complex formed by LPL and LMF1 and thereby promote the export of LPL dimers (PubMed:25066055).By similarity2 Publications

Protein-protein interaction databases

BioGridi203153. 5 interactions.
DIPiDIP-61846N.
IntActiQ9Z2G6. 7 interactions.
MINTiMINT-4134046.
STRINGi10090.ENSMUSP00000021347.

Structurei

Secondary structure

1
790
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi354 – 36512Combined sources
Helixi369 – 38113Combined sources
Helixi390 – 40213Combined sources
Helixi406 – 41712Combined sources
Beta strandi421 – 4233Combined sources
Helixi427 – 43913Combined sources
Helixi443 – 45412Combined sources
Beta strandi457 – 4593Combined sources
Helixi463 – 47513Combined sources
Helixi479 – 49012Combined sources
Beta strandi493 – 4964Combined sources
Helixi499 – 51012Combined sources
Helixi517 – 5215Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5B26X-ray2.60A/B/C/D348-533[»]
ProteinModelPortaliQ9Z2G6.
SMRiQ9Z2G6. Positions 112-164, 186-713.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini118 – 16649Fibronectin type-IIPROSITE-ProRule annotationAdd
BLAST
Repeati179 – 21436Sel1-like 1Add
BLAST
Repeati215 – 25036Sel1-like 2Add
BLAST
Repeati251 – 28636Sel1-like 3Add
BLAST
Repeati287 – 32236Sel1-like 4Add
BLAST
Repeati369 – 40537Sel1-like 5Add
BLAST
Repeati406 – 44237Sel1-like 6Add
BLAST
Repeati443 – 47836Sel1-like 7Add
BLAST
Repeati479 – 51436Sel1-like 8Add
BLAST
Repeati515 – 55036Sel1-like 9Add
BLAST
Repeati623 – 65836Sel1-like 10Add
BLAST
Repeati660 – 69536Sel1-like 11Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni23 – 733711Interaction with ERLEC1, OS9 and SYVN1By similarityAdd
BLAST
Regioni348 – 533186Important for homodimerization and oligomerization1 PublicationAdd
BLAST
Regioni639 – 71981Interaction with SYVN11 PublicationAdd
BLAST
Regioni734 – 79057Mediates retention in the endoplasmic reticulumBy similarityAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi766 – 78924Pro-richAdd
BLAST

Sequence similaritiesi

Belongs to the sel-1 family.Curated
Contains 1 fibronectin type-II domain.PROSITE-ProRule annotation
Contains 11 Sel1-like repeats.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1550. Eukaryota.
COG0790. LUCA.
GeneTreeiENSGT00530000063170.
HOVERGENiHBG057766.
InParanoidiQ9Z2G6.
KOiK14026.
OMAiNHTKAME.
OrthoDBiEOG091G0315.
TreeFamiTF315257.

Family and domain databases

CDDicd00062. FN2. 1 hit.
Gene3Di1.25.40.10. 3 hits.
2.10.10.10. 1 hit.
InterProiIPR000562. FN_type2_col-bd.
IPR013806. Kringle-like.
IPR006597. Sel1-like.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF00040. fn2. 1 hit.
PF08238. Sel1. 12 hits.
[Graphical view]
SMARTiSM00059. FN2. 1 hit.
SM00671. SEL1. 11 hits.
[Graphical view]
SUPFAMiSSF57440. SSF57440. 1 hit.
PROSITEiPS00023. FN2_1. 1 hit.
PS51092. FN2_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9Z2G6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQVRVRLSLL LLCAVLLGSA AATSDDKTNQ DDSLDSKSSL PTDESVKDHT
60 70 80 90 100
TTGKVVAGQI FVDSEEAEVE SLLQDEEDSS KTQEEEISFL ESPNPSSKTY
110 120 130 140 150
EELKRVRKPV LTAIEGTAHG EPCHFPFLFL DKEYDECTSD GREDGRLWCA
160 170 180 190 200
TTYDYKTDEK WGFCETEEDA AKRRQMQEAE MIYQAGMKIL NGSNRKSQKR
210 220 230 240 250
EAYRYLQKAA GMNHTKALER VSYALLFGDY LTQNIQAAKE MFEKLTEEGS
260 270 280 290 300
PKGQTGLGFL YASGLGVNSS QAKALVYYTF GALGGNLIAH MILGYRYWAG
310 320 330 340 350
IGVLQSCESA LTHYRLVANH VASDISLTGG SVVQRIRLPD EVENPGMNSG
360 370 380 390 400
MLEEDLIQYY QFLAEKGDVQ AQVGLGQLHL HGGRGVEQNH QRAFDYFNLA
410 420 430 440 450
ANAGNSHAMA FLGKMYSEGS DIVPQSNETA LHYFKKAADM GNPVGQSGLG
460 470 480 490 500
MAYLYGRGVQ VNYDLALKYF QKAAEQGWVD GQLQLGSMYY NGIGVKRDYK
510 520 530 540 550
QALKYFNLAS QGGHILAFYN LAQMHASGTG VMRSCHTAVE LFKNVCERGR
560 570 580 590 600
WSERLMTAYN SYKDEDYNAA VVQYLLLAEQ GYEVAQSNAA FILDQREATI
610 620 630 640 650
VGENETYPRA LLHWNRAASQ GYTVARIKLG DYHFYGFGTD VDYETAFIHY
660 670 680 690 700
RLASEQQHSA QAMFNLGYMH EKGLGIKQDI HLAKRFYDMA AEASPDAQVP
710 720 730 740 750
VFLALCKLGV VYFLQYIREA NIRDLFTQLD MDQLLGPEWD LYLMTIIALL
760 770 780 790
LGTVIAYRQR QHQDIPVPRP PGPRPAPPQQ EGPPEQQPPQ
Length:790
Mass (Da):88,340
Last modified:April 27, 2001 - v2
Checksum:i47869F2AFB59B936
GO
Isoform 2 (identifier: Q9Z2G6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     116-166: GTAHGEPCHFPFLFLDKEYDECTSDGREDGRLWCATTYDYKTDEKWGFCET → A

Note: No experimental confirmation available.
Show »
Length:740
Mass (Da):82,457
Checksum:iB51D80A451C28AB1
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei116 – 16651GTAHG…GFCET → A in isoform 2. 1 PublicationVSP_004384Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF063095 mRNA. Translation: AAD05210.1.
AK005023 mRNA. Translation: BAB23750.1.
CCDSiCCDS26091.1. [Q9Z2G6-1]
CCDS49137.1. [Q9Z2G6-2]
RefSeqiNP_001034178.1. NM_001039089.1. [Q9Z2G6-1]
NP_035474.1. NM_011344.2. [Q9Z2G6-2]
UniGeneiMm.250605.

Genome annotation databases

EnsembliENSMUST00000021347; ENSMUSP00000021347; ENSMUSG00000020964. [Q9Z2G6-1]
ENSMUST00000167466; ENSMUSP00000129384; ENSMUSG00000020964. [Q9Z2G6-2]
GeneIDi20338.
KEGGimmu:20338.
UCSCiuc007oky.1. mouse. [Q9Z2G6-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF063095 mRNA. Translation: AAD05210.1.
AK005023 mRNA. Translation: BAB23750.1.
CCDSiCCDS26091.1. [Q9Z2G6-1]
CCDS49137.1. [Q9Z2G6-2]
RefSeqiNP_001034178.1. NM_001039089.1. [Q9Z2G6-1]
NP_035474.1. NM_011344.2. [Q9Z2G6-2]
UniGeneiMm.250605.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
5B26X-ray2.60A/B/C/D348-533[»]
ProteinModelPortaliQ9Z2G6.
SMRiQ9Z2G6. Positions 112-164, 186-713.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi203153. 5 interactions.
DIPiDIP-61846N.
IntActiQ9Z2G6. 7 interactions.
MINTiMINT-4134046.
STRINGi10090.ENSMUSP00000021347.

PTM databases

PhosphoSiteiQ9Z2G6.

Proteomic databases

MaxQBiQ9Z2G6.
PaxDbiQ9Z2G6.
PRIDEiQ9Z2G6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021347; ENSMUSP00000021347; ENSMUSG00000020964. [Q9Z2G6-1]
ENSMUST00000167466; ENSMUSP00000129384; ENSMUSG00000020964. [Q9Z2G6-2]
GeneIDi20338.
KEGGimmu:20338.
UCSCiuc007oky.1. mouse. [Q9Z2G6-1]

Organism-specific databases

CTDi6400.
MGIiMGI:1329016. Sel1l.

Phylogenomic databases

eggNOGiKOG1550. Eukaryota.
COG0790. LUCA.
GeneTreeiENSGT00530000063170.
HOVERGENiHBG057766.
InParanoidiQ9Z2G6.
KOiK14026.
OMAiNHTKAME.
OrthoDBiEOG091G0315.
TreeFamiTF315257.

Enzyme and pathway databases

ReactomeiR-MMU-1912420. Pre-NOTCH Processing in Golgi.
R-MMU-5358346. Hedgehog ligand biogenesis.
R-MMU-901032. ER Quality Control Compartment (ERQC).

Miscellaneous databases

ChiTaRSiSel1l. mouse.
PROiQ9Z2G6.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020964.
CleanExiMM_SEL1L.
ExpressionAtlasiQ9Z2G6. baseline and differential.
GenevisibleiQ9Z2G6. MM.

Family and domain databases

CDDicd00062. FN2. 1 hit.
Gene3Di1.25.40.10. 3 hits.
2.10.10.10. 1 hit.
InterProiIPR000562. FN_type2_col-bd.
IPR013806. Kringle-like.
IPR006597. Sel1-like.
IPR011990. TPR-like_helical_dom.
[Graphical view]
PfamiPF00040. fn2. 1 hit.
PF08238. Sel1. 12 hits.
[Graphical view]
SMARTiSM00059. FN2. 1 hit.
SM00671. SEL1. 11 hits.
[Graphical view]
SUPFAMiSSF57440. SSF57440. 1 hit.
PROSITEiPS00023. FN2_1. 1 hit.
PS51092. FN2_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSE1L1_MOUSE
AccessioniPrimary (citable) accession number: Q9Z2G6
Secondary accession number(s): Q9DBD8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: September 7, 2016
This is version 136 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Gene disruption after residue 465 and replacement of the C-terminus with a beta-galactosidase-neomycin reporter gene construct leads to complete embryonic lethality; most die before 13.5 dpc. Only 5% of the embryos are viable at 15.5 dpc. Mutant embryos display defects in the differentiation of the pancreas epithelium. The defects in pancreas differentiation can be alleviated by pharmacological inhibition of Notch signaling (in vitro).1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.